TY  - JOUR
AU  - Nagy, Bálint
TI  - Cell-free nucleic acids in prenatal diagnosis and pregnancy-associated diseases
JF  - ELECTRONIC JOURNAL OF THE INTERNATIONAL FEDERATION OF CLINICAL CHEMISTRY AND LABORATORY MEDICINE
J2  - E JIFCC
VL  - 30
PY  - 2019
IS  - 2
SP  - 215
EP  - 223
PG  - 9
SN  - 1650-3414
UR  - https://m2.mtmt.hu/api/publication/30758075
ID  - 30758075
N1  - Export Date: 3 August 2019            
            Correspondence Address: Nagy, B.; Department of Human Genetics, Faculty of Medicine, University of Debrecen, Egyetem tér 1, Hungary; email: nagy.balint@med.unideb.hu
AB  - There is a great effort to find out the biological role of cell-free nucleic acids (cfNAs). They are considered very promising targets in the diagnosis of genetic diseases. Non-invasive sampling (liquid biopsy) has recently become a very popular method, and new molecular biological techniques have been developed for these types of samples. Application of next-generation sequencing (NGS) and massively parallel sequencing (MPS) is spreading fast. These are the part of the arsenal of the modern prenatal genetic diagnostic laboratories by now. Cell-free DNA based noninvasive prenatal testing accounts for more than half of the prenatal genetic tests performed, it is gradually replacing the invasive amniocentesis or chorionic villus sample-based diagnostics. Besides that, new non-coding RNAs are taking more attention: microRNAs (miRNAs), long non-coding RNAs (lncRNAs), circular RNAs (circRNAs) are in the focus of the clinical research to detect the most common pregnancy-associated diseases, like preeclampsia, fetal growth restriction, congenital heart diseases and gestational diabetes. The research is at advanced stage on the use of microRNAs, while lncRNAs and circRNAs are still promising targets. In this review, comprehensive information is given about the recent developments on this field.
LA  - English
DB  - MTMT
ER  - 

TY  - JOUR
AU  - Biró, Orsolya
AU  - Alasztics, Bálint
AU  - Molvarec, Attila
AU  - Joó, József Gábor
AU  - Nagy, Bálint
AU  - Rigó, János
TI  - Various levels of circulating exosomal total-miRNA and miR-210 hypoxamiR in different forms of pregnancy hypertension
JF  - PREGNANCY HYPERTENSION
J2  - PREGNANCY HYPERTENS
VL  - 10
PY  - 2017
SP  - 207
EP  - 212
PG  - 6
SN  - 2210-7789
DO  - 10.1016/j.preghy.2017.09.002
UR  - https://m2.mtmt.hu/api/publication/3273020
ID  - 3273020
AB  - Introduction: Hypertension is a common complication during pregnancy, affecting 10% of pregnant women worldwide. Several microRNA (miRNA) were shown to be involved in hypertensive disorders of pregnancy. In preeclampsia (PE), placental dysfunction causes the enhanced release of extracellular vesicle-derived miRNAs. The hypoxia-sensitive hsa-mir-210 is the most common PE-associated miRNA, but its exosomal profile has not been investigated. Objectives: Our aims were to measure exosomal total-miRNA concentration and to perform expression analysis of circulating exosomal hsa-miR-210 in women affected by chronic hypertension (CHT) gestational hypertension (GHT) or PE. Materials and methods: We collected plasma samples from women with CHT, GHT, PE (moderate: mPE and severe: sPE) and from normotensive pregnancies. Exosomal miRNAs were extracted and miRNA concentration was measured. RT-PCR was carried out with hsa-miR-210-3p-specific primers and relative expression was calculated using the comparative Ct method. Results: The total-miRNA concentration was different in the disease subgroups, and was significantly higher in mPE and sPE compared to the other groups. We found a significant difference in the relative exosomal hsa-miR-210-3p expression between all hypertensive groups compared to the normotensive samples, but significant upregulation was only observed in case of mPE and sPE patients. Both the level of total-miRNA and hsa-miR-210 expression was higher in case of severe PE. Conclusions: The level of circulating exosomal total-miRNA and hsa-miR-210 was elevated in women with PE, and it was higher in the severe form. We showed that hsa-miR-210 is secreted via exosomes, which may have a role in the pathomechanism of the disease. © 2017 International Society for the Study of Hypertension in Pregnancy.
LA  - English
DB  - MTMT
ER  -