TY - JOUR AU - Igaz, Nóra AU - Kovács, Dávid AU - Rázga, Zsolt AU - Kónya, Zoltán AU - Boros, Imre Miklós AU - Csontné Kiricsi, Mónika TI - Modulating chromatin structure and DNA accessibility by deacetylase inhibition enhances the anti-cancer activity of silver nanoparticles JF - COLLOIDS AND SURFACES B: BIOINTERFACES J2 - COLLOID SURFACE B VL - 146 PY - 2016 SP - 670 EP - 677 PG - 8 SN - 0927-7765 DO - 10.1016/j.colsurfb.2016.07.004 UR - https://m2.mtmt.hu/api/publication/3097004 ID - 3097004 AB - Abstract Histone deacetylase (HDAC) inhibitors are considered as novel therapeutic agents inducing cell cycle arrest and apoptotic cell death in various cancer cells. Inhibition of deacetylase activity results in a relaxed chromatin structure thereby rendering the genetic material more vulnerable to DNA targeting agents that could be exploited by combinational cancer therapy. The unique potential of silver nanoparticles (AgNPs) in tumor therapy relies on the generation of reactive radicals which trigger oxidative stress, DNA damage and apoptosis in cancer cells. The revolutionary application of AgNPs as chemotherapeutical drugs seems very promising, nevertheless the exact molecular mechanisms of AgNP action in combination with other anti-cancer agents have yet to be elucidated in details before clinical administrations. As a step towards this we investigated the combinational effect of HDAC inhibition and AgNP administration in HeLa cervical cancer cells. We identified synergistic inhibition of cancer cell growth and migration upon combinational treatments. Here we report that the HDAC inhibitor Trichostatin A enhances the DNA targeting capacity and apoptosis inducing efficacy of AgNPs most probably due to its effect on chromatin condensation. These results point to the potential benefits of combinational application of HDAC inhibitors and AgNPs in novel cancer medication protocols. LA - English DB - MTMT ER - TY - JOUR AU - Kovács, Dávid AU - Igaz, Nóra AU - Keskeny, Csilla AU - Bélteky, Péter AU - Tóth, Tímea AU - Molnár-Gáspár, Renáta AU - Madarász, Dániel AU - Rázga, Zsolt AU - Kónya, Zoltán AU - Boros, Imre Miklós AU - Csontné Kiricsi, Mónika TI - Silver nanoparticles defeat p53-positive and p53-negative osteosarcoma cells by triggering mitochondrial stress and apoptosis JF - SCIENTIFIC REPORTS J2 - SCI REP VL - 6 PY - 2016 PG - 13 SN - 2045-2322 DO - 10.1038/srep27902 UR - https://m2.mtmt.hu/api/publication/3079142 ID - 3079142 AB - Loss of function of the tumour suppressor p53 observed frequently in human cancers challenges the drug-induced apoptotic elimination of cancer cells from the body. This phenomenon is a major concern and provides much of the impetus for current attempts to develop a new generation of anticancer drugs capable of provoking apoptosis in a p53-independent manner. Since silver nanoparticles (AgNPs) possess unique cytotoxic features, we examined, whether their activity could be exploited to kill tumour suppressor-deficient cancer cells. Therefore, we investigated the effects of AgNPs on osteosarcoma cells of different p53 genetic backgrounds. As particle diameters might influence the molecular mechanisms leading to AgNP-induced cell death we applied 5 nm and 35 nm sized citrate-coated AgNPs. We found that both sized AgNPs targeted mitochondria and induced apoptosis in wild-type p53-containing U2Os and p53-deficient Saos-2 cells. According to our findings AgNPs are able to kill osteosarcoma cells independently from their actual p53 status and induce p53-independent cancer cell apoptosis. This feature renders AgNPs attractive candidates for novel chemotherapeutic approaches. LA - English DB - MTMT ER - TY - JOUR AU - Kozma, Gábor AU - Rónavári, Andrea AU - Kónya, Zoltán AU - Kukovecz, Ákos TI - Environmentally benign synthesis methods of zero valent iron nanoparticles JF - ACS SUSTAINABLE CHEMISTRY & ENGINEERING J2 - ACS SUSTAIN CHEM ENG VL - 4 PY - 2016 IS - 1 SP - 291 EP - 297 PG - 7 SN - 2168-0485 DO - 10.1021/acssuschemeng.5b01185 UR - https://m2.mtmt.hu/api/publication/2980124 ID - 2980124 N1 - Department of Applied and Environmental Chemistry, University of Szeged, Rerrich Béla tér 1., Szeged, H-6720, Hungary MTA-SZTE Lendület Porous Nanocomposites Research Group, Rerrich Béla tér 1., Szeged, H-6720, Hungary MTA-SZTE Reaction Kinetics and Surface Chemistry Research Group, Rerrich Béla tér 1., Szeged, H-6720, Hungary Cited By :22 Export Date: 24 September 2019 Correspondence Address: Kukovecz, Á.; Department of Applied and Environmental Chemistry, University of Szeged, Rerrich Béla tér 1., Hungary; email: kakos@chem.u-szeged.hu LA - English DB - MTMT ER - TY - JOUR AU - Rónavári, Andrea AU - Kovács, Dávid AU - Vágvölgyi, Csaba AU - Kónya, Zoltán AU - Csontné Kiricsi, Mónika AU - Pfeiffer, Ilona TI - Ion exchange defines the biological activity of titanate nanotubes JF - JOURNAL OF BASIC MICROBIOLOGY J2 - J BASIC MICROB VL - 56 PY - 2016 IS - 5 SP - 557 EP - 565 PG - 9 SN - 0233-111X DO - 10.1002/jobm.201500742 UR - https://m2.mtmt.hu/api/publication/3045389 ID - 3045389 AB - One-dimensional titanate nanotubes (TiONTs) were subjected to systematic ion exchange to determine the impact of these modifications on biological activities. Ion exchanged TiONTs (with Ag, Mg, Bi, Sb, Ca, K, Sr, Fe, and Cu ions) were successfully synthesized and the presence of the substituted ions was verified by energy dispersive X-ray spectroscopy (EDS). A complex screening was carried out to reveal differences in toxicity to human cells, as well as in antibacterial, antifungal, and antiviral activities between the various modified nanotubes. Our results demonstrated that Ag ion exchanged TiONTs exerted potent antibacterial and antifungal effects against all examined microbial species but were ineffective on viruses. Surprisingly, the antibacterial activity of Cu/TiONTs was restricted to Micrococcus luteus. Most ion exchanged TiONTs did not show antimicrobial activity against the tested bacterial and fungal species. Incorporation of various ions into nanotube architectures lead to mild, moderate, or even to a massive loss of human cell viability; therefore, this type of biological effect exerted by TiONTs can be greatly modulated by ion exchange. These findings further emphasize the contribution of ion exchange in determining not only the physical and chemical characteristics but also the bioactivity of TiONT against different types of living cells. LA - English DB - MTMT ER -