TY - JOUR AU - Sághy, Éva AU - Payrits, Maja AU - Biro-Suto, T AU - Skodáné Földes, Rita AU - Szánti-Pintér, Eszter AU - Erostyák, János AU - Makkai, Géza AU - Sétáló, György (ifj.) AU - Kollár, László AU - Kőszegi, Tamás AU - Jakabfi-Csepregi, Rita AU - Szolcsányi, János AU - Helyes, Zsuzsanna AU - Szőke, Éva TI - Carboxamido steroids inhibit the opening properties of Transient Receptor Potential ion channels by lipid raft modulation. JF - JOURNAL OF LIPID RESEARCH J2 - J LIPID RES VL - 59 PY - 2018 IS - 10 SP - 1851 EP - 1863 PG - 13 SN - 0022-2275 DO - 10.1194/jlr.M084723 UR - https://m2.mtmt.hu/api/publication/3402940 ID - 3402940 N1 - * Megosztott szerzőség AB - Transient Receptor Potential (TRP) cation channels, like the TRP Vanilloid 1 and TRP Ankyrin 1 (TRPV1 and TRPA1) are expressed on primary sensory neurons. These thermosensor channels play role in pain processing. We provided evidence that lipid raft disruption influenced the TRP channel activation and a carboxamido-steroid compound (C1) inhibited TRPV1 activation. Therefore, our aim was to investigate whether this compound exerts its effect through lipid raft disruption and the steroid backbone (C3) or altered position of the carboxamido group (C2) influence the inhibitory action by measuring Ca2+-transients on isolated neurons and calcium-uptake on receptor-expressing CHO cells. Membrane cholesterol content was measured by filipin staining and membrane polarisation by fluorescence spectroscopy. Both the percentage of responsive cells and the magnitude of the intracellular Ca2+-enhancement evoked by the TRPV1 agonist capsaicin were significantly inhibited after C1 and C2 incubation, but not after C3 administration. C1 was able to reduce other TRP channel activation as well. The compounds induced cholesterol depletion in CHO cells, but only C1 induced changes in membrane polarisation. The inhibitory action of the compounds on TRP channel activation develops by lipid raft disruption, and the presence and the position of the carboxamido group is essential. LA - English DB - MTMT ER - TY - JOUR AU - Balajthy, András AU - Hajdu, Péter Béla AU - Panyi, György AU - Varga, Zoltán TI - Sterol Regulation of Voltage-Gated K+ Channels JF - CURRENT TOPICS IN MEMBRANES J2 - CURR TOP MEMBR VL - 80 PY - 2017 SP - 255 EP - 292 PG - 38 SN - 1063-5823 DO - 10.1016/bs.ctm.2017.05.006 UR - https://m2.mtmt.hu/api/publication/3272305 ID - 3272305 LA - English DB - MTMT ER - TY - JOUR AU - Kovács, Tamás AU - Batta, Gyula Gábor (Ifj.) AU - Zákány, Florina AU - Szöllősi, János AU - Nagy, Péter TI - The dipole potential correlates with lipid raft markers in the plasma membrane of living cells JF - JOURNAL OF LIPID RESEARCH J2 - J LIPID RES VL - 58 PY - 2017 IS - 8 SP - 1681 EP - 1691 PG - 11 SN - 0022-2275 DO - 10.1194/jlr.M077339 UR - https://m2.mtmt.hu/api/publication/3245960 ID - 3245960 LA - English DB - MTMT ER - TY - JOUR AU - Tomczak, Adam P AU - Fernández-Trillo, Jorge AU - Bharill, Shashank AU - Papp, Ferenc AU - Panyi, György AU - Stühmer, Walter AU - Isacoff, Ehud Y AU - Pardo, Luis A TI - A new mechanism of voltage-dependent gating exposed by KV10.1 channels interrupted between voltage sensor and pore JF - JOURNAL OF GENERAL PHYSIOLOGY J2 - J GEN PHYSIOL VL - 149 PY - 2017 IS - 5 SP - 577 EP - 593 PG - 17 SN - 0022-1295 DO - 10.1085/jgp.201611742 UR - https://m2.mtmt.hu/api/publication/3334522 ID - 3334522 LA - English DB - MTMT ER - TY - JOUR AU - Balajthy, András AU - Somodi, Sándor AU - Pethő, Z AU - Péter, Mária AU - Varga, Zoltán AU - P. Szabó, Gabriella AU - Paragh, György AU - Vigh, László AU - Panyi, György AU - Hajdu, Péter Béla TI - 7DHC-induced changes of Kv1.3 operation contributes to modified T cell function in Smith-Lemli-Opitz syndrome JF - PFLUGERS ARCHIV-EUROPEAN JOURNAL OF PHYSIOLOGY J2 - PFLUG ARCH EUR J PHY VL - 468 PY - 2016 IS - 8 SP - 1403 EP - 1418 PG - 16 SN - 0031-6768 DO - 10.1007/s00424-016-1851-4 UR - https://m2.mtmt.hu/api/publication/3099238 ID - 3099238 AB - In vitro manipulation of membrane sterol level affects the regulation of ion channels and consequently certain cellular functions; however, a comprehensive study that confirms the pathophysiological significance of these results is missing. The malfunction of 7-dehydrocholesterol (7DHC) reductase in Smith-Lemli-Opitz syndrome (SLOS) leads to the elevation of the 7-dehydrocholesterol level in the plasma membrane. T lymphocytes were isolated from SLOS patients to assess the effect of the in vivo altered membrane sterol composition on the operation of the voltage-gated Kv1.3 channel and the ion channel-dependent mitogenic responses. We found that the kinetic and equilibrium parameters of Kv1.3 activation changed in SLOS cells. Identical changes in Kv1.3 operation were observed when control/healthy T cells were loaded with 7DHC. Removal of the putative sterol binding sites on Kv1.3 resulted in a phenotype that was not influenced by the elevation in membrane sterol level. Functional assays exhibited impaired activation and proliferation rate of T cells probably partially due to the modified Kv1.3 operation. We concluded that the altered membrane sterol composition hindered the operation of Kv1.3 as well as the ion channel-controlled T cell functions. © 2016, Springer-Verlag Berlin Heidelberg. LA - English DB - MTMT ER - TY - JOUR AU - Kovács, Tamás AU - Batta, Gyula Gábor (Ifj.) AU - Hajdu, Tímea AU - Szabó, Ágnes Tímea AU - Váradi, Tímea Erzsébet AU - Zákány, Florina AU - Csomós, István AU - Szöllősi, János AU - Nagy, Péter TI - The dipole potential modifies the clustering and ligand binding affinity of ErbB proteins and their signaling efficiency JF - SCIENTIFIC REPORTS J2 - SCI REP VL - 6 PY - 2016 PG - 11 SN - 2045-2322 DO - 10.1038/srep35850 UR - https://m2.mtmt.hu/api/publication/3130199 ID - 3130199 N1 - Funding Agency and Grant Number: National Research, Development and Innovation Office, Hungary [K103906, NK101337, GINOP-2.3.2-15-2016-00020]; European Social FundEuropean Social Fund (ESF) [TAMOP-4.2.2.A-11/1/KONV-2012-0025]; European UnionEuropean Commission Funding text: This work has been supported by research grants from the National Research, Development and Innovation Office, Hungary (K103906, NK101337, GINOP-2.3.2-15-2016-00020) and by the European Union and the European Social Fund (TAMOP-4.2.2.A-11/1/KONV-2012-0025). LA - English DB - MTMT ER - TY - JOUR AU - Izsépi, Emese AU - Himer, L AU - Szilágyi, Orsolya AU - Hajdu, Péter Béla AU - Panyi, György AU - László, Glória AU - Matkó, János TI - Membrane microdomain patterns, calcium signaling and NFAT activation as an important axis in determining polarized Th cell function JF - CYTOMETRY PART A J2 - CYTOM PART A VL - 83 PY - 2013 IS - 2 SP - 185 EP - 196 PG - 12 SN - 1552-4922 DO - 10.1002/cyto.a.22234 UR - https://m2.mtmt.hu/api/publication/2017137 ID - 2017137 LA - English DB - MTMT ER - TY - JOUR AU - Somodi, Sándor AU - Balajthy, András AU - Szilágyi, Orsolya AU - Pethő, Zoltán Dénes AU - Harangi, Mariann AU - Paragh, György AU - Panyi, György AU - Hajdu, Péter Béla TI - Analysis of the K+ current in human CD4+ T lymphocytes in hypercholesterolemic state JF - CELLULAR IMMUNOLOGY J2 - CELL IMMUNOL VL - 281 PY - 2013 IS - 1 SP - 20 EP - 26 PG - 7 SN - 0008-8749 DO - 10.1016/j.cellimm.2013.01.004 UR - https://m2.mtmt.hu/api/publication/2239256 ID - 2239256 N1 - 162058 AB - Atherosclerosis involves immune mechanisms: T lymphocytes are found in atherosclerotic plaques, suggesting their activation during atherogenesis. The predominant voltage-gated potassium channel of T cells, Kv1.3 is a key regulator of the Ca2+-dependent activation pathway. In the present experiments we studied the proliferation capacity and functional changes of Kv1.3 channels in T cells from healthy and hypercholestaeremic patients.By means of CFSE-assay (carboxyfluorescein succinimidyl ester) we showed that spontaneous activation rate of lymphocytes in hypercholesterolemia was elevated and the antiCD3/antiCD28 co-stimulation was less effective as compared to the healthy group. Using whole-cell patch-clamping we obtained that the activation and deactivation kinetics of Kv1.3 channels were faster in hypercholesterolemic state but no change in other parameters of Kv1.3 were found (inactivation kinetics, steady-state activation, expression level). We suppose that incorporation of oxLDL species via its raft-rupturing effect can modify proliferative rate of T cells as well as the gating of Kv1.3 channels. © 2013 Elsevier Inc. LA - English DB - MTMT ER - TY - JOUR AU - Panyi, György AU - Vámosi, György AU - Bacsó, Zsolt AU - Bagdány, M AU - Bodnár, Andrea AU - Varga, Zoltán AU - Gáspár, Rezső AU - Mátyus, László AU - Damjanovich, Sándor TI - Kv1.3 potassium channels are localized in the immunological synapse formed between cytotoxic and target cells JF - PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA J2 - P NATL ACAD SCI USA VL - 101 PY - 2004 IS - 5 SP - 1285 EP - 1290 PG - 6 SN - 0027-8424 DO - 10.1073/pnas.0307421100 UR - https://m2.mtmt.hu/api/publication/1075641 ID - 1075641 N1 - Panyi G, Vamosi G, Bacso Z are equal first authors LA - English DB - MTMT ER - TY - JOUR AU - Vámosi, György AU - Bacso, Z AU - Bodnár, Andrea AU - Bagdany, M AU - Varga, Z AU - Gaspar, R AU - Mátyus, László AU - Damjanovich, Sándor AU - Panyi, György TI - Kv1.3 potassium channels are localized in the immunological synapse formed between cytotoxic and target cells JF - BIOPHYSICAL JOURNAL J2 - BIOPHYS J VL - 86 PY - 2004 IS - 1 SP - 540A EP - 540A SN - 0006-3495 UR - https://m2.mtmt.hu/api/publication/3335428 ID - 3335428 LA - English DB - MTMT ER - TY - JOUR AU - Hajdu, Péter Béla AU - Varga, Zoltán AU - Pieri, C AU - Panyi, György AU - Gáspár, Rezső TI - Cholesterol modifies the gating of Kv1.3 in human T lymphocytes JF - PFLUGERS ARCHIV-EUROPEAN JOURNAL OF PHYSIOLOGY J2 - PFLUG ARCH EUR J PHY VL - 445 PY - 2003 IS - 6 SP - 674 EP - 682 PG - 9 SN - 0031-6768 DO - 10.1007/s00424-002-0974-y UR - https://m2.mtmt.hu/api/publication/105647 ID - 105647 LA - English DB - MTMT ER -