@article{MTMT:2136659, title = {Assigning DYW-type PPR proteins to RNA editing sites in the funariid mosses Physcomitrella patens and Funaria hygrometrica}, url = {https://m2.mtmt.hu/api/publication/2136659}, author = {Rudinger, M and Szövényi, Péter and Rensing, SA and Knoop, V}, doi = {10.1111/j.1365-313X.2011.04600.x}, journal-iso = {PLANT J}, journal = {PLANT JOURNAL}, volume = {67}, unique-id = {2136659}, issn = {0960-7412}, abstract = {The plant-specific pentatricopeptide repeat (PPR) proteins with variable PPR repeat lengths (PLS-type) and protein extensions up to the carboxyterminal DYW domain have received attention as specific recognition factors for the C-to-U type of RNA editing events in plant organelles. Here, we report a DYW-protein knockout in the model plant Physcomitrella patens specifically affecting mitochondrial RNA editing positions cox1eU755SL and rps14eU137SL. Assignment of DYW proteins and RNA editing sites might best be corroborated by data from a taxon with a slightly different, yet similarly manageable low number of editing sites and DYW proteins. To this end we investigated the mitochondrial editing status of the related funariid moss Funaria hygrometrica. We find that: (i) Funaria lacks three mitochondrial RNA editing positions present in Physcomitrella, (ii) that F. hygrometrica cDNA sequence data identify nine DYW proteins as clear orthologues of their P. patens counterparts, and (iii) that the 'missing' 10th DYW protein in F. hygrometrica is responsible for two mitochondrial editing sites in P. patens lacking in F. hygrometrica (nad3eU230SL, nad4eU272SL). Interestingly, the third site of RNA editing missing in F. hygrometrica (rps14eU137SL) is addressed by the DYW protein characterized here and the presence of its orthologue in F. hygrometrica is explained through its simultaneous action on site cox1eU755SL conserved in both mosses.}, keywords = {GENE; DOMAIN; GENOME; ARABIDOPSIS-THALIANA; CHLOROPLASTS; REVEALS; MULTIPLE SITES; PLANT ORGANELLES; MITOCHONDRIAL TRANSCRIPTS; PENTATRICOPEPTIDE REPEAT PROTEIN; targeted knockouts; plant organelle RNA editing; bryophyte transcriptome; virtual transcripts; pentatricopeptide repeat proteins}, year = {2011}, eissn = {1365-313X}, pages = {370-380} } @article{MTMT:1271158, title = {Mapping of promoters for the nucleus encoded plastid RNA polymerase (NEP) in the iojap maize mutant}, url = {https://m2.mtmt.hu/api/publication/1271158}, author = {Silhavy, Dániel and Maliga, Pál}, doi = {10.1007/s002940050345}, journal-iso = {CURR GENET}, journal = {CURRENT GENETICS}, volume = {33}, unique-id = {1271158}, issn = {0172-8083}, abstract = {Plastid genes of higher plants may be transcribed by the plastid-encoded or the nucleus-encoded plastid RNA polymerases (PEP or NEP). The objective of this study was to identify NEP promoters in maize. To separate the NEP and PEP transcription activity, NEP pro meter mapping was carried out in the iojap maize mutant which lacks the PEP. We report here that atpB, an ATPase subunit gene has promoters for both NEP and PEP, while clpP, a protease subunit gene, and the rpoB operon, encoding three PEP subunit genes, are exclusively transcribed from NEP promoters. The maize NEP promoters share sequence homology around the transcription initiation site, including the ATAGAATA/GAA loose consensus identified for tobacco, suggesting conservation of the NEP transcription machinery between monocots and dicots.}, year = {1998}, eissn = {1432-0983}, pages = {340-344} } @article{MTMT:1271155, title = {The phage-type PclpP-53 plastid promoter comprises sequences downstream of the transcription initiation site}, url = {https://m2.mtmt.hu/api/publication/1271155}, author = {Sriraman, P and Silhavy, Dániel and Maliga, Pál}, doi = {10.1093/nar/26.21.4874}, journal-iso = {NUCLEIC ACIDS RES}, journal = {NUCLEIC ACIDS RESEARCH}, volume = {26}, unique-id = {1271155}, issn = {0305-1048}, abstract = {The existence of a phage-type plastid transcription machinery (NEP), related to the mitochondrial RNA polymerase, has been recognized only recently. Here we report the cis sequences required for transcription initiation by the phage-type enzyme. The promoter chosen for the study, PclpP-53, is well expressed in tobacco leaves, unlike most NEP promoters, Promoter definition was carried out in vivo, in transplastomic tobacco plants expressing a uidA reporter gene from PclpP-53 promoter derivatives. We report here that sequences from -5 to +25 (relative to the transcription initiation site) are sufficient to support specific transcription initiation, Requirement of sequences downstream of the transcription initiation site contrasts with mitochondrial promoters, which have conserved sequences predominantly upstream. The promoter defined here is conserved in liverworts and conifers, indicating that the phage-type transcription machinery appeared in plastids early on during the evolution of land plants. The PclpP-53 promoter sequences are present in rice but do not function, suggesting that PclpP-53 recognition specificity is absent in some monocots.}, year = {1998}, eissn = {1362-4962}, pages = {4874-4879} }