TY - JOUR AU - Donahue, Mary J. AU - Kaszás, Attila AU - Turi, Gergely F. AU - Rózsa J., Balázs AU - Slézia, Andrea AU - Vanzetta, Ivo AU - Katona, Gergely AU - Bernard, Christophe AU - Malliaras, George G. AU - Williamson, Adam (John) TI - Multimodal Characterization of Neural Networks using Highly Transparent Electrode Arrays JF - ENEURO J2 - ENEURO VL - 5 PY - 2018 IS - 6 SN - 2373-2822 DO - 10.1523/ENEURO.0187-18.2018 UR - https://m2.mtmt.hu/api/publication/30382220 ID - 30382220 N1 - Department of Bioelectronics, Ecole Nationale Supérieure des Mines, Centre of Microelectronics in Provence, Gardanne, 13541, France Aix Marseille Univ, CNRS, INT, Inst Neurosci Timone, Marseille, France Department of Psychiatry, Division of Systems Neuroscience, Columbia University and Research Foundation for Mental Hygiene, New York State Psychiatric Institute, New York, NY 10032, United States Laboratory of 3D Functional Network and Dendritic Imaging, Institute of Experimental Medicine, Hungarian Academy of Sciences, Budapest, H-1083, Hungary Aix Marseille Univ, INSERM, INS, Inst Neurosci Syst, Marseille, France Neuroengineering Research Group, Interdisciplinary Excellence Center, Department of Medical Microbiology and Immunobiology, University of Szeged, Szeged, 6720, Hungary Two-Photon Measurement Technology Research Group, Pázmány Péter Catholic University, Budapest, H-1083, Hungary Electrical Engineering Division, Department of Engineering, University of Cambridge, Cambridge, CB3 0FA, United Kingdom Export Date: 30 July 2019 Correspondence Address: Williamson, A.; Aix- Marseille Université, Inserm U1106 INS, Institut de Neurosciences des SystèmesFrance; email: adam.williamson@univ-amu.fr Funding details: University of Cambridge Funding details: Ecole Supérieure des Communications de Tunis Funding details: Institut National de la Santé et de la Recherche Médicale, Inserm Funding details: Centre National de la Recherche Scientifique, CNRS Funding details: Marie Curie, 625372 Funding details: European Research Council, ERC Funding details: Fondation pour la Recherche Médicale, FRM, DBS20131128446, KFI-2016-0177, GINOP-2016-00979, NVKP-2016-0043, ERC682426 Funding details: 716867 Funding text 1: 1Department of Bioelectronics, Ecole Nationale Supérieure des Mines, Centre of Microelectronics in Provence, Gardanne 13541, France, 2Aix Marseille Univ, CNRS, INT, Inst Neurosci Timone, Marseille, France, 3Department of Psychiatry, Division of Systems Neuroscience, Columbia University and Research Foundation for Mental Hygiene, New York State Psychiatric Institute, New York, NY 10032, 4Laboratory of 3D Functional Network and Dendritic Imaging, Institute of Experimental Medicine, Hungarian Academy of Sciences, Budapest H-1083, Hungary, 5Aix Marseille Univ, INSERM, INS, Inst Neurosci Syst, Marseille, France, 6Neuroengineering Research Group, Interdisciplinary Excellence Center, Department of Medical Microbiology and Immunobiology, University of Szeged, Szeged 6720, Hungary, 7Two-Photon Measurement Technology Research Group, Pázmány Péter Catholic University, Budapest H-1083, Hungary, and 8Electrical Engineering Division, Department of Engineering, University of Cambridge, Cambridge CB3 0FA, United Kingdom Funding text 2: This work was supported by the European Research Council (ERC) under the European Union’s Horizon 2020 Research and Innovation Programme (Grant 716867). A.K. was supported by EC Marie Curie Intra-European Fellowship (ImagINE, Grant 625372). M.J.D. was supported by the Fondation pour la Recherche Médicale Grant DBS20131128446. B.R. and K.G. were supported by ERC682426, KFI-2016-0177, GINOP-2016-00979, and NVKP-2016-0043. *M.J.D. and A.K. contributed equally to this work. Department of Bioelectronics, Ecole Nationale Supérieure des Mines, Centre of Microelectronics in Provence, Gardanne, 13541, France Aix Marseille Univ, CNRS, INT, Inst Neurosci Timone, Marseille, France Department of Psychiatry, Division of Systems Neuroscience, Columbia University and Research Foundation for Mental Hygiene, New York State Psychiatric Institute, New York, NY 10032, United States Laboratory of 3D Functional Network and Dendritic Imaging, Institute of Experimental Medicine, Hungarian Academy of Sciences, Budapest, H-1083, Hungary Aix Marseille Univ, INSERM, INS, Inst Neurosci Syst, Marseille, France Neuroengineering Research Group, Interdisciplinary Excellence Center, Department of Medical Microbiology and Immunobiology, University of Szeged, Szeged, 6720, Hungary Two-Photon Measurement Technology Research Group, Pázmány Péter Catholic University, Budapest, H-1083, Hungary Electrical Engineering Division, Department of Engineering, University of Cambridge, Cambridge, CB3 0FA, United Kingdom Export Date: 15 February 2020 Correspondence Address: Williamson, A.; Aix- Marseille Université, Inserm U1106 INS, Institut de Neurosciences des SystèmesFrance; email: adam.williamson@univ-amu.fr Chemicals/CAS: calcium, 7440-70-2, 14092-94-5; fampridine, 1003-40-3, 504-24-5 Tradenames: Clevios, Heraeus, Germany Manufacturers: Femtonics; Heraeus, Germany; Scientifica Funding details: University of Cambridge Funding details: Ecole Supérieure des Communications de Tunis Funding details: Institut National de la Santé et de la Recherche Médicale, Inserm Funding details: Centre National de la Recherche Scientifique, CNRS Funding details: Marie Curie, 625372 Funding details: European Research Council, ERC Funding details: Fondation pour la Recherche Médicale, FRM, DBS20131128446, KFI-2016-0177, GINOP-2016-00979, NVKP-2016-0043, ERC682426 Funding details: 716867 Funding text 1: 1Department of Bioelectronics, Ecole Nationale Supérieure des Mines, Centre of Microelectronics in Provence, Gardanne 13541, France, 2Aix Marseille Univ, CNRS, INT, Inst Neurosci Timone, Marseille, France, 3Department of Psychiatry, Division of Systems Neuroscience, Columbia University and Research Foundation for Mental Hygiene, New York State Psychiatric Institute, New York, NY 10032, 4Laboratory of 3D Functional Network and Dendritic Imaging, Institute of Experimental Medicine, Hungarian Academy of Sciences, Budapest H-1083, Hungary, 5Aix Marseille Univ, INSERM, INS, Inst Neurosci Syst, Marseille, France, 6Neuroengineering Research Group, Interdisciplinary Excellence Center, Department of Medical Microbiology and Immunobiology, University of Szeged, Szeged 6720, Hungary, 7Two-Photon Measurement Technology Research Group, Pázmány Péter Catholic University, Budapest H-1083, Hungary, and 8Electrical Engineering Division, Department of Engineering, University of Cambridge, Cambridge CB3 0FA, United Kingdom Funding text 2: This work was supported by the European Research Council (ERC) under the European Union’s Horizon 2020 Research and Innovation Programme (Grant 716867). A.K. was supported by EC Marie Curie Intra-European Fellowship (ImagINE, Grant 625372). M.J.D. was supported by the Fondation pour la Recherche Médicale Grant DBS20131128446. B.R. and K.G. were supported by ERC682426, KFI-2016-0177, GINOP-2016-00979, and NVKP-2016-0043. *M.J.D. and A.K. contributed equally to this work. Department of Bioelectronics, Ecole Nationale Supérieure des Mines, Centre of Microelectronics in Provence, Gardanne, 13541, France Aix Marseille Univ, CNRS, INT, Inst Neurosci Timone, Marseille, France Department of Psychiatry, Division of Systems Neuroscience, Columbia University and Research Foundation for Mental Hygiene, New York State Psychiatric Institute, New York, NY 10032, United States Laboratory of 3D Functional Network and Dendritic Imaging, Institute of Experimental Medicine, Hungarian Academy of Sciences, Budapest, H-1083, Hungary Aix Marseille Univ, INSERM, INS, Inst Neurosci Syst, Marseille, France Neuroengineering Research Group, Interdisciplinary Excellence Center, Department of Medical Microbiology and Immunobiology, University of Szeged, Szeged, 6720, Hungary Two-Photon Measurement Technology Research Group, Pázmány Péter Catholic University, Budapest, H-1083, Hungary Electrical Engineering Division, Department of Engineering, University of Cambridge, Cambridge, CB3 0FA, United Kingdom Export Date: 17 April 2020 Correspondence Address: Williamson, A.; Aix- Marseille Université, Inserm U1106 INS, Institut de Neurosciences des SystèmesFrance; email: adam.williamson@univ-amu.fr Chemicals/CAS: calcium, 7440-70-2, 14092-94-5; fampridine, 1003-40-3, 504-24-5 Tradenames: Clevios, Heraeus, Germany Manufacturers: Femtonics; Heraeus, Germany; Scientifica Funding details: Université Pierre et Marie Curie, UPMC, 625372 Funding details: European Research Council, ERC Funding details: Horizon 2020 Framework Programme, H2020, 716867 Funding details: Fondation pour la Recherche Médicale, FRM, DBS20131128446, KFI-2016-0177, GINOP-2016-00979, NVKP-2016-0043, ERC682426 Funding text 1: This work was supported by the European Research Council (ERC) under the European Union’s Horizon 2020 Research and Innovation Programme (Grant 716867). A.K. was supported by EC Marie Curie Intra-European Fellowship (ImagINE, Grant 625372). M.J.D. was supported by the Fondation pour la Recherche Médicale Grant DBS20131128446. B.R. and K.G. were supported by ERC682426, KFI-2016-0177, GINOP-2016-00979, and NVKP-2016-0043. *M.J.D. and A.K. contributed equally to this work. Department of Bioelectronics, Ecole Nationale Supérieure des Mines, Centre of Microelectronics in Provence, Gardanne, 13541, France Aix Marseille Univ, CNRS, INT, Inst Neurosci Timone, Marseille, France Department of Psychiatry, Division of Systems Neuroscience, Columbia University and Research Foundation for Mental Hygiene, New York State Psychiatric Institute, New York, NY 10032, United States Laboratory of 3D Functional Network and Dendritic Imaging, Institute of Experimental Medicine, Hungarian Academy of Sciences, Budapest, H-1083, Hungary Aix Marseille Univ, INSERM, INS, Inst Neurosci Syst, Marseille, France Neuroengineering Research Group, Interdisciplinary Excellence Center, Department of Medical Microbiology and Immunobiology, University of Szeged, Szeged, 6720, Hungary Two-Photon Measurement Technology Research Group, Pázmány Péter Catholic University, Budapest, H-1083, Hungary Electrical Engineering Division, Department of Engineering, University of Cambridge, Cambridge, CB3 0FA, United Kingdom Export Date: 22 April 2020 Correspondence Address: Williamson, A.; Aix- Marseille Université, Inserm U1106 INS, Institut de Neurosciences des SystèmesFrance; email: adam.williamson@univ-amu.fr Chemicals/CAS: calcium, 7440-70-2, 14092-94-5; fampridine, 1003-40-3, 504-24-5 Tradenames: Clevios, Heraeus, Germany Manufacturers: Femtonics; Heraeus, Germany; Scientifica Funding details: Université Pierre et Marie Curie, UPMC, 625372 Funding details: European Research Council, ERC Funding details: Horizon 2020 Framework Programme, H2020, 716867 Funding details: Fondation pour la Recherche Médicale, FRM, DBS20131128446, KFI-2016-0177, GINOP-2016-00979, NVKP-2016-0043, ERC682426 Funding text 1: This work was supported by the European Research Council (ERC) under the European Union’s Horizon 2020 Research and Innovation Programme (Grant 716867). A.K. was supported by EC Marie Curie Intra-European Fellowship (ImagINE, Grant 625372). M.J.D. was supported by the Fondation pour la Recherche Médicale Grant DBS20131128446. B.R. and K.G. were supported by ERC682426, KFI-2016-0177, GINOP-2016-00979, and NVKP-2016-0043. *M.J.D. and A.K. contributed equally to this work. Department of Bioelectronics, Ecole Nationale Supérieure des Mines, Centre of Microelectronics in Provence, Gardanne, 13541, France Aix Marseille Univ, CNRS, INT, Inst Neurosci Timone, Marseille, France Department of Psychiatry, Division of Systems Neuroscience, Columbia University and Research Foundation for Mental Hygiene, New York State Psychiatric Institute, New York, NY 10032, United States Laboratory of 3D Functional Network and Dendritic Imaging, Institute of Experimental Medicine, Hungarian Academy of Sciences, Budapest, H-1083, Hungary Aix Marseille Univ, INSERM, INS, Inst Neurosci Syst, Marseille, France Neuroengineering Research Group, Interdisciplinary Excellence Center, Department of Medical Microbiology and Immunobiology, University of Szeged, Szeged, 6720, Hungary Two-Photon Measurement Technology Research Group, Pázmány Péter Catholic University, Budapest, H-1083, Hungary Electrical Engineering Division, Department of Engineering, University of Cambridge, Cambridge, CB3 0FA, United Kingdom Cited By :1 Export Date: 17 March 2021 Correspondence Address: Williamson, A.; Aix- Marseille Université, France; email: adam.williamson@univ-amu.fr Chemicals/CAS: calcium, 7440-70-2, 14092-94-5; fampridine, 1003-40-3, 504-24-5 Tradenames: Clevios, Heraeus, Germany Manufacturers: Femtonics; Heraeus, Germany; Scientifica Funding details: Horizon 2020 Framework Programme, H2020, 682426, 716867 Funding details: Seventh Framework Programme, FP7, 625372 Funding details: European Research Council, ERC Funding details: Fondation pour la Recherche Médicale, FRM, DBS20131128446, ERC682426, GINOP-2016-00979, KFI-2016-0177, NVKP-2016-0043 Funding details: Université Pierre et Marie Curie, UPMC Funding text 1: This work was supported by the European Research Council (ERC) under the European Union’s Horizon 2020 Research and Innovation Programme (Grant 716867). A.K. was supported by EC Marie Curie Intra-European Fellowship (ImagINE, Grant 625372). M.J.D. was supported by the Fondation pour la Recherche Médicale Grant DBS20131128446. B.R. and K.G. were supported by ERC682426, KFI-2016-0177, GINOP-2016-00979, and NVKP-2016-0043. *M.J.D. and A.K. contributed equally to this work. Department of Bioelectronics, Ecole Nationale Supérieure des Mines, Centre of Microelectronics in Provence, Gardanne, 13541, France Aix Marseille Univ, CNRS, INT, Inst Neurosci Timone, Marseille, France Department of Psychiatry, Division of Systems Neuroscience, Columbia University and Research Foundation for Mental Hygiene, New York State Psychiatric Institute, New York, NY 10032, United States Laboratory of 3D Functional Network and Dendritic Imaging, Institute of Experimental Medicine, Hungarian Academy of Sciences, Budapest, H-1083, Hungary Aix Marseille Univ, INSERM, INS, Inst Neurosci Syst, Marseille, France Neuroengineering Research Group, Interdisciplinary Excellence Center, Department of Medical Microbiology and Immunobiology, University of Szeged, Szeged, 6720, Hungary Two-Photon Measurement Technology Research Group, Pázmány Péter Catholic University, Budapest, H-1083, Hungary Electrical Engineering Division, Department of Engineering, University of Cambridge, Cambridge, CB3 0FA, United Kingdom Cited By :1 Export Date: 20 April 2021 Correspondence Address: Williamson, A.; Aix- Marseille Université, France; email: adam.williamson@univ-amu.fr Chemicals/CAS: calcium, 7440-70-2, 14092-94-5; fampridine, 1003-40-3, 504-24-5 Tradenames: Clevios, Heraeus, Germany Manufacturers: Femtonics; Heraeus, Germany; Scientifica Funding details: Horizon 2020 Framework Programme, H2020, 682426, 716867 Funding details: Seventh Framework Programme, FP7, 625372 Funding details: European Research Council, ERC Funding details: Fondation pour la Recherche Médicale, FRM, DBS20131128446, ERC682426, GINOP-2016-00979, KFI-2016-0177, NVKP-2016-0043 Funding text 1: This work was supported by the European Research Council (ERC) under the European Union’s Horizon 2020 Research and Innovation Programme (Grant 716867). A.K. was supported by EC Marie Curie Intra-European Fellowship (ImagINE, Grant 625372). M.J.D. was supported by the Fondation pour la Recherche Médicale Grant DBS20131128446. B.R. and K.G. were supported by ERC682426, KFI-2016-0177, GINOP-2016-00979, and NVKP-2016-0043. *M.J.D. and A.K. contributed equally to this work. AB - Transparent and flexible materials are attractive for a wide range of emerging bioelectronic applications. These include neural interfacing devices for both recording and stimulation, where low electrochemical electrode impedance is valuable. Here the conducting polymer poly(3,4-ethylenedioxythiophene): poly(styrenesulfonate) (PEDOT:PSS) is utilized to fabricate electrodes that are small enough to allow unencumbered optical access for imaging a large cell population with two-photon (2P) microscopy, yet provide low impedance for simultaneous high quality recordings of neural activity in vivo. To demonstrate this, pathophysiological activity was induced in the mouse cortex using 4-aminopyridine (4AP) and the resulting electrical activity was detected with the PEDOT:PSS-based probe while imaging calcium activity directly below the probe area. The induced calcium activity of the neuronal network as measured by the fluorescence change in the cells correlated well with the electrophysiological recordings from the cortical grid of PEDOT:PSS microelectrodes. Our approach provides a valuable vehicle for complementing classical high temporal resolution electrophysiological analysis with optical imaging. LA - English DB - MTMT ER - TY - JOUR AU - Szalay, Gergely AU - Sulcz-Judák, Linda AU - Katona, Gergely AU - Ócsai, Katalin AU - Juhász, Gábor AU - Veress, Máté AU - Szadai, Zoltán AU - Fehér, András AU - Tompa, Tamás AU - Chiovini, Balázs AU - Maák, Pál AU - Rózsa J., Balázs TI - Fast 3D Imaging of Spine, Dendritic, and Neuronal Assemblies in Behaving Animals. JF - NEURON J2 - NEURON VL - 92 PY - 2016 IS - 4 SP - 723 EP - 738 PG - 16 SN - 0896-6273 DO - 10.1016/j.neuron.2016.10.002 UR - https://m2.mtmt.hu/api/publication/3144889 ID - 3144889 AB - Understanding neural computation requires methods such as 3D acousto-optical (AO) scanning that can simultaneously read out neural activity on both the somatic and dendritic scales. AO point scanning can increase measurement speed and signal- to-noise ratio (SNR) by several orders of magnitude, but high optical resolution requires long point-to-point switching time, which limits imaging capability. Here we present a novel technology, 3D DRIFT AO scanning, which can extend each scanning point to small 3D lines, surfaces, or volume elements for flexible and fast imaging of complex structures simultaneously in multiple locations. Our method was demonstrated by fast 3D recording of over 150 dendritic spines with 3D lines, over 100 somata with squares and cubes, or multiple spiny dendritic segments with surface and volume elements, including in behaving animals. Finally, a 4-fold improvement in total excitation efficiency resulted in about 500 x 500 x 650 mum scanning volume with genetically encoded calcium indicators (GECIs). LA - English DB - MTMT ER -