TY - JOUR AU - Vizsnyiczai, Gaszton AU - Frangipane, G AU - Maggi, C AU - Saglimbeni, F AU - Bianchi, S AU - Di Leonardo, R TI - Light controlled 3D micromotors powered by bacteria JF - NATURE COMMUNICATIONS J2 - NAT COMMUN VL - 8 PY - 2017 SN - 2041-1723 DO - 10.1038/ncomms15974 UR - https://m2.mtmt.hu/api/publication/3289900 ID - 3289900 AB - Self-propelled bacteria can be integrated into synthetic micromachines and act as biological propellers. So far, proposed designs suffer from low reproducibility, large noise levels or lack of tunability. Here we demonstrate that fast, reliable and tunable bio-hybrid micromotors can be obtained by the self-assembly of synthetic structures with genetically engineered biological propellers. The synthetic components consist of 3D interconnected structures having a rotating unit that can capture individual bacteria into an array of microchambers so that cells contribute maximally to the applied torque. Bacterial cells are smooth swimmers expressing a light-driven proton pump that allows to optically control their swimming speed. Using a spatial light modulator, we can address individual motors with tunable light intensities allowing the dynamic control of their rotational speeds. Applying a real-time feedback control loop, we can also command a set of micromotors to rotate in unison with a prescribed angular speed. LA - English DB - MTMT ER - TY - JOUR AU - Aekbote Lakshman Rao, Badri AU - Fekete, Tamás AU - Jacak, J AU - Vizsnyiczai, Gaszton AU - Ormos, Pál AU - Kelemen, Lóránd TI - Surface-modified complex SU-8 microstructures for indirect optical manipulation of single cells JF - BIOMEDICAL OPTICS EXPRESS J2 - BIOMED OPT EXPRESS VL - 7 PY - 2016 IS - 1 SP - 45 EP - 56 PG - 12 SN - 2156-7085 DO - 10.1364/BOE.7.000045 UR - https://m2.mtmt.hu/api/publication/3027725 ID - 3027725 N1 - Biological Research Centre of the Hungarian Academy of Sciences, Temesvári krt. 62, Szeged, 6726, Hungary University of Applied Sciences Upper Austria, Garnisonstraße 21, Linz, 4020, Austria Cited By :12 Export Date: 4 February 2020 Biological Research Centre of the Hungarian Academy of Sciences, Temesvári krt. 62, Szeged, 6726, Hungary University of Applied Sciences Upper Austria, Garnisonstraße 21, Linz, 4020, Austria Cited By :19 Export Date: 27 April 2021 AB - We introduce a method that combines two-photon polymerization (TPP) and surface functionalization to enable the indirect optical manipulation of live cells. TPP-made 3D microstructures were coated specifically with a multilayer of the protein streptavidin and non-specifically with IgG antibody using polyethylene glycol diamine as a linker molecule. Protein density on their surfaces was quantified for various coating methods. The streptavidin-coated structures were shown to attach to biotinated cells reproducibly. We performed basic indirect optical micromanipulation tasks with attached structure-cell couples using complex structures and a multi-focus optical trap. The use of such extended manipulators for indirect optical trapping ensures to keep a safe distance between the trapping beams and the sensitive cell and enables their 6 degrees of freedom actuation. (C)2015 Optical Society of America LA - English DB - MTMT ER - TY - JOUR AU - Vizsnyiczai, Gaszton AU - Aekbote Lakshman Rao, Badri AU - Búzás, András AU - Grexa, István AU - Ormos, Pál AU - Kelemen, Lóránd TI - High accuracy indirect optical manipulation of live cells with functionalized microtools JF - PROCEEDINGS OF SPIE - THE INTERNATIONAL SOCIETY FOR OPTICAL ENGINEERING J2 - PROCEEDINGS OF SPIE VL - 9922 PY - 2016 IS - San Diego PG - 7 SN - 0277-786X DO - 10.1117/12.2237601 UR - https://m2.mtmt.hu/api/publication/3120643 ID - 3120643 AB - Optical micro manipulation of live cells has been extensively used to study a wide range of cellular phenomena with relevance in basic research or in diagnostics. The approaches span from manipulation of many cells for high throughput measurement or sorting, to more elaborated studies of intracellular events on trapped single cells when coupled with modern imaging techniques. In case of direct cell trapping the damaging effects of light-cell interaction must be minimized, for instance with the choice of proper laser wavelength. Microbeads have already been used for trapping cells indirectly thereby reducing the irradiation damage and increasing trapping efficiency with their high refractive index contrast. We show here that such intermediate objects can be tailor-made for indirect cell trapping to further increase cell-to-focal spot distance while maintaining their free and fast maneuverability. Carefully designed structures were produced with two-photon polymerization with shapes optimized for effective manipulation and cell attachment. Functionalization of the microstructures is also presented that enables cell attachment to them within a few seconds with strength much higher that the optical forces. Fast cell actuation in 6 degrees of freedom is demonstrated with the outlook to possible applications in cell imaging. LA - English DB - MTMT ER - TY - JOUR AU - Vizsnyiczai, Gaszton AU - Lestyán, T AU - Joniova, J AU - Aekbote Lakshman Rao, Badri AU - Strejčková, A AU - Ormos, Pál AU - Miskovsky, P AU - Kelemen, Lóránd AU - Bánó, G TI - Optically Trapped Surface-Enhanced Raman Probes Prepared by Silver Photoreduction to 3D Microstructures JF - LANGMUIR J2 - LANGMUIR VL - 31 PY - 2015 IS - 36 SP - 10087 EP - 10093 PG - 7 SN - 0743-7463 DO - 10.1021/acs.langmuir.5b01210 UR - https://m2.mtmt.hu/api/publication/2946588 ID - 2946588 AB - 3D microstructures partially covered by silver nanoparticles have been developed and tested for surface-enhanced Raman spectroscopy (SERS) in combination with optical tweezers. The microstructures made by two-photon polymerization of SU-8 photoresist were manipulated in a dual beam optical trap. The active area of the structures was covered by a SERS-active silver layer using chemically assisted photoreduction from silver nitrate solutions. Silver layers of different grain size distributions were created by changing the photoreduction parameters and characterized by scanning electron microscopy. The structures were tested by measuring the SERS spectra of emodin and hypericin. © 2015 American Chemical Society. LA - English DB - MTMT ER - TY - JOUR AU - Aekbote Lakshman Rao, Badri AU - Jacak, J AU - Schütz, G J AU - Csányi, Erzsébet AU - Szegletes, Zsolt AU - Ormos, Pál AU - Kelemen, Lóránd TI - Aminosilane-based functionalization of two-photon polymerized 3D SU-8 microstructures JF - EUROPEAN POLYMER JOURNAL J2 - EUR POLYM J VL - 48 PY - 2012 IS - 10 SP - 1745 EP - 1754 PG - 10 SN - 0014-3057 DO - 10.1016/j.eurpolymj.2012.06.011 UR - https://m2.mtmt.hu/api/publication/2077010 ID - 2077010 N1 - Biological Research Centre, Hungarian Academy of Sciences, Institute of Biophysics, Temesvári krt. 62, Szeged H-6726, Hungary Biophysics Institute, Johannes Kepler University Linz, Altenbergerstrasse 69, A-4040 Linz, Austria University of Szeged, Faculty of Pharmacy, Department of Pharmaceutical Technology, Eötvös u. 6, Szeged H-6721, Hungary Cited By :20 Export Date: 27 April 2021 CODEN: EUPJA Correspondence Address: Kelemen, L.; Biological Research Centre, Temesvári krt. 62, Szeged H-6726, Hungary; email: kelemen.lorand@brc.mta.hu LA - English DB - MTMT ER - TY - JOUR AU - Palima, D AU - Banas, AR AU - Vizsnyiczai, Gaszton AU - Kelemen, Lóránd AU - Ormos, Pál AU - Gluckstad, J TI - Wave-guided optical waveguides JF - OPTICS EXPRESS J2 - OPT EXPRESS VL - 20 PY - 2012 IS - 3 SP - 2004 EP - 2014 PG - 11 SN - 1094-4087 DO - 10.1364/OE.20.002004 UR - https://m2.mtmt.hu/api/publication/2015357 ID - 2015357 AB - This work primarily aims to fabricate and use two photon polymerization (2PP) microstructures capable of being optically manipulated into any arbitrary orientation. We have integrated optical waveguides into the structures and therefore have freestanding waveguides, which can be positioned anywhere in the sample at any orientation using optical traps. One of the key aspects to the work is the change in direction of the incident plane wave, and the marked increase in the numerical aperture demonstrated. Hence, the optically steered waveguide can tap from a relatively broader beam and then generate a more tightly confined light at its tip. The paper contains both simulation, related to the propagation of light through the waveguide, and experimental demonstrations using our BioPhotonics Workstation. In a broader context, this work shows that optically trapped microfabricated structures can potentially help bridge the diffraction barrier. This structure-mediated paradigm may be carried forward to open new possibilities for exploiting beams from far-field optics down to the subwavelength domain. (C)2012 Optical Society of America LA - English DB - MTMT ER - TY - JOUR AU - Rodrigo, PJ AU - Kelemen, Lóránd AU - Palima, D AU - Alonzo, CA AU - Ormos, Pál AU - Glukstad, J TI - Optical microassembly platform for constructing reconfigurable microenvironments for biomedical studies JF - OPTICS EXPRESS J2 - OPT EXPRESS VL - 17 PY - 2009 IS - 8 SP - 6578 EP - 6583 PG - 6 SN - 1094-4087 DO - 10.1364/OE.17.006578 UR - https://m2.mtmt.hu/api/publication/1920728 ID - 1920728 LA - English DB - MTMT ER - TY - JOUR AU - Rodrigo, PJ AU - Kelemen, Lóránd AU - Alonzo, CA AU - Perch, Nielsen IR AU - Dam, JS AU - Ormos, Pál AU - Gluckstad, J TI - 2D Optical Manipulation And Assembly of Shape Complementary Planar Microstructures JF - OPTICS EXPRESS J2 - OPT EXPRESS VL - 15 PY - 2007 IS - 14 SP - 9009 EP - 9014 PG - 6 SN - 1094-4087 DO - 10.1364/OE.15.009009 UR - https://m2.mtmt.hu/api/publication/1915121 ID - 1915121 AB - Optical trapping and manipulation offer great flexibility as a non-contact microassembly tool. Its application to the assembly of microscale building blocks may open new doors for micromachine technology. In this work, we demonstrate all-optical assembly of microscopic puzzle pieces in a fluidic environment using programmable arrays of trapping beams. Identical shape-complimentary pieces are optically fabricated with submicron resolution using two-photon polymerization (2PP) technique. These are efficiently assembled into space-filling tessellations by a multiple-beam optical micromanipulation system. The flexibility of the system allows us to demonstrate both user-interactive and computer-automated modes of serial and parallel assembly of microscale objects with high spatial and angular positioning precision. (c) 2007 Optical Society of America. LA - English DB - MTMT ER - TY - JOUR AU - Galajda, Péter AU - Ormos, Pál TI - Complex micromachines produced and driven by light JF - APPLIED PHYSICS LETTERS J2 - APPL PHYS LETT VL - 78 PY - 2001 SP - 249 EP - 251 PG - 3 SN - 0003-6951 DO - 10.1063/1.1339258 UR - https://m2.mtmt.hu/api/publication/1912041 ID - 1912041 LA - English DB - MTMT ER -