@article{MTMT:30382220, title = {Multimodal Characterization of Neural Networks using Highly Transparent Electrode Arrays}, url = {https://m2.mtmt.hu/api/publication/30382220}, author = {Donahue, Mary J. and Kaszás, Attila and Turi, Gergely F. and Rózsa J., Balázs and Slézia, Andrea and Vanzetta, Ivo and Katona, Gergely and Bernard, Christophe and Malliaras, George G. and Williamson, Adam (John)}, doi = {10.1523/ENEURO.0187-18.2018}, journal-iso = {ENEURO}, journal = {ENEURO}, volume = {5}, unique-id = {30382220}, abstract = {Transparent and flexible materials are attractive for a wide range of emerging bioelectronic applications. These include neural interfacing devices for both recording and stimulation, where low electrochemical electrode impedance is valuable. Here the conducting polymer poly(3,4-ethylenedioxythiophene): poly(styrenesulfonate) (PEDOT:PSS) is utilized to fabricate electrodes that are small enough to allow unencumbered optical access for imaging a large cell population with two-photon (2P) microscopy, yet provide low impedance for simultaneous high quality recordings of neural activity in vivo. To demonstrate this, pathophysiological activity was induced in the mouse cortex using 4-aminopyridine (4AP) and the resulting electrical activity was detected with the PEDOT:PSS-based probe while imaging calcium activity directly below the probe area. The induced calcium activity of the neuronal network as measured by the fluorescence change in the cells correlated well with the electrophysiological recordings from the cortical grid of PEDOT:PSS microelectrodes. Our approach provides a valuable vehicle for complementing classical high temporal resolution electrophysiological analysis with optical imaging.}, year = {2018}, eissn = {2373-2822}, orcid-numbers = {Rózsa J., Balázs/0000-0003-1427-7003; Slézia, Andrea/0000-0002-4528-3169; Katona, Gergely/0000-0002-4173-0355} } @article{MTMT:3144889, title = {Fast 3D Imaging of Spine, Dendritic, and Neuronal Assemblies in Behaving Animals.}, url = {https://m2.mtmt.hu/api/publication/3144889}, author = {Szalay, Gergely and Sulcz-Judák, Linda and Katona, Gergely and Ócsai, Katalin and Juhász, Gábor and Veress, Máté and Szadai, Zoltán and Fehér, András and Tompa, Tamás and Chiovini, Balázs and Maák, Pál and Rózsa J., Balázs}, doi = {10.1016/j.neuron.2016.10.002}, journal-iso = {NEURON}, journal = {NEURON}, volume = {92}, unique-id = {3144889}, issn = {0896-6273}, abstract = {Understanding neural computation requires methods such as 3D acousto-optical (AO) scanning that can simultaneously read out neural activity on both the somatic and dendritic scales. AO point scanning can increase measurement speed and signal- to-noise ratio (SNR) by several orders of magnitude, but high optical resolution requires long point-to-point switching time, which limits imaging capability. Here we present a novel technology, 3D DRIFT AO scanning, which can extend each scanning point to small 3D lines, surfaces, or volume elements for flexible and fast imaging of complex structures simultaneously in multiple locations. Our method was demonstrated by fast 3D recording of over 150 dendritic spines with 3D lines, over 100 somata with squares and cubes, or multiple spiny dendritic segments with surface and volume elements, including in behaving animals. Finally, a 4-fold improvement in total excitation efficiency resulted in about 500 x 500 x 650 mum scanning volume with genetically encoded calcium indicators (GECIs).}, year = {2016}, eissn = {1097-4199}, pages = {723-738}, orcid-numbers = {Katona, Gergely/0000-0002-4173-0355; Rózsa J., Balázs/0000-0003-1427-7003} }