TY - JOUR AU - Kovács, Árpád Ferenc AU - Fekete, Nóra AU - Turiák, Lilla AU - Ács, András AU - Kőhidai, László AU - Buzás, Edit Irén AU - Pállinger, Éva TI - Unravelling the Role of Trophoblastic-Derived Extracellular Vesicles in Regulatory T Cell Differentiation JF - INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES J2 - INT J MOL SCI VL - 20 PY - 2019 IS - 14 PG - 12 SN - 1661-6596 DO - 10.3390/ijms20143457 UR - https://m2.mtmt.hu/api/publication/30807220 ID - 30807220 LA - English DB - MTMT ER - TY - JOUR AU - Kovács, Árpád Ferenc AU - Láng, Orsolya AU - Turiák, Lilla AU - Ács, András AU - Kőhidai, László AU - Fekete, Nóra AU - Alasztics, Bálint AU - Mészáros, Tamás AU - Buzás, Edit Irén AU - Rigó, János AU - Pállinger, Éva TI - The impact of circulating preeclampsia-associated extracellular vesicles on the migratory activity and phenotype of THP-1 monocytic cells JF - SCIENTIFIC REPORTS J2 - SCI REP VL - 8 PY - 2018 IS - 1 PG - 12 SN - 2045-2322 DO - 10.1038/s41598-018-23706-7 UR - https://m2.mtmt.hu/api/publication/3366649 ID - 3366649 N1 - Department of Genetics Cell- and Immunobiology, Semmelweis University, Budapest, Hungary MS Proteomics Research Group, Research Centre for Natural Sciences, Hungarian Academy of Sciences, Budapest, Hungary 1st Department of Obstetrics and Gynaecology, Semmelweis University, Budapest, Hungary Seroscience Ltd, Budapest, Hungary Nanomedicine Research and Education Center, Institute of Pathophysiology, Semmelweis University, Budapest, Hungary MTA-SE Immunoproteogenomics Extracellular Vesicle Research Group, Budapest, Hungary Cited By :22 Export Date: 3 February 2024 Correspondence Address: Kovács, Á.F.; Department of Genetics Cell- and Immunobiology, Hungary; email: kovacs.arpad@med.semmelweis-univ.hu LA - English DB - MTMT ER - TY - JOUR AU - Biró, Orsolya AU - Alasztics, Bálint AU - Molvarec, Attila AU - Joó, József Gábor AU - Nagy, Bálint AU - Rigó, János TI - Various levels of circulating exosomal total-miRNA and miR-210 hypoxamiR in different forms of pregnancy hypertension JF - PREGNANCY HYPERTENSION J2 - PREGNANCY HYPERTENS VL - 10 PY - 2017 SP - 207 EP - 212 PG - 6 SN - 2210-7789 DO - 10.1016/j.preghy.2017.09.002 UR - https://m2.mtmt.hu/api/publication/3273020 ID - 3273020 AB - Introduction: Hypertension is a common complication during pregnancy, affecting 10% of pregnant women worldwide. Several microRNA (miRNA) were shown to be involved in hypertensive disorders of pregnancy. In preeclampsia (PE), placental dysfunction causes the enhanced release of extracellular vesicle-derived miRNAs. The hypoxia-sensitive hsa-mir-210 is the most common PE-associated miRNA, but its exosomal profile has not been investigated. Objectives: Our aims were to measure exosomal total-miRNA concentration and to perform expression analysis of circulating exosomal hsa-miR-210 in women affected by chronic hypertension (CHT) gestational hypertension (GHT) or PE. Materials and methods: We collected plasma samples from women with CHT, GHT, PE (moderate: mPE and severe: sPE) and from normotensive pregnancies. Exosomal miRNAs were extracted and miRNA concentration was measured. RT-PCR was carried out with hsa-miR-210-3p-specific primers and relative expression was calculated using the comparative Ct method. Results: The total-miRNA concentration was different in the disease subgroups, and was significantly higher in mPE and sPE compared to the other groups. We found a significant difference in the relative exosomal hsa-miR-210-3p expression between all hypertensive groups compared to the normotensive samples, but significant upregulation was only observed in case of mPE and sPE patients. Both the level of total-miRNA and hsa-miR-210 expression was higher in case of severe PE. Conclusions: The level of circulating exosomal total-miRNA and hsa-miR-210 was elevated in women with PE, and it was higher in the severe form. We showed that hsa-miR-210 is secreted via exosomes, which may have a role in the pathomechanism of the disease. © 2017 International Society for the Study of Hypertension in Pregnancy. LA - English DB - MTMT ER - TY - JOUR AU - Foster, BP AU - Balassa, Tímea AU - Benen, TD AU - Dominovic, M AU - Elmadjian, GK AU - Florova, V AU - Fransolet, MD AU - Kestlerova, A AU - Kmiecik, G AU - Kostadinova, IA AU - Kyvelidou, C AU - Meggyes, Mátyás AU - Mincheva, MN AU - Moro, L AU - Pastuschek, J AU - Spoldi, V AU - Wandernoth, P AU - Weber, M AU - Toth, B AU - Markert, UR TI - Extracellular vesicles in blood, milk and body fluids of the female and male urogenital tract and with special regard to reproduction JF - CRITICAL REVIEWS IN CLINICAL LABORATORY SCIENCES J2 - CRIT REV CL LAB SCI VL - 53 PY - 2016 IS - 6 SP - 379 EP - 395 PG - 17 SN - 1040-8363 DO - 10.1080/10408363.2016.1190682 UR - https://m2.mtmt.hu/api/publication/3079514 ID - 3079514 AB - Extracellular vesicles (EVs) are released from almost all cells and tissues. They are able to transport substances (e.g. proteins, RNA or DNA) at higher concentrations than in their environment and may adhere in a receptor-controlled manner to specific cells or tissues in order to release their content into the respective target structure. Blood contains high concentrations of EVs mainly derived from platelets, and, at a smaller amount, from erythrocytes. The female and male reproductive tracts produce EVs which may be associated with fertility or infertility and are released into body fluids and mucosas of the urogenital organs. In this review, the currently relevant detection methods are presented and critically compared. During pregnancy, placenta-derived EVs are dynamically detectable in peripheral blood with changing profiles depending upon progress of pregnancy and different pregnancy-associated pathologies, such as preeclampsia. EVs offer novel non-invasive diagnostic tools which may reflect the situation of the placenta and the fetus. EVs in urine have the potential of reflecting urogenital diseases including cancers of the neighbouring organs. Several methods for detection, quantification and phenotyping of EVs have been established, which include electron microscopy, flow cytometry, ELISA-like methods, Western blotting, and analyses based on Brownian motion. This review article summarises the current knowledge about EVs in blood and cord blood, in the different compartments of the male and female reproductive tract, in trophoblast cells from normal and pre-eclamptic pregnancies, in placenta ex vivo perfusate, in the amniotic fluid, and in breast milk, as well as their potential effects on natural killer (NK) cells as possible targets. LA - English DB - MTMT ER -