@article{MTMT:3312483,
	title = {Comparison of 2D and 3D neural induction methods for the generation of neural progenitor cells from human induced pluripotent stem cells},
	url = {https://m2.mtmt.hu/api/publication/3312483},
	author = {Chandrasekaran, A and Avci, HX and Ochalek, A and Rosingh, LN and Molnár, Kinga and László, Lajos and Bellák, Tamás and Teglasi, A and Pesti, Krisztina and Mike, Árpád and Phanthong, P and Biró, Orsolya and Hall, V and Kitiyanant, N and Krause, KH and Kobolák, Julianna and Dinnyés, András},
	doi = {10.1016/j.scr.2017.10.010},
	journal-iso = {STEM CELL RES},
	journal = {STEM CELL RESEARCH},
	volume = {25},
	unique-id = {3312483},
	issn = {1873-5061},
	abstract = {Neural progenitor cells (NPCs) from human induced pluripotent stem cells (hiPSCs) are frequently induced using 3D culture methodologies however, it is unknown whether spheroid-based (3D) neural induction is actually superior to monolayer (2D) neural induction. Our aim was to compare the efficiency of 2D induction with 3D induction method in their ability to generate NPCs, and subsequently neurons and astrocytes. Neural differentiation was analysed at the protein level qualitatively by immunocytochemistry and quantitatively by flow cytometry for NPC (SOX1, PAX6, NESTIN), neuronal (MAP2, TUBB3), cortical layer (TBR1, CUX1) and glial markers (SOX9, GFAP, AQP4). Electron microscopy demonstrated that both methods resulted in morphologically similar neural rosettes. However, quantification of NPCs derived from 3D neural induction exhibited an increase in the number of PAX6/NESTIN double positive cells and the derived neurons exhibited longer neurites. In contrast, 2D neural induction resulted in more SOX1 positive cells. While 2D monolayer induction resulted in slightly less mature neurons, at an early stage of differentiation, the patch clamp analysis failed to reveal any significant differences between the electrophysiological properties between the two induction methods. In conclusion, 3D neural induction increases the yield of PAX6(+)/NESTIN(+) cells and gives rise to neurons with longer neurites, which might be an advantage for the production of forebrain cortical neurons, highlighting the potential of 3D neural induction, independent of iPSCs' genetic background.},
	year = {2017},
	eissn = {1876-7753},
	pages = {139-151},
	orcid-numbers = {Molnár, Kinga/0000-0002-7196-5331; László, Lajos/0000-0002-2114-9109; Mike, Árpád/0000-0002-9095-8161; Biró, Orsolya/0000-0002-4300-3602; Kobolák, Julianna/0000-0002-0986-9517}
}

@article{MTMT:3208369,
	title = {Integration of electrophysiological recordings with single-cell RNA-seq data identifies neuronal subtypes.},
	url = {https://m2.mtmt.hu/api/publication/3208369},
	author = {Fuzik, J and Zeisel, A and Máté, Zoltán and Calvigioni, D and Yanagawa, Y and Szabó, Gábor and Linnarsson, S and Harkany, T},
	doi = {10.1038/nbt.3443},
	journal-iso = {NAT BIOTECHNOL},
	journal = {NATURE BIOTECHNOLOGY},
	volume = {34},
	unique-id = {3208369},
	issn = {1087-0156},
	abstract = {Traditionally, neuroscientists have defined the identity of neurons by the cells' location, morphology, connectivity and excitability. However, the direct relationship between these parameters and the molecular phenotypes has remained largely unexplored. Here, we present a method for obtaining full transcriptome data from single neocortical pyramidal cells and interneurons after whole-cell patch-clamp recordings in mouse brain slices. In our approach, termed Patch-seq, a patch-clamp stimulus protocol is followed by the aspiration of the entire somatic compartment into the recording pipette, reverse transcription of RNA including addition of unique molecular identifiers, cDNA amplification, Illumina library preparation and sequencing. We show that Patch-seq reveals a close link between electrophysiological characteristics, responses to acute chemical challenges and RNA expression of neurotransmitter receptors and channels. Moreover, it distinguishes neuronal subpopulations that correspond to both well-established and, to our knowledge, hitherto undescribed neuronal subtypes. Our findings demonstrate the ability of Patch-seq to precisely map neuronal subtypes and predict their network contributions in the brain.},
	keywords = {Animals; MICE; Electrophysiology; Gene Expression Profiling; RNA, Messenger/analysis/metabolism; Patch-Clamp Techniques/*methods; Neurons/*cytology/*metabolism; Single-Cell Analysis/*methods; Sequence Analysis, RNA/*methods},
	year = {2016},
	eissn = {1546-1696},
	pages = {175-183}
}

@article{MTMT:3198991,
	title = {Modeling human cortical development in vitro using induced pluripotent stem cells},
	url = {https://m2.mtmt.hu/api/publication/3198991},
	author = {Mariani, J and Simonini, MV and Palejev, D and Tomasini, L and Coppola, G and Szekely, AM and Horváth, Tamás and Vaccarino, FM},
	doi = {10.1073/pnas.1202944109},
	journal-iso = {P NATL ACAD SCI USA},
	journal = {PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA},
	volume = {109},
	unique-id = {3198991},
	issn = {0027-8424},
	year = {2012},
	eissn = {1091-6490},
	pages = {12770-12775}
}

@article{MTMT:3250820,
	title = {Proposal of a model of mammalian neural induction.},
	url = {https://m2.mtmt.hu/api/publication/3250820},
	author = {Levine, AJ and Brivanlou, Ali H.},
	doi = {10.1016/j.ydbio.2007.05.036},
	journal-iso = {DEV BIOL},
	journal = {DEVELOPMENTAL BIOLOGY},
	volume = {308},
	unique-id = {3250820},
	issn = {0012-1606},
	abstract = {How does the vertebrate embryo make a nervous system? This complex question has been at the center of developmental biology for many years. The earliest step in this process - the induction of neural tissue - is intimately linked to patterning of the entire early embryo, and the molecular and embryological of basis these processes are beginning to emerge. Here, we analyze classic and cutting-edge findings on neural induction in the mouse. We find that data from genetics, tissue explants, tissue grafting, and molecular marker expression support a coherent framework for mammalian neural induction. In this model, the gastrula organizer of the mouse embryo inhibits BMP signaling to allow neural tissue to form as a default fate-in the absence of instructive signals. The first neural tissue induced is anterior and subsequent neural tissue is posteriorized to form the midbrain, hindbrain, and spinal cord. The anterior visceral endoderm protects the pre-specified anterior neural fate from similar posteriorization, allowing formation of forebrain. This model is very similar to the default model of neural induction in the frog, thus bridging the evolutionary gap between amphibians and mammals.},
	keywords = {Animals; MICE; signal transduction; *Models, Neurological; Body Patterning/genetics/physiology; Bone Morphogenetic Proteins/metabolism; Organizers, Embryonic; Nervous System/*embryology; Gastrula/cytology/metabolism; Endoderm/cytology/metabolism; Embryonic Induction/*genetics/*physiology},
	year = {2007},
	eissn = {1095-564X},
	pages = {247-256}
}

@article{MTMT:1410336,
	title = {Neural subtype specification of fertilization and nuclear transfer embryonic stem cells and application in parkinsonian mice},
	url = {https://m2.mtmt.hu/api/publication/1410336},
	author = {Barberi, T and Klivényi, Péter and Calingasan, NY and Lee, H and Kawamata, H and Loonam, K and Perrier, AL and Bruses, J and Rubio, ME and Topf, N and Tabar, V and Harrison, NL and Beal, MF and Moore, MA and Studer, L},
	doi = {10.1038/nbt870},
	journal-iso = {NAT BIOTECHNOL},
	journal = {NATURE BIOTECHNOLOGY},
	volume = {21},
	unique-id = {1410336},
	issn = {1087-0156},
	year = {2003},
	eissn = {1546-1696},
	pages = {1200-1207},
	orcid-numbers = {Klivényi, Péter/0000-0002-5389-3266}
}

@article{MTMT:3250855,
	title = {Neural induction, the default model and embryonic stem cells.},
	url = {https://m2.mtmt.hu/api/publication/3250855},
	author = {Munoz-Sanjuan, I and Brivanlou, Ali H.},
	doi = {10.1038/nrn786},
	journal-iso = {NAT REV NEUROSCI},
	journal = {NATURE REVIEWS NEUROSCIENCE},
	volume = {3},
	unique-id = {3250855},
	issn = {1471-003X},
	keywords = {Animals; Humans; Neurons/*physiology; Signal Transduction/*physiology; Models, Neurological; Proto-Oncogene Proteins/metabolism; Insulin-Like Growth Factor I/metabolism; *Zebrafish Proteins; Wnt Proteins; Stem Cells/*physiology; Cell Lineage; Embryo, Nonmammalian/anatomy & histology/physiology; Bone Morphogenetic Proteins/*metabolism; Fibroblast Growth Factors/metabolism; Embryonic Induction/*physiology; Organizers, Embryonic/physiology},
	year = {2002},
	eissn = {1471-0048},
	pages = {271-280}
}