TY - JOUR AU - Csordás, Gábor AU - Varga, Gergely István AU - Honti, Viktor AU - Jankovics, Ferenc AU - Kurucz, Judit Éva AU - Andó, István TI - In Vivo Immunostaining of Hemocyte Compartments in Drosophila for Live Imaging JF - PLOS ONE J2 - PLOS ONE VL - 9 PY - 2014 IS - 6 PG - 6 SN - 1932-6203 DO - 10.1371/journal.pone.0098191 UR - https://m2.mtmt.hu/api/publication/2708773 ID - 2708773 N1 - Institute of Genetics, Biological Research Centre, Hungarian Academy of Sciences, Szeged, Hungary Developmental Genetics Unit, Institute of Genetics, Hungarian Academy of Sciences, Szeged, Hungary Cited By :5 Export Date: 31 January 2020 CODEN: POLNC Institute of Genetics, Biological Research Centre, Hungarian Academy of Sciences, Szeged, Hungary Developmental Genetics Unit, Institute of Genetics, Hungarian Academy of Sciences, Szeged, Hungary Cited By :8 Export Date: 20 April 2021 CODEN: POLNC AB - In recent years, Drosophila melanogaster has become an attractive model organism in which to study the structure and development of the cellular immune components. The emergence of immunological markers greatly accelerated the identification of the immune cells (hemocytes), while the creation of genetic reporter constructs allowed unique insight into the structural organization of hematopoietic tissues. However, investigation of the hemocyte compartments by the means of immunological markers requires dissection and fixation, which regularly disrupt the delicate structure and hamper the microanatomical characterization. Moreover, the investigation of transgenic reporters alone can be misleading as their expression often differs from the native expression pattern of their respective genes. We describe here a method that combines the reporter constructs and the immunological tools in live imaging, thereby allowing use of the array of available immunological markers while retaining the structural integrity of the hematopoietic compartments. The procedure allows the reversible immobilization of Drosophila larvae for high-resolution confocal imaging and the time-lapse video analysis of in vivo reporters. When combined with our antibody injection-based in situ immunostaining assay, the resulting double labeling of the hemocyte compartments can provide new information on the microanatomy and functional properties of the hematopoietic tissues in an intact state. Although this method was developed to study the immune system of Drosophila melanogaster, we anticipate that such a combination of genetic and immunological markers could become a versatile technique for in vivo studies in other biological systems too. LA - English DB - MTMT ER - TY - JOUR AU - Gillingham, Alison K AU - Sinka, Rita AU - Torres, Isabel L AU - Lilley, Kathryn S AU - Munro, Sean TI - Toward a Comprehensive Map of the Effectors of Rab GTPases JF - DEVELOPMENTAL CELL J2 - DEV CELL VL - 31 PY - 2014 IS - 3 SP - 358 EP - 373 PG - 16 SN - 1534-5807 DO - 10.1016/j.devcel.2014.10.007 UR - https://m2.mtmt.hu/api/publication/2827923 ID - 2827923 LA - English DB - MTMT ER - TY - JOUR AU - Takáts, Szabolcs AU - Pircs, Karolina Milena AU - Nagy, Péter AU - Varga, Ágnes AU - Kárpáti, Manuéla AU - Hegedűs, Krisztina AU - Kramer, H AU - Kovács, Attila Lajos AU - Sass, Miklós AU - Juhász, Gábor TI - Interaction of the HOPS complex with Syntaxin 17 mediates autophagosome clearance in Drosophila JF - MOLECULAR BIOLOGY OF THE CELL J2 - MOL BIOL CELL VL - 25 PY - 2014 IS - 8 SP - 1338 EP - 1354 PG - 17 SN - 1059-1524 DO - 10.1091/mbc.E13-08-0449 UR - https://m2.mtmt.hu/api/publication/2527687 ID - 2527687 N1 - Department of Anatomy, Cell and Developmental Biology, Eötvös Loránd University, Pazmany s. 1/C, H-1117 Budapest, Hungary Department of Neuroscience, Department of Cell Biology, University of Texas Southwestern Medical School, Dallas, TX, United States Cited By :138 Export Date: 4 May 2021 CODEN: MBCEE Correspondence Address: Juhász, G.; Department of Anatomy, Cell and Developmental Biology, Eötvös Loránd University, Pazmany s. 1/C, H-1117 Budapest, Hungary; email: szmrt@elte.hu LA - English DB - MTMT ER - TY - JOUR AU - Takáts, Szabolcs AU - Nagy, Péter AU - Varga, Ágnes AU - Pircs, Karolina Milena AU - Kárpáti, Manuéla AU - Varga, Kata AU - Kovács, Attila Lajos AU - Hegedűs, Krisztina AU - Juhász, Gábor TI - Autophagosomal Syntaxin17-dependent lysosomal degradation maintains neuronal function in Drosophila. JF - JOURNAL OF CELL BIOLOGY J2 - J CELL BIOL VL - 201 PY - 2013 IS - 4 SP - 531 EP - 539 PG - 9 SN - 0021-9525 DO - 10.1083/jcb.201211160 UR - https://m2.mtmt.hu/api/publication/2328740 ID - 2328740 N1 - Cited By :208 Export Date: 27 June 2022 CODEN: JCLBA Correspondence Address: Takáts, S.; Department of Anatomy, Cell and Developmental Biology, , H-1117 Budapest, Hungary; email: szmrt@elte.hu AB - During autophagy, phagophores capture portions of cytoplasm and form double-membrane autophagosomes to deliver cargo for lysosomal degradation. How autophagosomes gain competence to fuse with late endosomes and lysosomes is not known. In this paper, we show that Syntaxin17 is recruited to the outer membrane of autophagosomes to mediate fusion through its interactions with ubisnap (SNAP-29) and VAMP7 in Drosophila melanogaster. Loss of these genes results in accumulation of autophagosomes and a block of autolysosomal degradation during basal, starvation-induced, and developmental autophagy. Viable Syntaxin17 mutant adults show large-scale accumulation of autophagosomes in neurons, severe locomotion defects, and premature death. These mutant phenotypes cannot be rescued by neuron-specific inhibition of caspases, suggesting that caspase activation and cell death do not play a major role in brain dysfunction. Our findings reveal the molecular mechanism underlying autophagosomal fusion events and show that lysosomal degradation and recycling of sequestered autophagosome content is crucial to maintain proper functioning of the nervous system. LA - English DB - MTMT ER - TY - JOUR AU - Márkus, Róbert AU - Laurinyecz, Barbara AU - Kurucz, Judit Éva AU - Honti, Viktor AU - Bajusz, Izabella AU - Sipos, Botond AU - Somogyi, Kálmán AU - Kronhamn, J AU - Hultmark, D AU - Andó, István TI - Sessile hemocytes as a hematopoietic compartment in drosophila melanogaster JF - PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA J2 - P NATL ACAD SCI USA VL - 106 PY - 2009 IS - 12 SP - 4805 EP - 4809 PG - 5 SN - 0027-8424 DO - 10.1073/pnas.0801766106 UR - https://m2.mtmt.hu/api/publication/1920757 ID - 1920757 N1 - Cited By :174 Export Date: 30 June 2022 CODEN: PNASA LA - English DB - MTMT ER - TY - JOUR AU - Kurucz, Judit Éva AU - Váczi, Balázs AU - Márkus, Róbert AU - Laurinyecz, Barbara AU - Vilmos, Péter AU - Zsámboki, János AU - Csorba, Kinga AU - Gateff, E AU - Hultmark, D AU - Andó, István TI - Definition of Drosophila hemocyte subsets by cell-type specific antigens JF - ACTA BIOLOGICA HUNGARICA (1983-2018) J2 - ACTA BIOL HUNG VL - 58 PY - 2007 IS - Suppl. 1 SP - 95 EP - 111 PG - 17 SN - 0236-5383 DO - 10.1556/ABiol.58.2007.Suppl.8 UR - https://m2.mtmt.hu/api/publication/1915261 ID - 1915261 AB - We analyzed the heterogeneity of Drosophila hemocytes on the basis of the expression of cell-type specific antigens. The antigens characterize distinct subsets which partially overlap with those defined by morphological criteria. Oil the basis of the expression or the lack of expression of blood cell antigens the following hemocyte populations have been defined: crystal cells, plasmalocytes, lamellocytes and precursor cells. The expression of the antigens and thus the different cell types are developmentally regulated. The hemocytes are arranged ill four main compartments: the circulating blood cells, the sessile tissue, the lymph glands and the posterior hematopoietic tissue. Each hemocyte compartment has a specific and characteristic composition of the various cell types. The described markers represent the first successful attempt to define hemocyte lineages by immunological markers in Drosophila and help to define morphologically, functionally, spatially and developmentally distinct subsets of hemocyles. LA - English DB - MTMT ER - TY - JOUR AU - Kurucz, Judit Éva AU - Márkus, Róbert AU - Zsámboki, János AU - Medzihradszky F., Katalin AU - Darula, Zsuzsanna AU - Vilmos, Péter AU - Udvardy, Andor AU - Krausz, Ildikó AU - Lukacsovich, Tamás AU - Gateff, E AU - Zettervall, CJ AU - Hultmark, D AU - Andó, István TI - Nimrod, a Putative Phagocytosis Receptor With Egf Repeats in Drosophila Plasmatocytes JF - CURRENT BIOLOGY J2 - CURR BIOL VL - 17 PY - 2007 IS - 7 SP - 649 EP - 654 PG - 6 SN - 0960-9822 DO - 10.1016/j.cub.2007.02.041 UR - https://m2.mtmt.hu/api/publication/1915010 ID - 1915010 N1 - Megjegyzés-22232309 Z9: 60 DI: 10.1016/j.cub.2007.02.041 LA - English DB - MTMT ER -