TY - JOUR AU - Keserű, Judit Szilvia AU - Soltész, Beáta AU - Lukács, János AU - Márton, Éva AU - Szilágyi-Bónizs, Melinda AU - Penyige, András AU - Póka, Róbert AU - Nagy, Bálint TI - Detection of cell-free, exosomal and whole blood mitochondrial DNA copy number in plasma or whole blood of patients with serous epithelial ovarian cancer. JF - JOURNAL OF BIOTECHNOLOGY J2 - J BIOTECHNOL VL - 298 PY - 2019 SP - 76 EP - 81 PG - 6 SN - 0168-1656 DO - 10.1016/j.jbiotec.2019.04.015 UR - https://m2.mtmt.hu/api/publication/30643226 ID - 30643226 AB - Ovarian tumor is one of the leading causes of cancer among women. Patients are diagnosed at an advanced stage, usually. There is a need for new specific and sensitive biomarkers. Mitochondrial DNA copy number change was observed in various cancers. Our aim was to detect mitochondrial DNA copy number in whole blood (wb-mtDNA) and in plasma (cell-free and exosome encapsulated mtDNA) in patients with serous epithelial ovarian tumor. DNA was isolated from EDTA blood and plasma obtained from 24 patients and 24 healthy controls. Exosomes were isolated from cell-free plasma, and exosome encapsulated DNA (exoDNA) was extracted. Quantitative-real-time PCR was performed with Human Mitochondrial DNA (mtDNA) Monitoring Primer Set. Kruskall‑Wallis and Mann‑Whitney U test were used for data analysis. Wb-mtDNA copy number was significantly different among healthy controls and patients in multiple comparison (p = 0.0090 considering FIGO stage independently, and p = 0.0048 considering early- and late-stage cancers). There was a significant decrease among early-stage, all advanced stage and all cancer patients (FIGO I: 32.5 ± 8.3, p = 0.0061; FIGO III + IV: 37.2 ± 13.7 p = 0.0139; FIGO I + III + IV: 35.6 ± 12.2, p = 0.0017) or FIGO III patients alone (32.8 ± 5.6, p = 0.00089) compared to healthy controls. We found significant increase in copy number in exosomal mtDNA in cancer patients (236.0 ± 499.0, p = 0.0155), advanced-stage cancer patients (333.0 ± 575.0, p = 0.0095), of FIGO III (362.0 ± 609.2, p = 0.0494), and FIGO IV (304.0 ± 585.0, p = 0.0393) patients alone but not in samples of FIGO I patients (10.0 ± 3.5, p = 0.3907). In multiple comparison the increase was significant considering early- and late-stage cancers (p = 0.0253). Cell-free mtDNA copy numbers were not increased significantly. We found the highest copy number of mtDNA in exosomes, followed by plasma and peripheral blood in late-stage cancer patients. We observed significant difference in wb-mtDNA copy number between healthy controls and both early- and late-stage cancer patients. LA - English DB - MTMT ER - TY - JOUR AU - Théry, C. AU - Witwer, K.W. AU - Aikawa, E. AU - Alcaraz, M.J. AU - Anderson, J.D. AU - Andriantsitohaina, R. AU - Antoniou, A. AU - Arab, T. AU - Archer, F. AU - Atkin-Smith, G.K. AU - Ayre, D.C. AU - Bach, J.-M. AU - Bachurski, D. AU - Baharvand, H. AU - Balaj, L. AU - Baldacchino, S. AU - Bauer, N.N. AU - Baxter, A.A. AU - Bebawy, M. AU - Beckham, C. AU - Bedina, Zavec A. AU - Benmoussa, A. AU - Berardi, A.C. AU - Bergese, P. AU - Bielska, E. AU - Blenkiron, C. AU - Bobis-Wozowicz, S. AU - Boilard, E. AU - Boireau, W. AU - Bongiovanni, A. AU - Borràs, F.E. AU - Bosch, S. AU - Boulanger, C.M. AU - Breakefield, X. AU - Breglio, A.M. AU - Brennan, M.Á. AU - Brigstock, D.R. AU - Brisson, A. AU - Broekman, M.L.D. AU - Bromberg, J.F. AU - Bryl-Górecka, P. AU - Buch, S. AU - Buck, A.H. AU - Burger, D. AU - Busatto, S. AU - Buschmann, D. AU - Bussolati, B. AU - Buzás, Edit Irén AU - Byrd, J.B. AU - Camussi, G. AU - Carter, D.R.F. AU - Caruso, S. AU - Chamley, L.W. AU - Chang, Y.-T. AU - Chaudhuri, A.D. AU - Chen, C. AU - Chen, S. AU - Cheng, L. AU - Chin, A.R. AU - Clayton, A. AU - Clerici, S.P. AU - Cocks, A. AU - Cocucci, E. AU - Coffey, R.J. AU - Cordeiro-da-Silva, A. AU - Couch, Y. AU - Coumans, F.A.W. AU - Coyle, B. AU - Crescitelli, R. AU - Criado, M.F. AU - D’Souza-Schorey, C. AU - Das, S. AU - de, Candia P. AU - De, Santana E.F. Jr. AU - De, Wever O. AU - del, Portillo H.A. AU - Demaret, T. AU - Deville, S. AU - Devitt, A. AU - Dhondt, B. AU - Di, Vizio D. AU - Dieterich, L.C. AU - Dolo, V. AU - Dominguez, Rubio A.P. AU - Dominici, M. AU - Dourado, M.R. AU - Driedonks, T.A.P. AU - Duarte, F.V. AU - Duncan, H.M. AU - Eichenberger, R.M. AU - Ekström, K. AU - EL, Andaloussi S. AU - Elie-Caille, C. AU - Erdbrügger, U. AU - Falcón-Pérez, J.M. AU - Fatima, F. AU - Fish, J.E. AU - Flores-Bellver, M. AU - Försönits, András AU - Frelet-Barrand, A. AU - Fricke, F. AU - Fuhrmann, G. AU - Gabrielsson, S. AU - Gámez-Valero, A. AU - Gardiner, C. AU - Gärtner, K. AU - Gaudin, R. AU - Gho, Y.S. AU - Giebel, B. AU - Gilbert, C. AU - Gimona, M. AU - Giusti, I. AU - Goberdhan, D.C.I. AU - Görgens, A. AU - Gorski, S.M. AU - Greening, D.W. AU - Gross, J.C. AU - Gualerzi, A. AU - Gupta, G.N. AU - Gustafson, D. AU - Handberg, A. AU - Haraszti, R.A. AU - Harrison, P. AU - Hegyesi, Hargita AU - Hendrix, A. AU - Hill, A.F. AU - Hochberg, F.H. AU - Hoffmann, K.F. AU - Holder, B. AU - Holthofer, H. AU - Hosseinkhani, B. AU - Hu, G. AU - Huang, Y. AU - Huber, V. AU - Hunt, S. AU - Ibrahim, A.G.-E. AU - Ikezu, T. AU - Inal, J.M. AU - Isin, M. AU - Ivanova, A. AU - Jackson, H.K. AU - Jacobsen, S. AU - Jay, S.M. AU - Jayachandran, M. AU - Jenster, G. AU - Jiang, L. AU - Johnson, S.M. AU - Jones, J.C. AU - Jong, A. AU - Jovanovic-Talisman, T. AU - Jung, S. AU - Kalluri, R. AU - Kano, S.-I. AU - Kaur, S. AU - Kawamura, Y. AU - Keller, E.T. AU - Khamari, Delaram AU - Khomyakova, E. AU - Khvorova, A. AU - Kierulf, P. AU - Kim, K.P. AU - Kislinger, T. AU - Klingeborn, M. AU - Klinke, D.J. II AU - Kornek, M. AU - Kosanović, M.M. AU - Kovács, Árpád Ferenc AU - Krämer-Albers, E.-M. AU - Krasemann, S. AU - Krause, M. AU - Kurochkin, I.V. AU - Kusuma, G.D. AU - Kuypers, S. AU - Laitinen, S. AU - Langevin, S.M. AU - Languino, L.R. AU - Lannigan, J. AU - Lässer, C. AU - Laurent, L.C. AU - Lavieu, G. AU - Lázaro-Ibáñez, E. AU - Le, Lay S. AU - Lee, M.-S. AU - Lee, Y.X.F. AU - Lemos, D.S. AU - Lenassi, M. AU - Leszczynska, A. AU - Li, I.T.S. AU - Liao, K. AU - Libregts, S.F. AU - Ligeti, Erzsébet AU - Lim, R. AU - Lim, S.K. AU - Linē, A. AU - Linnemannstöns, K. AU - Llorente, A. AU - Lombard, C.A. AU - Lorenowicz, M.J. AU - Lőrincz, Márton Ákos AU - Lötvall, J. AU - Lovett, J. AU - Lowry, M.C. AU - Loyer, X. AU - Lu, Q. AU - Lukomska, B. AU - Lunavat, T.R. AU - Maas, S.L.N. AU - Malhi, H. AU - Marcilla, A. AU - Mariani, J. AU - Mariscal, J. AU - Martens-Uzunova, E.S. AU - Martin-Jaular, L. AU - Martinez, M.C. AU - Martins, V.R. AU - Mathieu, M. AU - Mathivanan, S. AU - Maugeri, M. AU - McGinnis, L.K. AU - McVey, M.J. AU - Meckes, D.G. Jr AU - Meehan, K.L. AU - Mertens, I. AU - Minciacchi, V.R. AU - Möller, A. AU - Møller, Jørgensen M. AU - Morales-Kastresana, A. AU - Morhayim, J. AU - Mullier, F. AU - Muraca, M. AU - Musante, L. AU - Mussack, V. AU - Muth, D.C. AU - Myburgh, K.H. AU - Najrana, T. AU - Nawaz, M. AU - Nazarenko, I. AU - Nejsum, P. AU - Neri, C. AU - Neri, T. AU - Nieuwland, R. AU - Nimrichter, L. AU - Nolan, J.P. AU - Nolte-’t, Hoen E.N.M. AU - Hooten, N.N. AU - O’Driscoll, L. AU - O’Grady, T. AU - O’Loghlen, A. AU - Ochiya, T. AU - Olivier, M. AU - Ortiz, A. AU - Ortiz, L.A. AU - Osteikoetxea, Xabier AU - Ostegaard, O. AU - Ostrowski, M. AU - Park, J. AU - Pegtel, D.M. AU - Peinado, H. AU - Perut, F. AU - Pfaffl, M.W. AU - Phinney, D.G. AU - Pieters, B.C.H. AU - Pink, R.C. AU - Pisetsky, D.S. AU - Pogge, von Strandmann E. AU - Polakovicova, I. AU - Poon, I.K.H. AU - Powell, B.H. AU - Prada, I. AU - Pulliam, L. AU - Quesenberry, P. AU - Radeghieri, A. AU - Raffai, R.L. AU - Raimondo, S. AU - Rak, J. AU - Ramirez, M.I. AU - Raposo, G. AU - Rayyan, M.S. AU - Regev-Rudzki, N. AU - Ricklefs, F.L. AU - Robbins, P.D. AU - Roberts, D.D. AU - Rodrigues, S.C. AU - Rohde, E. AU - Rome, S. AU - Rouschop, K.M.A. AU - Rughetti, A. AU - Russell, A.E. AU - Saá, P. AU - Sahoo, S. AU - Salas-Huenuleo, E. AU - Sánchez, C. AU - Saugstad, J.A. AU - Saul, M.J. AU - Schiffelers, R.M. AU - Schneider, R. AU - Schøyen, T.H. AU - Scott, A. AU - Shahaj, E. AU - Sharma, S. AU - Shatnyeva, O. AU - Shekari, F. AU - Shelke, G.V. AU - Shetty, A.K. AU - Shiba, K. AU - Siljander, P.R.-M. AU - Silva, A.M. AU - Skowronek, A. AU - Snyder, O.L. II AU - Soares, R.P. AU - Sódar, Barbara AU - Soekmadji, C. AU - Sotillo, J. AU - Stahl, P.D. AU - Stoorvogel, W. AU - Stott, S.L. AU - Strasser, E.F. AU - Swift, S. AU - Tahara, H. AU - Tewari, M. AU - Timms, K. AU - Tiwari, S. AU - Tixeira, R. AU - Tkach, M. AU - Toh, W.S. AU - Tomasini, R. AU - Torrecilhas, A.C. AU - Tosar, J.P. AU - Toxavidis, V. AU - Urbanelli, L. AU - Vader, P. AU - van, Balkom B.W.M. AU - van, der Grein S.G. AU - Van, Deun J. AU - van, Herwijnen M.J.C. AU - Van, Keuren-Jensen K. AU - van, Niel G. AU - van, Royen M.E. AU - van, Wijnen A.J. AU - Vasconcelos, M.H. AU - Vechetti, I.J. Jr AU - Veit, T.D. AU - Vella, L.J. AU - Velot, É. AU - Verweij, F.J. AU - Vestad, B. AU - Viñas, J.L. AU - Visnovitz, Tamás AU - Visnovitzné Dr Vukman, Krisztina AU - Wahlgren, J. AU - Watson, D.C. AU - Wauben, M.H.M. AU - Weaver, A. AU - Webber, J.P. AU - Weber, V. AU - Wehman, A.M. AU - Weiss, D.J. AU - Welsh, J.A. AU - Wendt, S. AU - Wheelock, A.M. AU - Wiener, Zoltán AU - Witte, L. AU - Wolfram, J. AU - Xagorari, A. AU - Xander, P. AU - Xu, J. AU - Yan, X. AU - Yáñez-Mó, M. AU - Yin, H. AU - Yuana, Y. AU - Zappulli, V. AU - Zarubova, J. AU - Žėkas, V. AU - Zhang, J.-Y. AU - Zhao, Z. AU - Zheng, L. AU - Zheutlin, A.R. AU - Zickler, A.M. AU - Zimmermann, P. AU - Zivkovic, A.M. AU - Zocco, D. AU - Zuba-Surma, E.K. TI - Minimal information for studies of extracellular vesicles 2018 (MISEV2018): a position statement of the International Society for Extracellular Vesicles and update of the MISEV2014 guidelines JF - JOURNAL OF EXTRACELLULAR VESICLES J2 - J EXTRACELLULAR VESICL VL - 7 PY - 2018 IS - 1 PG - 47 SN - 2001-3078 DO - 10.1080/20013078.2018.1535750 UR - https://m2.mtmt.hu/api/publication/30338008 ID - 30338008 LA - English DB - MTMT ER - TY - JOUR AU - Biró, Orsolya AU - Alasztics, Bálint AU - Molvarec, Attila AU - Joó, József Gábor AU - Nagy, Bálint AU - Rigó, János TI - Various levels of circulating exosomal total-miRNA and miR-210 hypoxamiR in different forms of pregnancy hypertension JF - PREGNANCY HYPERTENSION J2 - PREGNANCY HYPERTENS VL - 10 PY - 2017 SP - 207 EP - 212 PG - 6 SN - 2210-7789 DO - 10.1016/j.preghy.2017.09.002 UR - https://m2.mtmt.hu/api/publication/3273020 ID - 3273020 AB - Introduction: Hypertension is a common complication during pregnancy, affecting 10% of pregnant women worldwide. Several microRNA (miRNA) were shown to be involved in hypertensive disorders of pregnancy. In preeclampsia (PE), placental dysfunction causes the enhanced release of extracellular vesicle-derived miRNAs. The hypoxia-sensitive hsa-mir-210 is the most common PE-associated miRNA, but its exosomal profile has not been investigated. Objectives: Our aims were to measure exosomal total-miRNA concentration and to perform expression analysis of circulating exosomal hsa-miR-210 in women affected by chronic hypertension (CHT) gestational hypertension (GHT) or PE. Materials and methods: We collected plasma samples from women with CHT, GHT, PE (moderate: mPE and severe: sPE) and from normotensive pregnancies. Exosomal miRNAs were extracted and miRNA concentration was measured. RT-PCR was carried out with hsa-miR-210-3p-specific primers and relative expression was calculated using the comparative Ct method. Results: The total-miRNA concentration was different in the disease subgroups, and was significantly higher in mPE and sPE compared to the other groups. We found a significant difference in the relative exosomal hsa-miR-210-3p expression between all hypertensive groups compared to the normotensive samples, but significant upregulation was only observed in case of mPE and sPE patients. Both the level of total-miRNA and hsa-miR-210 expression was higher in case of severe PE. Conclusions: The level of circulating exosomal total-miRNA and hsa-miR-210 was elevated in women with PE, and it was higher in the severe form. We showed that hsa-miR-210 is secreted via exosomes, which may have a role in the pathomechanism of the disease. © 2017 International Society for the Study of Hypertension in Pregnancy. LA - English DB - MTMT ER - TY - JOUR AU - Mateescu, B AU - Kowal, EJ AU - van Balkom, BW AU - Bartel, S AU - Bhattacharyya, SN AU - Buzás, Edit Irén AU - Buck, AH AU - de Candia, P AU - Chow, FW AU - Das, S AU - Driedonks, TA AU - Fernandez-Messina, L AU - Haderk, F AU - Hill, AF AU - Jones, JC AU - Van, Keuren-Jensen KR AU - Lai, CP AU - Lasser, C AU - Liegro, ID AU - Lunavat, TR AU - Lorenowicz, MJ AU - Maas, SL AU - Mager, I AU - Mittelbrunn, M AU - Momma, S AU - Mukherjee, K AU - Nawaz, M AU - Pegtel, DM AU - Pfaffl, MW AU - Schiffelers, RM AU - Tahara, H AU - Thery, C AU - Tosar, JP AU - Wauben, MH AU - Witwer, KW AU - Nolte-'t, Hoen EN TI - Obstacles and opportunities in the functional analysis of extracellular vesicle RNA - an ISEV position paper. JF - JOURNAL OF EXTRACELLULAR VESICLES J2 - J EXTRACELLULAR VESICL VL - 6 PY - 2017 IS - 1 PG - 33 SN - 2001-3078 DO - 10.1080/20013078.2017.1286095 UR - https://m2.mtmt.hu/api/publication/3214053 ID - 3214053 N1 - Department of Biology, Swiss Federal Institute of Technology Zurich (ETH Zürich), Zurich, Switzerland Department of Biology, Massachusetts Institute of Technology, Cambridge, MA, United States Department of Nephrology and Hypertension, UMC Utrecht, Utrecht, Netherlands Experimental Asthma Research, Priority Area Asthma and Allergy, Research Center Borstel, Leibniz-Center for Medicine and Biosciences, Airway Research Center North (ARCN), Member of the German Center for Lung Research (DZL), Borstel, Germany Department of Science and Technology, CSIR-Indian Institute of Chemical Biology, Kolkata, India Department of Genetics, Cell- and Immunobiology, Semmelweis University, Budapest, Hungary Institute of Immunology and Infection Research, Centre for Immunity, Infection and Evolution, School of Biological Sciences, University of Edinburgh, Edinburgh, United Kingdom IRCCS MultiMedica, Milan, Italy Cardiovascular Research Institute, Massachusetts General Hospital, Boston, MA, United States Department of Biochemistry and Cell Biology, Faculty of Veterinary Medicine, Utrecht University, Utrecht, Netherlands Immunology Service, Hospital La Princesa, Madrid, Spain Department of Molecular Genetics, German Cancer Research Center (DKFZ), Heidelberg, Germany Department of Medicine, Helen Diller Family Comprehensive Cancer Center, UC San Francisco, San Francisco, CA, United States Department of Biochemistry and Genetics, La Trobe Institute for Molecular Science, La Trobe University, Bundoora, Australia Molecular Immunogenetics and Vaccine Research Section, Vaccine Branch, CCR, NCI, Bethesda, MD, United States Neurogenomics Division, TGen, Phoenix, AZ, United States Institute of Biomedical Engineering, National Tsing Hua University, Hsinchu, Taiwan Department of Neurology and Center for Molecular Imaging Research, Department of Radiology, Harvard Medical School, Massachusetts General Hospital and NeuroDiscovery Center, Boston, MA, United States Krefting Research Centre, Department of Internal Medicine and Clinical Nutrition, Sahlgrenska Academy, University of Gothenburg, Gothenburg, Sweden Department of Experimental Biomedicine and Clinical Neurosciences (BIONEC), University of Palermo, Palermo, Italy Center for Molecular Medicine, University Medical Center Utrecht and Regenerative Medicine Center, Utrecht, Netherlands Department of Physiology, Anatomy and Genetics, University of Oxford, Oxford, United Kingdom Institute of Technology, University of Tartu, Tartu, Estonia Instituto de Investigación del Hospital 12 de Octubre, Madrid, Spain Institute of Neurology (Edinger Institute), Frankfurt University Medical School, Frankfurt am Main, Germany Department of Pathology and Forensic Medicine, Ribeirão Preto School of Medicine, University of Sao Paulo, Sao Paulo, Brazil Department of Pathology, Exosomes Research Group, VU University Medical Center, Amsterdam, Netherlands Animal Physiology and Immunology, School of Life Sciences, Technical University of Munich (TUM) Weihenstephan, Freising, Germany Laboratory Clinical Chemistry and Haematology, University Medical Center Utrecht, Utrecht, Netherlands Department of Cellular and Molecular Biology, Institute of Biomedical and Health Sciences, Hiroshima University, Hiroshima, Japan Institut Curie, PSL Research University, INSERM U932, Paris, France Functional Genomics Unit, Institut Pasteur de Montevideo, Nuclear Research Center, Faculty of Science, Universidad de la República, Montevideo, Uruguay Department of Molecular and Comparative Pathobiology and Department of Neurology, The Johns Hopkins University School of Medicine, Baltimore, MD, United States Cited By :147 Export Date: 5 August 2019 Correspondence Address: Nolte-'t Hoen, E.N.M.; Department of Biochemistry and Cell Biology, Faculty of Veterinary Medicine, Utrecht UniversityNetherlands; email: e.n.m.nolte@uu.nl AB - The release of RNA-containing extracellular vesicles (EV) into the extracellular milieu has been demonstrated in a multitude of different in vitro cell systems and in a variety of body fluids. RNA-containing EV are in the limelight for their capacity to communicate genetically encoded messages to other cells, their suitability as candidate biomarkers for diseases, and their use as therapeutic agents. Although EV-RNA has attracted enormous interest from basic researchers, clinicians, and industry, we currently have limited knowledge on which mechanisms drive and regulate RNA incorporation into EV and on how RNA-encoded messages affect signalling processes in EV-targeted cells. Moreover, EV-RNA research faces various technical challenges, such as standardisation of EV isolation methods, optimisation of methodologies to isolate and characterise minute quantities of RNA found in EV, and development of approaches to demonstrate functional transfer of EV-RNA in vivo. These topics were discussed at the 2015 EV-RNA workshop of the International Society for Extracellular Vesicles. This position paper was written by the participants of the workshop not only to give an overview of the current state of knowledge in the field, but also to clarify that our incomplete knowledge - of the nature of EV(-RNA)s and of how to effectively and reliably study them - currently prohibits the implementation of gold standards in EV-RNA research. In addition, this paper creates awareness of possibilities and limitations of currently used strategies to investigate EV-RNA and calls for caution in interpretation of the obtained data. LA - English DB - MTMT ER - TY - JOUR AU - Van, Deun J AU - Mestdagh, P AU - Agostinis, P AU - Akay, O AU - Anand, S AU - Anckaert, J AU - Martinez, ZA AU - Baetens, T AU - Beghein, E AU - Bertier, L AU - Berx, G AU - Boere, J AU - Boukouris, S AU - Bremer, M AU - Buschmann, D AU - Byrd, JB AU - Casert, C AU - Cheng, L AU - Cmoch, A AU - Daveloose, D AU - De Smedt, E AU - Demirsoy, S AU - Depoorter, V AU - Dhondt, B AU - Driedonks, TA AU - Dudek, A AU - Elsharawy, A AU - Floris, I AU - Foers, AD AU - Gartner, K AU - Garg, AD AU - Geeurickx, E AU - Gettemans, J AU - Ghazavi, F AU - Giebel, B AU - Kormelink, TG AU - Hancock, G AU - Helsmoortel, H AU - Hill, AF AU - Hyenne, V AU - Kalra, H AU - Kim, D AU - Kowal, J AU - Kraemer, S AU - Leidinger, P AU - Leonelli, C AU - Liang, Y AU - Lippens, L AU - Liu, S AU - Lo, Cicero A AU - Martin, S AU - Mathivanan, S AU - Mathiyalagan, P AU - Matusek, T AU - Milani, G AU - Monguio-Tortajada, M AU - Mus, LM AU - Muth, DC AU - Németh, Andrea AU - Nolte-'t, Hoen EN AU - O'Driscoll, L AU - Palmulli, R AU - Pfaffl, MW AU - Primdal-Bengtson, B AU - Romano, E AU - Rousseau, Q AU - Sahoo, S AU - Sampaio, N AU - Samuel, M AU - Scicluna, B AU - Soen, B AU - Steels, A AU - Swinnen, JV AU - Takatalo, M AU - Thaminy, S AU - Thery, C AU - Tulkens, J AU - Van, Audenhove I AU - van der Grein, S AU - Van, Goethem A AU - van Herwijnen, MJ AU - Van, Niel G AU - Van, Roy N AU - Van, Vliet AR AU - Vandamme, N AU - Vanhauwaert, S AU - Vergauwen, G AU - Verweij, F AU - Wallaert, A AU - Wauben, M AU - Witwer, KW AU - Zonneveld, MI AU - De Wever, O AU - Vandesompele, J AU - Hendrix, A TI - EV-TRACK: transparent reporting and centralizing knowledge in extracellular vesicle research JF - NATURE METHODS J2 - NAT METHODS VL - 14 PY - 2017 IS - 3 SP - 228 EP - 232 PG - 5 SN - 1548-7091 DO - 10.1038/nmeth.4185 UR - https://m2.mtmt.hu/api/publication/3246966 ID - 3246966 N1 - EV-TRACK Consortium WoS:hiba:000395661700012 2019-03-14 06:44 első szerző nem egyezik Laboratory of Experimental Cancer Research, Department of Radiation Oncology and Experimental Cancer Research, Cancer Research Institute Ghent (CRIG), Ghent University, Ghent, Belgium Center for Medical Genetics, Cancer Research Institute Ghent (CRIG), Bioinformatics Institute Ghent (BIG), Ghent University, Ghent, Belgium Cell Death Research and Therapy (CDRT) Lab, KU Leuven University of Leuven, Leuven, Belgium Department of Biomedical Molecular Biology, Cancer Research Institute Ghent (CRIG), Ghent University, Ghent, Belgium Molecular and Cellular Oncology Lab, Inflammation Research Center, VIB, Ghent, Belgium Department of Biochemistry and Genetics, La Trobe Institute for Molecular Science, La Trobe University, Melbourne, Australia Unidad de Investigación, Hospital Santa Cristina Instituto de Investigación Sanitaria Princesa C, Madrid, Spain Department of Biochemistry, Faculty of Medicine and Health Sciences, Ghent University, Rommelaere Campus, Ghent, Belgium Department of Biochemistry and Cell Biology, Faculty of Veterinary Medicine, Utrecht University, Utrecht, Netherlands Institute for Transfusion Medicine, University Hospital Essen, University Duisburg-Essen, Essen, Germany Animal Physiology and Immunology, School of Life Sciences, Technical University of Munich (TUM) Weihenstephan, Freising, Germany Laboratory of Cytometry, Department of Internal Medicine, University of Michigan Medical School, University of Michigan, Ann Arbor, MI, United States Department of Biochemistry, Nencki Institute of Experimental Biology, Warsaw, Poland Institute of Clinical Molecular Biology, Christian-AlbrechtsUniversity of Kiel, Kiel, Germany Faculty of Sciences, Division of Biochemistry, Chemistry Department, Damietta University, New Damietta City, Egypt UMR-1280, INRA, University of Nantes, Physiologie des Adaptations Nutritionnelles, Nantes, France Department of Biochemistry, Microbiology and Immunology, University of Ottawa, Ottawa, ON, Canada Research Unit Gene Vectors, Helmholtz Zentrum München, Munich, Germany German Centre for Infection Research (DZIF), partner site Munich, Munich, Germany Department of Molecular and Comparative Pathobiology, Department of Neurology, Johns Hopkins University School of Medicine, Johns Hopkins University, Baltimore, MD, United States INSERM U1109, MN3T, Strasbourg, France LabEx Medalis, Université de Strasbourg, Strasbourg, France Fédération de Médecine Translationnelle de Strasbourg (FMTS), Strasbourg, France Cardiovascular Research Center, Icahn School of Medicine at Mount Sinai, New York, NY, United States Institut Curie, PSL Research University, INSERM U932, Paris, France Department of Thoracic and Cardiovascular Surgery, University Hospital RWTH Aachen, Germany Institute of Human Genetics, Saarland University, Homburg, Germany German Center for Neurodegenerative Diseases Bonn (DZNE E.V.), Bonn, Germany Institut Curie, PSL Research University, CNRS, UMR 144, Paris, France Université Côte d'Azur, Inserm, CNRS, IBV, Nice, France REMAR-IVECAT Group, Germans Trias i Pujol Health Science Research Institute, Can Ruti Campus, Autonomous University of Barcelona, Barcelona, Spain Department of Genetics, Cell-and Immunobiology, Semmelweis University, Budapest, Hungary School of Pharmacy and Pharmaceutical Sciences, Trinity Biomedical Sciences Institute, Trinity College Dublin, Dublin, Ireland Population Health and Immunity Division, Walter and Eliza Hall Institute of Medical Research, Parkville, VIC, Australia Laboratory of Lipid Metabolism and Cancer, Department of Oncology, LKI, Leuven Cancer Institute, KU Leuven, University of Leuven, Leuven, Belgium Biochemistry and Biotechnology, Faculty of Biological and Environmental Sciences, Division of Pharmaceutical Biosciences, Faculty of Pharmacy, University of Helsinki, Finland Division of Molecular and Cellular Medicine, National Cancer Center Research Institute, Tokyo, Japan Inflammation Division, Walter and Eliza Hall Institute of Medical Research, Parkville, VIC, Australia Department of Medical Biology, University of Melbourne, Parkville, VIC, Australia Cited By :150 Export Date: 5 August 2019 AB - We argue that the field of extracellular vesicle (EV) biology needs more transparent reporting to facilitate interpretation and replication of experiments. To achieve this, we describe EV-TRACK, a crowdsourcing knowledgebase (http://evtrack.org) that centralizes EV biology and methodology with the goal of stimulating authors, reviewers, editors and funders to put experimental guidelines into practice. LA - English DB - MTMT ER - TY - JOUR AU - Sódar, Barbara AU - Kittel, Ágnes AU - Pálóczi, Krisztina AU - Visnovitzné Dr Vukman, Krisztina AU - Osteikoetxea, Xabier AU - Szabó-Taylor, Katalin AU - Németh, Andrea AU - Sperlágh, Beáta AU - Baranyai, Tamás AU - Giricz, Zoltán AU - Wiener, Zoltán AU - Turiák, Lilla AU - Drahos, László AU - Pállinger, Éva AU - Vékey, Károly AU - Ferdinandy, Péter AU - Falus, András AU - Buzás, Edit Irén TI - Low-density lipoprotein mimics blood plasma-derived exosomes and microvesicles during isolation and detection JF - SCIENTIFIC REPORTS J2 - SCI REP VL - 6 PY - 2016 PG - 12 SN - 2045-2322 DO - 10.1038/srep24316 UR - https://m2.mtmt.hu/api/publication/3054080 ID - 3054080 N1 - Department of Genetics, Cell- and Immunobiology, Semmelweis University, Budapest, 1085, Hungary Institute of Experimental Medicine, Hungarian Academy of Sciences, Budapest, 1083, Hungary Department of Pharmacology and Pharmacotherapy, Semmelweis University, Budapest, 1085, Hungary Research Centre for Natural Sciences, Hungarian Academy of Sciences, Budapest, 1117, Hungary Cited By :254 Export Date: 18 May 2022 Correspondence Address: Buzás, E.I.; Department of Genetics, Hungary; email: edit.buzas@gmail.com Chemicals/CAS: Biomarkers; Lipoproteins, LDL Funding details: Seventh Framework Programme, FP7, 289033 Funding details: Semmelweis Egyetem Funding details: Hungarian Scientific Research Fund, OTKA, 104369, 109051, 112085, 11958, FP7-PEOPLE-2011-ITN-PITN-GA-2011-289033 Funding details: Magyar Tudományos Akadémia, MTA Funding text 1: The authors thank MA Werner for his continuous support and his excellent assistance with the experiments. They also thank N Fekete and A Orb?n for the technical support. This work was supported by National Scientific Research Program of Hungary (OTKA) #11958; #PD104369, #PD112085; #PD 109051, MEDINPROT Program, BMBS COST Action BM1202 ME HAD, FP7-PEOPLE-2011-ITN-PITN-GA-2011-289033 DYNANO, J?nos Bolyai Research Fellowship of the Hungarian Academy of Sciences (KVV, ZG and ZW), Semmelweis University Start up Grant (ZW) and the Kerpel Fronius Program of Semmelweis University (Astellas Pharma Grant). AB - Circulating extracellular vesicles have emerged as potential new biomarkers in a wide variety of diseases. Despite the increasing interest, their isolation and purification from body fluids remains challenging. Here we studied human pre-prandial and 4 hours postprandial platelet-free blood plasma samples as well as human platelet concentrates. Using flow cytometry, we found that the majority of circulating particles within the size range of extracellular vesicles lacked common vesicular markers. We identified most of these particles as lipoproteins (predominantly low-density lipoprotein, LDL) which mimicked the characteristics of extracellular vesicles and also co-purified with them. Based on biophysical properties of LDL this finding was highly unexpected. Current state-of-the-art extracellular vesicle isolation and purification methods did not result in lipoprotein-free vesicle preparations from blood plasma or from platelet concentrates. Furthermore, transmission electron microscopy showed an association of LDL with isolated vesicles upon in vitro mixing. This is the first study to show co-purification and in vitro association of LDL with extracellular vesicles and its interference with vesicle analysis. Our data point to the importance of careful study design and data interpretation in studies using blood-derived extracellular vesicles with special focus on potentially co-purified LDL. LA - English DB - MTMT ER - TY - JOUR AU - Yanez-Mo, M AU - Siljander, PR AU - Andreu, Z AU - Zavec, AB AU - Borras, FE AU - Buzás, Edit Irén AU - Buzás, Krisztina AU - Casal, E AU - Cappello, F AU - Carvalho, J AU - Colas, E AU - Cordeiro-da Silva, A AU - Fais, S AU - Falcon-Perez, JM AU - Ghobrial, IM AU - Giebel, B AU - Gimona, M AU - Graner, M AU - Gursel, I AU - Gursel, M AU - Heegaard, NH AU - Hendrix, A AU - Kierulf, P AU - Kokubun, K AU - Kosanovic, M AU - Kralj-Iglic, V AU - Kramer-Albers, EM AU - Laitinen, S AU - Lasser, C AU - Lener, T AU - Ligeti, Erzsébet AU - Line, A AU - Lipps, G AU - Llorente, A AU - Lotvall, J AU - Mancek-Keber, M AU - Marcilla, A AU - Mittelbrunn, M AU - Nazarenko, I AU - Nolte-'t, Hoen EN AU - Nyman, TA AU - O'Driscoll, L AU - Olivan, M AU - Oliveira, C AU - Pállinger, Éva AU - Del Portillo, HA AU - Reventos, J AU - Rigau, M AU - Rohde, E AU - Sammar, M AU - Sanchez-Madrid, F AU - Santarem, N AU - Schallmoser, K AU - Ostenfeld, MS AU - Stoorvogel, W AU - Stukelj, R AU - Van, der Grein SG AU - Vasconcelos, MH AU - Wauben, MH AU - De Wever, O TI - Biological properties of extracellular vesicles and their physiological functions JF - JOURNAL OF EXTRACELLULAR VESICLES J2 - J EXTRACELLULAR VESICL VL - 4 PY - 2015 PG - 60 SN - 2001-3078 DO - 10.3402/jev.v4.27066 UR - https://m2.mtmt.hu/api/publication/2930099 ID - 2930099 N1 - Yanez-Mo M and Siljander PR authors have contributed equally. The rest of the authors are listed in alphabetical order. Unidad de Investigación, Hospital Sta Cristina, Instituto de Investigaciones Sanitarias Princesa (IIS-IP), Madrid, Spain Departamento de Biología Molecular, UAM, Madrid, Spain Extracellular Vesicle Research, Division of Biochemistry and Biotechnology, Department of Biosciences, University of Helsinki, Helsinki, Finland Division of Pharmaceutical Biosciences, Faculty of Pharmacy, University of Helsinki, Helsinki, Finland Laboratory for Molecular Biology and Nanobiotechnology, National Institute of Chemistry, Ljubljana, Slovenia IVECAT Group, 'Germans Trias i Pujol' Research Institute, Badalona, Spain Nephrology Service, 'Germans Trias i Pujol' University Hospital, Badalona, Spain Department of Genetics, Cell- and Immunobiology, Semmelweis University, Budapest, Hungary Biological Research Centre, Hungarian Academy of Sciences, Szeged, Hungary Faculty of Dentistry, University of Szeged, Szeged, Hungary Metabolomics Unit, CIC bioGUNE, CIBERehd, Bizkaia Technology Park, Derio, Spain Department of Experimental Biomedicine and Clinical Neuroscience, Human Anatomy Section, University of Palermo, Palermo, Italy Euro-Mediterranean Institute of Science and Technology, Palermo, Italy Instituto de Investigação e Inovação em Saú de, Universidade do Porto, Porto, Portugal Expression Regulation in Cancer, Institute of Molecular Pathology and Immunology of the University of Porto (IPATIMUP), Porto, Portugal Research Unit in Biomedicine and Translational Oncology, Vall Hebron Institute of Research and Autonomous, University of Barcelona, Barcelona, Spain IBMC, Instituto de Biologia Molecular e Celular, Universidade do Porto, Porto, Portugal Department of Biological Sciences, Faculty of Pharmacy, University of Porto, Porto, Portugal Anti-Tumour Drugs Section, Department of Therapeutic Research and Medicines Evaluation, National Institute of Health (ISS), Rome, Italy IKERBASQUE, Basque Foundation for Science, Bilbao, Spain Dana-Farber Cancer Institute, Boston, MA, United States Institute for Transfusion Medicine, University Hospital Essen, University of Duisburg-Essen, Essen, Germany Spinal Cord Injury and Tissue Regeneration Center Salzburg (SCI-TReCS), Paracelsus Medical University (PMU), Salzburg, Austria Department of Blood Group Serology and Transfusion Medicine, Universitätsklinikum, Salzburger Landeskliniken GesmbH (SALK), Salzburg, Austria Department of Neurosurgery, University of Colorado DenverCO, United States Department of Molecular Biology and Genetics, Thorlab-Therapeutic Oligonucleotide Research Lab, Bilkent University, Ankara, Turkey Department of Biological Sciences, Middle East Technical University, Ankara, Turkey Department of Clinical Biochemistry and Pharmacology, Odense University Hospital, University of Southern Denmark, Odense, Denmark Analytical Protein Chemistry, Department of Clinical Biochemistry, Immunology and Genetics, Statens Serum Institut, Copenhagen, Denmark Laboratory of Experimental Cancer Research, Department of Radiation Oncology and Experimental Cancer Research, Ghent University Hospital, Ghent, Belgium Bood Cell Research Group, Department of Medical Biochemistry, Oslo University Hospital, Oslo, Norway Department of Immunochemistry and Glycobiology, Institute for the Application of Nuclear Energy, INEP, University of Belgrade, Belgrade, Serbia Laboratory of Clinical Biophysics, Faculty of Health Sciences, University of Ljubljana, Ljubljana, Slovenia Molecular Cell Biology and Focus Program Translational Neurosciences, University of Mainz, Mainz, Germany Research and Cell Services, Finnish Red Cross Blood Service, Helsinki, Finland Krefting Research Centre, Institute of Medicine at the Sahlgrenska Academy, University of Gothenburg, Gothenburg, Sweden Department of Physiology, Semmelweis University, Budapest, Hungary Latvian Biomedical Research and Study Centre, Riga, Latvia Institute of Chemistry and Bioanalytics, School of Life Sciences, University of Applied Sciences and Arts Northwestern Switzerland, Muttenz, Switzerland Department of Molecular Cell Biology, Institute for Cancer Research, Oslo University Hospital - The Norwegian Radium Hospital, Oslo, Norway National Institute of Chemistry, Laboratory of Biotechnology, Ljubljana, Slovenia ENFIST Centre of Excellence, Ljubljana, Slovenia Departamento de Biología Celular y Parasitologia, Facultat de Farmacia, Universitat de Valencia, Valencia, Spain Department of Vascular Biology and Inflammation, Centro Nacional de Investigaciones Cardiovasculares, Madrid, Spain Institute for Environmental Health Sciences, Hospital Infection Control Medical Center, University of Freiburg, Freiburg im Breisgau, Germany Department of Biochemistry and Cell Biology, Faculty of Veterinary Medicine, Utrecht University, Utrecht, Netherlands Institute of Biotechnology, University of Helsinki, Viikinkaari 1, Helsinki, Finland School of Pharmacy and Pharmaceutical Sciences, Trinity Biomedical Sciences Institute, Trinity College Dublin, Dublin, Ireland Department of Pathology and Oncology, Faculty of Medicine, University of Porto, Porto, Portugal ISGlobal, Barcelona Ctr. Int. Health Res. (CRESIB), Hospital Clínic - Universitat de Barcelona, Barcelona, Spain Institució Catalana de Recerca I Estudis Avançats, Barcelona, Spain Departament de Ciències Bàsiques, Universitat Internacional de Catalunya, Institut de Recerca Biomèdica de Bellvitge, Barcelona, Spain Department of Biotechnology Engineering, ORT Braude College, Karmiel, Israel Servicio de Inmunología, Hospital de la Princesa, Instituto de Investigaciones Sanitarias Princesa (IIS-IP), Madrid, Spain Departmnet of Molecular Medicine, Aarhus University Hospital, Aarhus, Denmark Cancer Drug Resistance Group, Institute of Molecular Pathology and Immunology, University of Porto (IPATIMUP), Porto, Portugal Department of Biological Sciences, Faculty of Pharmacy, University of Porto (FFUP), Porto, Portugal Cited By :955 Export Date: 30 August 2019 Correspondence Address: Yáñez-Mó, M.; Membrane Microdomains in Immunity Laboratory, Unidad de Investigación, Hospital Santa Cristina, Instituto de Investigación Sanitaria Princesa, Departamento de Biología Molecular, UAM, C/Maestro Amadeo Vives 2, edificio consultas 5a planta, Spain Chemicals/CAS: DNA, 9007-49-2; lipid, 66455-18-3; RNA, 63231-63-0 Unidad de Investigación, Hospital Sta Cristina, Instituto de Investigaciones Sanitarias Princesa (IIS-IP), Madrid, Spain Departamento de Biología Molecular, UAM, Madrid, Spain Extracellular Vesicle Research, Division of Biochemistry and Biotechnology, Department of Biosciences, University of Helsinki, Helsinki, Finland Division of Pharmaceutical Biosciences, Faculty of Pharmacy, University of Helsinki, Helsinki, Finland Laboratory for Molecular Biology and Nanobiotechnology, National Institute of Chemistry, Ljubljana, Slovenia IVECAT Group, 'Germans Trias i Pujol' Research Institute, Badalona, Spain Nephrology Service, 'Germans Trias i Pujol' University Hospital, Badalona, Spain Department of Genetics, Cell- and Immunobiology, Semmelweis University, Budapest, Hungary Biological Research Centre, Hungarian Academy of Sciences, Szeged, Hungary Faculty of Dentistry, University of Szeged, Szeged, Hungary Metabolomics Unit, CIC bioGUNE, CIBERehd, Bizkaia Technology Park, Derio, Spain Department of Experimental Biomedicine and Clinical Neuroscience, Human Anatomy Section, University of Palermo, Palermo, Italy Euro-Mediterranean Institute of Science and Technology, Palermo, Italy Instituto de Investigação e Inovação em Saú de, Universidade do Porto, Porto, Portugal Expression Regulation in Cancer, Institute of Molecular Pathology and Immunology of the University of Porto (IPATIMUP), Porto, Portugal Research Unit in Biomedicine and Translational Oncology, Vall Hebron Institute of Research and Autonomous, University of Barcelona, Barcelona, Spain IBMC, Instituto de Biologia Molecular e Celular, Universidade do Porto, Porto, Portugal Department of Biological Sciences, Faculty of Pharmacy, University of Porto, Porto, Portugal Anti-Tumour Drugs Section, Department of Therapeutic Research and Medicines Evaluation, National Institute of Health (ISS), Rome, Italy IKERBASQUE, Basque Foundation for Science, Bilbao, Spain Dana-Farber Cancer Institute, Boston, MA, United States Institute for Transfusion Medicine, University Hospital Essen, University of Duisburg-Essen, Essen, Germany Spinal Cord Injury and Tissue Regeneration Center Salzburg (SCI-TReCS), Paracelsus Medical University (PMU), Salzburg, Austria Department of Blood Group Serology and Transfusion Medicine, Universitätsklinikum, Salzburger Landeskliniken GesmbH (SALK), Salzburg, Austria Department of Neurosurgery, University of Colorado DenverCO, United States Department of Molecular Biology and Genetics, Thorlab-Therapeutic Oligonucleotide Research Lab, Bilkent University, Ankara, Turkey Department of Biological Sciences, Middle East Technical University, Ankara, Turkey Department of Clinical Biochemistry and Pharmacology, Odense University Hospital, University of Southern Denmark, Odense, Denmark Analytical Protein Chemistry, Department of Clinical Biochemistry, Immunology and Genetics, Statens Serum Institut, Copenhagen, Denmark Laboratory of Experimental Cancer Research, Department of Radiation Oncology and Experimental Cancer Research, Ghent University Hospital, Ghent, Belgium Bood Cell Research Group, Department of Medical Biochemistry, Oslo University Hospital, Oslo, Norway Department of Immunochemistry and Glycobiology, Institute for the Application of Nuclear Energy, INEP, University of Belgrade, Belgrade, Serbia Laboratory of Clinical Biophysics, Faculty of Health Sciences, University of Ljubljana, Ljubljana, Slovenia Molecular Cell Biology and Focus Program Translational Neurosciences, University of Mainz, Mainz, Germany Research and Cell Services, Finnish Red Cross Blood Service, Helsinki, Finland Krefting Research Centre, Institute of Medicine at the Sahlgrenska Academy, University of Gothenburg, Gothenburg, Sweden Department of Physiology, Semmelweis University, Budapest, Hungary Latvian Biomedical Research and Study Centre, Riga, Latvia Institute of Chemistry and Bioanalytics, School of Life Sciences, University of Applied Sciences and Arts Northwestern Switzerland, Muttenz, Switzerland Department of Molecular Cell Biology, Institute for Cancer Research, Oslo University Hospital - The Norwegian Radium Hospital, Oslo, Norway National Institute of Chemistry, Laboratory of Biotechnology, Ljubljana, Slovenia ENFIST Centre of Excellence, Ljubljana, Slovenia Departamento de Biología Celular y Parasitologia, Facultat de Farmacia, Universitat de Valencia, Valencia, Spain Department of Vascular Biology and Inflammation, Centro Nacional de Investigaciones Cardiovasculares, Madrid, Spain Institute for Environmental Health Sciences, Hospital Infection Control Medical Center, University of Freiburg, Freiburg im Breisgau, Germany Department of Biochemistry and Cell Biology, Faculty of Veterinary Medicine, Utrecht University, Utrecht, Netherlands Institute of Biotechnology, University of Helsinki, Viikinkaari 1, Helsinki, Finland School of Pharmacy and Pharmaceutical Sciences, Trinity Biomedical Sciences Institute, Trinity College Dublin, Dublin, Ireland Department of Pathology and Oncology, Faculty of Medicine, University of Porto, Porto, Portugal ISGlobal, Barcelona Ctr. Int. Health Res. (CRESIB), Hospital Clínic - Universitat de Barcelona, Barcelona, Spain Institució Catalana de Recerca I Estudis Avançats, Barcelona, Spain Departament de Ciències Bàsiques, Universitat Internacional de Catalunya, Institut de Recerca Biomèdica de Bellvitge, Barcelona, Spain Department of Biotechnology Engineering, ORT Braude College, Karmiel, Israel Servicio de Inmunología, Hospital de la Princesa, Instituto de Investigaciones Sanitarias Princesa (IIS-IP), Madrid, Spain Departmnet of Molecular Medicine, Aarhus University Hospital, Aarhus, Denmark Cancer Drug Resistance Group, Institute of Molecular Pathology and Immunology, University of Porto (IPATIMUP), Porto, Portugal Department of Biological Sciences, Faculty of Pharmacy, University of Porto (FFUP), Porto, Portugal Cited By :1100 Export Date: 14 January 2020 Correspondence Address: Yáñez-Mó, M.; Membrane Microdomains in Immunity Laboratory, Unidad de Investigación, Hospital Santa Cristina, Instituto de Investigación Sanitaria Princesa, Departamento de Biología Molecular, UAM, C/Maestro Amadeo Vives 2, edificio consultas 5a planta, Spain Chemicals/CAS: DNA, 9007-49-2; lipid, 66455-18-3; RNA, 63231-63-0 Funding Agency and Grant Number: European Network on Microvesicles and Exosomes in Health & Disease (ME-HaD) [BM1202]; ICREAICREA Funding text: The authors wish to thank Dr R Simpson and Dr D Taylor for critical reading of the manuscript and acknowledge the Horizon 2020 European Cooperation in Science and Technology programme and its support of our European Network on Microvesicles and Exosomes in Health & Disease (ME-HaD; BM1202 www.cost.eu/COST_Actions/bmbs/Actions/BM1202) Unidad de Investigación, Hospital Sta Cristina, Instituto de Investigaciones Sanitarias Princesa (IIS-IP), Madrid, Spain Departamento de Biología Molecular, UAM, Madrid, Spain Extracellular Vesicle Research, Division of Biochemistry and Biotechnology, Department of Biosciences, University of Helsinki, Helsinki, Finland Division of Pharmaceutical Biosciences, Faculty of Pharmacy, University of Helsinki, Helsinki, Finland Laboratory for Molecular Biology and Nanobiotechnology, National Institute of Chemistry, Ljubljana, Slovenia IVECAT Group, 'Germans Trias i Pujol' Research Institute, Badalona, Spain Nephrology Service, 'Germans Trias i Pujol' University Hospital, Badalona, Spain Department of Genetics, Cell- and Immunobiology, Semmelweis University, Budapest, Hungary Biological Research Centre, Hungarian Academy of Sciences, Szeged, Hungary Faculty of Dentistry, University of Szeged, Szeged, Hungary Metabolomics Unit, CIC bioGUNE, CIBERehd, Bizkaia Technology Park, Derio, Spain Department of Experimental Biomedicine and Clinical Neuroscience, Human Anatomy Section, University of Palermo, Palermo, Italy Euro-Mediterranean Institute of Science and Technology, Palermo, Italy Instituto de Investigação e Inovação em Saú de, Universidade do Porto, Porto, Portugal Expression Regulation in Cancer, Institute of Molecular Pathology and Immunology of the University of Porto (IPATIMUP), Porto, Portugal Research Unit in Biomedicine and Translational Oncology, Vall Hebron Institute of Research and Autonomous, University of Barcelona, Barcelona, Spain IBMC, Instituto de Biologia Molecular e Celular, Universidade do Porto, Porto, Portugal Department of Biological Sciences, Faculty of Pharmacy, University of Porto, Porto, Portugal Anti-Tumour Drugs Section, Department of Therapeutic Research and Medicines Evaluation, National Institute of Health (ISS), Rome, Italy IKERBASQUE, Basque Foundation for Science, Bilbao, Spain Dana-Farber Cancer Institute, Boston, MA, United States Institute for Transfusion Medicine, University Hospital Essen, University of Duisburg-Essen, Essen, Germany Spinal Cord Injury and Tissue Regeneration Center Salzburg (SCI-TReCS), Paracelsus Medical University (PMU), Salzburg, Austria Department of Blood Group Serology and Transfusion Medicine, Universitätsklinikum, Salzburger Landeskliniken GesmbH (SALK), Salzburg, Austria Department of Neurosurgery, University of Colorado DenverCO, United States Department of Molecular Biology and Genetics, Thorlab-Therapeutic Oligonucleotide Research Lab, Bilkent University, Ankara, Turkey Department of Biological Sciences, Middle East Technical University, Ankara, Turkey Department of Clinical Biochemistry and Pharmacology, Odense University Hospital, University of Southern Denmark, Odense, Denmark Analytical Protein Chemistry, Department of Clinical Biochemistry, Immunology and Genetics, Statens Serum Institut, Copenhagen, Denmark Laboratory of Experimental Cancer Research, Department of Radiation Oncology and Experimental Cancer Research, Ghent University Hospital, Ghent, Belgium Bood Cell Research Group, Department of Medical Biochemistry, Oslo University Hospital, Oslo, Norway Department of Immunochemistry and Glycobiology, Institute for the Application of Nuclear Energy, INEP, University of Belgrade, Belgrade, Serbia Laboratory of Clinical Biophysics, Faculty of Health Sciences, University of Ljubljana, Ljubljana, Slovenia Molecular Cell Biology and Focus Program Translational Neurosciences, University of Mainz, Mainz, Germany Research and Cell Services, Finnish Red Cross Blood Service, Helsinki, Finland Krefting Research Centre, Institute of Medicine at the Sahlgrenska Academy, University of Gothenburg, Gothenburg, Sweden Department of Physiology, Semmelweis University, Budapest, Hungary Latvian Biomedical Research and Study Centre, Riga, Latvia Institute of Chemistry and Bioanalytics, School of Life Sciences, University of Applied Sciences and Arts Northwestern Switzerland, Muttenz, Switzerland Department of Molecular Cell Biology, Institute for Cancer Research, Oslo University Hospital - The Norwegian Radium Hospital, Oslo, Norway National Institute of Chemistry, Laboratory of Biotechnology, Ljubljana, Slovenia ENFIST Centre of Excellence, Ljubljana, Slovenia Departamento de Biología Celular y Parasitologia, Facultat de Farmacia, Universitat de Valencia, Valencia, Spain Department of Vascular Biology and Inflammation, Centro Nacional de Investigaciones Cardiovasculares, Madrid, Spain Institute for Environmental Health Sciences, Hospital Infection Control Medical Center, University of Freiburg, Freiburg im Breisgau, Germany Department of Biochemistry and Cell Biology, Faculty of Veterinary Medicine, Utrecht University, Utrecht, Netherlands Institute of Biotechnology, University of Helsinki, Viikinkaari 1, Helsinki, Finland School of Pharmacy and Pharmaceutical Sciences, Trinity Biomedical Sciences Institute, Trinity College Dublin, Dublin, Ireland Department of Pathology and Oncology, Faculty of Medicine, University of Porto, Porto, Portugal ISGlobal, Barcelona Ctr. Int. Health Res. (CRESIB), Hospital Clínic - Universitat de Barcelona, Barcelona, Spain Institució Catalana de Recerca I Estudis Avançats, Barcelona, Spain Departament de Ciències Bàsiques, Universitat Internacional de Catalunya, Institut de Recerca Biomèdica de Bellvitge, Barcelona, Spain Department of Biotechnology Engineering, ORT Braude College, Karmiel, Israel Servicio de Inmunología, Hospital de la Princesa, Instituto de Investigaciones Sanitarias Princesa (IIS-IP), Madrid, Spain Departmnet of Molecular Medicine, Aarhus University Hospital, Aarhus, Denmark Cancer Drug Resistance Group, Institute of Molecular Pathology and Immunology, University of Porto (IPATIMUP), Porto, Portugal Department of Biological Sciences, Faculty of Pharmacy, University of Porto (FFUP), Porto, Portugal Cited By :1581 Export Date: 13 January 2021 Correspondence Address: Yáñez-Mó, M.; Membrane Microdomains in Immunity Laboratory, Unidad de Investigación, Hospital Santa Cristina, Instituto de Investigación Sanitaria Princesa, Departamento de Biología Molecular, UAM, C/Maestro Amadeo Vives 2, edificio consultas 5a planta, Spain Chemicals/CAS: DNA, 9007-49-2; lipid, 66455-18-3; RNA, 63231-63-0 Funding Agency and Grant Number: European Network on Microvesicles and Exosomes in Health & Disease (ME-HaD) [BM1202]; ICREAICREA Funding Source: Custom Funding text: The authors wish to thank Dr R Simpson and Dr D Taylor for critical reading of the manuscript and acknowledge the Horizon 2020 European Cooperation in Science and Technology programme and its support of our European Network on Microvesicles and Exosomes in Health & Disease (ME-HaD; BM1202 www.cost.eu/COST_Actions/bmbs/Actions/BM1202) AB - In the past decade, extracellular vesicles (EVs) have been recognized as potent vehicles of intercellular communication, both in prokaryotes and eukaryotes. This is due to their capacity to transfer proteins, lipids and nucleic acids, thereby influencing various physiological and pathological functions of both recipient and parent cells. While intensive investigation has targeted the role of EVs in different pathological processes, for example, in cancer and autoimmune diseases, the EV-mediated maintenance of homeostasis and the regulation of physiological functions have remained less explored. Here, we provide a comprehensive overview of the current understanding of the physiological roles of EVs, which has been written by crowd-sourcing, drawing on the unique EV expertise of academia-based scientists, clinicians and industry based in 27 European countries, the United States and Australia. This review is intended to be of relevance to both researchers already working on EV biology and to newcomers who will encounter this universal cell biological system. Therefore, here we address the molecular contents and functions of EVs in various tissues and body fluids from cell systems to organs. We also review the physiological mechanisms of EVs in bacteria, lower eukaryotes and plants to highlight the functional uniformity of this emerging communication system. LA - English DB - MTMT ER -