TY  - JOUR
AU  - Chandrasekaran, A
AU  - Avci, HX
AU  - Ochalek, A
AU  - Rosingh, LN
AU  - Molnár, Kinga
AU  - László, Lajos
AU  - Bellák, Tamás
AU  - Teglasi, A
AU  - Pesti, Krisztina
AU  - Mike, Árpád
AU  - Phanthong, P
AU  - Biró, Orsolya
AU  - Hall, V
AU  - Kitiyanant, N
AU  - Krause, KH
AU  - Kobolák, Julianna
AU  - Dinnyés, András
TI  - Comparison of 2D and 3D neural induction methods for the generation of neural progenitor cells from human induced pluripotent stem cells
JF  - STEM CELL RESEARCH
J2  - STEM CELL RES
VL  - 25
PY  - 2017
SP  - 139
EP  - 151
PG  - 13
SN  - 1873-5061
DO  - 10.1016/j.scr.2017.10.010
UR  - https://m2.mtmt.hu/api/publication/3312483
ID  - 3312483
N1  - BioTalentum Ltd, Gödöllő, Hungary            
            Department of Anatomy, Embryology and Histology, Faculty of Medicine, University of Szeged, Szeged, Hungary            
            Molecular Animal Biotechnology Lab, Szent István University, Gödöllő, Hungary            
            Department of Pathology and Immunology, University of Geneva Medical School, Geneva, Switzerland            
            Department of Anatomy, Cell and Developmental Biology, Eötvös Loránd University, Budapest, Hungary            
            Opto-Neuropharmacology Group, MTA-ELTE NAP B, Budapest, Hungary            
            János Szentágothai Doctoral School of Neurosciences, Semmelweis University, Budapest, Hungary            
            Stem Cell Research Group, Institute of Molecular Biosciences, Mahidol University, Nakhon Pathom Bangkok, Thailand            
            First Department of Obstetrics and Gynaecology, Semmelweis University, Budapest, Hungary            
            Department of Veterinary and Animal Science, University of Copenhagen, Denmark            
            Cited By :88            
            Export Date: 9 April 2024            
            Correspondence Address: Dinnyés, A.; BioTalentum LtdHungary; email: Manuscript.Dinnyes@biotalentum.hu
AB  - Neural progenitor cells (NPCs) from human induced pluripotent stem cells (hiPSCs) are frequently induced using 3D culture methodologies however, it is unknown whether spheroid-based (3D) neural induction is actually superior to monolayer (2D) neural induction. Our aim was to compare the efficiency of 2D induction with 3D induction method in their ability to generate NPCs, and subsequently neurons and astrocytes. Neural differentiation was analysed at the protein level qualitatively by immunocytochemistry and quantitatively by flow cytometry for NPC (SOX1, PAX6, NESTIN), neuronal (MAP2, TUBB3), cortical layer (TBR1, CUX1) and glial markers (SOX9, GFAP, AQP4). Electron microscopy demonstrated that both methods resulted in morphologically similar neural rosettes. However, quantification of NPCs derived from 3D neural induction exhibited an increase in the number of PAX6/NESTIN double positive cells and the derived neurons exhibited longer neurites. In contrast, 2D neural induction resulted in more SOX1 positive cells. While 2D monolayer induction resulted in slightly less mature neurons, at an early stage of differentiation, the patch clamp analysis failed to reveal any significant differences between the electrophysiological properties between the two induction methods. In conclusion, 3D neural induction increases the yield of PAX6(+)/NESTIN(+) cells and gives rise to neurons with longer neurites, which might be an advantage for the production of forebrain cortical neurons, highlighting the potential of 3D neural induction, independent of iPSCs' genetic background.
LA  - English
DB  - MTMT
ER  - 

TY  - JOUR
AU  - Nagy, József
AU  - Kobolák, Julianna
AU  - Berzsenyi, Sára
AU  - Ábrahám, Z
AU  - Avci, XH
AU  - Bock, István
AU  - Bekes, Z
AU  - Hodoscsek, B
AU  - Chandrasekaran, Abinaya
AU  - Téglási, A
AU  - Dezső, Péter
AU  - Koványi, Bence
AU  - T, Vörös E
AU  - Fodor, L
AU  - Szél, T
AU  - Németh, K
AU  - Balázs, A
AU  - Dinnyés, András
AU  - Lendvai, Balázs
AU  - Lévay, György István
AU  - Román, Viktor
TI  - Altered neurite morphology and cholinergic function of induced pluripotent stem cell-derived neurons from a patient with Kleefstra syndrome and autism
JF  - TRANSLATIONAL PSYCHIATRY
J2  - TRANSL PSYCHIAT
VL  - 7
PY  - 2017
IS  - 7
PG  - 10
SN  - 2158-3188
DO  - 10.1038/tp.2017.144
UR  - https://m2.mtmt.hu/api/publication/3253813
ID  - 3253813
N1  - Funding Agency and Grant Number: Gedeon Richter Plc.; Hungarian governmental grant [ERNYO-13-1-2013-000]; EU FP7 Marie Curie Fellowship (EpiHealthNet) [PITN-GA-2012-317146]; EU FP7 Marie Curie Fellowship (STEMMAD) [PIAPP-GA-2012-324451]
            Funding text: We are grateful to the donating healthy subjects and the Kleefstra patient for their participation in our study. The present study was financially supported by Gedeon Richter Plc. and a Hungarian governmental grant (ERNYO-13-1-2013-000). Abinaya Chandrasekaran and Hasan X. Avci were financially supported by an EU FP7 Marie Curie Fellowship (EpiHealthNet, PITN-GA-2012-317146; and STEMMAD, PIAPP-GA-2012-324451). We thank Kinga Molnar, Lajos Laszlo and Monika Truszka for their contribution in electron microscopy.

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LA  - English
DB  - MTMT
ER  -