@article{MTMT:3312483,
	title = {Comparison of 2D and 3D neural induction methods for the generation of neural progenitor cells from human induced pluripotent stem cells},
	url = {https://m2.mtmt.hu/api/publication/3312483},
	author = {Chandrasekaran, A and Avci, HX and Ochalek, A and Rosingh, LN and Molnár, Kinga and László, Lajos and Bellák, Tamás and Teglasi, A and Pesti, Krisztina and Mike, Árpád and Phanthong, P and Biró, Orsolya and Hall, V and Kitiyanant, N and Krause, KH and Kobolák, Julianna and Dinnyés, András},
	doi = {10.1016/j.scr.2017.10.010},
	journal-iso = {STEM CELL RES},
	journal = {STEM CELL RESEARCH},
	volume = {25},
	unique-id = {3312483},
	issn = {1873-5061},
	abstract = {Neural progenitor cells (NPCs) from human induced pluripotent stem cells (hiPSCs) are frequently induced using 3D culture methodologies however, it is unknown whether spheroid-based (3D) neural induction is actually superior to monolayer (2D) neural induction. Our aim was to compare the efficiency of 2D induction with 3D induction method in their ability to generate NPCs, and subsequently neurons and astrocytes. Neural differentiation was analysed at the protein level qualitatively by immunocytochemistry and quantitatively by flow cytometry for NPC (SOX1, PAX6, NESTIN), neuronal (MAP2, TUBB3), cortical layer (TBR1, CUX1) and glial markers (SOX9, GFAP, AQP4). Electron microscopy demonstrated that both methods resulted in morphologically similar neural rosettes. However, quantification of NPCs derived from 3D neural induction exhibited an increase in the number of PAX6/NESTIN double positive cells and the derived neurons exhibited longer neurites. In contrast, 2D neural induction resulted in more SOX1 positive cells. While 2D monolayer induction resulted in slightly less mature neurons, at an early stage of differentiation, the patch clamp analysis failed to reveal any significant differences between the electrophysiological properties between the two induction methods. In conclusion, 3D neural induction increases the yield of PAX6(+)/NESTIN(+) cells and gives rise to neurons with longer neurites, which might be an advantage for the production of forebrain cortical neurons, highlighting the potential of 3D neural induction, independent of iPSCs' genetic background.},
	year = {2017},
	eissn = {1876-7753},
	pages = {139-151},
	orcid-numbers = {Molnár, Kinga/0000-0002-7196-5331; László, Lajos/0000-0002-2114-9109; Mike, Árpád/0000-0002-9095-8161; Biró, Orsolya/0000-0002-4300-3602; Kobolák, Julianna/0000-0002-0986-9517}
}

@article{MTMT:3253813,
	title = {Altered neurite morphology and cholinergic function of induced pluripotent stem cell-derived neurons from a patient with Kleefstra syndrome and autism},
	url = {https://m2.mtmt.hu/api/publication/3253813},
	author = {Nagy, József and Kobolák, Julianna and Berzsenyi, Sára and Ábrahám, Z and Avci, XH and Bock, István and Bekes, Z and Hodoscsek, B and Chandrasekaran, Abinaya and Téglási, A and Dezső, Péter and Koványi, Bence and T, Vörös E and Fodor, L and Szél, T and Németh, K and Balázs, A and Dinnyés, András and Lendvai, Balázs and Lévay, György István and Román, Viktor},
	doi = {10.1038/tp.2017.144},
	journal-iso = {TRANSL PSYCHIAT},
	journal = {TRANSLATIONAL PSYCHIATRY},
	volume = {7},
	unique-id = {3253813},
	issn = {2158-3188},
	year = {2017},
	eissn = {2158-3188},
	orcid-numbers = {Kobolák, Julianna/0000-0002-0986-9517}
}

@article{MTMT:109936,
	title = {Excess of serotonin affects embryonic interneuron migration through activation of the serotonin receptor 6},
	url = {https://m2.mtmt.hu/api/publication/109936},
	author = {Riccio, O and Potter, G and Walzer, C and Vallet, P and Szabó, Gábor and Vutskits, L and Kiss, JZ and Dayer, AG},
	doi = {10.1038/mp.2008.89},
	journal-iso = {MOL PSYCHIATR},
	journal = {MOLECULAR PSYCHIATRY},
	volume = {14},
	unique-id = {109936},
	issn = {1359-4184},
	abstract = {The discovery that a common polymorphism (5-HTTLPR, short variant) in the human serotonin transporter gene (SLC6A4) can influence personality traits and increase the risk for depression in adulthood has led to the hypothesis that a relative increase in the extracellular levels of serotonin (5-HT) during development could be critical for the establishment of brain circuits. Consistent with this idea, a large body of data demonstrate that 5-HT is a strong neurodevelopmental signal that can modulate a wide variety of cellular processes. In humans, serotonergic fibers appear in the developing cortex as early as the 10th gestational week, a period of intense neuronal migration. In this study we hypothesized that an excess of 5-HT could affect embryonic cortical interneuron migration. Using time-lapse videometry to monitor the migration of interneurons in embryonic mouse cortical slices, we discovered that the application of 5-HT decreased interneuron migration in a reversible and dose-dependent manner. We next found that 5-HT6 receptors were expressed in cortical interneurons and that 5-HT6 receptor activation decreased interneuron migration, whereas 5-HT6 receptor blockade prevented the migratory effects induced by 5-HT. Finally, we observed that interneurons were abnormally distributed in the cerebral cortex of serotonin transporter gene (Slc6a4) knockout mice that have high levels of extracellular 5-HT. These results shed new light on the neurodevelopmental alterations caused by an excess of 5-HT during the embryonic period and contribute to a better understanding of the cellular processes that could be modulated by genetically controlled differences in human 5-HT homeostasis.Molecular Psychiatry advance online publication, 29 July 2008; doi:10.1038/mp.2008.89.},
	year = {2009},
	eissn = {1476-5578},
	pages = {280-290}
}