@article{MTMT:3289900,
	title = {Light controlled 3D micromotors powered by bacteria},
	url = {https://m2.mtmt.hu/api/publication/3289900},
	author = {Vizsnyiczai, Gaszton and Frangipane, G and Maggi, C and Saglimbeni, F and Bianchi, S and Di Leonardo, R},
	doi = {10.1038/ncomms15974},
	journal-iso = {NAT COMMUN},
	journal = {NATURE COMMUNICATIONS},
	volume = {8},
	unique-id = {3289900},
	issn = {2041-1723},
	abstract = {Self-propelled bacteria can be integrated into synthetic micromachines and act as biological propellers. So far, proposed designs suffer from low reproducibility, large noise levels or lack of tunability. Here we demonstrate that fast, reliable and tunable bio-hybrid micromotors can be obtained by the self-assembly of synthetic structures with genetically engineered biological propellers. The synthetic components consist of 3D interconnected structures having a rotating unit that can capture individual bacteria into an array of microchambers so that cells contribute maximally to the applied torque. Bacterial cells are smooth swimmers expressing a light-driven proton pump that allows to optically control their swimming speed. Using a spatial light modulator, we can address individual motors with tunable light intensities allowing the dynamic control of their rotational speeds. Applying a real-time feedback control loop, we can also command a set of micromotors to rotate in unison with a prescribed angular speed.},
	year = {2017},
	eissn = {2041-1723},
	orcid-numbers = {Vizsnyiczai, Gaszton/0000-0003-3245-3736}
}

@article{MTMT:3027725,
	title = {Surface-modified complex SU-8 microstructures for indirect optical manipulation of single cells},
	url = {https://m2.mtmt.hu/api/publication/3027725},
	author = {Aekbote Lakshman Rao, Badri and Fekete, Tamás and Jacak, J and Vizsnyiczai, Gaszton and Ormos, Pál and Kelemen, Lóránd},
	doi = {10.1364/BOE.7.000045},
	journal-iso = {BIOMED OPT EXPRESS},
	journal = {BIOMEDICAL OPTICS EXPRESS},
	volume = {7},
	unique-id = {3027725},
	issn = {2156-7085},
	abstract = {We introduce a method that combines two-photon polymerization (TPP) and surface functionalization to enable the indirect optical manipulation of live cells. TPP-made 3D microstructures were coated specifically with a multilayer of the protein streptavidin and non-specifically with IgG antibody using polyethylene glycol diamine as a linker molecule. Protein density on their surfaces was quantified for various coating methods. The streptavidin-coated structures were shown to attach to biotinated cells reproducibly. We performed basic indirect optical micromanipulation tasks with attached structure-cell couples using complex structures and a multi-focus optical trap. The use of such extended manipulators for indirect optical trapping ensures to keep a safe distance between the trapping beams and the sensitive cell and enables their 6 degrees of freedom actuation. (C)2015 Optical Society of America},
	keywords = {DAMAGE; ESCHERICHIA-COLI; DEPENDENCE; WAVELENGTH; ADSORPTION; FABRICATION; MICROFLUIDIC SYSTEMS; force spectroscopy; TWEEZERS; 2-PHOTON POLYMERIZATION},
	year = {2016},
	pages = {45-56},
	orcid-numbers = {Fekete, Tamás/0000-0002-1557-845X; Vizsnyiczai, Gaszton/0000-0003-3245-3736; Ormos, Pál/0000-0003-2541-5686; Kelemen, Lóránd/0000-0001-7772-2165}
}

@article{MTMT:3120643,
	title = {High accuracy indirect optical manipulation of live cells with functionalized microtools},
	url = {https://m2.mtmt.hu/api/publication/3120643},
	author = {Vizsnyiczai, Gaszton and Aekbote Lakshman Rao, Badri and Búzás, András and Grexa, István and Ormos, Pál and Kelemen, Lóránd},
	doi = {10.1117/12.2237601},
	journal-iso = {PROCEEDINGS OF SPIE},
	journal = {PROCEEDINGS OF SPIE - THE INTERNATIONAL SOCIETY FOR OPTICAL ENGINEERING},
	volume = {9922},
	unique-id = {3120643},
	issn = {0277-786X},
	abstract = {Optical micro manipulation of live cells has been extensively used to study a wide range of cellular phenomena with relevance in basic research or in diagnostics. The approaches span from manipulation of many cells for high throughput measurement or sorting, to more elaborated studies of intracellular events on trapped single cells when coupled with modern imaging techniques. In case of direct cell trapping the damaging effects of light-cell interaction must be minimized, for instance with the choice of proper laser wavelength. Microbeads have already been used for trapping cells indirectly thereby reducing the irradiation damage and increasing trapping efficiency with their high refractive index contrast. We show here that such intermediate objects can be tailor-made for indirect cell trapping to further increase cell-to-focal spot distance while maintaining their free and fast maneuverability. Carefully designed structures were produced with two-photon polymerization with shapes optimized for effective manipulation and cell attachment. Functionalization of the microstructures is also presented that enables cell attachment to them within a few seconds with strength much higher that the optical forces. Fast cell actuation in 6 degrees of freedom is demonstrated with the outlook to possible applications in cell imaging.},
	year = {2016},
	eissn = {1996-756X},
	orcid-numbers = {Vizsnyiczai, Gaszton/0000-0003-3245-3736; Ormos, Pál/0000-0003-2541-5686; Kelemen, Lóránd/0000-0001-7772-2165}
}

@article{MTMT:2946588,
	title = {Optically Trapped Surface-Enhanced Raman Probes Prepared by Silver Photoreduction to 3D Microstructures},
	url = {https://m2.mtmt.hu/api/publication/2946588},
	author = {Vizsnyiczai, Gaszton and Lestyán, T and Joniova, J and Aekbote Lakshman Rao, Badri and Strejčková, A and Ormos, Pál and Miskovsky, P and Kelemen, Lóránd and Bánó, G},
	doi = {10.1021/acs.langmuir.5b01210},
	journal-iso = {LANGMUIR},
	journal = {LANGMUIR},
	volume = {31},
	unique-id = {2946588},
	issn = {0743-7463},
	abstract = {3D microstructures partially covered by silver nanoparticles have been developed and tested for surface-enhanced Raman spectroscopy (SERS) in combination with optical tweezers. The microstructures made by two-photon polymerization of SU-8 photoresist were manipulated in a dual beam optical trap. The active area of the structures was covered by a SERS-active silver layer using chemically assisted photoreduction from silver nitrate solutions. Silver layers of different grain size distributions were created by changing the photoreduction parameters and characterized by scanning electron microscopy. The structures were tested by measuring the SERS spectra of emodin and hypericin. © 2015 American Chemical Society.},
	keywords = {microstructure; SILVER; scanning electron microscopy; raman spectroscopy; Grain size and shape; Surface enhanced Raman spectroscopy; Photoresists; Optical Tweezers; Grain Size Distribution; Silver nanoparticles; Metallographic microstructure; 3D microstructures; Two photon polymerization; SU-8 photoresist; Trapped surfaces; Silver nitrate solutions},
	year = {2015},
	eissn = {1520-5827},
	pages = {10087-10093},
	orcid-numbers = {Vizsnyiczai, Gaszton/0000-0003-3245-3736; Ormos, Pál/0000-0003-2541-5686; Kelemen, Lóránd/0000-0001-7772-2165}
}

@article{MTMT:2077010,
	title = {Aminosilane-based functionalization of two-photon polymerized 3D SU-8 microstructures},
	url = {https://m2.mtmt.hu/api/publication/2077010},
	author = {Aekbote Lakshman Rao, Badri and Jacak, J and Schütz, G J and Csányi, Erzsébet and Szegletes, Zsolt and Ormos, Pál and Kelemen, Lóránd},
	doi = {10.1016/j.eurpolymj.2012.06.011},
	journal-iso = {EUR POLYM J},
	journal = {EUROPEAN POLYMER JOURNAL},
	volume = {48},
	unique-id = {2077010},
	issn = {0014-3057},
	year = {2012},
	eissn = {1873-1945},
	pages = {1745-1754},
	orcid-numbers = {Csányi, Erzsébet/0000-0002-3010-1959; Szegletes, Zsolt/0000-0003-2202-6933; Ormos, Pál/0000-0003-2541-5686; Kelemen, Lóránd/0000-0001-7772-2165}
}

@article{MTMT:2015357,
	title = {Wave-guided optical waveguides},
	url = {https://m2.mtmt.hu/api/publication/2015357},
	author = {Palima, D and Banas, AR and Vizsnyiczai, Gaszton and Kelemen, Lóránd and Ormos, Pál and Gluckstad, J},
	doi = {10.1364/OE.20.002004},
	journal-iso = {OPT EXPRESS},
	journal = {OPTICS EXPRESS},
	volume = {20},
	unique-id = {2015357},
	issn = {1094-4087},
	abstract = {This work primarily aims to fabricate and use two photon polymerization (2PP) microstructures capable of being optically manipulated into any arbitrary orientation. We have integrated optical waveguides into the structures and therefore have freestanding waveguides, which can be positioned anywhere in the sample at any orientation using optical traps. One of the key aspects to the work is the change in direction of the incident plane wave, and the marked increase in the numerical aperture demonstrated. Hence, the optically steered waveguide can tap from a relatively broader beam and then generate a more tightly confined light at its tip. The paper contains both simulation, related to the propagation of light through the waveguide, and experimental demonstrations using our BioPhotonics Workstation. In a broader context, this work shows that optically trapped microfabricated structures can potentially help bridge the diffraction barrier. This structure-mediated paradigm may be carried forward to open new possibilities for exploiting beams from far-field optics down to the subwavelength domain. (C)2012 Optical Society of America},
	year = {2012},
	pages = {2004-2014},
	orcid-numbers = {Vizsnyiczai, Gaszton/0000-0003-3245-3736; Kelemen, Lóránd/0000-0001-7772-2165; Ormos, Pál/0000-0003-2541-5686}
}

@article{MTMT:1920728,
	title = {Optical microassembly platform for constructing reconfigurable microenvironments for biomedical studies},
	url = {https://m2.mtmt.hu/api/publication/1920728},
	author = {Rodrigo, PJ and Kelemen, Lóránd and Palima, D and Alonzo, CA and Ormos, Pál and Glukstad, J},
	doi = {10.1364/OE.17.006578},
	journal-iso = {OPT EXPRESS},
	journal = {OPTICS EXPRESS},
	volume = {17},
	unique-id = {1920728},
	issn = {1094-4087},
	year = {2009},
	pages = {6578-6583},
	orcid-numbers = {Kelemen, Lóránd/0000-0001-7772-2165; Ormos, Pál/0000-0003-2541-5686}
}

@article{MTMT:1915121,
	title = {2D Optical Manipulation And Assembly of Shape Complementary Planar Microstructures},
	url = {https://m2.mtmt.hu/api/publication/1915121},
	author = {Rodrigo, PJ and Kelemen, Lóránd and Alonzo, CA and Perch, Nielsen IR and Dam, JS and Ormos, Pál and Gluckstad, J},
	doi = {10.1364/OE.15.009009},
	journal-iso = {OPT EXPRESS},
	journal = {OPTICS EXPRESS},
	volume = {15},
	unique-id = {1915121},
	issn = {1094-4087},
	abstract = {Optical trapping and manipulation offer great flexibility as a non-contact microassembly tool. Its application to the assembly of microscale building blocks may open new doors for micromachine technology. In this work, we demonstrate all-optical assembly of microscopic puzzle pieces in a fluidic environment using programmable arrays of trapping beams. Identical shape-complimentary pieces are optically fabricated with submicron resolution using two-photon polymerization (2PP) technique. These are efficiently assembled into space-filling tessellations by a multiple-beam optical micromanipulation system. The flexibility of the system allows us to demonstrate both user-interactive and computer-automated modes of serial and parallel assembly of microscale objects with high spatial and angular positioning precision. (c) 2007 Optical Society of America.},
	year = {2007},
	pages = {9009-9014},
	orcid-numbers = {Kelemen, Lóránd/0000-0001-7772-2165; Ormos, Pál/0000-0003-2541-5686}
}

@article{MTMT:1912041,
	title = {Complex micromachines produced and driven by light},
	url = {https://m2.mtmt.hu/api/publication/1912041},
	author = {Galajda, Péter and Ormos, Pál},
	doi = {10.1063/1.1339258},
	journal-iso = {APPL PHYS LETT},
	journal = {APPLIED PHYSICS LETTERS},
	volume = {78},
	unique-id = {1912041},
	issn = {0003-6951},
	year = {2001},
	eissn = {1077-3118},
	pages = {249-251},
	orcid-numbers = {Ormos, Pál/0000-0003-2541-5686}
}