TY  - JOUR
AU  - Chandrasekaran, A
AU  - Avci, HX
AU  - Ochalek, A
AU  - Rosingh, LN
AU  - Molnár, Kinga
AU  - László, Lajos
AU  - Bellák, Tamás
AU  - Teglasi, A
AU  - Pesti, Krisztina
AU  - Mike, Árpád
AU  - Phanthong, P
AU  - Biró, Orsolya
AU  - Hall, V
AU  - Kitiyanant, N
AU  - Krause, KH
AU  - Kobolák, Julianna
AU  - Dinnyés, András
TI  - Comparison of 2D and 3D neural induction methods for the generation of neural progenitor cells from human induced pluripotent stem cells
JF  - STEM CELL RESEARCH
J2  - STEM CELL RES
VL  - 25
PY  - 2017
SP  - 139
EP  - 151
PG  - 13
SN  - 1873-5061
DO  - 10.1016/j.scr.2017.10.010
UR  - https://m2.mtmt.hu/api/publication/3312483
ID  - 3312483
N1  - BioTalentum Ltd, Gödöllő, Hungary            
            Department of Anatomy, Embryology and Histology, Faculty of Medicine, University of Szeged, Szeged, Hungary            
            Molecular Animal Biotechnology Lab, Szent István University, Gödöllő, Hungary            
            Department of Pathology and Immunology, University of Geneva Medical School, Geneva, Switzerland            
            Department of Anatomy, Cell and Developmental Biology, Eötvös Loránd University, Budapest, Hungary            
            Opto-Neuropharmacology Group, MTA-ELTE NAP B, Budapest, Hungary            
            János Szentágothai Doctoral School of Neurosciences, Semmelweis University, Budapest, Hungary            
            Stem Cell Research Group, Institute of Molecular Biosciences, Mahidol University, Nakhon Pathom Bangkok, Thailand            
            First Department of Obstetrics and Gynaecology, Semmelweis University, Budapest, Hungary            
            Department of Veterinary and Animal Science, University of Copenhagen, Denmark            
            Cited By :88            
            Export Date: 9 April 2024            
            Correspondence Address: Dinnyés, A.; BioTalentum LtdHungary; email: Manuscript.Dinnyes@biotalentum.hu
AB  - Neural progenitor cells (NPCs) from human induced pluripotent stem cells (hiPSCs) are frequently induced using 3D culture methodologies however, it is unknown whether spheroid-based (3D) neural induction is actually superior to monolayer (2D) neural induction. Our aim was to compare the efficiency of 2D induction with 3D induction method in their ability to generate NPCs, and subsequently neurons and astrocytes. Neural differentiation was analysed at the protein level qualitatively by immunocytochemistry and quantitatively by flow cytometry for NPC (SOX1, PAX6, NESTIN), neuronal (MAP2, TUBB3), cortical layer (TBR1, CUX1) and glial markers (SOX9, GFAP, AQP4). Electron microscopy demonstrated that both methods resulted in morphologically similar neural rosettes. However, quantification of NPCs derived from 3D neural induction exhibited an increase in the number of PAX6/NESTIN double positive cells and the derived neurons exhibited longer neurites. In contrast, 2D neural induction resulted in more SOX1 positive cells. While 2D monolayer induction resulted in slightly less mature neurons, at an early stage of differentiation, the patch clamp analysis failed to reveal any significant differences between the electrophysiological properties between the two induction methods. In conclusion, 3D neural induction increases the yield of PAX6(+)/NESTIN(+) cells and gives rise to neurons with longer neurites, which might be an advantage for the production of forebrain cortical neurons, highlighting the potential of 3D neural induction, independent of iPSCs' genetic background.
LA  - English
DB  - MTMT
ER  - 

TY  - JOUR
AU  - Qi, Y
AU  - Zhang, XJ
AU  - Renier, N
AU  - Wu, Z
AU  - Atkin, T
AU  - Sun, Z
AU  - Ozair, MZ
AU  - Tchieu, J
AU  - Zimmer, B
AU  - Fattahi, F
AU  - Ganat, Y
AU  - Azevedo, R
AU  - Zeltner, N
AU  - Brivanlou, Ali H.
AU  - Karayiorgou, M
AU  - Gogos, J
AU  - Tomishima, M
AU  - Tessier-Lavigne, M
AU  - Shi, SH
AU  - Studer, L
TI  - Combined small-molecule inhibition accelerates the derivation of functional cortical neurons from human pluripotent stem cells.
JF  - NATURE BIOTECHNOLOGY
J2  - NAT BIOTECHNOL
VL  - 35
PY  - 2017
IS  - 2
SP  - 154
EP  - 163
PG  - 10
SN  - 1087-0156
DO  - 10.1038/nbt.3777
UR  - https://m2.mtmt.hu/api/publication/3250780
ID  - 3250780
AB  - Considerable progress has been made in converting human pluripotent stem cells (hPSCs) into functional neurons. However, the protracted timing of human neuron specification and functional maturation remains a key challenge that hampers the routine application of hPSC-derived lineages in disease modeling and regenerative medicine. Using a combinatorial small-molecule screen, we previously identified conditions to rapidly differentiate hPSCs into peripheral sensory neurons. Here we generalize the approach to central nervous system (CNS) fates by developing a small-molecule approach for accelerated induction of early-born cortical neurons. Combinatorial application of six pathway inhibitors induces post-mitotic cortical neurons with functional electrophysiological properties by day 16 of differentiation, in the absence of glial cell co-culture. The resulting neurons, transplanted at 8 d of differentiation into the postnatal mouse cortex, are functional and establish long-distance projections, as shown using iDISCO whole-brain imaging. Accelerated differentiation into cortical neuron fates should facilitate hPSC-based strategies for disease modeling and cell therapy in CNS disorders.
LA  - English
DB  - MTMT
ER  -