TY - JOUR AU - Kari, Beáta AU - Csordás, Gábor AU - Honti, Viktor AU - Cinege, Gyöngyi Ilona AU - Williams, MJ AU - Andó, István AU - Kurucz, Judit Éva TI - The raspberry Gene Is Involved in the Regulation of the Cellular Immune Response in Drosophila melanogaster JF - PLOS ONE J2 - PLOS ONE VL - 11 PY - 2016 IS - 3 PG - 13 SN - 1932-6203 DO - 10.1371/journal.pone.0150910 UR - https://m2.mtmt.hu/api/publication/3045263 ID - 3045263 N1 - Biological Research Centre of Hungarian Academy of Sciences, Immunology Unit, Institute of Genetics, Szeged, Hungary Department of Neuroscience, Functional Pharmacology, Uppsala University, Uppsala, Sweden Cited By :6 Export Date: 14 February 2021 CODEN: POLNC AB - Drosophila is an extremely useful model organism for understanding how innate immune mechanisms defend against microbes and parasitoids. Large foreign objects trigger a potent cellular immune response in Drosophila larva. In the case of endoparasitoid wasp eggs, this response includes hemocyte proliferation, lamellocyte differentiation and eventual encapsulation of the egg. The encapsulation reaction involves the attachment and spreading of hemocytes around the egg, which requires cytoskeletal rearrangements, changes in adhesion properties and cell shape, as well as melanization of the capsule. Guanine nucleotide metabolism has an essential role in the regulation of pathways necessary for this encapsulation response. Here, we show that the Drosophila inosine 5'-monophosphate dehydrogenase (IMPDH), encoded by raspberry (ras), is centrally important for a proper cellular immune response against eggs from the parasitoid wasp Leptopilina boulardi. Notably, hemocyte attachment to the egg and subsequent melanization of the capsule are deficient in hypomorphic ras mutant larvae, which results in a compromised cellular immune response and increased survival of the parasitoid. LA - English DB - MTMT ER - TY - JOUR AU - Bretscher, AJ AU - Honti, Viktor AU - Binggeli, O AU - Burri, O AU - Poidevin, M AU - Kurucz, Judit Éva AU - Zsámboki, János AU - Andó, István AU - Lemaitre, B TI - The Nimrod transmembrane receptor Eater is required for hemocyte attachment to the sessile compartment in Drosophila melanogaster. JF - BIOLOGY OPEN J2 - BIOL OPEN VL - 4 PY - 2015 IS - 3 SP - 355 EP - 363 PG - 9 SN - 2046-6390 DO - 10.1242/bio.201410595 UR - https://m2.mtmt.hu/api/publication/2853633 ID - 2853633 AB - Eater is an EGF-like repeat transmembrane receptor of the Nimrod family and is expressed in Drosophila hemocytes. Eater was initially identified for its role in phagocytosis of both Gram-positive and Gram-negative bacteria. We have deleted eater and show that it appears to be required for efficient phagocytosis of Gram-positive but not Gram-negative bacteria. However, the most striking phenotype of eater deficient larvae is the near absence of sessile hemocytes, both plasmatocyte and crystal cell types. The eater deletion is the first loss of function mutation identified that causes absence of the sessile hemocyte state. Our study shows that Eater is required cell-autonomously in plasmatocytes for sessility. However, the presence of crystal cells in the sessile compartment requires Eater in plasmatocytes. We also show that eater deficient hemocytes exhibit a cell adhesion defect. Collectively, our data uncovers a new requirement of Eater in enabling hemocyte attachment at the sessile compartment and points to a possible role of Nimrod family members in hemocyte adhesion. LA - English DB - MTMT ER - TY - JOUR AU - Márkus, Róbert AU - Laurinyecz, Barbara AU - Kurucz, Judit Éva AU - Honti, Viktor AU - Bajusz, Izabella AU - Sipos, Botond AU - Somogyi, Kálmán AU - Kronhamn, J AU - Hultmark, D AU - Andó, István TI - Sessile hemocytes as a hematopoietic compartment in drosophila melanogaster JF - PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA J2 - P NATL ACAD SCI USA VL - 106 PY - 2009 IS - 12 SP - 4805 EP - 4809 PG - 5 SN - 0027-8424 DO - 10.1073/pnas.0801766106 UR - https://m2.mtmt.hu/api/publication/1920757 ID - 1920757 N1 - Cited By :174 Export Date: 30 June 2022 CODEN: PNASA LA - English DB - MTMT ER - TY - JOUR AU - Kurucz, Judit Éva AU - Váczi, Balázs AU - Márkus, Róbert AU - Laurinyecz, Barbara AU - Vilmos, Péter AU - Zsámboki, János AU - Csorba, Kinga AU - Gateff, E AU - Hultmark, D AU - Andó, István TI - Definition of Drosophila hemocyte subsets by cell-type specific antigens JF - ACTA BIOLOGICA HUNGARICA (1983-2018) J2 - ACTA BIOL HUNG VL - 58 PY - 2007 IS - Suppl. 1 SP - 95 EP - 111 PG - 17 SN - 0236-5383 DO - 10.1556/ABiol.58.2007.Suppl.8 UR - https://m2.mtmt.hu/api/publication/1915261 ID - 1915261 AB - We analyzed the heterogeneity of Drosophila hemocytes on the basis of the expression of cell-type specific antigens. The antigens characterize distinct subsets which partially overlap with those defined by morphological criteria. Oil the basis of the expression or the lack of expression of blood cell antigens the following hemocyte populations have been defined: crystal cells, plasmalocytes, lamellocytes and precursor cells. The expression of the antigens and thus the different cell types are developmentally regulated. The hemocytes are arranged ill four main compartments: the circulating blood cells, the sessile tissue, the lymph glands and the posterior hematopoietic tissue. Each hemocyte compartment has a specific and characteristic composition of the various cell types. The described markers represent the first successful attempt to define hemocyte lineages by immunological markers in Drosophila and help to define morphologically, functionally, spatially and developmentally distinct subsets of hemocyles. LA - English DB - MTMT ER - TY - JOUR AU - Nehme, NT AU - Liegeois, S AU - Kele, Beatrix AU - Giammarinaro, P AU - Pradel, E AU - Hoffmann, JA AU - Ewbank, JJ AU - Ferrandon, D TI - A model of bacterial intestinal infections in Drosophila melanogaster JF - PLOS PATHOGENS J2 - PLOS PATHOG VL - 3 PY - 2007 IS - 11 PG - 16 SN - 1553-7366 DO - 10.1371/journal.ppat.0030173 UR - https://m2.mtmt.hu/api/publication/2505827 ID - 2505827 N1 - Megjegyzés-22155744 DI: 10.1371/journal.ppat.0030173 AB - Serratia marcescens is an entomopathogenic bacterium that opportunistically infects a wide range of hosts, including humans. In a model of septic injury, if directly introduced into the body cavity of Drosophila, this pathogen is insensitive to the host's systemic immune response and kills flies in a day. We find that S. marcescens resistance to the Drosophila immune deficiency (imd)-mediated humoral response requires the bacterial lipopolysaccharide O-antigen. If ingested by Drosophila, bacteria cross the gut and penetrate the body cavity. During this passage, the bacteria can be observed within the cells of the intestinal epithelium. In such an oral infection model, the flies succumb to infection only after 6 days. We demonstrate that two complementary host defense mechanisms act together against such food-borne infection: an antimicrobial response in the intestine that is regulated by the imd pathway and phagocytosis by hemocytes of bacteria that have escaped into the hemolymph. Interestingly, bacteria present in the hemolymph elicit a systemic immune response only when phagocytosis is blocked. Our observations support a model wherein peptidoglycan fragments released during bacterial growth activate the imd pathway and do not back a proposed role for phagocytosis in the immune activation of the fat body. Thanks to the genetic tools available in both host and pathogen, the molecular dissection of the interactions between S. marcescens and Drosophila will provide a useful paradigm for deciphering intestinal pathogenesis. LA - English DB - MTMT ER - TY - JOUR AU - Rus, Florentina AU - Kurucz, Judit Éva AU - Márkus, Róbert AU - Sinenko, SA AU - Laurinyecz, Barbara AU - Pataki, Andrea Csilla AU - Gausz, János AU - Hegedűs, Zoltán AU - Udvardy, Andor AU - Hultmark, D AU - Andó, István TI - Expression pattern of Filamin-240 in Drosophila blood cells JF - GENE EXPRESSION PATTERNS J2 - GENE EXPR PATTERNS VL - 6 PY - 2006 IS - 8 SP - 928 EP - 934 PG - 7 SN - 1567-133X DO - 10.1016/j.modgep.2006.03.005 UR - https://m2.mtmt.hu/api/publication/1786592 ID - 1786592 N1 - Export Date: 27 January 2024; CODEN: GEPEA LA - English DB - MTMT ER - TY - JOUR AU - Márkus, Róbert AU - Kurucz, Judit Éva AU - Rus, Florentina AU - Andó, István TI - Sterile wounding is a minimal and sufficient trigger for a cellular immune response in Drosophila melanogaster JF - IMMUNOLOGY LETTERS J2 - IMMUNOL LETT VL - 101 PY - 2005 IS - 1 SP - 108 EP - 111 PG - 4 SN - 0165-2478 DO - 10.1016/j.imlet.2005.03.021 UR - https://m2.mtmt.hu/api/publication/1913892 ID - 1913892 LA - English DB - MTMT ER - TY - JOUR AU - Zettervall, CJ AU - Anderl, I AU - Williams, MJ AU - Palmer, R AU - Kurucz, Judit Éva AU - Andó, István AU - Hultmark, D TI - A directed screen for genes involved in Drosophila blood cell activation JF - PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA J2 - P NATL ACAD SCI USA VL - 101 PY - 2004 IS - 39 SP - 14192 EP - 14197 PG - 6 SN - 0027-8424 DO - 10.1073/pnas.0403789101 UR - https://m2.mtmt.hu/api/publication/1913536 ID - 1913536 LA - English DB - MTMT ER - TY - JOUR AU - Kurucz, Judit Éva AU - Zettervall, CJ AU - Sinka, Rita AU - Vilmos, Péter AU - Pivarcsi, A AU - Ekengren, S AU - Hegedűs, Zoltán AU - Andó, István AU - Hultmark, D TI - Hemese, a hemocyte-specific transmembrane protein, affects the cellular immune response in Drosophila JF - PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA J2 - P NATL ACAD SCI USA VL - 100 PY - 2003 IS - 5 SP - 2622 EP - 2627 PG - 6 SN - 0027-8424 DO - 10.1073/pnas.0436940100 UR - https://m2.mtmt.hu/api/publication/1912851 ID - 1912851 LA - English DB - MTMT ER -