TY - JOUR AU - Tóth, István AU - Kiss, Dávid Sándor AU - Jócsák, Gergely AU - Somogyi, Virág AU - Toronyi, Éva AU - Bartha, Tibor AU - Frenyó V., László AU - Horváth, Tamás AU - Zsarnovszky, Attila TI - Estrogen- and Satiety State-Dependent Metabolic Lateralization in the Hypothalamus of Female Rats. JF - PLOS ONE J2 - PLOS ONE VL - 10 PY - 2015 IS - 9 PG - 11 SN - 1932-6203 DO - 10.1371/journal.pone.0137462 UR - https://m2.mtmt.hu/api/publication/2940248 ID - 2940248 AB - Hypothalamus is the highest center and the main crossroad of numerous homeostatic regulatory pathways including reproduction and energy metabolism. Previous reports indicate that some of these functions may be driven by the synchronized but distinct functioning of the left and right hypothalamic sides. However, the nature of interplay between the hemispheres with regard to distinct hypothalamic functions is still unclear. Here we investigated the metabolic asymmetry between the left and right hypothalamic sides of ovariectomized female rats by measuring mitochondrial respiration rates, a parameter that reflects the intensity of cell and tissue metabolism. Ovariectomized (saline injected) and ovariectomized+estrogen injected animals were fed ad libitum or fasted to determine 1) the contribution of estrogen to metabolic asymmetry of hypothalamus; and 2) whether the hypothalamic asymmetry is modulated by the satiety state. Results show that estrogen-priming significantly increased both the proportion of animals with detected hypothalamic lateralization and the degree of metabolic difference between the hypothalamic sides causing a right-sided dominance during state 3 mitochondrial respiration (St3) in ad libitum fed animals. After 24 hours of fasting, lateralization in St3 values was clearly maintained; however, instead of the observed right-sided dominance that was detected in ad libitum fed animals here appeared in form of either right- or left-sidedness. In conclusion, our results revealed estrogen- and satiety state-dependent metabolic differences between the two hypothalamic hemispheres in female rats showing that the hypothalamic hemispheres drive the reproductive and satiety state related functions in an asymmetric manner. LA - English DB - MTMT ER - TY - JOUR AU - Andrews, ZB AU - Erion, D AU - Beiler, R AU - Liu, ZW AU - Abizaid, A AU - Zigman, J AU - Elsworth, JD AU - Savitt, JM AU - DiMarchi, R AU - Tschoep, M AU - Roth, RH AU - Gao, XB AU - Horváth, Tamás TI - Ghrelin Promotes and Protects Nigrostriatal Dopamine Function via a UCP2-Dependent Mitochondrial Mechanism JF - JOURNAL OF NEUROSCIENCE J2 - J NEUROSCI VL - 29 PY - 2009 IS - 45 SP - 14057 EP - 14065 PG - 9 SN - 0270-6474 DO - 10.1523/JNEUROSCI.3890-09.2009 UR - https://m2.mtmt.hu/api/publication/3201614 ID - 3201614 LA - English DB - MTMT ER - TY - JOUR AU - Kiss, Dávid Sándor AU - Zsarnovszky, Attila AU - Horvath, Krisztina AU - Győrffy, Andrea AU - Bartha, Tibor AU - Novák-Hazai, Diana AU - Sótonyi, Péter AU - Somogyi, Virág AU - Frenyó V., László AU - Diano, Sabrina TI - Ecto-nucleoside triphosphate diphosphohydrolase 3 in the ventral and lateral hypothalamic area of female rats: morphological characterization and functional implications JF - REPRODUCTIVE BIOLOGY AND ENDOCRINOLOGY J2 - REPROD BIOL ENDOCRIN VL - 7 PY - 2009 PG - 12 SN - 1477-7827 DO - 10.1186/1477-7827-7-31 UR - https://m2.mtmt.hu/api/publication/1228647 ID - 1228647 AB - Abstract Background: Based on its distribution in the brain, ecto-nucleoside triphosphate diphosphohydrolase 3 (NTPDase3) may play a role in the hypothalamic regulation of homeostatic systems, including feeding, sleep-wake behavior and reproduction. To further characterize the morphological attributes of NTPDase3-immunoreactive (IR) hypothalamic structures in the rat brain, here we investigated: 1.) The cellular and subcellular localization of NTPDase3; 2.) The effects of 17-estradiol on the expression level of hypothalamic NTPDase3; and 3.) The effects of NTPDase inhibition in hypothalamic synaptosomal preparations. Methods: Combined light- and electron microscopic analyses were carried out to characterize the cellular and subcellular localization of NTPDase3-immunoreactivity. The effects of estrogen on hypothalamic NTPDase3 expression was studied by western blot technique. Finally, the effects of NTPDase inhibition on mitochondrial respiration were investigated using a Clark-type oxygen electrode. Results: Combined light- and electron microscopic analysis of immunostained hypothalamic slices revealed that NTPDase3-IR is linked to ribosomes and mitochondria, is predominantly present in excitatory axon terminals and in distinct segments of the perikaryal plasma membrane. Immunohistochemical labeling of NTPDase3 and glutamic acid decarboxylase (GAD) indicated that -amino-butyric-acid- (GABA) ergic hypothalamic neurons do not express NTPDase3, further suggesting that in the hypothalamus, NTPDase3 is predominantly present in excitatory neurons. We also investigated whether estrogen influences the expression level of NTPDase3 in the ventrobasal and lateral hypothalamus. A single subcutaneous injection of estrogen differentially increased NTPDase3 expression in the medial and lateral parts of the hypothalamus, indicating that this enzyme likely plays region-specific roles in estrogen-dependent hypothalamic regulatory mechanisms. Determination of mitochondrial respiration rates with and without the inhibition of NTPDases confirmed the presence of NTPDases, including NTPDase3 in neuronal mitochondria and showed that blockade of mitochondrial NTPDase functions decreases state 3 mitochondrial respiration rate and total mitochondrial respiratory capacity. Conclusions: Altogether, these results suggest the possibility that NTPDases, among them NTPDase3, may play an estrogen-dependent modulatory role in the regulation of intracellular availability of ATP needed for excitatory neuronal functions including neurotransmission. LA - English DB - MTMT ER - TY - JOUR AU - Andrews, ZB AU - Liu, ZW AU - Walllingford, N AU - Erion, DM AU - Borok, E AU - Friedman, JM AU - Tschop, MH AU - Shanabrough, M AU - Cline, G AU - Shulman, GI AU - Coppola, A AU - Gao, XB AU - Horváth, Tamás AU - Diano, S TI - UCP2 mediates ghrelin's action on NPY/AgRP neurons by lowering free radicals JF - NATURE J2 - NATURE VL - 454 PY - 2008 IS - 7206 SP - 846 EP - 851 PG - 6 SN - 0028-0836 DO - 10.1038/nature07181 UR - https://m2.mtmt.hu/api/publication/3201232 ID - 3201232 LA - English DB - MTMT ER -