TY - JOUR AU - Vokalova, L. AU - Van, Breda S.V. AU - Ye, X.L. AU - Huhn, E.A. AU - Than, Nándor Gábor AU - Hasler, P. AU - Lapaire, O. AU - Hoesli, I. AU - Rossi, S.W. AU - Hahn, S. TI - Excessive neutrophil activity in gestational diabetes mellitus: Could it contribute to the development of preeclampsia? JF - FRONTIERS IN ENDOCRINOLOGY J2 - FRONT ENDOCRINOL VL - 9 ET - 0 PY - 2018 IS - SEP PG - 13 SN - 1664-2392 DO - 10.3389/fendo.2018.00542 UR - https://m2.mtmt.hu/api/publication/30322924 ID - 30322924 AB - Gestational diabetes mellitus is a transient form of glucose intolerance occurring during pregnancy. Pregnancies affected by gestational diabetes mellitus are at risk for the development of preeclampsia, a severe life threatening condition, associated with significant feto-maternal morbidity and mortality. It is a risk factor for long-term health in women and their offspring. Pregnancy has been shown to be associated with a subliminal degree of neutrophil activation and tightly regulated generation of neutrophil extracellular traps (NETs). This response is excessive in cases with preeclampsia, leading to the presence of large numbers of NETs in affected placentae. We have recently observed that circulatory neutrophils in cases with gestational diabetes mellitus similarly exhibit an excessive pro-NETotic phenotype, and pronounced placental presence, as detected by expression of neutrophil elastase. Furthermore, exogenous neutrophil elastase liberated by degranulating neutrophils was demonstrated to alter trophoblast physiology and glucose metabolism by interfering with key signal transduction components. In this review we examine whether additional evidence exists suggesting that altered neutrophil activity in gestational diabetes mellitus may contribute to the development of preeclampsia. © 2018 Vokalova, van Breda, Ye, Huhn, Than, Hasler, Lapaire, Hoesli, Rossi and Hahn. LA - English DB - MTMT ER - TY - JOUR AU - Biró, Orsolya AU - Alasztics, Bálint AU - Molvarec, Attila AU - Joó, József Gábor AU - Nagy, Bálint AU - Rigó, János TI - Various levels of circulating exosomal total-miRNA and miR-210 hypoxamiR in different forms of pregnancy hypertension JF - PREGNANCY HYPERTENSION J2 - PREGNANCY HYPERTENS VL - 10 PY - 2017 SP - 207 EP - 212 PG - 6 SN - 2210-7789 DO - 10.1016/j.preghy.2017.09.002 UR - https://m2.mtmt.hu/api/publication/3273020 ID - 3273020 AB - Introduction: Hypertension is a common complication during pregnancy, affecting 10% of pregnant women worldwide. Several microRNA (miRNA) were shown to be involved in hypertensive disorders of pregnancy. In preeclampsia (PE), placental dysfunction causes the enhanced release of extracellular vesicle-derived miRNAs. The hypoxia-sensitive hsa-mir-210 is the most common PE-associated miRNA, but its exosomal profile has not been investigated. Objectives: Our aims were to measure exosomal total-miRNA concentration and to perform expression analysis of circulating exosomal hsa-miR-210 in women affected by chronic hypertension (CHT) gestational hypertension (GHT) or PE. Materials and methods: We collected plasma samples from women with CHT, GHT, PE (moderate: mPE and severe: sPE) and from normotensive pregnancies. Exosomal miRNAs were extracted and miRNA concentration was measured. RT-PCR was carried out with hsa-miR-210-3p-specific primers and relative expression was calculated using the comparative Ct method. Results: The total-miRNA concentration was different in the disease subgroups, and was significantly higher in mPE and sPE compared to the other groups. We found a significant difference in the relative exosomal hsa-miR-210-3p expression between all hypertensive groups compared to the normotensive samples, but significant upregulation was only observed in case of mPE and sPE patients. Both the level of total-miRNA and hsa-miR-210 expression was higher in case of severe PE. Conclusions: The level of circulating exosomal total-miRNA and hsa-miR-210 was elevated in women with PE, and it was higher in the severe form. We showed that hsa-miR-210 is secreted via exosomes, which may have a role in the pathomechanism of the disease. © 2017 International Society for the Study of Hypertension in Pregnancy. LA - English DB - MTMT ER - TY - JOUR AU - Németh, Andrea AU - Orgován, Norbert AU - Sódar, Barbara AU - Osteikoetxea, Xabier AU - Pálóczi, Krisztina AU - Szabó-Taylor, Katalin AU - Visnovitzné Dr Vukman, Krisztina AU - Kittel, Ágnes AU - Turiák, Lilla AU - Wiener, Zoltán AU - Tóth, Sára AU - Drahos, László AU - Vékey, Károly AU - Horváth, Róbert AU - Buzás, Edit Irén TI - Antibiotic-induced release of small extracellular vesicles (exosomes) with surface-associated DNA JF - SCIENTIFIC REPORTS J2 - SCI REP VL - 7 PY - 2017 PG - 16 SN - 2045-2322 DO - 10.1038/s41598-017-08392-1 UR - https://m2.mtmt.hu/api/publication/3254659 ID - 3254659 LA - English DB - MTMT ER - TY - JOUR AU - Baranyai, Tamás AU - Herczeg, Kata AU - Onódi, Zsófia AU - Voszka, István AU - Módos, Károly AU - Marton, Nikolett AU - Nagy, György AU - Mager, I AU - Wood, MJ AU - El, Andaloussi S AU - Pálinkás, Zoltán AU - Kumar, V AU - Nagy, Péter AU - Kittel, Ágnes AU - Buzás, Edit Irén AU - Ferdinandy, Péter AU - Giricz, Zoltán TI - Isolation of Exosomes from Blood Plasma: Qualitative and Quantitative Comparison of Ultracentrifugation and Size Exclusion Chromatography Methods JF - PLOS ONE J2 - PLOS ONE VL - 10 PY - 2015 IS - 12 PG - 13 SN - 1932-6203 DO - 10.1371/journal.pone.0145686 UR - https://m2.mtmt.hu/api/publication/2987507 ID - 2987507 N1 - Department of Pharmacology and Pharmacotherapy, Semmelweis University, Budapest, Hungary Department of Biophysics and Radiation Biology, Semmelweis University, Budapest, Hungary Department of Genetics, Cell- and Immunobiology, Semmelweis University, Budapest, Hungary Department of Rheumatology, Polyclinic of the Hospitaller Brothers of St John of God, Budapest, Hungary Department of Physiology, Anatomy and Genetics, University of Oxford, Oxford, United Kingdom Institute of Technology, University of Tartu, Tartu, Estonia Department of Laboratory Medicine, Karolinska Institutet, Stockholm, Sweden Department of Molecular Immunology and Toxicology, National Institute of Oncology, Budapest, Hungary Centre for Cellular and Molecular Platforms, NCBS, Bangalore, India Department of Pharmacology, Institute of Experimental Medicine, Hungarian Academy of Sciences, Budapest, Hungary Pharmahungary Group, Budapest, Hungary Cited By :407 Export Date: 13 June 2023 CODEN: POLNC Correspondence Address: Giricz, Z.; Department of Pharmacology and Pharmacotherapy, Hungary; email: giricz.zoltan@med.semmelweis-univ.hu Funding details: PITN-GA-2011-289033 Funding details: Seventh Framework Programme, FP7, 289033 Funding details: European Cooperation in Science and Technology, COST Funding details: Eesti Teadusagentuur, ETAg, PUT618 Funding details: Hungarian Scientific Research Fund, OTKA, OTKA K 109843, OTKA NK 111958, OTKA NK 84043, PD 109051 Funding text 1: This work was supported by the Hungarian Scientific Research Fund (OTKA PD 109051, OTKA NK 84043, OTKA NK 111958, OTKA K 109843) and the following grants: Marie Curie Networks for Initial Training-ITN-FP7-PEOPLE-2011-ITN, PITN-GA-2011-289033 and COST Action BM1202 ME-HAD. P.N. and Z.G. hold a “János Bolyai Fellowship” from the Hungarian Academy of Sciences. I.M. is supported by the Estonian Research Council Grant PUT618. AB - BACKGROUND: Exosomes are emerging targets for biomedical research. However, suitable methods for the isolation of blood plasma-derived exosomes without impurities have not yet been described. AIM: Therefore, we investigated the efficiency and purity of exosomes isolated with potentially suitable methods; differential ultracentrifugation (UC) and size exclusion chromatography (SEC). METHODS AND RESULTS: Exosomes were isolated from rat and human blood plasma by various UC and SEC conditions. Efficiency was investigated at serial UC of the supernatant, while in case of SEC by comparing the content of exosomal markers of various fractions. Purity was assessed based on the presence of albumin. We found that the diameter of the majority of isolated particles fell into the size range of exosomes, however, albumin was also present in the preparations, when 1h UC at 4 degrees C was applied. Furthermore, with this method only a minor fraction of total exosomes could be isolated from blood as deduced from the constant amount of exosomal markers CD63 and TSG101 detected after serial UC of rat blood plasma samples. By using UC for longer time or with shorter sedimentation distance at 4 degrees C, or UC performed at 37 degrees C, exosomal yield increased, but albumin impurity was still observed in the isolates, as assessed by transmission electron microscopy, dynamic light scattering and immunoblotting against CD63, TSG101 and albumin. Efficiency and purity were not different in case of using further diluted samples. By using SEC with different columns, we have found that although a minor fraction of exosomes can be isolated without significant albumin content on Sepharose CL-4B or Sephacryl S-400 columns, but not on Sepharose 2B columns, the majority of exosomes co-eluted with albumin. CONCLUSION: Here we show that it is feasible to isolate exosomes from blood plasma by SEC without significant albumin contamination albeit with low vesicle yield. LA - English DB - MTMT ER - TY - JOUR AU - Yanez-Mo, M AU - Siljander, PR AU - Andreu, Z AU - Zavec, AB AU - Borras, FE AU - Buzás, Edit Irén AU - Buzás, Krisztina AU - Casal, E AU - Cappello, F AU - Carvalho, J AU - Colas, E AU - Cordeiro-da Silva, A AU - Fais, S AU - Falcon-Perez, JM AU - Ghobrial, IM AU - Giebel, B AU - Gimona, M AU - Graner, M AU - Gursel, I AU - Gursel, M AU - Heegaard, NH AU - Hendrix, A AU - Kierulf, P AU - Kokubun, K AU - Kosanovic, M AU - Kralj-Iglic, V AU - Kramer-Albers, EM AU - Laitinen, S AU - Lasser, C AU - Lener, T AU - Ligeti, Erzsébet AU - Line, A AU - Lipps, G AU - Llorente, A AU - Lotvall, J AU - Mancek-Keber, M AU - Marcilla, A AU - Mittelbrunn, M AU - Nazarenko, I AU - Nolte-'t, Hoen EN AU - Nyman, TA AU - O'Driscoll, L AU - Olivan, M AU - Oliveira, C AU - Pállinger, Éva AU - Del Portillo, HA AU - Reventos, J AU - Rigau, M AU - Rohde, E AU - Sammar, M AU - Sanchez-Madrid, F AU - Santarem, N AU - Schallmoser, K AU - Ostenfeld, MS AU - Stoorvogel, W AU - Stukelj, R AU - Van, der Grein SG AU - Vasconcelos, MH AU - Wauben, MH AU - De Wever, O TI - Biological properties of extracellular vesicles and their physiological functions JF - JOURNAL OF EXTRACELLULAR VESICLES J2 - J EXTRACELLULAR VESICL VL - 4 PY - 2015 PG - 60 SN - 2001-3078 DO - 10.3402/jev.v4.27066 UR - https://m2.mtmt.hu/api/publication/2930099 ID - 2930099 N1 - Yanez-Mo M and Siljander PR authors have contributed equally. The rest of the authors are listed in alphabetical order. Unidad de Investigación, Hospital Sta Cristina, Instituto de Investigaciones Sanitarias Princesa (IIS-IP), Madrid, Spain Departamento de Biología Molecular, UAM, Madrid, Spain Extracellular Vesicle Research, Division of Biochemistry and Biotechnology, Department of Biosciences, University of Helsinki, Helsinki, Finland Division of Pharmaceutical Biosciences, Faculty of Pharmacy, University of Helsinki, Helsinki, Finland Laboratory for Molecular Biology and Nanobiotechnology, National Institute of Chemistry, Ljubljana, Slovenia IVECAT Group, 'Germans Trias i Pujol' Research Institute, Badalona, Spain Nephrology Service, 'Germans Trias i Pujol' University Hospital, Badalona, Spain Department of Genetics, Cell- and Immunobiology, Semmelweis University, Budapest, Hungary Biological Research Centre, Hungarian Academy of Sciences, Szeged, Hungary Faculty of Dentistry, University of Szeged, Szeged, Hungary Metabolomics Unit, CIC bioGUNE, CIBERehd, Bizkaia Technology Park, Derio, Spain Department of Experimental Biomedicine and Clinical Neuroscience, Human Anatomy Section, University of Palermo, Palermo, Italy Euro-Mediterranean Institute of Science and Technology, Palermo, Italy Instituto de Investigação e Inovação em Saú de, Universidade do Porto, Porto, Portugal Expression Regulation in Cancer, Institute of Molecular Pathology and Immunology of the University of Porto (IPATIMUP), Porto, Portugal Research Unit in Biomedicine and Translational Oncology, Vall Hebron Institute of Research and Autonomous, University of Barcelona, Barcelona, Spain IBMC, Instituto de Biologia Molecular e Celular, Universidade do Porto, Porto, Portugal Department of Biological Sciences, Faculty of Pharmacy, University of Porto, Porto, Portugal Anti-Tumour Drugs Section, Department of Therapeutic Research and Medicines Evaluation, National Institute of Health (ISS), Rome, Italy IKERBASQUE, Basque Foundation for Science, Bilbao, Spain Dana-Farber Cancer Institute, Boston, MA, United States Institute for Transfusion Medicine, University Hospital Essen, University of Duisburg-Essen, Essen, Germany Spinal Cord Injury and Tissue Regeneration Center Salzburg (SCI-TReCS), Paracelsus Medical University (PMU), Salzburg, Austria Department of Blood Group Serology and Transfusion Medicine, Universitätsklinikum, Salzburger Landeskliniken GesmbH (SALK), Salzburg, Austria Department of Neurosurgery, University of Colorado DenverCO, United States Department of Molecular Biology and Genetics, Thorlab-Therapeutic Oligonucleotide Research Lab, Bilkent University, Ankara, Turkey Department of Biological Sciences, Middle East Technical University, Ankara, Turkey Department of Clinical Biochemistry and Pharmacology, Odense University Hospital, University of Southern Denmark, Odense, Denmark Analytical Protein Chemistry, Department of Clinical Biochemistry, Immunology and Genetics, Statens Serum Institut, Copenhagen, Denmark Laboratory of Experimental Cancer Research, Department of Radiation Oncology and Experimental Cancer Research, Ghent University Hospital, Ghent, Belgium Bood Cell Research Group, Department of Medical Biochemistry, Oslo University Hospital, Oslo, Norway Department of Immunochemistry and Glycobiology, Institute for the Application of Nuclear Energy, INEP, University of Belgrade, Belgrade, Serbia Laboratory of Clinical Biophysics, Faculty of Health Sciences, University of Ljubljana, Ljubljana, Slovenia Molecular Cell Biology and Focus Program Translational Neurosciences, University of Mainz, Mainz, Germany Research and Cell Services, Finnish Red Cross Blood Service, Helsinki, Finland Krefting Research Centre, Institute of Medicine at the Sahlgrenska Academy, University of Gothenburg, Gothenburg, Sweden Department of Physiology, Semmelweis University, Budapest, Hungary Latvian Biomedical Research and Study Centre, Riga, Latvia Institute of Chemistry and Bioanalytics, School of Life Sciences, University of Applied Sciences and Arts Northwestern Switzerland, Muttenz, Switzerland Department of Molecular Cell Biology, Institute for Cancer Research, Oslo University Hospital - The Norwegian Radium Hospital, Oslo, Norway National Institute of Chemistry, Laboratory of Biotechnology, Ljubljana, Slovenia ENFIST Centre of Excellence, Ljubljana, Slovenia Departamento de Biología Celular y Parasitologia, Facultat de Farmacia, Universitat de Valencia, Valencia, Spain Department of Vascular Biology and Inflammation, Centro Nacional de Investigaciones Cardiovasculares, Madrid, Spain Institute for Environmental Health Sciences, Hospital Infection Control Medical Center, University of Freiburg, Freiburg im Breisgau, Germany Department of Biochemistry and Cell Biology, Faculty of Veterinary Medicine, Utrecht University, Utrecht, Netherlands Institute of Biotechnology, University of Helsinki, Viikinkaari 1, Helsinki, Finland School of Pharmacy and Pharmaceutical Sciences, Trinity Biomedical Sciences Institute, Trinity College Dublin, Dublin, Ireland Department of Pathology and Oncology, Faculty of Medicine, University of Porto, Porto, Portugal ISGlobal, Barcelona Ctr. Int. Health Res. (CRESIB), Hospital Clínic - Universitat de Barcelona, Barcelona, Spain Institució Catalana de Recerca I Estudis Avançats, Barcelona, Spain Departament de Ciències Bàsiques, Universitat Internacional de Catalunya, Institut de Recerca Biomèdica de Bellvitge, Barcelona, Spain Department of Biotechnology Engineering, ORT Braude College, Karmiel, Israel Servicio de Inmunología, Hospital de la Princesa, Instituto de Investigaciones Sanitarias Princesa (IIS-IP), Madrid, Spain Departmnet of Molecular Medicine, Aarhus University Hospital, Aarhus, Denmark Cancer Drug Resistance Group, Institute of Molecular Pathology and Immunology, University of Porto (IPATIMUP), Porto, Portugal Department of Biological Sciences, Faculty of Pharmacy, University of Porto (FFUP), Porto, Portugal Cited By :955 Export Date: 30 August 2019 Correspondence Address: Yáñez-Mó, M.; Membrane Microdomains in Immunity Laboratory, Unidad de Investigación, Hospital Santa Cristina, Instituto de Investigación Sanitaria Princesa, Departamento de Biología Molecular, UAM, C/Maestro Amadeo Vives 2, edificio consultas 5a planta, Spain Chemicals/CAS: DNA, 9007-49-2; lipid, 66455-18-3; RNA, 63231-63-0 Unidad de Investigación, Hospital Sta Cristina, Instituto de Investigaciones Sanitarias Princesa (IIS-IP), Madrid, Spain Departamento de Biología Molecular, UAM, Madrid, Spain Extracellular Vesicle Research, Division of Biochemistry and Biotechnology, Department of Biosciences, University of Helsinki, Helsinki, Finland Division of Pharmaceutical Biosciences, Faculty of Pharmacy, University of Helsinki, Helsinki, Finland Laboratory for Molecular Biology and Nanobiotechnology, National Institute of Chemistry, Ljubljana, Slovenia IVECAT Group, 'Germans Trias i Pujol' Research Institute, Badalona, Spain Nephrology Service, 'Germans Trias i Pujol' University Hospital, Badalona, Spain Department of Genetics, Cell- and Immunobiology, Semmelweis University, Budapest, Hungary Biological Research Centre, Hungarian Academy of Sciences, Szeged, Hungary Faculty of Dentistry, University of Szeged, Szeged, Hungary Metabolomics Unit, CIC bioGUNE, CIBERehd, Bizkaia Technology Park, Derio, Spain Department of Experimental Biomedicine and Clinical Neuroscience, Human Anatomy Section, University of Palermo, Palermo, Italy Euro-Mediterranean Institute of Science and Technology, Palermo, Italy Instituto de Investigação e Inovação em Saú de, Universidade do Porto, Porto, Portugal Expression Regulation in Cancer, Institute of Molecular Pathology and Immunology of the University of Porto (IPATIMUP), Porto, Portugal Research Unit in Biomedicine and Translational Oncology, Vall Hebron Institute of Research and Autonomous, University of Barcelona, Barcelona, Spain IBMC, Instituto de Biologia Molecular e Celular, Universidade do Porto, Porto, Portugal Department of Biological Sciences, Faculty of Pharmacy, University of Porto, Porto, Portugal Anti-Tumour Drugs Section, Department of Therapeutic Research and Medicines Evaluation, National Institute of Health (ISS), Rome, Italy IKERBASQUE, Basque Foundation for Science, Bilbao, Spain Dana-Farber Cancer Institute, Boston, MA, United States Institute for Transfusion Medicine, University Hospital Essen, University of Duisburg-Essen, Essen, Germany Spinal Cord Injury and Tissue Regeneration Center Salzburg (SCI-TReCS), Paracelsus Medical University (PMU), Salzburg, Austria Department of Blood Group Serology and Transfusion Medicine, Universitätsklinikum, Salzburger Landeskliniken GesmbH (SALK), Salzburg, Austria Department of Neurosurgery, University of Colorado DenverCO, United States Department of Molecular Biology and Genetics, Thorlab-Therapeutic Oligonucleotide Research Lab, Bilkent University, Ankara, Turkey Department of Biological Sciences, Middle East Technical University, Ankara, Turkey Department of Clinical Biochemistry and Pharmacology, Odense University Hospital, University of Southern Denmark, Odense, Denmark Analytical Protein Chemistry, Department of Clinical Biochemistry, Immunology and Genetics, Statens Serum Institut, Copenhagen, Denmark Laboratory of Experimental Cancer Research, Department of Radiation Oncology and Experimental Cancer Research, Ghent University Hospital, Ghent, Belgium Bood Cell Research Group, Department of Medical Biochemistry, Oslo University Hospital, Oslo, Norway Department of Immunochemistry and Glycobiology, Institute for the Application of Nuclear Energy, INEP, University of Belgrade, Belgrade, Serbia Laboratory of Clinical Biophysics, Faculty of Health Sciences, University of Ljubljana, Ljubljana, Slovenia Molecular Cell Biology and Focus Program Translational Neurosciences, University of Mainz, Mainz, Germany Research and Cell Services, Finnish Red Cross Blood Service, Helsinki, Finland Krefting Research Centre, Institute of Medicine at the Sahlgrenska Academy, University of Gothenburg, Gothenburg, Sweden Department of Physiology, Semmelweis University, Budapest, Hungary Latvian Biomedical Research and Study Centre, Riga, Latvia Institute of Chemistry and Bioanalytics, School of Life Sciences, University of Applied Sciences and Arts Northwestern Switzerland, Muttenz, Switzerland Department of Molecular Cell Biology, Institute for Cancer Research, Oslo University Hospital - The Norwegian Radium Hospital, Oslo, Norway National Institute of Chemistry, Laboratory of Biotechnology, Ljubljana, Slovenia ENFIST Centre of Excellence, Ljubljana, Slovenia Departamento de Biología Celular y Parasitologia, Facultat de Farmacia, Universitat de Valencia, Valencia, Spain Department of Vascular Biology and Inflammation, Centro Nacional de Investigaciones Cardiovasculares, Madrid, Spain Institute for Environmental Health Sciences, Hospital Infection Control Medical Center, University of Freiburg, Freiburg im Breisgau, Germany Department of Biochemistry and Cell Biology, Faculty of Veterinary Medicine, Utrecht University, Utrecht, Netherlands Institute of Biotechnology, University of Helsinki, Viikinkaari 1, Helsinki, Finland School of Pharmacy and Pharmaceutical Sciences, Trinity Biomedical Sciences Institute, Trinity College Dublin, Dublin, Ireland Department of Pathology and Oncology, Faculty of Medicine, University of Porto, Porto, Portugal ISGlobal, Barcelona Ctr. Int. Health Res. (CRESIB), Hospital Clínic - Universitat de Barcelona, Barcelona, Spain Institució Catalana de Recerca I Estudis Avançats, Barcelona, Spain Departament de Ciències Bàsiques, Universitat Internacional de Catalunya, Institut de Recerca Biomèdica de Bellvitge, Barcelona, Spain Department of Biotechnology Engineering, ORT Braude College, Karmiel, Israel Servicio de Inmunología, Hospital de la Princesa, Instituto de Investigaciones Sanitarias Princesa (IIS-IP), Madrid, Spain Departmnet of Molecular Medicine, Aarhus University Hospital, Aarhus, Denmark Cancer Drug Resistance Group, Institute of Molecular Pathology and Immunology, University of Porto (IPATIMUP), Porto, Portugal Department of Biological Sciences, Faculty of Pharmacy, University of Porto (FFUP), Porto, Portugal Cited By :1100 Export Date: 14 January 2020 Correspondence Address: Yáñez-Mó, M.; Membrane Microdomains in Immunity Laboratory, Unidad de Investigación, Hospital Santa Cristina, Instituto de Investigación Sanitaria Princesa, Departamento de Biología Molecular, UAM, C/Maestro Amadeo Vives 2, edificio consultas 5a planta, Spain Chemicals/CAS: DNA, 9007-49-2; lipid, 66455-18-3; RNA, 63231-63-0 Funding Agency and Grant Number: European Network on Microvesicles and Exosomes in Health & Disease (ME-HaD) [BM1202]; ICREAICREA Funding text: The authors wish to thank Dr R Simpson and Dr D Taylor for critical reading of the manuscript and acknowledge the Horizon 2020 European Cooperation in Science and Technology programme and its support of our European Network on Microvesicles and Exosomes in Health & Disease (ME-HaD; BM1202 www.cost.eu/COST_Actions/bmbs/Actions/BM1202) Unidad de Investigación, Hospital Sta Cristina, Instituto de Investigaciones Sanitarias Princesa (IIS-IP), Madrid, Spain Departamento de Biología Molecular, UAM, Madrid, Spain Extracellular Vesicle Research, Division of Biochemistry and Biotechnology, Department of Biosciences, University of Helsinki, Helsinki, Finland Division of Pharmaceutical Biosciences, Faculty of Pharmacy, University of Helsinki, Helsinki, Finland Laboratory for Molecular Biology and Nanobiotechnology, National Institute of Chemistry, Ljubljana, Slovenia IVECAT Group, 'Germans Trias i Pujol' Research Institute, Badalona, Spain Nephrology Service, 'Germans Trias i Pujol' University Hospital, Badalona, Spain Department of Genetics, Cell- and Immunobiology, Semmelweis University, Budapest, Hungary Biological Research Centre, Hungarian Academy of Sciences, Szeged, Hungary Faculty of Dentistry, University of Szeged, Szeged, Hungary Metabolomics Unit, CIC bioGUNE, CIBERehd, Bizkaia Technology Park, Derio, Spain Department of Experimental Biomedicine and Clinical Neuroscience, Human Anatomy Section, University of Palermo, Palermo, Italy Euro-Mediterranean Institute of Science and Technology, Palermo, Italy Instituto de Investigação e Inovação em Saú de, Universidade do Porto, Porto, Portugal Expression Regulation in Cancer, Institute of Molecular Pathology and Immunology of the University of Porto (IPATIMUP), Porto, Portugal Research Unit in Biomedicine and Translational Oncology, Vall Hebron Institute of Research and Autonomous, University of Barcelona, Barcelona, Spain IBMC, Instituto de Biologia Molecular e Celular, Universidade do Porto, Porto, Portugal Department of Biological Sciences, Faculty of Pharmacy, University of Porto, Porto, Portugal Anti-Tumour Drugs Section, Department of Therapeutic Research and Medicines Evaluation, National Institute of Health (ISS), Rome, Italy IKERBASQUE, Basque Foundation for Science, Bilbao, Spain Dana-Farber Cancer Institute, Boston, MA, United States Institute for Transfusion Medicine, University Hospital Essen, University of Duisburg-Essen, Essen, Germany Spinal Cord Injury and Tissue Regeneration Center Salzburg (SCI-TReCS), Paracelsus Medical University (PMU), Salzburg, Austria Department of Blood Group Serology and Transfusion Medicine, Universitätsklinikum, Salzburger Landeskliniken GesmbH (SALK), Salzburg, Austria Department of Neurosurgery, University of Colorado DenverCO, United States Department of Molecular Biology and Genetics, Thorlab-Therapeutic Oligonucleotide Research Lab, Bilkent University, Ankara, Turkey Department of Biological Sciences, Middle East Technical University, Ankara, Turkey Department of Clinical Biochemistry and Pharmacology, Odense University Hospital, University of Southern Denmark, Odense, Denmark Analytical Protein Chemistry, Department of Clinical Biochemistry, Immunology and Genetics, Statens Serum Institut, Copenhagen, Denmark Laboratory of Experimental Cancer Research, Department of Radiation Oncology and Experimental Cancer Research, Ghent University Hospital, Ghent, Belgium Bood Cell Research Group, Department of Medical Biochemistry, Oslo University Hospital, Oslo, Norway Department of Immunochemistry and Glycobiology, Institute for the Application of Nuclear Energy, INEP, University of Belgrade, Belgrade, Serbia Laboratory of Clinical Biophysics, Faculty of Health Sciences, University of Ljubljana, Ljubljana, Slovenia Molecular Cell Biology and Focus Program Translational Neurosciences, University of Mainz, Mainz, Germany Research and Cell Services, Finnish Red Cross Blood Service, Helsinki, Finland Krefting Research Centre, Institute of Medicine at the Sahlgrenska Academy, University of Gothenburg, Gothenburg, Sweden Department of Physiology, Semmelweis University, Budapest, Hungary Latvian Biomedical Research and Study Centre, Riga, Latvia Institute of Chemistry and Bioanalytics, School of Life Sciences, University of Applied Sciences and Arts Northwestern Switzerland, Muttenz, Switzerland Department of Molecular Cell Biology, Institute for Cancer Research, Oslo University Hospital - The Norwegian Radium Hospital, Oslo, Norway National Institute of Chemistry, Laboratory of Biotechnology, Ljubljana, Slovenia ENFIST Centre of Excellence, Ljubljana, Slovenia Departamento de Biología Celular y Parasitologia, Facultat de Farmacia, Universitat de Valencia, Valencia, Spain Department of Vascular Biology and Inflammation, Centro Nacional de Investigaciones Cardiovasculares, Madrid, Spain Institute for Environmental Health Sciences, Hospital Infection Control Medical Center, University of Freiburg, Freiburg im Breisgau, Germany Department of Biochemistry and Cell Biology, Faculty of Veterinary Medicine, Utrecht University, Utrecht, Netherlands Institute of Biotechnology, University of Helsinki, Viikinkaari 1, Helsinki, Finland School of Pharmacy and Pharmaceutical Sciences, Trinity Biomedical Sciences Institute, Trinity College Dublin, Dublin, Ireland Department of Pathology and Oncology, Faculty of Medicine, University of Porto, Porto, Portugal ISGlobal, Barcelona Ctr. Int. Health Res. (CRESIB), Hospital Clínic - Universitat de Barcelona, Barcelona, Spain Institució Catalana de Recerca I Estudis Avançats, Barcelona, Spain Departament de Ciències Bàsiques, Universitat Internacional de Catalunya, Institut de Recerca Biomèdica de Bellvitge, Barcelona, Spain Department of Biotechnology Engineering, ORT Braude College, Karmiel, Israel Servicio de Inmunología, Hospital de la Princesa, Instituto de Investigaciones Sanitarias Princesa (IIS-IP), Madrid, Spain Departmnet of Molecular Medicine, Aarhus University Hospital, Aarhus, Denmark Cancer Drug Resistance Group, Institute of Molecular Pathology and Immunology, University of Porto (IPATIMUP), Porto, Portugal Department of Biological Sciences, Faculty of Pharmacy, University of Porto (FFUP), Porto, Portugal Cited By :1581 Export Date: 13 January 2021 Correspondence Address: Yáñez-Mó, M.; Membrane Microdomains in Immunity Laboratory, Unidad de Investigación, Hospital Santa Cristina, Instituto de Investigación Sanitaria Princesa, Departamento de Biología Molecular, UAM, C/Maestro Amadeo Vives 2, edificio consultas 5a planta, Spain Chemicals/CAS: DNA, 9007-49-2; lipid, 66455-18-3; RNA, 63231-63-0 Funding Agency and Grant Number: European Network on Microvesicles and Exosomes in Health & Disease (ME-HaD) [BM1202]; ICREAICREA Funding Source: Custom Funding text: The authors wish to thank Dr R Simpson and Dr D Taylor for critical reading of the manuscript and acknowledge the Horizon 2020 European Cooperation in Science and Technology programme and its support of our European Network on Microvesicles and Exosomes in Health & Disease (ME-HaD; BM1202 www.cost.eu/COST_Actions/bmbs/Actions/BM1202) AB - In the past decade, extracellular vesicles (EVs) have been recognized as potent vehicles of intercellular communication, both in prokaryotes and eukaryotes. This is due to their capacity to transfer proteins, lipids and nucleic acids, thereby influencing various physiological and pathological functions of both recipient and parent cells. While intensive investigation has targeted the role of EVs in different pathological processes, for example, in cancer and autoimmune diseases, the EV-mediated maintenance of homeostasis and the regulation of physiological functions have remained less explored. Here, we provide a comprehensive overview of the current understanding of the physiological roles of EVs, which has been written by crowd-sourcing, drawing on the unique EV expertise of academia-based scientists, clinicians and industry based in 27 European countries, the United States and Australia. This review is intended to be of relevance to both researchers already working on EV biology and to newcomers who will encounter this universal cell biological system. Therefore, here we address the molecular contents and functions of EVs in various tissues and body fluids from cell systems to organs. We also review the physiological mechanisms of EVs in bacteria, lower eukaryotes and plants to highlight the functional uniformity of this emerging communication system. LA - English DB - MTMT ER -