@article{MTMT:3096913, title = {Transdifferentiation and Proliferation in Two Distinct Hemocyte Lineages in Drosophila melanogaster Larvae after Wasp Infection.}, url = {https://m2.mtmt.hu/api/publication/3096913}, author = {Anderl, I and Vesala, L and Ihalainen, TO and Vanha-Aho, LM and Andó, István and Ramet, M and Hultmark, D}, doi = {10.1371/journal.ppat.1005746}, journal-iso = {PLOS PATHOG}, journal = {PLOS PATHOGENS}, volume = {12}, unique-id = {3096913}, issn = {1553-7366}, abstract = {Cellular immune responses require the generation and recruitment of diverse blood cell types that recognize and kill pathogens. In Drosophila melanogaster larvae, immune-inducible lamellocytes participate in recognizing and killing parasitoid wasp eggs. However, the sequence of events required for lamellocyte generation remains controversial. To study the cellular immune system, we developed a flow cytometry approach using in vivo reporters for lamellocytes as well as for plasmatocytes, the main hemocyte type in healthy larvae. We found that two different blood cell lineages, the plasmatocyte and lamellocyte lineages, contribute to the generation of lamellocytes in a demand-adapted hematopoietic process. Plasmatocytes transdifferentiate into lamellocyte-like cells in situ directly on the wasp egg. In parallel, a novel population of infection-induced cells, which we named lamelloblasts, appears in the circulation. Lamelloblasts proliferate vigorously and develop into the major class of circulating lamellocytes. Our data indicate that lamellocyte differentiation upon wasp parasitism is a plastic and dynamic process. Flow cytometry with in vivo hemocyte reporters can be used to study this phenomenon in detail.}, year = {2016}, eissn = {1553-7374}, pages = {e1005746}, orcid-numbers = {Andó, István/0000-0002-4648-9396} } @article{MTMT:2372553, title = {The cell-mediated immunity of Drosophila melanogaster: Hemocyte lineages, immune compartments, microanatomy and regulation.}, url = {https://m2.mtmt.hu/api/publication/2372553}, author = {Honti, Viktor and Csordás, Gábor and Kurucz, Judit Éva and Márkus, Róbert and Andó, István}, doi = {10.1016/j.dci.2013.06.005}, journal-iso = {DEV COMP IMMUNOL}, journal = {DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY}, volume = {42}, unique-id = {2372553}, issn = {0145-305X}, abstract = {In the animal kingdom, innate immunity is the first line of defense against invading pathogens. The dangers of microbial and parasitic attacks are countered by similar mechanisms, involving the prototypes of the cell-mediated immune responses, the phagocytosis and encapsulation. Work on Drosophila has played an important role in promoting an understanding of the basic mechanisms of phylogenetically conserved modules of innate immunity. The aim of this review is to survey the developments in the identification and functional definition of immune cell types and the immunological compartments of Drosophila melanogaster. We focus on the molecular and developmental aspects of the blood cell types and compartments, as well as the dynamics of blood cell development and the immune response. Further advances in the characterization of the innate immune mechanisms in Drosophila will provide basic clues to the understanding of the importance of the evolutionary conserved mechanisms of innate immune defenses in the animal kingdom.}, year = {2014}, eissn = {1879-0089}, pages = {47-56}, orcid-numbers = {Csordás, Gábor/0000-0001-6871-6839; Andó, István/0000-0002-4648-9396} } @article{MTMT:1920757, title = {Sessile hemocytes as a hematopoietic compartment in drosophila melanogaster}, url = {https://m2.mtmt.hu/api/publication/1920757}, author = {Márkus, Róbert and Laurinyecz, Barbara and Kurucz, Judit Éva and Honti, Viktor and Bajusz, Izabella and Sipos, Botond and Somogyi, Kálmán and Kronhamn, J and Hultmark, D and Andó, István}, doi = {10.1073/pnas.0801766106}, journal-iso = {P NATL ACAD SCI USA}, journal = {PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA}, volume = {106}, unique-id = {1920757}, issn = {0027-8424}, year = {2009}, eissn = {1091-6490}, pages = {4805-4809}, orcid-numbers = {Laurinyecz, Barbara/0000-0003-0620-2239; Andó, István/0000-0002-4648-9396} } @article{MTMT:1915261, title = {Definition of Drosophila hemocyte subsets by cell-type specific antigens}, url = {https://m2.mtmt.hu/api/publication/1915261}, author = {Kurucz, Judit Éva and Váczi, Balázs and Márkus, Róbert and Laurinyecz, Barbara and Vilmos, Péter and Zsámboki, János and Csorba, Kinga and Gateff, E and Hultmark, D and Andó, István}, doi = {10.1556/ABiol.58.2007.Suppl.8}, journal-iso = {ACTA BIOL HUNG}, journal = {ACTA BIOLOGICA HUNGARICA (1983-2018)}, volume = {58}, unique-id = {1915261}, issn = {0236-5383}, abstract = {We analyzed the heterogeneity of Drosophila hemocytes on the basis of the expression of cell-type specific antigens. The antigens characterize distinct subsets which partially overlap with those defined by morphological criteria. Oil the basis of the expression or the lack of expression of blood cell antigens the following hemocyte populations have been defined: crystal cells, plasmalocytes, lamellocytes and precursor cells. The expression of the antigens and thus the different cell types are developmentally regulated. The hemocytes are arranged ill four main compartments: the circulating blood cells, the sessile tissue, the lymph glands and the posterior hematopoietic tissue. Each hemocyte compartment has a specific and characteristic composition of the various cell types. The described markers represent the first successful attempt to define hemocyte lineages by immunological markers in Drosophila and help to define morphologically, functionally, spatially and developmentally distinct subsets of hemocyles.}, year = {2007}, eissn = {1588-256X}, pages = {95-111}, orcid-numbers = {Laurinyecz, Barbara/0000-0003-0620-2239; Andó, István/0000-0002-4648-9396} }