@article{MTMT:3273020,
	title = {Various levels of circulating exosomal total-miRNA and miR-210 hypoxamiR in different forms of pregnancy hypertension},
	url = {https://m2.mtmt.hu/api/publication/3273020},
	author = {Biró, Orsolya and Alasztics, Bálint and Molvarec, Attila and Joó, József Gábor and Nagy, Bálint and Rigó, János},
	doi = {10.1016/j.preghy.2017.09.002},
	journal-iso = {PREGNANCY HYPERTENS},
	journal = {PREGNANCY HYPERTENSION},
	volume = {10},
	unique-id = {3273020},
	issn = {2210-7789},
	abstract = {Introduction: Hypertension is a common complication during pregnancy, affecting 10% of pregnant women worldwide. Several microRNA (miRNA) were shown to be involved in hypertensive disorders of pregnancy. In preeclampsia (PE), placental dysfunction causes the enhanced release of extracellular vesicle-derived miRNAs. The hypoxia-sensitive hsa-mir-210 is the most common PE-associated miRNA, but its exosomal profile has not been investigated. Objectives: Our aims were to measure exosomal total-miRNA concentration and to perform expression analysis of circulating exosomal hsa-miR-210 in women affected by chronic hypertension (CHT) gestational hypertension (GHT) or PE. Materials and methods: We collected plasma samples from women with CHT, GHT, PE (moderate: mPE and severe: sPE) and from normotensive pregnancies. Exosomal miRNAs were extracted and miRNA concentration was measured. RT-PCR was carried out with hsa-miR-210-3p-specific primers and relative expression was calculated using the comparative Ct method. Results: The total-miRNA concentration was different in the disease subgroups, and was significantly higher in mPE and sPE compared to the other groups. We found a significant difference in the relative exosomal hsa-miR-210-3p expression between all hypertensive groups compared to the normotensive samples, but significant upregulation was only observed in case of mPE and sPE patients. Both the level of total-miRNA and hsa-miR-210 expression was higher in case of severe PE. Conclusions: The level of circulating exosomal total-miRNA and hsa-miR-210 was elevated in women with PE, and it was higher in the severe form. We showed that hsa-miR-210 is secreted via exosomes, which may have a role in the pathomechanism of the disease. © 2017 International Society for the Study of Hypertension in Pregnancy.},
	keywords = {PREECLAMPSIA; microRNA; exosome; Maternal circulation},
	year = {2017},
	eissn = {2210-7797},
	pages = {207-212},
	orcid-numbers = {Biró, Orsolya/0000-0002-4300-3602; Alasztics, Bálint/0000-0002-4011-8439; Molvarec, Attila/0000-0002-3229-3034; Joó, József Gábor/0000-0001-9820-6514; Nagy, Bálint/0000-0002-0295-185X; Rigó, János/0000-0003-2762-6516}
}

@article{MTMT:3092009,
	title = {Radiolabeling of Extracellular Vesicles with (99m)Tc for Quantitative In Vivo Imaging Studies.},
	url = {https://m2.mtmt.hu/api/publication/3092009},
	author = {Varga, Zoltán and Gyurko, I and Pálóczi, Krisztina and Buzás, Edit Irén and Horvath, I and Hegedűs, Nikolett and Máthé, Domokos and Szigeti, Krisztián},
	doi = {10.1089/cbr.2016.2009},
	journal-iso = {CANCER BIOTHER RADIO},
	journal = {CANCER BIOTHERAPY AND RADIOPHARMACEUTICALS},
	volume = {31},
	unique-id = {3092009},
	issn = {1084-9785},
	abstract = {The biodistribution of extracellular vesicles (EVs) is a fundamental question in the field of circulating biomarkers, which has recently gained attention. Despite the capabilities of nuclear imaging methods, such as single-photon emission computed tomography, radioisotope labeling of EVs and the use of the aforementioned methods for in vivo studies hardly can be found in the literature. In this article, the authors describe a novel method for the radioisotope labeling of erythrocyte-derived EVs using the (99m)Tc-tricarbonyl complex. Moreover, the capability of the developed labeling method for in vivo biodistribution studies is demonstrated in a mouse model. The authors found that the intravenously administered (99m)Tc-labeled EVs mostly accumulated in the liver and spleen. The in vivo stability of the labeled EVs was assessed by the comparison of the obtained biodistribution of EVs with that of the free (99m)Tc-tricarbonyl. According to the authors' data, only a minor fraction of the radioactive label became detached from the EVs.},
	year = {2016},
	eissn = {1557-8852},
	pages = {168-173},
	orcid-numbers = {Varga, Zoltán/0000-0002-5741-2669; Pálóczi, Krisztina/0000-0001-7065-3582; Buzás, Edit Irén/0000-0002-3744-206X; Hegedűs, Nikolett/0000-0003-1122-1872}
}

@article{MTMT:3106717,
	title = {Exosomes derived from alcohol-treated hepatocytes horizontally transfer liver specific miRNA-122 and sensitize monocytes to LPS},
	url = {https://m2.mtmt.hu/api/publication/3106717},
	author = {Momen-Heravi, F and Bala, S and Kodys, K and Szabó, Gyöngyi},
	doi = {10.1038/srep09991},
	journal-iso = {SCI REP},
	journal = {SCIENTIFIC REPORTS},
	volume = {5},
	unique-id = {3106717},
	issn = {2045-2322},
	abstract = {Hepatocyte damage and inflammation in monocytes/macrophages are central to the pathogenesis of alcoholic hepatitis (AH). MicroRNAs (miRNAs) regulate all of these processes. MiRNA-122 is abundantly expressed in hepatocytes while monocytes/macrophages have low levels. The role of exosomes in AH and possible cross talk between hepatocyte-derived exosomes and immune cells is not explored yet. Here, we show that the number of exosomes significantly increases in the sera of healthy individuals after alcohol binge drinking and in mice after binge or chronic alcohol consumption. Exosomes isolated from sera after alcohol consumption or from in vitro ethanol-treated hepatocytes contained miRNA-122. Exosomes derived from ethanol-treated Huh7.5 cells were taken up by the recipients THP1 monocytes and horizontally transferred a mature form of liver-specific miRNA-122. In vivo, liver mononuclear cells and Kupffer cells from alcohol-fed mice had increased miRNA-122 levels. In monocytes, miRNA-122 transferred via exosomes inhibited the HO-1 pathway and sensitized to LPS stimulation and increased levels of pro-inflammatory cytokines. Finally, inflammatory effects of exosomes from ethanol-treated hepatocytes were prevented by using RNA interference via exosome-mediated delivery of a miRNA-122 inhibitor. These results demonstrate that first, exosomes mediate communication between hepatocytes and monocytes/macrophages and second, hepatocyte-derived miRNA-122 can reprogram monocytes inducing sensitization to LPS.},
	year = {2015},
	eissn = {2045-2322}
}

@article{MTMT:2930099,
	title = {Biological properties of extracellular vesicles and their physiological functions},
	url = {https://m2.mtmt.hu/api/publication/2930099},
	author = {Yanez-Mo, M and Siljander, PR and Andreu, Z and Zavec, AB and Borras, FE and Buzás, Edit Irén and Buzás, Krisztina and Casal, E and Cappello, F and Carvalho, J and Colas, E and Cordeiro-da Silva, A and Fais, S and Falcon-Perez, JM and Ghobrial, IM and Giebel, B and Gimona, M and Graner, M and Gursel, I and Gursel, M and Heegaard, NH and Hendrix, A and Kierulf, P and Kokubun, K and Kosanovic, M and Kralj-Iglic, V and Kramer-Albers, EM and Laitinen, S and Lasser, C and Lener, T and Ligeti, Erzsébet and Line, A and Lipps, G and Llorente, A and Lotvall, J and Mancek-Keber, M and Marcilla, A and Mittelbrunn, M and Nazarenko, I and Nolte-'t, Hoen EN and Nyman, TA and O'Driscoll, L and Olivan, M and Oliveira, C and Pállinger, Éva and Del Portillo, HA and Reventos, J and Rigau, M and Rohde, E and Sammar, M and Sanchez-Madrid, F and Santarem, N and Schallmoser, K and Ostenfeld, MS and Stoorvogel, W and Stukelj, R and Van, der Grein SG and Vasconcelos, MH and Wauben, MH and De Wever, O},
	doi = {10.3402/jev.v4.27066},
	journal-iso = {J EXTRACELLULAR VESICL},
	journal = {JOURNAL OF EXTRACELLULAR VESICLES},
	volume = {4},
	unique-id = {2930099},
	abstract = {In the past decade, extracellular vesicles (EVs) have been recognized as potent vehicles of intercellular communication, both in prokaryotes and eukaryotes. This is due to their capacity to transfer proteins, lipids and nucleic acids, thereby influencing various physiological and pathological functions of both recipient and parent cells. While intensive investigation has targeted the role of EVs in different pathological processes, for example, in cancer and autoimmune diseases, the EV-mediated maintenance of homeostasis and the regulation of physiological functions have remained less explored. Here, we provide a comprehensive overview of the current understanding of the physiological roles of EVs, which has been written by crowd-sourcing, drawing on the unique EV expertise of academia-based scientists, clinicians and industry based in 27 European countries, the United States and Australia. This review is intended to be of relevance to both researchers already working on EV biology and to newcomers who will encounter this universal cell biological system. Therefore, here we address the molecular contents and functions of EVs in various tissues and body fluids from cell systems to organs. We also review the physiological mechanisms of EVs in bacteria, lower eukaryotes and plants to highlight the functional uniformity of this emerging communication system.},
	year = {2015},
	eissn = {2001-3078},
	orcid-numbers = {Buzás, Edit Irén/0000-0002-3744-206X; Buzás, Krisztina/0000-0001-8933-2033; Ligeti, Erzsébet/0000-0001-6374-729X; Pállinger, Éva/0000-0002-5789-0951}
}

@article{MTMT:2516546,
	title = {Emerging role of extracellular vesicles in inflammatory diseases},
	url = {https://m2.mtmt.hu/api/publication/2516546},
	author = {Buzás, Edit Irén and György, Bence and Nagy, György and Falus, András and Gay, Steffen},
	doi = {10.1038/nrrheum.2014.19},
	journal-iso = {NAT REV RHEUMATOL},
	journal = {NATURE REVIEWS RHEUMATOLOGY},
	volume = {10},
	unique-id = {2516546},
	issn = {1759-4790},
	year = {2014},
	eissn = {1759-4804},
	pages = {356-364},
	orcid-numbers = {Buzás, Edit Irén/0000-0002-3744-206X; Nagy, György/0000-0003-1198-3228; Falus, András/0000-0002-6843-6789}
}