TY - JOUR AU - Chandrasekaran, A AU - Avci, HX AU - Ochalek, A AU - Rosingh, LN AU - Molnár, Kinga AU - László, Lajos AU - Bellák, Tamás AU - Teglasi, A AU - Pesti, Krisztina AU - Mike, Árpád AU - Phanthong, P AU - Biró, Orsolya AU - Hall, V AU - Kitiyanant, N AU - Krause, KH AU - Kobolák, Julianna AU - Dinnyés, András TI - Comparison of 2D and 3D neural induction methods for the generation of neural progenitor cells from human induced pluripotent stem cells JF - STEM CELL RESEARCH J2 - STEM CELL RES VL - 25 PY - 2017 SP - 139 EP - 151 PG - 13 SN - 1873-5061 DO - 10.1016/j.scr.2017.10.010 UR - https://m2.mtmt.hu/api/publication/3312483 ID - 3312483 N1 - BioTalentum Ltd, Gödöllő, Hungary Department of Anatomy, Embryology and Histology, Faculty of Medicine, University of Szeged, Szeged, Hungary Molecular Animal Biotechnology Lab, Szent István University, Gödöllő, Hungary Department of Pathology and Immunology, University of Geneva Medical School, Geneva, Switzerland Department of Anatomy, Cell and Developmental Biology, Eötvös Loránd University, Budapest, Hungary Opto-Neuropharmacology Group, MTA-ELTE NAP B, Budapest, Hungary János Szentágothai Doctoral School of Neurosciences, Semmelweis University, Budapest, Hungary Stem Cell Research Group, Institute of Molecular Biosciences, Mahidol University, Nakhon Pathom Bangkok, Thailand First Department of Obstetrics and Gynaecology, Semmelweis University, Budapest, Hungary Department of Veterinary and Animal Science, University of Copenhagen, Denmark Cited By :88 Export Date: 9 April 2024 Correspondence Address: Dinnyés, A.; BioTalentum LtdHungary; email: Manuscript.Dinnyes@biotalentum.hu AB - Neural progenitor cells (NPCs) from human induced pluripotent stem cells (hiPSCs) are frequently induced using 3D culture methodologies however, it is unknown whether spheroid-based (3D) neural induction is actually superior to monolayer (2D) neural induction. Our aim was to compare the efficiency of 2D induction with 3D induction method in their ability to generate NPCs, and subsequently neurons and astrocytes. Neural differentiation was analysed at the protein level qualitatively by immunocytochemistry and quantitatively by flow cytometry for NPC (SOX1, PAX6, NESTIN), neuronal (MAP2, TUBB3), cortical layer (TBR1, CUX1) and glial markers (SOX9, GFAP, AQP4). Electron microscopy demonstrated that both methods resulted in morphologically similar neural rosettes. However, quantification of NPCs derived from 3D neural induction exhibited an increase in the number of PAX6/NESTIN double positive cells and the derived neurons exhibited longer neurites. In contrast, 2D neural induction resulted in more SOX1 positive cells. While 2D monolayer induction resulted in slightly less mature neurons, at an early stage of differentiation, the patch clamp analysis failed to reveal any significant differences between the electrophysiological properties between the two induction methods. In conclusion, 3D neural induction increases the yield of PAX6(+)/NESTIN(+) cells and gives rise to neurons with longer neurites, which might be an advantage for the production of forebrain cortical neurons, highlighting the potential of 3D neural induction, independent of iPSCs' genetic background. LA - English DB - MTMT ER - TY - JOUR AU - Mariani, J AU - Simonini, MV AU - Palejev, D AU - Tomasini, L AU - Coppola, G AU - Szekely, AM AU - Horváth, Tamás AU - Vaccarino, FM TI - Modeling human cortical development in vitro using induced pluripotent stem cells JF - PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA J2 - P NATL ACAD SCI USA VL - 109 PY - 2012 IS - 31 SP - 12770 EP - 12775 PG - 6 SN - 0027-8424 DO - 10.1073/pnas.1202944109 UR - https://m2.mtmt.hu/api/publication/3198991 ID - 3198991 LA - English DB - MTMT ER - TY - JOUR AU - Entezam, Ali AU - Biacsi, Rea Erika AU - Orrison, Bonnie AU - Saha, Tapas AU - Hoffman, Gloria E AU - Grabczyk, Ed AU - Nussbaum, Robert L AU - Usdin, Karen TI - Regional FMRP deficits and large repeat expansions into the full mutation range in a new Fragile X premutation mouse model. JF - GENE J2 - GENE VL - 395 PY - 2007 IS - 1-2 SP - 125 EP - 134 PG - 10 SN - 0378-1119 DO - 10.1016/j.gene.2007.02.026 UR - https://m2.mtmt.hu/api/publication/33090120 ID - 33090120 N1 - Journal Article; Research Support, N.I.H., Intramural AB - Carriers of FMR1 alleles with 55-200 repeats in the 5' UTR are at risk for Fragile X associated tremor and ataxia syndrome. The cause of the neuropathology is unknown but is thought to be RNA-mediated. Maternally transmitted premutation alleles are also at risk of expansion of the repeat tract into the "full mutation" range (>200 repeats). The mechanism responsible for expansion is unknown. Full mutation alleles produce reduced amounts of the FMR1 gene product, FMRP, which leads to Fragile X mental retardation syndrome. We have developed a murine model for Fragile X premutation carriers that recapitulates key features seen in humans including a direct relationship between repeat number and Fmr1 mRNA levels, an inverse relationship with FMRP levels and Purkinje cell dropout that have not been seen in a previously described knock-in mouse model. In addition, these mice also show a differential deficit of FMRP in different parts of the brain that might account for symptoms of the full mutation that are seen in premutation carriers. As in humans, repeat instability is high with expansions predominating and, for the first time in a mouse model, large expansions into the full mutation range are seen that occur within a single generation. Thus, contrary to what was previously thought, mice may be good models not only for the symptoms seen in human carriers of FMR1 premutation alleles but also for understanding the mechanism responsible for repeat expansion, a phenomenon that is responsible for a number of neurological and neurodevelopmental disorders. LA - English DB - MTMT ER -