@article{MTMT:2936910, title = {Generating Marker-Free Transgenic Wheat Using Minimal Gene Cassette and Cold-Inducible Cre/Lox System}, url = {https://m2.mtmt.hu/api/publication/2936910}, author = {Mészáros, Klára and Éva, Csaba and Kiss, Tibor and Bányai, Judit and Kiss, E and Téglás, F and Láng, László and Karsai, Ildikó and Tamás, László}, doi = {10.1007/s11105-014-0830-1}, journal-iso = {PLANT MOL BIOL REP}, journal = {PLANT MOLECULAR BIOLOGY REPORTER}, volume = {33}, unique-id = {2936910}, issn = {0735-9640}, abstract = {The precise elimination of selectable marker genes is highly desirable, when their function is no longer needed, because their presence raised worldwide public concerns against the release of genetically modified plants. This is the first report of simultaneous application of the minimal gene cassette and cold-inducible Cre/lox recombination system in wheat. The bar selection and cre-recombinase genes were eliminated from T0 and T1 transgenic lines with 44 and 51 % efficiency. This approach provides a new, reasonably effective technique to produce selection gene-free transgenic wheat lines either immediately after tissue culture, or from the subsequent transgenic generation. The advantage of this method is that it does not require any additional cold treatment during the plant regeneration/growing because the transgene elimination is ensured by the vernalisation. Application of this method prevents gene flow by pollen and seed, because the selection and recombinase genes are eliminated before pollen development, therefore reducing the risk of GM plants. © 2014, Springer Science+Business Media New York.}, keywords = {WHEAT; Triticum aestivum; SITE-SPECIFIC RECOMBINATION; Selectable marker gene; Monocot; Cold induction; Auto-excision strategy}, year = {2015}, eissn = {1572-9818}, pages = {1221-1231}, orcid-numbers = {Tamás, László/0000-0002-3084-5851} } @article{MTMT:2500830, title = {Phenotyping shows improved physiological traits and seed yield of transgenic wheat plants expressing the alfalfa aldose reductase under permanent drought stress}, url = {https://m2.mtmt.hu/api/publication/2500830}, author = {Fehér-Juhász, Erzsébet and Majer, P and Sass, László and Lantos, Csaba and Csiszár, Jolán and Túróczy, Zoltán and Mihály, Róbert and Mai, Antal and Horváth V., Gábor and Vass, Imre and Dudits, Dénes and Pauk, János}, doi = {10.1007/s11738-013-1445-0}, journal-iso = {ACTA PHYSIOL PLANT}, journal = {ACTA PHYSIOLOGIAE PLANTARUM}, volume = {36}, unique-id = {2500830}, issn = {0137-5881}, abstract = {Members of the aldo-keto reductase family including aldose reductases are involved in antioxidant defense by metabolizing a wide range of lipid peroxidation-derived cytotoxic compounds. Therefore, we produced transgenic wheat genotypes over-expressing the cDNA of alfalfa aldose reductase gene. These plants consequently exhibit 1.5-4.3 times higher detoxification activity for the aldehyde substrate. Permanent drought stress was generated in the greenhouse by growing wheat plants in soil with 20 % water capacity. The control and stressed plants were monitored by a semi automatic phenotyping platform providing computer-controlled watering, digital and thermal imaging. Calculation of biomass values was based on the correlation (R2 = 0.7556) between fresh weight and green pixel-based shoot surface area. The green biomass production by plants of the three transgenic lines was 12-26-41 % higher than the non-transgenic plants' grown under water limitation. Thermal imaging of stressed non-transgenic plants indicated an elevation in the leaf temperature. The thermal status of transformants was similar at both normal and suboptimal water regime. In drought, the transgenic plants used more water during the growing season. The described phenotyping platform provided a comprehensive data set demonstrating the improved physiological condition of the drought stressed transgenic wheat plants in the vegetative growth phase. In soil with reduced water capacity two transgenic genotypes showed higher seed weight per plant than the control non-transgenic one. Limitation of greenhouse-based phenotyping in analysis of yield potential is discussed. © 2013 The Author(s).}, keywords = {TRANSFORMATION; BIOMASS; seed production; Water usage; Aldo-keto reductase; Digital and thermal imaging}, year = {2014}, eissn = {1861-1664}, pages = {663-673}, orcid-numbers = {Csiszár, Jolán/0000-0001-7250-3000} } @article{MTMT:2757477, title = {Sulphur starvation induces the expression of microRNA-395 and one of its target genes but in different cell types}, url = {https://m2.mtmt.hu/api/publication/2757477}, author = {Kawashima, Cintia Goulart and Yoshimoto, Naoko and Maruyama-Nakashita, Akiko and Tsuchiya, Yumiko N and Saito, Kazuki and Takahashi, Hideki and Dalmay, Tamas}, doi = {10.1111/j.1365-313X.2008.03690.x}, journal-iso = {PLANT J}, journal = {PLANT JOURNAL}, volume = {57}, unique-id = {2757477}, issn = {0960-7412}, year = {2009}, eissn = {1365-313X}, pages = {313-321} } @article{MTMT:1236871, title = {Transgenic approach to improve wheat (Triticum aestivum L) nutritional quality.}, url = {https://m2.mtmt.hu/api/publication/1236871}, author = {Tamás, Cecília and Némethné Kisgyörgy, Boglárka and Rakszegi, Marianna and Wilkinson, MD and Yang, MS and Láng, László and Tamás, László and Bedő, Zoltán}, doi = {10.1007/s00299-009-0716-0}, journal-iso = {PLANT CELL REP}, journal = {PLANT CELL REPORTS}, volume = {28}, unique-id = {1236871}, issn = {0721-7714}, year = {2009}, eissn = {1432-203X}, pages = {1085-1094}, orcid-numbers = {Tamás, László/0000-0002-3084-5851} } @article{MTMT:1108486, title = {The effect of drought and heat stress on reproductive processes in cereals.}, url = {https://m2.mtmt.hu/api/publication/1108486}, author = {Barnabás, Beáta and Jäger, Katalin and Fehér, Attila}, doi = {10.1111/j.1365-3040.2007.01727.x}, journal-iso = {PLANT CELL ENVIRON}, journal = {PLANT CELL AND ENVIRONMENT}, volume = {31}, unique-id = {1108486}, issn = {0140-7791}, year = {2008}, eissn = {1365-3040}, pages = {11-38}, orcid-numbers = {Fehér, Attila/0000-0002-4183-3696} } @article{MTMT:1138147, title = {Development and characterization of a chimaeric tissue-specific promoter in wheat and rice endosperm}, url = {https://m2.mtmt.hu/api/publication/1138147}, author = {Oszvald, Mária and Gárdonyi, Márk and Tamás, Cecília and Takács, Imre and Jenes, Barnabás and Tamás, László}, doi = {10.1007/s11627-007-9082-1}, journal-iso = {IN VITRO CELL DEV-PL}, journal = {IN VITRO CELLULAR AND DEVELOPMENTAL BIOLOGY-PLANT}, volume = {44}, unique-id = {1138147}, issn = {1054-5476}, abstract = {The recently achieved significant improvement of cereal transformation protocols provides facilities to alter the protein composition of the endosperm, for example, to increase or decrease the quantity of one of its protein components or to express foreign molecules. To achieve this goal, strong endosperm-specific promoters have to be available. The aim of our work was to develop a more efficient tissue-specific promoter which is currently used. A chimaeric promoter was assembled using the 5' UTR (1,900 bp) of the gene coding for the 1Bx17 HMW glutenin subunit protein, responsible for tissue-specific expression and the first intron of the rice actin gene (act1). The sequence around of the translation initial codon was optimized. The effect of the intron and promoter regulatory sequences, using different lengths of 1Bx17 HMW-GS promoter, were studied on the expression of uidA gene. The function of promoter elements, promoter length, and the first intron of the rice actin gene were tested by a transient expression assay in immature wheat endosperm and in stable transgenic rice plants. Results showed that insertion of the rice act1 first intron increased GUS expression by four times in transient assay. The shortest 1Bx17 HMW-GS promoter fragment (173 bp) linked to the intron and GUS reporter gene provided almost the same expression level than the intronless long 1Bx17 HMW-GS promoter. Analysis of the stable transformant plants revealed that 173 nucleotides were sufficient for endosperm-specific expression of the uidA gene, despite 13 nucleotides missing from the HMW enhancer sequence, a relevant regulatory element in the promoter region.}, year = {2008}, eissn = {1475-2689}, pages = {1-7}, orcid-numbers = {Tamás, László/0000-0002-3084-5851} } @article{MTMT:1129204, title = {Technological quality of field grown transgenic lines of commercial wheat cultivars expressing the 1Ax1 HMW glutenin subunit gene}, url = {https://m2.mtmt.hu/api/publication/1129204}, author = {Rakszegi, Marianna and Pastori, G and Jones, HD and Békés, Ferenc and Butow, B and Láng, László and Bedő, Zoltán and Shewry, PR}, doi = {10.1016/j.jcs.2007.04.010}, journal-iso = {J CEREAL SCI}, journal = {JOURNAL OF CEREAL SCIENCE}, volume = {47}, unique-id = {1129204}, issn = {0733-5210}, abstract = {Ten transgenic lines were studied which expressed a transgene encoding HMW subunit 1Ax1 in three elite spring wheat cultivars: Imp, Canon and Cadenza. These lines contained one to five copies of the transgene and the 1Ax1 subunit was expressed as 1-20% of the total glutenin protein. These lines were grown in field trials in a continental, and climate (Martonvasar, Hungary) over two years (2004, 2005). The expression of the transgenes and their effects on the grain properties were stably inherited over the two years. Significant differences in yield were observed between three of the transgenic lines and the original genotypes, but no differences were found in their adaptiveness. Clear differences were found in the technological and rheological properties of four lines, with all the parameters characterising dough strength and extensibility (GI, W, G, Re, Ext, A) changing significantly. These differences were associated with increases in the ratio of HMW/LMW subunits and decreases in the ratios of 1Dx/1 Dy and 1Bx/1By subunits. Two transgenic lines of cv Imp had high over-expression of the 1Ax1 subunit which in one line resulted in an overstrong type of dough, similar to that described previously for lines over-expressing HMW subunit 1Dx5. Transformation of cvs. Canon and Cadenza resulted in two lines with increased dough stability due to the significantly improved gluten quality. It is concluded that significant changes in the structure of the glutenin polymers caused by the altered ratio of x-type to y-type HMW subunits led to the changes in flour functional properties. (c) 2007 Elsevier Ltd. All rights reserved.}, keywords = {PERFORMANCE LIQUID-CHROMATOGRAPHY; FUNCTIONAL-PROPERTIES; AGRONOMIC PERFORMANCE; WEIGHT; BREAD WHEAT; MIXING PROPERTIES; GRAIN DEVELOPMENT; BREADMAKING QUALITY; FLOUR PROTEINS; HEAT-STRESS}, year = {2008}, eissn = {1095-9963}, pages = {310-321} } @article{MTMT:1242928, title = {Comparative field performance over 3 years and two sites of transgenic wheat lines expressing hmw subunit transgenes}, url = {https://m2.mtmt.hu/api/publication/1242928}, author = {Shewry, PR and Powers, S and Field, JM and Fido, RJ and Jones, HD and Arnold, GM and West, J and Lazzeri, PA and Barcelo, P and Barro, F and Tatham, AS and Békés, Ferenc and Butow, B and Darlington, H}, doi = {10.1007/s00122-006-0279-1}, journal-iso = {THEOR APPL GENET}, journal = {THEORETICAL AND APPLIED GENETICS}, volume = {113}, unique-id = {1242928}, issn = {0040-5752}, year = {2006}, eissn = {1432-2242}, pages = {128-136} } @article{MTMT:1129185, title = {Technological quality of transgenic wheat expressing an increased amount of a HMW glutenin subunit}, url = {https://m2.mtmt.hu/api/publication/1129185}, author = {Rakszegi, Marianna and Békés, Ferenc and Láng, László and Tamás, László and Shewry, PR and Bedő, Zoltán}, doi = {10.1016/j.jcs.2005.02.006}, journal-iso = {J CEREAL SCI}, journal = {JOURNAL OF CEREAL SCIENCE}, volume = {42}, unique-id = {1129185}, issn = {0733-5210}, abstract = {The transgenic line B73-6-1 (developed and characterized by UK scientists) contains 10-15 extra copies of a native HMW glutenin gene (1Dx5) with an approximately four-fold increase in the amount of the encoded protein. The technological and rheological properties of this line and the non-transformed control line were studied in a continental type, and climatic environment (Martonvasar, Hungary, 2000-2002). The results show that in the three years of the field experiment, the transgene and associated functional properties were stably inherited over several generations. No difference was found in yield between the transgenic line and the original genotype, but clear genotypic differences were found in kernel hardness and kernel size, The transgenic line had an increased kernel hardness and a smaller kernel size. The transgenic line B73-6-1 tended to have a higher protein content than the control L88-6, but this difference was not statistically significant. In contrast there was a significant, 400%, increase in the amount of 1Dx5 HMW glutenin subunits, Dx/Dy-, HMW/LMW- and glutenin/gliadin ratio. At the same time there was a decrease in wet gluten content and SDS sedimentation. Thus, significant changes in the structure of the protein matrix caused by the altered x to y ratio of HMW-GS and as a result of this alteration, flour functional properties changed. Parameters that characterize the stability and strength of the dough confirmed that B73-6-1 was stronger, but the extensibility was reduced. This flour would be suitable for blending with flour of lower grade. (c) 2005 Elsevier Ltd. All rights reserved.}, keywords = {PERFORMANCE LIQUID-CHROMATOGRAPHY; FUNCTIONAL-PROPERTIES; SIZE; rheology; DEPOLYMERIZATION; MACROPOLYMER; DOUGH; AGRONOMIC PERFORMANCE; MIXING PROPERTIES; FLOUR PROTEINS}, year = {2005}, eissn = {1095-9963}, pages = {15-23}, orcid-numbers = {Tamás, László/0000-0002-3084-5851} } @article{MTMT:1242926, title = {Modification of the low molecular weight (lmw) glutenin composition of transgenic durum wheat: effects on glutenin polymer size and gluten functionality}, url = {https://m2.mtmt.hu/api/publication/1242926}, author = {Tosi, P and Masci, S and Giovangrossi, A and D, Ovidio R and Békés, Ferenc and Larroque, O and Napier, J and Shewry, P}, doi = {10.1007/s11032-005-5912-1}, journal-iso = {MOL BREEDING}, journal = {MOLECULAR BREEDING}, volume = {16}, unique-id = {1242926}, issn = {1380-3743}, year = {2005}, eissn = {1572-9788}, pages = {113-126} } @article{MTMT:1129177, title = {Dissemination of the highly expressed Bx7 glutenin subunit (Glu-B1al allele) in wheat as revealed by novel PCR markers and RP-HPLC}, url = {https://m2.mtmt.hu/api/publication/1129177}, author = {Butow, BJ and Gale, KR and Ikea, J and Juhász, Angéla and Bedő, Zoltán and Tamás, László and Gianibelli, MC}, doi = {10.1007/s00122-004-1776-8}, journal-iso = {THEOR APPL GENET}, journal = {THEORETICAL AND APPLIED GENETICS}, volume = {109}, unique-id = {1129177}, issn = {0040-5752}, abstract = {Increased expression of the high molecular weight glutenin subunit (HMW-GS) Bx7 is associated with improved dough strength of wheat (Triticum aestivum L.) flour. Several cultivars and landraces of widely different genetic backgrounds from around the world have now been found to contain this so-called 'over-expressing' allelic form of the Bx7 subunit encoded by Glu-B1al. Using three methods of identification, SDS-PAGE, RP-HPLC and PCR marker analysis, as well as pedigree information, we have traced the distribution and source of this allele from a Uruguayan landrace, Americano 44D, in the mid-nineteenth century. Results are supported by knowledge of the movement of wheat lines with migrants. All cultivars possessing the Glu-B1al allele can be identified by the following attributes: (1) the elution of the By sub-unit peak before the Dx sub-unit peak by RP-HPLC, (2) high expression levels of Bx7 (>39% Mol% Bx), (3) a 43 bp insertion in the matrix-attachment region (MAR) upstream of the gene promoter relative to Bx7 and an 18 bp nucleotide duplication in the coding region of the gene. Evidence is presented indicating that these 18 and 43 bp sequence insertions are not causal for the high expression levels of Bx7 as they were also found to be present in a small number of hexaploid species, including Chinese Spring, and species expressing Glu-B1ak and Glu-B1a alleles. In addition, these sequence inserts were found in different isolates of the tetraploid wheat, T. turgidum, indicating that these insertion/deletion events occurred prior to hexaploidization.}, keywords = {PROTEIN; LOCI; PROLAMIN; DICOCCOIDES; DOUGH STRENGTH; GENETIC-CONTROL; QUANTITATIVE VARIATION; BREAD-MAKING QUALITY}, year = {2004}, eissn = {1432-2242}, pages = {1525-1535}, orcid-numbers = {Tamás, László/0000-0002-3084-5851} } @article{MTMT:1242922, title = {Expression of epitope-tagged lmw glutenin subunits in the starchy endosperm of transgenic wheat and their incorporation into glutenin polymers}, url = {https://m2.mtmt.hu/api/publication/1242922}, author = {Tosi, P and D, Ovidio R and Napier, JA and Békés, Ferenc and Shewry, PR}, doi = {10.1007/s00122-003-1459-x}, journal-iso = {THEOR APPL GENET}, journal = {THEORETICAL AND APPLIED GENETICS}, volume = {108}, unique-id = {1242922}, issn = {0040-5752}, year = {2004}, eissn = {1432-2242}, pages = {468-476} } @article{MTMT:4934, title = {Bánkúti 1201 - an old Hungarian wheat variety with special storage protein composition}, url = {https://m2.mtmt.hu/api/publication/4934}, author = {Juhász, Angéla and Larroque, OR and Tamás, László and Hsam, Slk and Zeller, FJ and Békés, Ferenc and Bedő, Zoltán}, doi = {10.1007/s00122-003-1292-2}, journal-iso = {THEOR APPL GENET}, journal = {THEORETICAL AND APPLIED GENETICS}, volume = {107}, unique-id = {4934}, issn = {0040-5752}, year = {2003}, eissn = {1432-2242}, pages = {697-704}, orcid-numbers = {Tamás, László/0000-0002-3084-5851} } @article{MTMT:1218499, title = {Positive selectable marker genes for routine plant transformation}, url = {https://m2.mtmt.hu/api/publication/1218499}, author = {Penna, S and Sági, László and Swennen, R}, doi = {10.1079/IVP2001272}, journal-iso = {IN VITRO CELL DEV-PL}, journal = {IN VITRO CELLULAR AND DEVELOPMENTAL BIOLOGY-PLANT}, volume = {38}, unique-id = {1218499}, issn = {1054-5476}, year = {2002}, eissn = {1475-2689}, pages = {125-128} } @article{MTMT:4926, title = {Current Status of Wheat Transformation}, url = {https://m2.mtmt.hu/api/publication/4926}, author = {Rakszegi, Marianna and Tamás, Cecília and Szűcs, Péter and Tamás, László and Bedő, Zoltán}, journal-iso = {J PLANT BIOTECHNOL}, journal = {JOURNAL OF PLANT BIOTECHNOLOGY}, volume = {3}, unique-id = {4926}, issn = {1229-2818}, year = {2001}, pages = {67-81}, orcid-numbers = {Tamás, László/0000-0002-3084-5851} } @article{MTMT:1910668, title = {Meristem and cell division and S-phase-dependent activity of wheat histone H4 promoter in transgenic maize plants}, url = {https://m2.mtmt.hu/api/publication/1910668}, author = {Bilgin, Metin and Dedeoglu, Damla and OMIRULLEH, S and Peres, Adrián and ENGLER, G and INZÉ, D and Dudits, Dénes and Fehér, Attila}, doi = {10.1016/S0168-9452(99)00005-9}, journal-iso = {PLANT SCI}, journal = {PLANT SCIENCE}, volume = {143}, unique-id = {1910668}, issn = {0168-9452}, year = {1999}, eissn = {1873-2259}, pages = {35-44}, orcid-numbers = {Fehér, Attila/0000-0002-4183-3696} } @article{MTMT:1242892, title = {Transformation of pasta wheat (triticum turgidum l-var. durum) with high-molecular-weight glutenin subunit genes and modification of dough functionality}, url = {https://m2.mtmt.hu/api/publication/1242892}, author = {He, GY and Rooke, L and Steele, S and Békés, Ferenc and Gras, P and Tatham, AS and Fido, R and Barcelo, P and Shewry, PR and Lazzeri, PA}, doi = {10.1023/A:1009681321708}, journal-iso = {MOL BREEDING}, journal = {MOLECULAR BREEDING}, volume = {5}, unique-id = {1242892}, issn = {1380-3743}, year = {1999}, eissn = {1572-9788}, pages = {377-386} } @article{MTMT:1242889, title = {Overexpression of a gluten protein in transgenic wheat results in greatly increased dough strength}, url = {https://m2.mtmt.hu/api/publication/1242889}, author = {Rooke, L and Békés, Ferenc and Fido, R and Barro, F and Gras, P and Tatham, AS and Barcelo, P and Lazzeri, P and Shewry, PR}, doi = {10.1006/jcrs.1999.0265}, journal-iso = {J CEREAL SCI}, journal = {JOURNAL OF CEREAL SCIENCE}, volume = {30}, unique-id = {1242889}, issn = {0733-5210}, year = {1999}, eissn = {1095-9963}, pages = {115-120} } @article{MTMT:4850, title = {Breeding for breadmaking quality using old Hungarian wheat varieties (Reprinted from Wheat: Prospects for global improvement, 1998)}, url = {https://m2.mtmt.hu/api/publication/4850}, author = {Bedő, Zoltán and Vida, Gyula and Láng, László and Karsai, Ildikó}, doi = {10.1023/A:1018373805161}, journal-iso = {EUPHYTICA}, journal = {EUPHYTICA}, volume = {100}, unique-id = {4850}, issn = {0014-2336}, year = {1998}, eissn = {1573-5060}, pages = {179-182} } @article{MTMT:1901982, title = {Identification of DNA sequences required for activity of the cauliflower mosaic virus 35S promoter.}, url = {https://m2.mtmt.hu/api/publication/1901982}, author = {ODELL, JT and Nagy, Ferenc István and CHUA, NH}, doi = {10.1038/313810a0}, journal-iso = {NATURE}, journal = {NATURE}, volume = {313}, unique-id = {1901982}, issn = {0028-0836}, year = {1985}, eissn = {1476-4687}, pages = {810-812}, orcid-numbers = {Nagy, Ferenc István/0000-0002-6157-9269} } @article{MTMT:1103325, title = {Transcription of Cauliflower Mosaic-virus Dna - Detection of Promoter Sequences, And Characterization of Transcripts}, url = {https://m2.mtmt.hu/api/publication/1103325}, author = {Guilley, H and Dudley, RK and Jonard, G and Balázs, Ervin and Richards, KE}, doi = {10.1016/0092-8674(82)90281-1}, journal-iso = {CELL}, journal = {CELL}, volume = {30}, unique-id = {1103325}, issn = {0092-8674}, year = {1982}, eissn = {1097-4172}, pages = {763-773} } @article{MTMT:1903385, title = {Study of callus growth and organ formation in wheat tissue cultures.}, url = {https://m2.mtmt.hu/api/publication/1903385}, author = {Dudits, Dénes and Németh, Gábor and Haydu, Zsolt}, journal-iso = {CAN J BOT}, journal = {CANADIAN JOURNAL OF BOTANY-REVUE CANADIENNE DE BOTANIQUE}, volume = {53}, unique-id = {1903385}, issn = {0008-4026}, year = {1975}, eissn = {1480-3305}, pages = {957-963} }