@article{MTMT:3312483, title = {Comparison of 2D and 3D neural induction methods for the generation of neural progenitor cells from human induced pluripotent stem cells}, url = {https://m2.mtmt.hu/api/publication/3312483}, author = {Chandrasekaran, A and Avci, HX and Ochalek, A and Rosingh, LN and Molnár, Kinga and László, Lajos and Bellák, Tamás and Teglasi, A and Pesti, Krisztina and Mike, Árpád and Phanthong, P and Biró, Orsolya and Hall, V and Kitiyanant, N and Krause, KH and Kobolák, Julianna and Dinnyés, András}, doi = {10.1016/j.scr.2017.10.010}, journal-iso = {STEM CELL RES}, journal = {STEM CELL RESEARCH}, volume = {25}, unique-id = {3312483}, issn = {1873-5061}, abstract = {Neural progenitor cells (NPCs) from human induced pluripotent stem cells (hiPSCs) are frequently induced using 3D culture methodologies however, it is unknown whether spheroid-based (3D) neural induction is actually superior to monolayer (2D) neural induction. Our aim was to compare the efficiency of 2D induction with 3D induction method in their ability to generate NPCs, and subsequently neurons and astrocytes. Neural differentiation was analysed at the protein level qualitatively by immunocytochemistry and quantitatively by flow cytometry for NPC (SOX1, PAX6, NESTIN), neuronal (MAP2, TUBB3), cortical layer (TBR1, CUX1) and glial markers (SOX9, GFAP, AQP4). Electron microscopy demonstrated that both methods resulted in morphologically similar neural rosettes. However, quantification of NPCs derived from 3D neural induction exhibited an increase in the number of PAX6/NESTIN double positive cells and the derived neurons exhibited longer neurites. In contrast, 2D neural induction resulted in more SOX1 positive cells. While 2D monolayer induction resulted in slightly less mature neurons, at an early stage of differentiation, the patch clamp analysis failed to reveal any significant differences between the electrophysiological properties between the two induction methods. In conclusion, 3D neural induction increases the yield of PAX6(+)/NESTIN(+) cells and gives rise to neurons with longer neurites, which might be an advantage for the production of forebrain cortical neurons, highlighting the potential of 3D neural induction, independent of iPSCs' genetic background.}, year = {2017}, eissn = {1876-7753}, pages = {139-151}, orcid-numbers = {Molnár, Kinga/0000-0002-7196-5331; László, Lajos/0000-0002-2114-9109; Mike, Árpád/0000-0002-9095-8161; Biró, Orsolya/0000-0002-4300-3602; Kobolák, Julianna/0000-0002-0986-9517} } @article{MTMT:1789506, title = {Electrophysiological characterization of embryonic hippocampal neurons cultured in a 3D collagen hydrogel}, url = {https://m2.mtmt.hu/api/publication/1789506}, author = {Xu, T and Molnár, Péter and Gregory, C and Das, M and Boland, T and Hickman, JJ}, doi = {10.1016/j.biomaterials.2009.04.047}, journal-iso = {BIOMATERIALS}, journal = {BIOMATERIALS}, volume = {30}, unique-id = {1789506}, issn = {0142-9612}, abstract = {Rat embryonic hippocampal neurons were cultured in (1) 3D collagen hydrogels as 'entrapped' evenly distributed cells, (2) at the interface of two collagen layers (sandwich model), and (3) on the surface of collagen coated coverslips (2D model). In the 'entrapment' model the neuronal processes grew out of the plane of the cell body and extended into the collagen matrix, in contrast to the sandwich model where the cells and their processes rarely left the plane in which they were seeded. Hippocampal neurons 'entrapped' in the 3D collagen gel grew the same number, but shorter, processes and exhibited improved survival compared to neurons cultured in the 2D model. There was no difference in the electrophysiological properties of the neurons cultured in the 3D compared to the 2D model except in the resting membrane potential and in the duration of the after-hyperpolarization. Spontaneous postsynaptic currents were recorded in 14- and 21-day-old 3D cultures evidencing functional synapse formation. Our results indicate that the physiological characteristics of 3D neuronal cultures are similar to traditional 2D cultures. However, functional 3D networks of hippocampal neurons will be necessary for multi-level circuit formation, which could be essential for understanding the basis of physiological learning and memory. (c) 2009 Elsevier Ltd. All rights reserved.}, keywords = {Adult; IN-VITRO; MEMORY; NERVOUS-SYSTEM; GROWTH; NETWORKS; Electrophysiology; COLLAGEN; Three dimensional; NEURAL CELLS; SERUM-FREE CULTURE; RAT CORTICAL-NEURONS; 3-DIMENSIONAL EXTRACELLULAR-MATRIX; Neuronal network; Nerve tissue engineering; DEFINED SYSTEM}, year = {2009}, eissn = {1878-5905}, pages = {4377-4383}, orcid-numbers = {Molnár, Péter/0000-0002-1504-3092} } @article{MTMT:1789520, title = {Viability and electrophysiology of neural cell structures generated by the inkjet printing method}, url = {https://m2.mtmt.hu/api/publication/1789520}, author = {Xu, T and Gregory, CA and Molnár, Péter and Cui, X and Jalota, S and Bhaduri, SB and Boland, T}, doi = {10.1016/j.biomaterials.2006.01.048}, journal-iso = {BIOMATERIALS}, journal = {BIOMATERIALS}, volume = {27}, unique-id = {1789520}, issn = {0142-9612}, abstract = {Complex cellular patterns and structures were created by automated and direct inkjet printing of primary embryonic hippocampal and cortical neurons. Immunostaining analysis and whole-cell patch-clamp recordings showed that embryonic hippocampal and cortical neurons maintained basic cellular properties and functions, including normal, healthy neuronal phenotypes and electrophysiological characteristics, after being printed through thermal inkjet nozzles. In addition, in this study a new method was developed to create 3D cellular structures: sheets of neural cells were layered on each other (layer-by-layer process) by alternate inkjet printing of NT2 cells and fibrin gels. These results and findings, taken together, show that inkjet printing is rapidly evolving into a digital fabrication method to build functional neural structures that may eventually find applications in neural tissue engineering. (c) 2006 Published by Elsevier Ltd.}, keywords = {FIBRIN; HIPPOCAMPAL-NEURONS; INDUCTION; STRESS; thymus; CULTURE; COLLAGEN; FABRICATION; Tissue Engineering; CONSTRUCTS; 3D constructs; neural cell structure; inkjet printing; rat}, year = {2006}, eissn = {1878-5905}, pages = {3580-3588}, orcid-numbers = {Molnár, Péter/0000-0002-1504-3092} }