@article{MTMT:3273020,
	title = {Various levels of circulating exosomal total-miRNA and miR-210 hypoxamiR in different forms of pregnancy hypertension},
	url = {https://m2.mtmt.hu/api/publication/3273020},
	author = {Biró, Orsolya and Alasztics, Bálint and Molvarec, Attila and Joó, József Gábor and Nagy, Bálint and Rigó, János},
	doi = {10.1016/j.preghy.2017.09.002},
	journal-iso = {PREGNANCY HYPERTENS},
	journal = {PREGNANCY HYPERTENSION},
	volume = {10},
	unique-id = {3273020},
	issn = {2210-7789},
	abstract = {Introduction: Hypertension is a common complication during pregnancy, affecting 10% of pregnant women worldwide. Several microRNA (miRNA) were shown to be involved in hypertensive disorders of pregnancy. In preeclampsia (PE), placental dysfunction causes the enhanced release of extracellular vesicle-derived miRNAs. The hypoxia-sensitive hsa-mir-210 is the most common PE-associated miRNA, but its exosomal profile has not been investigated. Objectives: Our aims were to measure exosomal total-miRNA concentration and to perform expression analysis of circulating exosomal hsa-miR-210 in women affected by chronic hypertension (CHT) gestational hypertension (GHT) or PE. Materials and methods: We collected plasma samples from women with CHT, GHT, PE (moderate: mPE and severe: sPE) and from normotensive pregnancies. Exosomal miRNAs were extracted and miRNA concentration was measured. RT-PCR was carried out with hsa-miR-210-3p-specific primers and relative expression was calculated using the comparative Ct method. Results: The total-miRNA concentration was different in the disease subgroups, and was significantly higher in mPE and sPE compared to the other groups. We found a significant difference in the relative exosomal hsa-miR-210-3p expression between all hypertensive groups compared to the normotensive samples, but significant upregulation was only observed in case of mPE and sPE patients. Both the level of total-miRNA and hsa-miR-210 expression was higher in case of severe PE. Conclusions: The level of circulating exosomal total-miRNA and hsa-miR-210 was elevated in women with PE, and it was higher in the severe form. We showed that hsa-miR-210 is secreted via exosomes, which may have a role in the pathomechanism of the disease. © 2017 International Society for the Study of Hypertension in Pregnancy.},
	keywords = {PREECLAMPSIA; microRNA; exosome; Maternal circulation},
	year = {2017},
	eissn = {2210-7797},
	pages = {207-212},
	orcid-numbers = {Biró, Orsolya/0000-0002-4300-3602; Alasztics, Bálint/0000-0002-4011-8439; Molvarec, Attila/0000-0002-3229-3034; Joó, József Gábor/0000-0001-9820-6514; Nagy, Bálint/0000-0002-0295-185X; Rigó, János/0000-0003-2762-6516}
}