TY  - JOUR
AU  - Cossarizza, A.
AU  - Chang, H.-D.
AU  - Radbruch, A.
AU  - Acs, A.
AU  - Adam, D.
AU  - Adam-Klages, S.
AU  - Agace, W.W.
AU  - Aghaeepour, N.
AU  - Akdis, M.
AU  - Allez, M.
AU  - Almeida, L.N.
AU  - Alvisi, G.
AU  - Anderson, G.
AU  - Andrä, I.
AU  - Annunziato, F.
AU  - Anselmo, A.
AU  - Bacher, P.
AU  - Baldari, C.T.
AU  - Bari, S.
AU  - Barnaba, V.
AU  - Barros-Martins, J.
AU  - Battistini, L.
AU  - Bauer, W.
AU  - Baumgart, S.
AU  - Baumgarth, N.
AU  - Baumjohann, D.
AU  - Baying, B.
AU  - Bebawy, M.
AU  - Becher, B.
AU  - Beisker, W.
AU  - Benes, V.
AU  - Beyaert, R.
AU  - Blanco, A.
AU  - Boardman, D.A.
AU  - Bogdan, C.
AU  - Borger, J.G.
AU  - Borsellino, G.
AU  - Boulais, P.E.
AU  - Bradford, J.A.
AU  - Brenner, D.
AU  - Brinkman, R.R.
AU  - Brooks, A.E.S.
AU  - Busch, D.H.
AU  - Büscher, M.
AU  - Bushnell, T.P.
AU  - Calzetti, F.
AU  - Cameron, G.
AU  - Cammarata, I.
AU  - Cao, X.
AU  - Cardell, S.L.
AU  - Casola, S.
AU  - Cassatella, M.A.
AU  - Cavani, A.
AU  - Celada, A.
AU  - Chatenoud, L.
AU  - Chattopadhyay, P.K.
AU  - Chow, S.
AU  - Christakou, E.
AU  - Čičin-Šain, L.
AU  - Clerici, M.
AU  - Colombo, F.S.
AU  - Cook, L.
AU  - Cooke, A.
AU  - Cooper, A.M.
AU  - Corbett, A.J.
AU  - Cosma, A.
AU  - Cosmi, L.
AU  - Coulie, P.G.
AU  - Cumano, A.
AU  - Cvetkovic, L.
AU  - Dang, V.D.
AU  - Dang-Heine, C.
AU  - Davey, M.S.
AU  - Davies, D.
AU  - De, Biasi S.
AU  - Del, Zotto G.
AU  - Dela, Cruz G.V.
AU  - Delacher, M.
AU  - Della, Bella S.
AU  - Dellabona, P.
AU  - Deniz, G.
AU  - Dessing, M.
AU  - Di, Santo J.P.
AU  - Diefenbach, A.
AU  - Dieli, F.
AU  - Dolf, A.
AU  - Dörner, T.
AU  - Dress, R.J.
AU  - Dudziak, D.
AU  - Dustin, M.
AU  - Dutertre, C.-A.
AU  - Ebner, F.
AU  - Eckle, S.B.G.
AU  - Edinger, M.
AU  - Eede, P.
AU  - Ehrhardt, G.R.A.
AU  - Eich, M.
AU  - Engel, P.
AU  - Engelhardt, B.
AU  - Erdei, Anna
AU  - Esser, C.
AU  - Everts, B.
AU  - Evrard, M.
AU  - Falk, C.S.
AU  - Fehniger, T.A.
AU  - Felipo-Benavent, M.
AU  - Ferry, H.
AU  - Feuerer, M.
AU  - Filby, A.
AU  - Filkor, K.
AU  - Fillatreau, S.
AU  - Follo, M.
AU  - Förster, I.
AU  - Foster, J.
AU  - Foulds, G.A.
AU  - Frehse, B.
AU  - Frenette, P.S.
AU  - Frischbutter, S.
AU  - Fritzsche, W.
AU  - Galbraith, D.W.
AU  - Gangaev, A.
AU  - Garbi, N.
AU  - Gaudilliere, B.
AU  - Gazzinelli, R.T.
AU  - Geginat, J.
AU  - Gerner, W.
AU  - Gherardin, N.A.
AU  - Ghoreschi, K.
AU  - Gibellini, L.
AU  - Ginhoux, F.
AU  - Goda, K.
AU  - Godfrey, D.I.
AU  - Goettlinger, C.
AU  - González-Navajas, J.M.
AU  - Goodyear, C.S.
AU  - Gori, A.
AU  - Grogan, J.L.
AU  - Grummitt, D.
AU  - Grützkau, A.
AU  - Haftmann, C.
AU  - Hahn, J.
AU  - Hammad, H.
AU  - Hämmerling, G.
AU  - Hansmann, L.
AU  - Hansson, G.
AU  - Harpur, C.M.
AU  - Hartmann, S.
AU  - Hauser, A.
AU  - Hauser, A.E.
AU  - Haviland, D.L.
AU  - Hedley, D.
AU  - Hernández, D.C.
AU  - Herrera, G.
AU  - Herrmann, M.
AU  - Hess, C.
AU  - Höfer, T.
AU  - Hoffmann, P.
AU  - Hogquist, K.
AU  - Holland, T.
AU  - Höllt, T.
AU  - Holmdahl, R.
AU  - Hombrink, P.
AU  - Houston, J.P.
AU  - Hoyer, B.F.
AU  - Huang, B.
AU  - Huang, F.-P.
AU  - Huber, J.E.
AU  - Huehn, J.
AU  - Hundemer, M.
AU  - Hunter, C.A.
AU  - Hwang, W.Y.K.
AU  - Iannone, A.
AU  - Ingelfinger, F.
AU  - Ivison, S.M.
AU  - Jäck, H.-M.
AU  - Jani, P.K.
AU  - Jávega, B.
AU  - Jonjic, S.
AU  - Kaiser, T.
AU  - Kalina, T.
AU  - Kamradt, T.
AU  - Kaufmann, S.H.E.
AU  - Keller, B.
AU  - Ketelaars, S.L.C.
AU  - Khalilnezhad, A.
AU  - Khan, S.
AU  - Kisielow, J.
AU  - Klenerman, P.
AU  - Knopf, J.
AU  - Koay, H.-F.
AU  - Kobow, K.
AU  - Kolls, J.K.
AU  - Kong, W.T.
AU  - Kopf, M.
AU  - Korn, T.
AU  - Kriegsmann, K.
AU  - Kristyanto, H.
AU  - Kroneis, T.
AU  - Krueger, A.
AU  - Kühne, J.
AU  - Kukat, C.
AU  - Kunkel, D.
AU  - Kunze-Schumacher, H.
AU  - Kurosaki, T.
AU  - Kurts, C.
AU  - Kvistborg, P.
AU  - Kwok, I.
AU  - Landry, J.
AU  - Lantz, O.
AU  - Lanuti, P.
AU  - LaRosa, F.
AU  - Lehuen, A.
AU  - LeibundGut-Landmann, S.
AU  - Leipold, M.D.
AU  - Leung, L.Y.T.
AU  - Levings, M.K.
AU  - Lino, A.C.
AU  - Liotta, F.
AU  - Litwin, V.
AU  - Liu, Y.
AU  - Ljunggren, H.-G.
AU  - Lohoff, M.
AU  - Lombardi, G.
AU  - Lopez, L.
AU  - López-Botet, M.
AU  - Lovett-Racke, A.E.
AU  - Lubberts, E.
AU  - Luche, H.
AU  - Ludewig, B.
AU  - Lugli, E.
AU  - Lunemann, S.
AU  - Maecker, H.T.
AU  - Maggi, L.
AU  - Maguire, O.
AU  - Mair, F.
AU  - Mair, K.H.
AU  - Mantovani, A.
AU  - Manz, R.A.
AU  - Marshall, A.J.
AU  - Martínez-Romero, A.
AU  - Martrus, G.
AU  - Marventano, I.
AU  - Maslinski, W.
AU  - Matarese, G.
AU  - Mattioli, A.V.
AU  - Maueröder, C.
AU  - Mazzoni, A.
AU  - McCluskey, J.
AU  - McGrath, M.
AU  - McGuire, H.M.
AU  - McInnes, I.B.
AU  - Mei, H.E.
AU  - Melchers, F.
AU  - Melzer, S.
AU  - Mielenz, D.
AU  - Miller, S.D.
AU  - Mills, K.H.G.
AU  - Minderman, H.
AU  - Mjösberg, J.
AU  - Moore, J.
AU  - Moran, B.
AU  - Moretta, L.
AU  - Mosmann, T.R.
AU  - Müller, S.
AU  - Multhoff, G.
AU  - Muñoz, L.E.
AU  - Münz, C.
AU  - Nakayama, T.
AU  - Nasi, M.
AU  - Neumann, K.
AU  - Ng, L.G.
AU  - Niedobitek, A.
AU  - Nourshargh, S.
AU  - Núñez, G.
AU  - O'Connor, J.-E.
AU  - Ochel, A.
AU  - Oja, A.
AU  - Ordonez, D.
AU  - Orfao, A.
AU  - Orlowski-Oliver, E.
AU  - Ouyang, W.
AU  - Oxenius, A.
AU  - Palankar, R.
AU  - Panse, I.
AU  - Pattanapanyasat, K.
AU  - Paulsen, M.
AU  - Pavlinic, D.
AU  - Penter, L.
AU  - Peterson, P.
AU  - Peth, C.
AU  - Petriz, J.
AU  - Piancone, F.
AU  - Pickl, W.F.
AU  - Piconese, S.
AU  - Pinti, M.
AU  - Pockley, A.G.
AU  - Podolska, M.J.
AU  - Poon, Z.
AU  - Pracht, K.
AU  - Prinz, I.
AU  - Pucillo, C.E.M.
AU  - Quataert, S.A.
AU  - Quatrini, L.
AU  - Quinn, K.M.
AU  - Radbruch, H.
AU  - Radstake, T.R.D.J.
AU  - Rahmig, S.
AU  - Rahn, H.-P.
AU  - Rajwa, B.
AU  - Ravichandran, G.
AU  - Raz, Y.
AU  - Rebhahn, J.A.
AU  - Recktenwald, D.
AU  - Reimer, D.
AU  - Reis, e Sousa C.
AU  - Remmerswaal, E.B.M.
AU  - Richter, L.
AU  - Rico, L.G.
AU  - Riddell, A.
AU  - Rieger, A.M.
AU  - Robinson, J.P.
AU  - Romagnani, C.
AU  - Rubartelli, A.
AU  - Ruland, J.
AU  - Saalmüller, A.
AU  - Saeys, Y.
AU  - Saito, T.
AU  - Sakaguchi, S.
AU  - Sala-de-Oyanguren, F.
AU  - Samstag, Y.
AU  - Sanderson, S.
AU  - Sandrock, I.
AU  - Santoni, A.
AU  - Sanz, R.B.
AU  - Saresella, M.
AU  - Sautes-Fridman, C.
AU  - Sawitzki, B.
AU  - Schadt, L.
AU  - Scheffold, A.
AU  - Scherer, H.U.
AU  - Schiemann, M.
AU  - Schildberg, F.A.
AU  - Schimisky, E.
AU  - Schlitzer, A.
AU  - Schlosser, J.
AU  - Schmid, S.
AU  - Schmitt, S.
AU  - Schober, K.
AU  - Schraivogel, D.
AU  - Schuh, W.
AU  - Schüler, T.
AU  - Schulte, R.
AU  - Schulz, A.R.
AU  - Schulz, S.R.
AU  - Scottá, C.
AU  - Scott-Algara, D.
AU  - Sester, D.P.
AU  - Shankey, T.V.
AU  - Silva-Santos, B.
AU  - Simon, A.K.
AU  - Sitnik, K.M.
AU  - Sozzani, S.
AU  - Speiser, D.E.
AU  - Spidlen, J.
AU  - Stahlberg, A.
AU  - Stall, A.M.
AU  - Stanley, N.
AU  - Stark, R.
AU  - Stehle, C.
AU  - Steinmetz, T.
AU  - Stockinger, H.
AU  - Takahama, Y.
AU  - Takeda, K.
AU  - Tan, L.
AU  - Tárnok, A.
AU  - Tiegs, G.
AU  - Toldi, Gergely
AU  - Tornack, J.
AU  - Traggiai, E.
AU  - Trebak, M.
AU  - Tree, T.I.M.
AU  - Trotter, J.
AU  - Trowsdale, J.
AU  - Tsoumakidou, M.
AU  - Ulrich, H.
AU  - Urbanczyk, S.
AU  - van, de Veen W.
AU  - van, den Broek M.
AU  - van, der Pol E.
AU  - Van, Gassen S.
AU  - Van, Isterdael G.
AU  - van, Lier R.A.W.
AU  - Veldhoen, M.
AU  - Vento-Asturias, S.
AU  - Vieira, P.
AU  - Voehringer, D.
AU  - Volk, H.-D.
AU  - von, Borstel A.
AU  - von, Volkmann K.
AU  - Waisman, A.
AU  - Walker, R.V.
AU  - Wallace, P.K.
AU  - Wang, S.A.
AU  - Wang, X.M.
AU  - Ward, M.D.
AU  - Ward-Hartstonge, K.A.
AU  - Warnatz, K.
AU  - Warnes, G.
AU  - Warth, S.
AU  - Waskow, C.
AU  - Watson, J.V.
AU  - Watzl, C.
AU  - Wegener, L.
AU  - Weisenburger, T.
AU  - Wiedemann, A.
AU  - Wienands, J.
AU  - Wilharm, A.
AU  - Wilkinson, R.J.
AU  - Willimsky, G.
AU  - Wing, J.B.
AU  - Winkelmann, R.
AU  - Winkler, T.H.
AU  - Wirz, O.F.
AU  - Wong, A.
AU  - Wurst, P.
AU  - Yang, J.H.M.
AU  - Yang, J.
AU  - Yazdanbakhsh, M.
AU  - Yu, L.
AU  - Yue, A.
AU  - Zhang, H.
AU  - Zhao, Y.
AU  - Ziegler, S.M.
AU  - Zielinski, C.
AU  - Zimmermann, J.
AU  - Zychlinsky, A.
TI  - Guidelines for the use of flow cytometry and cell sorting in immunological studies (second edition)
JF  - EUROPEAN JOURNAL OF IMMUNOLOGY
J2  - EUR J IMMUNOL
VL  - 49
PY  - 2019
IS  - 10
SP  - 1457
EP  - 1973
PG  - 517
SN  - 0014-2980
DO  - 10.1002/eji.201970107
UR  - https://m2.mtmt.hu/api/publication/30864881
ID  - 30864881
N1  - Department of Medical and Surgical Sciences for Children and Adults, Univ. of Modena and Reggio Emilia School of Medicine, Modena, Italy            
            Deutsches Rheuma-Forschungszentrum (DRFZ), an Institute of the Leibniz Association, Berlin, Germany            
            Department of Biology, Nikolaus-Fiebiger-Center for Molecular Medicine, Friedrich-Alexander-University Erlangen-Nuremberg, Erlangen, Germany            
            Institut für Immunologie, Christian-Albrechts-Universität zu Kiel, Kiel, Germany            
            Mucosal Immunology group, Department of Health Technology, Technical University of Denmark, Kgs. Lyngby, Denmark            
            Immunology Section, Lund University, Lund, Sweden            
            Swiss Institute of Allergy and Asthma Research (SIAF), University of Zurich, Davos, Switzerland            
            Université de Paris, Institut de Recherche Saint-Louis, INSERM U1160, and Gastroenterology Department, Hôpital Saint-Louis – APHP, Paris, France            
            Institute for Systemic Inflammation Research, University of Luebeck, Luebeck, Germany            
            Laboratory of Translational Immunology, Humanitas Clinical and Research Center, Rozzano, Italy            
            University of Birmingham, Birmingham, United Kingdom            
            Institut für Medizinische Mikrobiologie, Immunologie und Hygiene, Technische Universität München, Munich, Germany            
            Institut für Transfusionsmedizin, Universitätsklinik Schleswig-Holstein, Kiel, Germany            
            Institut für Klinische Molekularbiologie, Christian-Albrechts Universität zu Kiel, Germany            
            Department of Life Sciences, University of Siena, Siena, Italy            
            Dipartimento di Medicina Interna e Specialità Mediche, Sapienza Università di Roma, Rome, Italy            
            Center for Life Nano Science@Sapienza, Istituto Italiano di Tecnologia, Rome, Italy            
            Istituto Pasteur - Fondazione Cenci Bolognetti, Rome, Italy            
            Institute of Immunology, Hannover Medical School, Hannover, Germany            
            IRCCS Fondazione Santa Lucia, Rome, Italy            
            Division of Immunology, Allergy and Infectious Diseases, Department of Dermatology, Medical University of Vienna, Vienna, Austria            
            Center for Comparative Medicine & Dept. Pathology, Microbiology & Immunology, University of California, Davis, CA, United States            
            Institute for Immunology, Faculty of Medicine, Biomedical Center, LMU Munich, Planegg-Martinsried, Germany            
            Genomics Core Facility, European Molecular Biology Laboratory (EMBL), Heidelberg, Germany            
            Discipline of Pharmacy, Graduate School of Health, The University of Technology Sydney, Sydney, NSW, Australia            
            Department of Bioengineering, University of California, Los Angeles, CA, United States            
            Flow Cytometry Laboratory, Institute of Molecular Toxicology and Pharmacology, Helmholtz Zentrum München, German Research Center for Environmental Health, München, Germany            
            Department of Medical Biotechnologies and Translational Medicine, University of Milan, Milan, Italy            
            Department of Biomedical Molecular Biology, Center for Inflammation Research, Ghent University - VIB, Ghent, Belgium            
            Flow Cytometry Core Technologies, UCD Conway Institute, University College Dublin, Dublin, Ireland            
            Department of Surgery, The University of British Columbia, Vancouver, Canada            
            BC Children's Hospital Research Institute, Vancouver, Canada            
            Mikrobiologisches Institut - Klinische Mikrobiologie, Immunologie und Hygiene, Universitätsklinikum Erlangen, Erlangen, Germany            
            Friedrich-Alexander-Universität (FAU) Erlangen-Nürnberg and Medical Immunology Campus Erlangen, Erlangen, Germany            
            Department of Immunology and Pathology, Monash University, Melbourne, VIC, Australia            
            Neuroimmunology and Flow Cytometry Units, Fondazione Santa Lucia IRCCS, Rome, Italy            
            Department of Cell Biology, Albert Einstein College of Medicine, Bronx, NY, United States            
            The Ruth L. and David S. Gottesman Institute for Stem Cell and Regenerative Medicine Research, Bronx, NY, United States            
            Thermo Fisher Scientific, Eugene, OR, United States            
            Luxembourg Institute of Health, Department of Infection and Immunity, Experimental and Molecular Immunology, Esch-sur-Alzette, Luxembourg            
            Odense University Hospital, Odense Research Center for Anaphylaxis, University of Southern Denmark, Department of Dermatology and Allergy Center, Odense, Denmark            
            BD Life Sciences, Ashland, OR, United States            
            Department of Medical Genetics, University of British Columbia, Vancouver, BC, Canada            
            University of Auckland, School of Biological Sciences, Maurice Wilkins Center, Auckland, New Zealand            
            German Center for Infection Research (DZIF), Munich, Germany            
            Focus Group ‘‘Clinical Cell Processing and Purification”, Institute for Advanced Study, Technische Universität München, Munich, Germany            
            Biophysics, R&D Engineering, Miltenyi Biotec GmbH, Bergisch Gladbach, Germany            
            Department of Pediatrics and Shared Resource Laboratories, University of Rochester Medical Center, Rochester, NY, United States            
            University of Verona, Department of Medicine, Section of General Pathology, Verona, Italy            
            Department of Microbiology and Immunology, Peter Doherty Institute for Infection and Immunity, University of Melbourne, Parkville, VIC, Australia            
            National Key Laboratory of Medical Immunology, Nankai University, Tianjin, China            
            Department of Microbiology and Immunology, University of Gothenburg, Gothenburg, Sweden            
            The FIRC Institute of Molecular Oncology (FOM), Milan, Italy            
            National Institute for Health, Migration and Poverty (INMP), Rome, Italy            
            Macrophage Biology Group, School of Biology, University of Barcelona, Barcelona, Spain            
            Université Paris Descartes, Institut National de la Santé et de la Recherche Médicale, Paris, France            
            Isaac and Laura Perlmutter Cancer Center, NYU-Langone Medical CenterNY, United States            
            Divsion of Medical Oncology and Hematology, Princess Margaret Hospital, Toronto, ON, Canada            
            Department of Immunobiology, School of Immunology and Microbial Sciences, King's College London, United Kingdom            
            National Institutes of Health Research Biomedical Research Centre at Guy's and St. Thomas' National Health Service, Foundation Trust and King's College London, United Kingdom            
            Department of Vaccinology and Applied Microbiology, Helmholtz Centre for Infection Research, Braunschweig, Germany            
            IRCCS Fondazione Don Carlo Gnocchi, Milan, Italy            
            Department of Physiopathology and Transplants, University of Milan, Milan, Italy            
            Milan Center for Neuroscience, University of Milano-Bicocca, Milan, Italy            
            Flow Cytometry Core, Humanitas Clinical and Research Center, Milan, Italy            
            Department of Medicine, The University of British Columbia, Vancouver, Canada            
            Department of Pathology, University of Cambridge, Cambridge, United Kingdom            
            Department of Respiratory Sciences, University of Leicester, Leicester, United Kingdom            
            National Cytometry Platform, Luxembourg Institute of Health, Department of Infection and Immunity, Esch-sur-Alzette, Luxembourg            
            Department of Experimental and Clinical Medicine, University of Florence, Florence, Italy            
            de Duve Institute, Université catholique de Louvain, Brussels, Belgium            
            Unit Lymphopoiesis, Department of Immunology, Institut Pasteur, Paris, France            
            Division of Molecular Immunology, Nikolaus-Fiebiger-Center, Dept. of Internal Medicine III, University of Erlangen-Nuremberg, Erlangen, Germany            
            Clinical Research Unit, Berlin Institute of Health (BIH), Charite Universitätsmedizin Berlin, Berlin, Germany            
            Infection and Immunity Program and Department of Biochemistry and Molecular Biology, Biomedicine Discovery Institute, Monash University, Clayton, VIC, Australia            
            Department of Surgery, Medicine, Dentistry and Morphological Sciences, Univ. of Modena and Reggio Emilia, Modena, Italy            
            IRCCS Istituto Giannina Gaslini, Genova, Italy            
            Novo Nordisk Foundation Center for Stem Cell Biology – DanStem, University of Copenhagen, Copenhagen, Denmark            
            Regensburg Center for Interventional Immunology (RCI), Regensburg, Germany            
            Division of Immunology, Transplantation and Infectious Diseases, San Raffaele Scientific Institute, Milan, Italy            
            Istanbul University, Aziz Sancar Institute of Experimental Medicine, Department of Immunology, Istanbul, Turkey            
            Sony Europe Ltd, Weybridge, United Kingdom            
            Charité - Universitätsmedizin Berlin, Laboratory of Innate Immunity, Department of Microbiology, Infectious Diseases and Immunology, Berlin, Germany            
            Berlin Institute of Health (BIH), Berlin, Germany            
            University of Palermo, Central Laboratory of Advanced Diagnosis and Biomedical Research, Department of Biomedicine, Neurosciences and Advanced Diagnostics, Palermo, Italy            
            Flow Cytometry Core Facility, Institute of Experimental Immunology, University of Bonn, Bonn, Germany            
            Dept. Medicine/Rheumatology and Clinical Immunology, Charité Universitätsmedizin Berlin, Germany            
            Kennedy Institute of Rheumatology, University of Oxford, Oxford, United Kingdom            
            Program in Emerging Infectious Disease, Duke-NUS Medical School, Singapore            
            Institute of Immunology, Centre for Infection Medicine, Department of Veterinary Medicine, Freie Universität Berlin, Germany            
            Department of Internal Medicine III, University Hospital Regensburg, Germany            
            Charité – Universitätsmedizin Berlin, corporate member of Freie Universität Berlin, Humboldt-Universität zu Berlin, and Berlin Institute of Health, Department of Neuropathology, Germany            
            Department of Immunology, University of Toronto, Toronto, ON, Canada            
            Heidelberg Institute for Stem Cell Technology and Experimental Medicine (HI-STEM gGmbH), Heidelberg, Germany            
            University of Barcelona, Faculty of Medicine and Health Sciences, Department of Biomedical Sciences, Barcelona, Spain            
            Theodor Kocher Institute, University of Bern, Bern, Switzerland            
            Department of Immunology, University L. Eotvos, Budapest, Hungary            
            Leibniz Research Institute for Environmental Medicine, Düsseldorf, Germany            
            Department of Parasitology, Leiden University Medical Center, Leiden, Netherlands            
            Singapore Immunology Network (SIgN), A*STAR (Agency for Science, Technology and Research), Biopolis, Singapore            
            Institute of Transplant Immunology, Hannover Medical School, MHH, Hannover, Germany            
            Division of Oncology, Washington University School of Medicine, St. Louis, MO, United States            
            Laboratory of Cytomics, Joint Research Unit CIPF-UVEG, Principe Felipe Research Center, Valencia, Spain            
            Experimental Medicine Division, Nuffield Department of Medicine, University of Oxford, Oxford, United Kingdom            
            The Flow Cytometry Core Facility, Faculty of Medical Sciences, Newcastle University, Newcastle upon Tyne, United Kingdom            
            MDQuest Ltd., Szeged, Hungary            
            Institut Necker-Enfants Malades, Université Paris Descartes Sorbonne Paris Cité, Faculté de Médecine, AP-HP, Hôpital Necker Enfants Malades, INSERM U1151-CNRS UMR 8253, Paris, France            
            Department of Medicine I, Medical Center - University of Freiburg, Faculty of Medicine, University of Freiburg, Freiburg, Germany            
            Universitaetsklinikum Freiburg, Lighthouse Core Facility, Zentrum für Translationale Zellforschung, Klinik für Innere Medizin I, Freiburg, Germany            
            Immunology and Environment, LIMES Institute, University of Bonn, Bonn, Germany            
            Owl Biomedical Inc., Santa Barbara, United States            
            John van Geest Cancer Research Centre, Nottingham Trent University, Nottingham, United Kingdom            
            Department of Medicine, Albert Einstein College of Medicine, Bronx, NY, United States            
            Charité – Universitätsmedizin Berlin, corporate member of Freie Universität Berlin, Humboldt-Universität zu Berlin, and Berlin Institute of Health, Department of Dermatology, Venereology and Allergology, Germany            
            Nanobiophotonics Department, Leibniz Institute of Photonic Technology (IPHT), Jena, Germany            
            School of Plant Sciences and Bio5 Institute, University of Arizona, Tucson, United States            
            Honorary Dean of Life Sciences, Henan University, Kaifeng, China            
            Division of Molecular Oncology and Immunology, the Netherlands Cancer Institute, Amsterdam, Netherlands            
            Institute of Experimental Immunology, University of Bonn, Germany            
            Stanford Department of Anesthesiology, Perioperative and Pain Medicine, Stanford University School of MedicineCA, United States            
            Fundação Oswaldo Cruz - Minas, Laboratory of Immunopatology, Belo Horizonte, MG, Brazil            
            Department of Mecicine, University of Massachusetts Medical School, Worcester, MA, United States            
            INGM - Fondazione Istituto Nazionale di Genetica Molecolare “Ronmeo ed Enrica Invernizzi”, Milan, Italy            
            Institute of Immunology, Department of Pathobiology, University of Veterinary Medicine Vienna, Austria            
            Christian Doppler Laboratory for Optimized Prediction of Vaccination Success in Pigs, Institute of Immunology, Department of Pathobiology, University of Veterinary Medicine Vienna, Austria            
            Department of Dermatology, Venereology and Allergology, Charité - Universitätsmedizin Berlin, Berlin, Germany            
            Translational Immunology Institute, SingHealth Duke-NUS Academic Medical Centre, Singapore            
            Department of Chemistry, University of Tokyo, Tokyo, Japan            
            Institute of Technological Sciences, Wuhan University, Wuhan, China            
            Institut für Genetik, Universität zu Köln, Köln, Germany            
            Alicante Institute for Health and Biomedical Research (ISABIAL), Alicante, Spain            
            Networked Biomedical Research Center for Hepatic and Digestive Diseases (CIBERehd), Madrid, Spain            
            Fondazione IRCCS Ca’ Granda, Ospedale Maggiore Policlinico, University of Milan, Italy            
            Cancer Immunology Research, Genentech, South San Francisco, CA, United States            
            Institute of Experimental Immunology, University of Zurich, Zurich, Switzerland            
            Friedrich-Alexander-University Erlangen-Nürnberg (FAU), Department of Medicine 3, Rheumatology and Immunology, Universitätsklinikum Erlangen, Erlangen, Germany            
            Department of Internal Medicine and Pediatrics, Faculty of Medicine and Health Sciences, Zwijnaarde, Belgium            
            German Cancer Research Center (DKFZ), Heidelberg, Germany            
            German Cancer Consortium (DKTK), partner site Berlin, Berlin, Germany            
            Department of Hematology, Oncology, and Tumor Immunology, Charité – Universitätsmedizin Berlin, Campus Virchow Klinikum, Berlin, Germany            
            Department of Medicine and Center for Molecular Medicine at Karolinska University Hospital, Solna, Sweden            
            Murdoch Children's Research Institute, Parkville, VIC, Australia            
            Charité – Universitätsmedizin Berlin, corporate member of Freie Universität Berlin, Humboldt-Universität zu Berlin, Germany            
            Department of Rheumatology and Clinical Immunology, Berlin Institute of Health, Berlin, Germany            
            Flow Cytometry, Houston Methodist Hospital Research Institute, Houston, TX, United States            
            Charité - Universitätsmedizin Berlin, Medical Department I, Division of Gastroenterology, Infectiology and Rheumatology, Berlin, Germany            
            Cytometry Service, Incliva Foundation. Clinic Hospital and Faculty of Medicine, University of Valencia, Valencia, Spain            
            Immunobiology Laboratory, Department of Biomedicine, University and University Hospital Basel, Basel, Switzerland            
            Cambridge Institute of Therapeutic Immunology & Infectious Disease, Jeffrey Cheah Biomedical Centre, University of Cambridge, Cambridge, United Kingdom            
            German Cancer Research Center (DKFZ), Division of Theoretical Systems Biology, Heidelberg, Germany            
            Center for Immunology, University of Minnesota, Minneapolis, MN, United States            
            Karolinska Institutet Biomedicum, Solna, Sweden            
            Department of Chemical & Materials Engineering, New Mexico State University, Las Cruces, NM, United States            
            Rheumatologie/Klinische Immunologie, Klinik für Innere Medizin I und Exzellenzzentrum Entzündungsmedizin, Universitätsklinikum Schleswig-Holstein, Kiel, Germany            
            Institute for Advanced Study (IAS), Shenzhen University, Shenzhen, China            
            Department of Immunology & National Key Laboratory of Medical Molecular Biology, Institute of Basic Medical Sciences, Chinese Academy of Medical Sciences (CAMS) & Peking Union Medical College, Beijing, China            
            Experimental Immunology, Helmholtz Centre for Infection Research, Braunschweig, Germany            
            Department of Hematology, Oncology and Rheumatology, University Heidelberg, Heidelberg, Germany            
            Department of Pathobiology, School of Veterinary Medicine, University of Pennsylvania, Philadelphia, PA, United States            
            Department of Diagnostic Medicine, Clinical and Public Health, Univ. of Modena and Reggio Emilia, Modena, Italy            
            Max Planck Institute for Infection Biology, Berlin, Germany            
            Laboratory of Cytomics, Joint Research Unit CIPF-UVEG, Department of Biochemistry and Molecular Biology, University of Valencia, Valencia, Spain            
            Department of Histology and Embryology/Center for Proteomics, Faculty of Medicine, University of Rijeka, Rijeka, Croatia            
            Department of Paediatric Haematology and Oncology, Second Faculty of Medicine, Charles University, Prague, Czech Republic            
            Jena University Hospital, Institute of Immunology, Jena, Germany            
            Department of Rheumatology and Clinical Immunology, Medical Center - University of Freiburg, Faculty of Medicine, University of Freiburg, Freiburg, Germany            
            Center for Chronic Immunodeficiency, Medical Center - University of Freiburg, Faculty of Medicine, University of Freiburg, Freiburg, Germany            
            Department of Microbiology and Immunology, Yong Loo Lin School of Medicine, National University of Singapore, Singapore            
            Institute of Molecular Health Sciences, ETH Zurich, Zürich, Switzerland            
            Department of Neuropathology, Universitätsklinikum Erlangen, Germany            
            John W Deming Endowed Chair in Internal Medicine, Center for Translational Research in Infection and Inflammation Tulane School of Medicine, New Orleans, LA, United States            
            Department of Neurology, Technical University of MunichMunich, Germany            
            Department of Rheumatology, Leiden University Medical Center, Leiden, Netherlands            
            Division of Cell Biology, Histology & Embryology, Gottfried Schatz Research Center, Medical University of Graz, Graz, Austria            
            Institute for Molecular Medicine, Goethe University Frankfurt, Frankfurt am Main, Germany            
            FACS & Imaging Core Facility, Max Planck Institute for Biology of Ageing, Cologne, Germany            
            Flow & Mass Cytometry Core Facility, Charité - Universitätsmedizin Berlin and Berlin Institute of Health, Berlin, Germany            
            BCRT Flow Cytometry Lab, Berlin-Brandenburg Center for Regenerative Therapies, Charité - Universitätsmedizin Berlin, Germany            
            WPI Immunology Frontier Research Center, Osaka UniversityOsaka, Japan            
            School of Biological Sciences, Nanyang Technological University, Singapore, Singapore            
            INSERM U932, PSL University, Institut Curie, Paris, France            
            Department of Medicine and Aging Sciences, Centre on Aging Sciences and Translational Medicine (Ce.S.I.-Me.T.), University “G. d'Annunzio” of Chieti-Pescara, Chieti, Italy            
            Institut Cochin, CNRS8104, INSERM1016, Department of Endocrinology, Metabolism and Diabetes, Université de Paris, Paris, France            
            Section of Immunology, Vetsuisse Faculty, University of ZurichZurich, Switzerland            
            The Human Immune Monitoring Center (HIMC), Institute for Immunity, Transplantation and Infection, Stanford University School of MedicineCA, United States            
            School of Biomedical Engineering, The University of British Columbia, Vancouver, Canada            
            Caprion Biosciences, ImmuneCarta, Montréal, QC, Canada            
            Inst. f. Med. Mikrobiology and Hospital Hygiene, University of Marburg, Germany            
            King's College London, “Peter Gorer” Department of Immunobiology, London, United Kingdom            
            Beckman Coulter, Inc, Miami, FL, United States            
            IMIM(Hospital de Mar Medical Research Institute), University Pompeu Fabra, Barcelona, Spain            
            Department of Microbial Infection and Immunity, Ohio State University, Columbus, OH, United States            
            Department of Rheumatology, Erasmus MC, University Medical Center Rotterdam, Rotterdam, Netherlands            
            Centre d'Immunophénomique - CIPHE (PHENOMIN), Aix Marseille Université (UMS3367), Inserm (US012), CNRS (UMS3367), Marseille, France            
            Institute of Immunobiology, Kantonsspital St.Gallen, St. Gallen, Switzerland            
            Department of Virus Immunology, Heinrich Pette Institute, Leibniz Institute for Experimental Virology, Hamburg, Germany            
            Institute for Immunity, Transplantation, and Infection, Stanford University School of Medicine, Stanford, CA, United States            
            Flow and Image Cytometry Shared Resource, Roswell Park Comprehensive Cancer Center, Buffalo, NY, United States            
            Fred Hutchinson Cancer Research Center, Vaccine and Infectious Disease Division, Seattle, WA, United States            
            Istituto Clinico Humanitas IRCCS and Humanitas University, Pieve Emanuele, Milan, Italy            
            William Harvey Research Institute, Queen Mary University, London, United Kingdom            
            Department of Immunology, Rady Faculty of Health Sciences, University of Manitoba, Winnipeg, MB, Canada            
            Cytomics Technological Service, Principe Felipe Research Center, Valencia, Spain            
            National Institute of Geriatrics, Rheumatology and Rehabilitation, Department of Pathophysiology and ImmunologyWarsaw, Poland            
            Treg Cell Lab, Dipartimento di Medicina Molecolare e Biotecologie Mediche, Università di Napoli Federico II and Istituto per l'Endocrinologia e l'Oncologia Sperimentale, Consiglio Nazionale delle Ricerche (IEOS-CNR), Napoli, Italy            
            Cell Clearance in Health and Disease Lab, VIB Center for Inflammation Research, Ghent, Belgium            
            Department of Biomedical Molecular Biology, Ghent University, Ghent, Belgium            
            Ramaciotti Facility for Human Systems Biology, and Discipline of Pathology, The University of Sydney, Camperdown, Australia            
            Institute of Infection Immunity and Inflammation, College of Medical Veterinary and Life Sciences, University of Glasgow, Glasgow Biomedical Research Centre, Glasgow, United Kingdom            
            Clinical Trial Center Leipzig, University Leipzig, Leipzig, Germany            
            Interdepartmental Immunobiology Center, Dept. of Microbiology-Immunology, Northwestern Univ. Medical School, Chicago, IL, United States            
            Trinity College Dublin, School of Biochemistry and Immunology, Trinity Biomedical Sciences Institute, Dublin, Ireland            
            Center for Infectious Medicine, Department of Medicine Huddinge, ANA Futura, Karolinska Institutet, Stockholm, Sweden            
            Department of Clinical and Experimental Medine, Linköping University, Linköping, Sweden            
            Abramson Cancer Center Flow Cytometry and Cell Sorting Shared Resource, Perelman School of Medicine at the University of Pennsylvania, Philadelphia, PA, United States            
            Department of Immunology, IRCCS Bambino Gesu Children's Hospital, Rome, Italy            
            Centre for Environmental Research - UFZ, Department Environmental Microbiology, Leipzig, Germany            
            Institute for Innovative Radiotherapy (iRT), Experimental Immune Biology, Helmholtz Zentrum München, Neuherberg, Germany            
            Radiation Immuno-Oncology Group, Center for Translational Cancer Research Technische Universität München (TranslaTUM), Klinikum rechts der Isar, Munich, Germany            
            Department of Immunology, Graduate School of Medicine, Chiba University, Chiba city, Chiba, Japan            
            Institute of Experimental Immunology and Hepatology, University Medical Center Hamburg-Eppendorf, Hamburg, Germany            
            Discipline of Dermatology, University of Sydney, Sydney, NSW, Australia            
            State Key Laboratory of Experimental Hematology, Institute of Hematology, Chinese Academy of Medical Sciences & Peking Union Medical College, Tianjin, China            
            Barts and The London School of Medicine and Dentistry, Queen Mary University of London, United Kingdom            
            Department of Pathology and Rogel Cancer Center, the University of Michigan, Ann Arbor, MI, United States            
            Flow Cytometry Core Facility, European Molecular Biology Laboratory (EMBL), Heidelberg, Germany            
            Department of Medicine, Cancer Research Centre (IBMCC-CSIC/USAL), Cytometry Service, University of Salamanca, CIBERONC and Institute for Biomedical Research of Salamanca (IBSAL), Salamanca, Spain            
            Burnet Institute, AMREP Flow Cytometry Core Facility, Melbourne, VIC, Australia            
            Inflammation and Oncology, Research, Amgen Inc, South San Francisco, United States            
            ETH Zurich, Institute of Microbiology, Zurich, Switzerland            
            Department of Transfusion Medicine, Institute of Immunology and Transfusion Medicine, University Medicine Greifswald, Greifswald, Germany            
            Center of Excellence for Flow Cytometry, Department of Research and Development, Faculty of Medicine Siriraj Hospital, Mahidol University, Bangkok, Thailand            
            Institute of Biomedicine and Translational Medicine, University of Tartu, Tartu, Estonia            
            Institute of Immunology, Center for Pathophysiology, Infectiology and Immunology, Medical University of Vienna, Vienna, Austria            
            Department of Life Sciences, University of Modena and Reggio Emilia, Modena, Italy            
            Chromocyte Limited, Electric Works, Sheffield, United Kingdom            
            Department for Internal Medicine 3, Institute for Rheumatology and Immunology, AG Munoz, Universitätsklinikum Erlangen, Erlangen, Germany            
            Department of Immunology, University of Udine, Udine, Italy            
            David H. Smith Center for Vaccine Biology and Immunology, University of Rochester Medical Center, Rochester, NY, United States            
            School of Biomedical and Health Sciences, RMIT University, Bundoora, VIC, Australia            
            Department of Biochemistry and Molecular Biology, Monash University, Clayton, VIC, Australia            
            Department of Rheumatology and Clinical Immunology, University Medical Center Utrecht, Utrecht University, Utrecht, Netherlands            
            Regeneration in Hematopoiesis, Leibniz-Institute on Aging, Fritz-Lipmann-Institute (FLI), Jena, Germany            
            Faculty of Biological Sciences, Friedrich Schiller University Jena, Jena, Germany            
            Preparative Flow Cytometry, Max-Delbrück-Centrum für Molekulare Medizin, Berlin, Germany            
            Bindley Biosciences Center, Purdue University, West Lafayette, IN, United States            
            Department of Internal Medicine, Groene Hart Hospital, Gouda, Netherlands            
            Desatoya LLC, Reno, NV, United States            
            Immunobiology Laboratory, The Francis Crick Institute, London, United Kingdom            
            Department of Experimental Immunology, Amsterdam Infection and Immunity Institute, Amsterdam UMC, University of Amsterdam, Amsterdam, Netherlands            
            Renal Transplant Unit, Division of Internal Medicine, Academic Medical Centre, Amsterdam UMC, University of Amsterdam, Amsterdam, Netherlands            
            Core Facility Flow Cytometry, Biomedical Center, Ludwig-Maximilians-University Munich, Germany            
            Functional Cytomics Group, Josep Carreras Leukaemia Research Institute, Campus ICO-Germans Trias i Pujol, Universitat Autònoma de Barcelona, UAB, Badalona, Spain            
            Flow Cytometry Scientific Technology Platform, The Francis Crick Institute, London, United Kingdom            
            Department of Medical Microbiology and Immunology, University of AlbertaAB, Canada            
            Purdue University Cytometry Laboratories, Purdue University, West Lafayette, IN, United States            
            Cell Biology Unit, IRCCS Ospedale Policlinico San Martino, Genova, Italy            
            Institut für Klinische Chemie und Pathobiochemie, Fakultät für Medizin, Technische Universität München, München, Germany            
            RIKEN Center for Integrative Medical Sciences, Yokohama, Japan            
            Flow Cytometry Facility, Ludwig Cancer Institute, Faculty of Medicine and Biology, University of Lausanne, Epalinges, Switzerland            
            Heidelberg University, Institute of Immunology, Section of Molecular Immunology, Heidelberg, Germany            
            Translational Immunology Laboratory, NIHR BRC, University of Oxford, Kennedy Institute of Rheumatology, Oxford, United Kingdom            
            Innate Immunty Unit, Department of Immunology, Institut Pasteur, Paris, France            
            Institut Pasteur, Inserm U1223, Paris, France            
            Department of Molecular Medicine, Sapienza University of Rome, IRCCS, Neuromed, Pozzilli, Italy            
            Centre de Recherches des Cordeliers, Equipe Cancer et Immunité anti-tumorale, Paris, France            
            Charité – Universitätsmedizin Berlin, and Berlin Institute of Health, Institute of Medical Immunology, Berlin, Germany            
            Comprehensive Cancer Center Zurich, Switzerland            
            Clinic for Orthopedics and Trauma Surgery, University Hospital Bonn, Bonn, Germany            
            R&D Reagents, Miltenyi Biotec GmbH, Bergisch Gladbach, Germany            
            Quantitative Systems Biology, Life & Medical Sciences Institute, University of Bonn, Bonn, Germany            
            Internal Medicine I, University Hospital Regensburg, Germany            
            Flow Cytometry Core Facility, German Cancer Research Centre (DKFZ), Heidelberg, Germany            
            Genome Biology Unit, European Molecular Biology Laboratory (EMBL), Heidelberg, Germany            
            Institute of Molecular and Clinical Immunology, Otto-von-Guericke University, Magdeburg, Germany            
            University of Cambridge, Cambridge Institute for Medical Research, Cambridge, United Kingdom            
            Institut Pasteur, Cellular Lymphocytes Biology, Immunology Departement, Paris, France            
            TRI Flow Cytometry Suite (TRI.fcs), Translational Research Institute, Wooloongabba, QLD, Australia            
            AsedaSciences, West Lafayette, IN, United States            
            Instituto de Medicina Molecular João Lobo Antunes, Faculdade de Medicina, Universidade de Lisboa, Portugal            
            Dept. Molecular Translational Medicine, University of Brescia, Brescia, Italy            
            Department of Oncology, University of Lausanne and CHUV, Epalinges, Switzerland            
            Lundberg Laboratory for Cancer, Department of Pathology, Sahlgrenska Academy at University of Gothenburg, Gothenburg, Sweden            
            BD Life Sciences, San Diego, CA, United States            
            Department of Hematopoiesis, Sanquin Research and Landsteiner Laboratory, Amsterdam UMC, University of Amsterdam, Amsterdam, Netherlands            
            Institute for Hygiene and Applied Immunology, Center for Pathophysiology, Infectiology and Immunology, Medical University of Vienna, Vienna, Austria            
            National Institutes of Health, Bethesda, MD, United States            
            Departement for Therapy Validation, Fraunhofer Institute for Cell Therapy and Immunology IZI, Leipzig, Germany            
            Institute for Medical Informatics, Statistics and Epidemiology (IMISE), University of Leipzig, Leipzig, Germany            
            BioGenes GmbH, Berlin, Germany            
            Novartis Biologics Center, Mechanistic Immunology Unit, Novartis Institute for Biomedical Research, NIBR, Basel, Switzerland            
            Department of Cellular and Molecular Physiology, Penn State University College of MedicinePA, United States            
            Institute of Bioinnovation, BSRC Alexander Fleming, Vari, Greece            
            Department of Biochemistry, Institute of Chemistry, University of São Paulo, São Paulo, SP, Brazil            
            Christine Kühne Center for Allergy Research and Education (CK-CARE), Davos, Switzerland            
            Vesicle Observation Center, Biomedical Engineering & Physics, Laboratory Experimental Clinical Chemistry, Amsterdam University Medical Centers, Location AMC, Netherlands            
            VIB Flow Core, VIB Center for Inflammation Research, Ghent, Belgium            
            Department of Infection Biology, University Hospital Erlangen, Friedrich-Alexander University Erlangen-Nuremberg (FAU), Erlangen, Germany            
            BIH Center for Regenerative Therapies (BCRT) Charité Universitätsmedizin Berlin and Berlin Institute of Health, Core Unit ImmunoCheck, Germany            
            A·P·E-Applied Physics & Electronics, Berlin, Germany            
            Institute for Molecular Medicine, University Medical Center of the Johannes Gutenberg University of Mainz, Mainz, Germany            
            Flow Cytometry Facility, Babraham Institute, Cambridge, United Kingdom            
            Roswell Park Comprehensive Cancer Center, Elm and Carlton Streets, Buffalo, NY, United States            
            The Scientific Platforms, the Westmead Institute for Medical Research, the Westmead Research Hub, Westmead, NSW, Australia            
            Dept of Hematopathology, The University of Texas MD Anderson Cancer Center, Houston, TX, United States            
            Flow Cytometry Core Facility, Blizard Institute, Queen Mary London University, London, United Kingdom            
            Medinfomatics Ltd., West Drayton, Middlesex, United Kingdom            
            Department for Immunology, Leibniz Research Centre for Working Environment and Human Factors at TU Dortmund (IfADo), Dortmund, Germany            
            Institute for Cellular & Molecular Immunology, University Medical Center Göttingen, Göttingen, Germany            
            Department of Infectious Disease, Imperial College London, United Kingdom            
            Wellcome Centre for Infectious Diseases Research in Africa and Department of Medicine, Institute of Infectious Disease and Molecular Medicine, University of Cape Town, South Africa            
            Tuberculosis Laboratory, The Francis Crick Institute, London, United Kingdom            
            Cooperation Unit for Experimental and Translational Cancer Immunology, Institute of Immunology (Charité - Universitätsmedizin Berlin) and German Cancer Research Center (DKFZ), Berlin, Germany            
            University Bonn, Medical Faculty, Bonn, Germany            
            BD Biosciences, San Jose, CA, United States            
            Department of Rheumatology and Immunology, West China Hospital, Sichuan University, Chengdu, Sichuan, China            
            Maurice Müller Laboratories (Department of Biomedical Research), Universitätsklinik für Viszerale Chirurgie und Medizin Inselspital, University of Bern, Bern, Switzerland            
            Department of Hematology, Singapore General Hospital, Singapore            
            Division of Medical Sciences, National Cancer Centre Singapore, Singapore            
            Cancer & Stem Cell Biology, Duke-NUS Medical School, Singapore            
            Executive Offices, National Cancer Centre Singapore, Singapore            
            Leiden Computational Biology Center,
LA  - English
DB  - MTMT
ER  - 

TY  - JOUR
AU  - Théry, C.
AU  - Witwer, K.W.
AU  - Aikawa, E.
AU  - Alcaraz, M.J.
AU  - Anderson, J.D.
AU  - Andriantsitohaina, R.
AU  - Antoniou, A.
AU  - Arab, T.
AU  - Archer, F.
AU  - Atkin-Smith, G.K.
AU  - Ayre, D.C.
AU  - Bach, J.-M.
AU  - Bachurski, D.
AU  - Baharvand, H.
AU  - Balaj, L.
AU  - Baldacchino, S.
AU  - Bauer, N.N.
AU  - Baxter, A.A.
AU  - Bebawy, M.
AU  - Beckham, C.
AU  - Bedina, Zavec A.
AU  - Benmoussa, A.
AU  - Berardi, A.C.
AU  - Bergese, P.
AU  - Bielska, E.
AU  - Blenkiron, C.
AU  - Bobis-Wozowicz, S.
AU  - Boilard, E.
AU  - Boireau, W.
AU  - Bongiovanni, A.
AU  - Borràs, F.E.
AU  - Bosch, S.
AU  - Boulanger, C.M.
AU  - Breakefield, X.
AU  - Breglio, A.M.
AU  - Brennan, M.Á.
AU  - Brigstock, D.R.
AU  - Brisson, A.
AU  - Broekman, M.L.D.
AU  - Bromberg, J.F.
AU  - Bryl-Górecka, P.
AU  - Buch, S.
AU  - Buck, A.H.
AU  - Burger, D.
AU  - Busatto, S.
AU  - Buschmann, D.
AU  - Bussolati, B.
AU  - Buzás, Edit Irén
AU  - Byrd, J.B.
AU  - Camussi, G.
AU  - Carter, D.R.F.
AU  - Caruso, S.
AU  - Chamley, L.W.
AU  - Chang, Y.-T.
AU  - Chaudhuri, A.D.
AU  - Chen, C.
AU  - Chen, S.
AU  - Cheng, L.
AU  - Chin, A.R.
AU  - Clayton, A.
AU  - Clerici, S.P.
AU  - Cocks, A.
AU  - Cocucci, E.
AU  - Coffey, R.J.
AU  - Cordeiro-da-Silva, A.
AU  - Couch, Y.
AU  - Coumans, F.A.W.
AU  - Coyle, B.
AU  - Crescitelli, R.
AU  - Criado, M.F.
AU  - D’Souza-Schorey, C.
AU  - Das, S.
AU  - de, Candia P.
AU  - De, Santana E.F. Jr.
AU  - De, Wever O.
AU  - del, Portillo H.A.
AU  - Demaret, T.
AU  - Deville, S.
AU  - Devitt, A.
AU  - Dhondt, B.
AU  - Di, Vizio D.
AU  - Dieterich, L.C.
AU  - Dolo, V.
AU  - Dominguez, Rubio A.P.
AU  - Dominici, M.
AU  - Dourado, M.R.
AU  - Driedonks, T.A.P.
AU  - Duarte, F.V.
AU  - Duncan, H.M.
AU  - Eichenberger, R.M.
AU  - Ekström, K.
AU  - EL, Andaloussi S.
AU  - Elie-Caille, C.
AU  - Erdbrügger, U.
AU  - Falcón-Pérez, J.M.
AU  - Fatima, F.
AU  - Fish, J.E.
AU  - Flores-Bellver, M.
AU  - Försönits, András
AU  - Frelet-Barrand, A.
AU  - Fricke, F.
AU  - Fuhrmann, G.
AU  - Gabrielsson, S.
AU  - Gámez-Valero, A.
AU  - Gardiner, C.
AU  - Gärtner, K.
AU  - Gaudin, R.
AU  - Gho, Y.S.
AU  - Giebel, B.
AU  - Gilbert, C.
AU  - Gimona, M.
AU  - Giusti, I.
AU  - Goberdhan, D.C.I.
AU  - Görgens, A.
AU  - Gorski, S.M.
AU  - Greening, D.W.
AU  - Gross, J.C.
AU  - Gualerzi, A.
AU  - Gupta, G.N.
AU  - Gustafson, D.
AU  - Handberg, A.
AU  - Haraszti, R.A.
AU  - Harrison, P.
AU  - Hegyesi, Hargita
AU  - Hendrix, A.
AU  - Hill, A.F.
AU  - Hochberg, F.H.
AU  - Hoffmann, K.F.
AU  - Holder, B.
AU  - Holthofer, H.
AU  - Hosseinkhani, B.
AU  - Hu, G.
AU  - Huang, Y.
AU  - Huber, V.
AU  - Hunt, S.
AU  - Ibrahim, A.G.-E.
AU  - Ikezu, T.
AU  - Inal, J.M.
AU  - Isin, M.
AU  - Ivanova, A.
AU  - Jackson, H.K.
AU  - Jacobsen, S.
AU  - Jay, S.M.
AU  - Jayachandran, M.
AU  - Jenster, G.
AU  - Jiang, L.
AU  - Johnson, S.M.
AU  - Jones, J.C.
AU  - Jong, A.
AU  - Jovanovic-Talisman, T.
AU  - Jung, S.
AU  - Kalluri, R.
AU  - Kano, S.-I.
AU  - Kaur, S.
AU  - Kawamura, Y.
AU  - Keller, E.T.
AU  - Khamari, Delaram
AU  - Khomyakova, E.
AU  - Khvorova, A.
AU  - Kierulf, P.
AU  - Kim, K.P.
AU  - Kislinger, T.
AU  - Klingeborn, M.
AU  - Klinke, D.J. II
AU  - Kornek, M.
AU  - Kosanović, M.M.
AU  - Kovács, Árpád Ferenc
AU  - Krämer-Albers, E.-M.
AU  - Krasemann, S.
AU  - Krause, M.
AU  - Kurochkin, I.V.
AU  - Kusuma, G.D.
AU  - Kuypers, S.
AU  - Laitinen, S.
AU  - Langevin, S.M.
AU  - Languino, L.R.
AU  - Lannigan, J.
AU  - Lässer, C.
AU  - Laurent, L.C.
AU  - Lavieu, G.
AU  - Lázaro-Ibáñez, E.
AU  - Le, Lay S.
AU  - Lee, M.-S.
AU  - Lee, Y.X.F.
AU  - Lemos, D.S.
AU  - Lenassi, M.
AU  - Leszczynska, A.
AU  - Li, I.T.S.
AU  - Liao, K.
AU  - Libregts, S.F.
AU  - Ligeti, Erzsébet
AU  - Lim, R.
AU  - Lim, S.K.
AU  - Linē, A.
AU  - Linnemannstöns, K.
AU  - Llorente, A.
AU  - Lombard, C.A.
AU  - Lorenowicz, M.J.
AU  - Lőrincz, Márton Ákos
AU  - Lötvall, J.
AU  - Lovett, J.
AU  - Lowry, M.C.
AU  - Loyer, X.
AU  - Lu, Q.
AU  - Lukomska, B.
AU  - Lunavat, T.R.
AU  - Maas, S.L.N.
AU  - Malhi, H.
AU  - Marcilla, A.
AU  - Mariani, J.
AU  - Mariscal, J.
AU  - Martens-Uzunova, E.S.
AU  - Martin-Jaular, L.
AU  - Martinez, M.C.
AU  - Martins, V.R.
AU  - Mathieu, M.
AU  - Mathivanan, S.
AU  - Maugeri, M.
AU  - McGinnis, L.K.
AU  - McVey, M.J.
AU  - Meckes, D.G. Jr
AU  - Meehan, K.L.
AU  - Mertens, I.
AU  - Minciacchi, V.R.
AU  - Möller, A.
AU  - Møller, Jørgensen M.
AU  - Morales-Kastresana, A.
AU  - Morhayim, J.
AU  - Mullier, F.
AU  - Muraca, M.
AU  - Musante, L.
AU  - Mussack, V.
AU  - Muth, D.C.
AU  - Myburgh, K.H.
AU  - Najrana, T.
AU  - Nawaz, M.
AU  - Nazarenko, I.
AU  - Nejsum, P.
AU  - Neri, C.
AU  - Neri, T.
AU  - Nieuwland, R.
AU  - Nimrichter, L.
AU  - Nolan, J.P.
AU  - Nolte-’t, Hoen E.N.M.
AU  - Hooten, N.N.
AU  - O’Driscoll, L.
AU  - O’Grady, T.
AU  - O’Loghlen, A.
AU  - Ochiya, T.
AU  - Olivier, M.
AU  - Ortiz, A.
AU  - Ortiz, L.A.
AU  - Osteikoetxea, Xabier
AU  - Ostegaard, O.
AU  - Ostrowski, M.
AU  - Park, J.
AU  - Pegtel, D.M.
AU  - Peinado, H.
AU  - Perut, F.
AU  - Pfaffl, M.W.
AU  - Phinney, D.G.
AU  - Pieters, B.C.H.
AU  - Pink, R.C.
AU  - Pisetsky, D.S.
AU  - Pogge, von Strandmann E.
AU  - Polakovicova, I.
AU  - Poon, I.K.H.
AU  - Powell, B.H.
AU  - Prada, I.
AU  - Pulliam, L.
AU  - Quesenberry, P.
AU  - Radeghieri, A.
AU  - Raffai, R.L.
AU  - Raimondo, S.
AU  - Rak, J.
AU  - Ramirez, M.I.
AU  - Raposo, G.
AU  - Rayyan, M.S.
AU  - Regev-Rudzki, N.
AU  - Ricklefs, F.L.
AU  - Robbins, P.D.
AU  - Roberts, D.D.
AU  - Rodrigues, S.C.
AU  - Rohde, E.
AU  - Rome, S.
AU  - Rouschop, K.M.A.
AU  - Rughetti, A.
AU  - Russell, A.E.
AU  - Saá, P.
AU  - Sahoo, S.
AU  - Salas-Huenuleo, E.
AU  - Sánchez, C.
AU  - Saugstad, J.A.
AU  - Saul, M.J.
AU  - Schiffelers, R.M.
AU  - Schneider, R.
AU  - Schøyen, T.H.
AU  - Scott, A.
AU  - Shahaj, E.
AU  - Sharma, S.
AU  - Shatnyeva, O.
AU  - Shekari, F.
AU  - Shelke, G.V.
AU  - Shetty, A.K.
AU  - Shiba, K.
AU  - Siljander, P.R.-M.
AU  - Silva, A.M.
AU  - Skowronek, A.
AU  - Snyder, O.L. II
AU  - Soares, R.P.
AU  - Sódar, Barbara
AU  - Soekmadji, C.
AU  - Sotillo, J.
AU  - Stahl, P.D.
AU  - Stoorvogel, W.
AU  - Stott, S.L.
AU  - Strasser, E.F.
AU  - Swift, S.
AU  - Tahara, H.
AU  - Tewari, M.
AU  - Timms, K.
AU  - Tiwari, S.
AU  - Tixeira, R.
AU  - Tkach, M.
AU  - Toh, W.S.
AU  - Tomasini, R.
AU  - Torrecilhas, A.C.
AU  - Tosar, J.P.
AU  - Toxavidis, V.
AU  - Urbanelli, L.
AU  - Vader, P.
AU  - van, Balkom B.W.M.
AU  - van, der Grein S.G.
AU  - Van, Deun J.
AU  - van, Herwijnen M.J.C.
AU  - Van, Keuren-Jensen K.
AU  - van, Niel G.
AU  - van, Royen M.E.
AU  - van, Wijnen A.J.
AU  - Vasconcelos, M.H.
AU  - Vechetti, I.J. Jr
AU  - Veit, T.D.
AU  - Vella, L.J.
AU  - Velot, É.
AU  - Verweij, F.J.
AU  - Vestad, B.
AU  - Viñas, J.L.
AU  - Visnovitz, Tamás
AU  - Visnovitzné Dr Vukman, Krisztina
AU  - Wahlgren, J.
AU  - Watson, D.C.
AU  - Wauben, M.H.M.
AU  - Weaver, A.
AU  - Webber, J.P.
AU  - Weber, V.
AU  - Wehman, A.M.
AU  - Weiss, D.J.
AU  - Welsh, J.A.
AU  - Wendt, S.
AU  - Wheelock, A.M.
AU  - Wiener, Zoltán
AU  - Witte, L.
AU  - Wolfram, J.
AU  - Xagorari, A.
AU  - Xander, P.
AU  - Xu, J.
AU  - Yan, X.
AU  - Yáñez-Mó, M.
AU  - Yin, H.
AU  - Yuana, Y.
AU  - Zappulli, V.
AU  - Zarubova, J.
AU  - Žėkas, V.
AU  - Zhang, J.-Y.
AU  - Zhao, Z.
AU  - Zheng, L.
AU  - Zheutlin, A.R.
AU  - Zickler, A.M.
AU  - Zimmermann, P.
AU  - Zivkovic, A.M.
AU  - Zocco, D.
AU  - Zuba-Surma, E.K.
TI  - Minimal information for studies of extracellular vesicles 2018 (MISEV2018): a position statement of the International Society for Extracellular Vesicles and update of the MISEV2014 guidelines
JF  - JOURNAL OF EXTRACELLULAR VESICLES
J2  - J EXTRACELLULAR VESICL
VL  - 7
PY  - 2018
IS  - 1
PG  - 47
SN  - 2001-3078
DO  - 10.1080/20013078.2018.1535750
UR  - https://m2.mtmt.hu/api/publication/30338008
ID  - 30338008
LA  - English
DB  - MTMT
ER  - 

TY  - JOUR
AU  - Biró, Orsolya
AU  - Alasztics, Bálint
AU  - Molvarec, Attila
AU  - Joó, József Gábor
AU  - Nagy, Bálint
AU  - Rigó, János
TI  - Various levels of circulating exosomal total-miRNA and miR-210 hypoxamiR in different forms of pregnancy hypertension
JF  - PREGNANCY HYPERTENSION
J2  - PREGNANCY HYPERTENS
VL  - 10
PY  - 2017
SP  - 207
EP  - 212
PG  - 6
SN  - 2210-7789
DO  - 10.1016/j.preghy.2017.09.002
UR  - https://m2.mtmt.hu/api/publication/3273020
ID  - 3273020
AB  - Introduction: Hypertension is a common complication during pregnancy, affecting 10% of pregnant women worldwide. Several microRNA (miRNA) were shown to be involved in hypertensive disorders of pregnancy. In preeclampsia (PE), placental dysfunction causes the enhanced release of extracellular vesicle-derived miRNAs. The hypoxia-sensitive hsa-mir-210 is the most common PE-associated miRNA, but its exosomal profile has not been investigated. Objectives: Our aims were to measure exosomal total-miRNA concentration and to perform expression analysis of circulating exosomal hsa-miR-210 in women affected by chronic hypertension (CHT) gestational hypertension (GHT) or PE. Materials and methods: We collected plasma samples from women with CHT, GHT, PE (moderate: mPE and severe: sPE) and from normotensive pregnancies. Exosomal miRNAs were extracted and miRNA concentration was measured. RT-PCR was carried out with hsa-miR-210-3p-specific primers and relative expression was calculated using the comparative Ct method. Results: The total-miRNA concentration was different in the disease subgroups, and was significantly higher in mPE and sPE compared to the other groups. We found a significant difference in the relative exosomal hsa-miR-210-3p expression between all hypertensive groups compared to the normotensive samples, but significant upregulation was only observed in case of mPE and sPE patients. Both the level of total-miRNA and hsa-miR-210 expression was higher in case of severe PE. Conclusions: The level of circulating exosomal total-miRNA and hsa-miR-210 was elevated in women with PE, and it was higher in the severe form. We showed that hsa-miR-210 is secreted via exosomes, which may have a role in the pathomechanism of the disease. © 2017 International Society for the Study of Hypertension in Pregnancy.
LA  - English
DB  - MTMT
ER  - 

TY  - JOUR
AU  - Coumans, FAW
AU  - Brisson, AR
AU  - Buzás, Edit Irén
AU  - Dignat-George, F
AU  - Drees, EEE
AU  - El-Andaloussi, S
AU  - Emanueli, C
AU  - Gasecka, A
AU  - Hendrix, A
AU  - Hill, AF
AU  - Lacroix, R
AU  - Lee, Y
AU  - van Leeuwen, TG
AU  - Mackman, N
AU  - Mager, I
AU  - Nolan, JP
AU  - van der Pol, E
AU  - Pegtel, DM
AU  - Sahoo, S
AU  - Siljander, PRM
AU  - Sturk, G
AU  - de Wever, O
AU  - Nieuwland, R
TI  - Methodological Guidelines to Study Extracellular Vesicles
JF  - CIRCULATION RESEARCH
J2  - CIRC RES
VL  - 120
PY  - 2017
IS  - 10
SP  - 1632
EP  - 1648
PG  - 17
SN  - 0009-7330
DO  - 10.1161/CIRCRESAHA.117.309417
UR  - https://m2.mtmt.hu/api/publication/3255872
ID  - 3255872
N1  - Biomedical Engineering and Physics, Academic Medical Center, University of Amsterdam, Amsterdam, Netherlands            
            Vesicle Observation Centre, Academic Medical Center, University of Amsterdam, Amsterdam, Netherlands            
            Laboratory of Experimental Clinical Chemistry, Academic Medical Center, University of Amsterdam, Amsterdam, Netherlands            
            Extracellular Vesicles and Membrane Repair, UMR-5248-CBMN CNRS, University of Bordeaux, Pessac, France            
            Department of Genetics, Cell- and Immunobology, Semmelweis University, Budapest, Hungary            
            VRCM, UMRS-1076, INSERM, Aix-Marseille University, UFR de Pharmacie, Marseille, France            
            Haematology and vascular biology department, CHU La Conception, APHM, Marseille, France            
            Exosomes Research Group, Department of Pathology, VU University Medical Center, De Boelelaan, Amsterdam, 1117, Netherlands            
            Department of Laboratory Medicine, Karolinska Institutet, Stockholm, Sweden            
            Department of Physiology, Anatomy and Genetics, University of Oxford, Oxford, United Kingdom            
            Bristol Heart Institute, University of Bristol, Bristol, United Kingdom            
            National Heart and Lung Institute, Imperial College London, London, United Kingdom            
            St Department and Department of Cardiology, Medical University of Warsaw, Warsaw, Poland            
            Laboratory of Experimental Cancer Research, Department of Radiation Oncology and Experimental Cancer Research, Ghent University, Ghent, Belgium            
            Cancer Research Institute Ghent, Ghent, Belgium            
            Department of Biochemistry and Genetics, La Trobe Institute for Molecular Science, La Trobe University, Bundoora, VIC, Australia            
            Department of Medicine, University of North Carolina at Chapel Hill, Chapel Hill, NC, United States            
            Institute of Technology, University of Tartu, Tartu, Estonia            
            Scintillon Institute, San Diego, CA, United States            
            Cardiovascular Research Center, Icahn School of Medicine at Mount Sinai, New York, NY, United States            
            Division of Biochemistry and Biotechnology, Department of Biosciences, University of Helsinki, Helsinki, Finland            
            Department of Clinical Chemistry, Academic Medical Centre, University of Amsterdam, Meibergdreef 9, Amsterdam, 1105 AZ, Netherlands            
            Cited By :129            
            Export Date: 2 August 2019            
            CODEN: CIRUA            
            Correspondence Address: Nieuwland, R.; Department of Clinical Chemistry, Academic Medical Centre, University of Amsterdam, Meibergdreef 9, Netherlands; email: r.nieuwland@amc.uva.nl
Biomedical Engineering and Physics, Academic Medical Center, University of Amsterdam, Amsterdam, Netherlands            
            Vesicle Observation Centre, Academic Medical Center, University of Amsterdam, Amsterdam, Netherlands            
            Laboratory of Experimental Clinical Chemistry, Academic Medical Center, University of Amsterdam, Amsterdam, Netherlands            
            Extracellular Vesicles and Membrane Repair, UMR-5248-CBMN CNRS, University of Bordeaux, Pessac, France            
            Department of Genetics, Cell- and Immunobology, Semmelweis University, Budapest, Hungary            
            VRCM, UMRS-1076, INSERM, Aix-Marseille University, UFR de Pharmacie, Marseille, France            
            Haematology and vascular biology department, CHU La Conception, APHM, Marseille, France            
            Exosomes Research Group, Department of Pathology, VU University Medical Center, De Boelelaan, Amsterdam, 1117, Netherlands            
            Department of Laboratory Medicine, Karolinska Institutet, Stockholm, Sweden            
            Department of Physiology, Anatomy and Genetics, University of Oxford, Oxford, United Kingdom            
            Bristol Heart Institute, University of Bristol, Bristol, United Kingdom            
            National Heart and Lung Institute, Imperial College London, London, United Kingdom            
            St Department and Department of Cardiology, Medical University of Warsaw, Warsaw, Poland            
            Laboratory of Experimental Cancer Research, Department of Radiation Oncology and Experimental Cancer Research, Ghent University, Ghent, Belgium            
            Cancer Research Institute Ghent, Ghent, Belgium            
            Department of Biochemistry and Genetics, La Trobe Institute for Molecular Science, La Trobe University, Bundoora, VIC, Australia            
            Department of Medicine, University of North Carolina at Chapel Hill, Chapel Hill, NC, United States            
            Institute of Technology, University of Tartu, Tartu, Estonia            
            Scintillon Institute, San Diego, CA, United States            
            Cardiovascular Research Center, Icahn School of Medicine at Mount Sinai, New York, NY, United States            
            Division of Biochemistry and Biotechnology, Department of Biosciences, University of Helsinki, Helsinki, Finland            
            Department of Clinical Chemistry, Academic Medical Centre, University of Amsterdam, Meibergdreef 9, Amsterdam, 1105 AZ, Netherlands            
            Cited By :132            
            Export Date: 5 August 2019            
            CODEN: CIRUA            
            Correspondence Address: Nieuwland, R.; Department of Clinical Chemistry, Academic Medical Centre, University of Amsterdam, Meibergdreef 9, Netherlands; email: r.nieuwland@amc.uva.nl
AB  - Owing to the relationship between extracellular vesicles (EVs) and physiological and pathological conditions, the interest in EVs is exponentially growing. EVs hold high hopes for novel diagnostic and translational discoveries. This review provides an expert-based update of recent advances in the methods to study EVs and summarizes currently accepted considerations and recommendations from sample collection to isolation, detection, and characterization of EVs. Common misconceptions and methodological pitfalls are highlighted. Although EVs are found in all body fluids, in this review, we will focus on EVs from human blood, not only our most complex but also the most interesting body fluid for cardiovascular research.
LA  - English
DB  - MTMT
ER  - 

TY  - JOUR
AU  - van der Pol, E
AU  - Coumans, F
AU  - Varga, Zoltán
AU  - Krumrey, M
AU  - Nieuwland, R
TI  - Innovation in detection of microparticles and exosomes
JF  - JOURNAL OF THROMBOSIS AND HAEMOSTASIS
J2  - J THROMB HAEMOST
VL  - 11
PY  - 2013
SP  - 36
EP  - 45
PG  - 10
SN  - 1538-7933
DO  - 10.1111/jth.12254
UR  - https://m2.mtmt.hu/api/publication/2502232
ID  - 2502232
N1  - Laboratory of Experimental Clinical Chemistry, Amsterdam, Netherlands            
            Biomedical Engineering and Physics, Academic Medical Centre of the University of Amsterdam, Amsterdam, Netherlands            
            Biological Nanochemistry, Institute of Molecular Pharmacology, Research Centre for Natural Sciences of the Hungarian Academy of Sciences, Budapest, Hungary            
            Physikalisch-Technische Bundesanstalt (PTB), Berlin, Germany            
            Cited By :139            
            Export Date: 5 August 2019            
            CODEN: JTHOA            
            Correspondence Address: Nieuwland, R.; Department of Clinical Chemistry, Academic Medical Centre of the University of Amsterdam, Meibergdreef 9, Amsterdam 1105 AZ, Netherlands; email: r.nieuwland@amc.uva.nl
LA  - English
DB  - MTMT
ER  - 

TY  - JOUR
AU  - Witwer, KW
AU  - Buzás, Edit Irén
AU  - Bemis, LT
AU  - Bora, A
AU  - Lasser, C
AU  - Lotvall, J
AU  - Nolte-'t, Hoen EN
AU  - Piper, MG
AU  - Sivaraman, S
AU  - Skog, J
AU  - Thery, C
AU  - Wauben, MH
AU  - Hochberg, F
TI  - Standardization of sample collection, isolation and analysis methods in extracellular vesicle research
JF  - JOURNAL OF EXTRACELLULAR VESICLES
J2  - J EXTRACELLULAR VESICL
VL  - 2
PY  - 2013
PG  - 25
SN  - 2001-3078
DO  - 10.3402/jev.v2i0.20360
UR  - https://m2.mtmt.hu/api/publication/2471567
ID  - 2471567
N1  - Department of Molecular and Comparative Pathobiology, Johns Hopkins University School of Medicine, Baltimore, MD, United States            
            Department of Genetics, Cell- and Immunobiology, Semmelweis University, Budapest, Hungary            
            Department of Biomedical Sciences, University of Minnesota Medical School Duluth, Duluth, MN, United States            
            Department of Pharmacology and Molecular Sciences, Johns Hopkins University School of Medicine, Baltimore, MD, United States            
            Krefting Research Centre, Department of Internal Medicine and Clinical Nutrition, Sahlgrenska Academy, University of Gothenburg, Gothenburg, Sweden            
            Department of Biochemistry and Cell Biology, Faculty of Veterinary Medicine, Utrecht University, Utrecht, Netherlands            
            Department of Internal Medicine, College of Medicine, The Ohio State University, Columbus, OH, United States            
            Massachusetts General Hospital Cancer Center, Boston, MA, United States            
            Exosome Diagnostics, Inc., New York, United States            
            INSERM, Cedex, Paris, France            
            Institut Curie, Paris, France            
            Cited By :807            
            Export Date: 5 August 2019            
            Correspondence Address: Witwer, K.W.733 N. Broadway, Miller Research Building, Baltimore, MD 21205, United States; email: kwitwer1@jhmi.edu
Department of Molecular and Comparative Pathobiology, Johns Hopkins University School of Medicine, Baltimore, MD, United States            
            Department of Genetics, Cell- and Immunobiology, Semmelweis University, Budapest, Hungary            
            Department of Biomedical Sciences, University of Minnesota Medical School Duluth, Duluth, MN, United States            
            Department of Pharmacology and Molecular Sciences, Johns Hopkins University School of Medicine, Baltimore, MD, United States            
            Krefting Research Centre, Department of Internal Medicine and Clinical Nutrition, Sahlgrenska Academy, University of Gothenburg, Gothenburg, Sweden            
            Department of Biochemistry and Cell Biology, Faculty of Veterinary Medicine, Utrecht University, Utrecht, Netherlands            
            Department of Internal Medicine, College of Medicine, The Ohio State University, Columbus, OH, United States            
            Massachusetts General Hospital Cancer Center, Boston, MA, United States            
            Exosome Diagnostics, Inc., New York, United States            
            INSERM, Cedex, Paris, France            
            Institut Curie, Paris, France            
            Cited By :807            
            Export Date: 7 August 2019            
            Correspondence Address: Witwer, K.W.733 N. Broadway, Miller Research Building, Baltimore, MD 21205, United States; email: kwitwer1@jhmi.edu
AB  - The emergence of publications on extracellular RNA (exRNA) and extracellular vesicles (EV) has highlighted the potential of these molecules and vehicles as biomarkers of disease and therapeutic targets. These findings have created a paradigm shift, most prominently in the field of oncology, prompting expanded interest in the field and dedication of funds for EV research. At the same time, understanding of EV subtypes, biogenesis, cargo and mechanisms of shuttling remains incomplete. The techniques that can be harnessed to address the many gaps in our current knowledge were the subject of a special workshop of the International Society for Extracellular Vesicles (ISEV) in New York City in October 2012. As part of the "ISEV Research Seminar: Analysis and Function of RNA in Extracellular Vesicles (evRNA)", 6 round-table discussions were held to provide an evidence-based framework for isolation and analysis of EV, purification and analysis of associated RNA molecules, and molecular engineering of EV for therapeutic intervention. This article arises from the discussion of EV isolation and analysis at that meeting. The conclusions of the round table are supplemented with a review of published materials and our experience. Controversies and outstanding questions are identified that may inform future research and funding priorities. While we emphasize the need for standardization of specimen handling, appropriate normative controls, and isolation and analysis techniques to facilitate comparison of results, we also recognize that continual development and evaluation of techniques will be necessary as new knowledge is amassed. On many points, consensus has not yet been achieved and must be built through the reporting of well-controlled experiments.
LA  - English
DB  - MTMT
ER  - 

TY  - JOUR
AU  - György, Bence
AU  - Szabó, Géza Tamás
AU  - Szente-Pásztói, Mária
AU  - Pál, Zsuzsanna
AU  - Misják, Petra
AU  - Aradi, B
AU  - László, Valéria
AU  - Pállinger, Éva
AU  - Nyitrayné Pap, Erna
AU  - Kittel, Ágnes
AU  - Nagy, György
AU  - Falus, András
AU  - Buzás, Edit Irén
TI  - Membrane vesicles, current state-of-the-art: emerging role of extracellular vesicles
JF  - CELLULAR AND MOLECULAR LIFE SCIENCES
J2  - CELL MOL LIFE SCI
VL  - 68
PY  - 2011
IS  - 16
SP  - 2667
EP  - 2688
PG  - 22
SN  - 1420-682X
DO  - 10.1007/s00018-011-0689-3
UR  - https://m2.mtmt.hu/api/publication/1577931
ID  - 1577931
N1  - B. György and T. G. Szabó contributed equally.
Department of Genetics, Cell- and Immunobiology, Semmelweis University, Budapest, Nagyvárad tér 4, Budapest 1089, Hungary            
Research Group for Inflammation Biology and Immunogenomics, Hungarian Academy of Sciences, Nagyvárad tér 4, Budapest, Hungary            
Institute of Experimental Medicine, Hungarian Academy of Sciences, Szigony utca 43, Budapest, Hungary            
Department of Rheumatology, Semmelweis University, Frankel Leó utca 54, Budapest, Hungary
AB  - Release of membrane vesicles, a process conserved in both prokaryotes and eukaryotes, represents an evolutionary link, and suggests essential functions of a dynamic extracellular vesicular compartment (including exosomes, microparticles or microvesicles and apoptotic bodies). Compelling evidence supports the significance of this compartment in a broad range of physiological and pathological processes. However, classification of membrane vesicles, protocols of their isolation and detection, molecular details of vesicular release, clearance and biological functions are still under intense investigation. Here, we give a comprehensive overview of extracellular vesicles. After discussing the technical pitfalls and potential artifacts of the rapidly emerging field, we compare results from meta-analyses of published proteomic studies on membrane vesicles. We also summarize clinical implications of membrane vesicles. Lessons from this compartment challenge current paradigms concerning the mechanisms of intercellular communication and immune regulation. Furthermore, its clinical implementation may open new perspectives in translational medicine both in diagnostics and therapy.
LA  - English
DB  - MTMT
ER  -