{ "labelLang" : "hun", "responseDate" : "2024-03-28 13:11", "paging" : { "last" : true, "first" : true, "totalPages" : 1, "totalElements" : 18, "totalUncutElements" : 18, "totalEstimatedElements" : 1, "size" : 100, "number" : 1, "numberOfElements" : 18, "sort" : [ { "direction" : "DESC", "property" : "publishedYear", "ascending" : false }, { "direction" : "ASC", "property" : "firstAuthor", "ascending" : true }, { "direction" : "ASC", "property" : "title", "ascending" : true } ] }, "content" : [ { "otype" : "JournalArticle", "mtid" : 2593568, "status" : "ADMIN_APPROVED", "published" : true, "unhandledTickets" : 0, "oldTimestamp" : "2014-12-24T11:31:11.000+0000", "deleted" : false, "oldId" : 2593568, "lastRefresh" : "2024-02-27T17:42:00.591+0000", "lastModified" : "2020-12-05T13:15:08.149+0000", "created" : "2014-05-27T07:24:18.000+0000", "creator" : { "otype" : "Author", "mtid" : 10008996, "link" : "/api/author/10008996", "label" : "Deli Mária Anna (Neurobiológia, vér-agy gát)", "familyName" : "Deli", "givenName" : "Mária Anna", "published" : true, "oldId" : 10008996, "snippet" : true }, "lastDuplumOK" : "2024-03-01T09:47:54.014+0000", "lastDuplumSearch" : "2024-03-01T09:47:54.014+0000", "adminApproved" : "2019-03-18T08:29:49.897+0000", "adminApprover" : { "otype" : "Admin", "mtid" : 10018677, "link" : "/api/admin/10018677", "label" : "Berkes Kata (SZBK 5admin)", "familyName" : "Berkes", "givenName" : "Kata", "published" : true, "oldId" : 10018677, "snippet" : true }, "core" : true, "citation" : true, "publicationPending" : false, "type" : { "otype" : "PublicationType", "mtid" : 24, "link" : "/api/publicationtype/24", "label" : "Folyóiratcikk", "code" : 24, "otypeName" : "JournalArticle", "listPosition" : 1, "published" : true, "oldId" : 24, "snippet" : true }, "subType" : { "otype" : "SubType", "mtid" : 10000059, "link" : "/api/subtype/10000059", "label" : "Szakcikk (Folyóiratcikk)", "name" : "Szakcikk", "nameEng" : "Article", "docType" : { "otype" : "PublicationType", "mtid" : 24, "link" : "/api/publicationtype/24", "label" : "Folyóiratcikk", "code" : 24, "otypeName" : "JournalArticle", "listPosition" : 1, "published" : true, "oldId" : 24, "snippet" : true }, "listPosition" : 101, "published" : true, "oldId" : 10000059, "snippet" : true }, "category" : { "otype" : "Category", "mtid" : 1, "link" : "/api/category/1", "label" : "Tudományos", "published" : true, "oldId" : 1, "snippet" : true }, "languages" : [ { "otype" : "Language", "mtid" : 10002, "link" : "/api/language/10002", "label" : "Angol", "name" : "Angol", "nameEng" : "English", "published" : true, "oldId" : 2, "snippet" : true } ], "firstAuthor" : "Jähne, EA", "authorships" : [ { "otype" : "PersonAuthorship", "mtid" : 5144787, "link" : "/api/authorship/5144787", "label" : "Jähne, EA", "listPosition" : 1, "share" : 0.09090909, "first" : true, "last" : false, "familyName" : "Jähne", "givenName" : "EA", "authorTyped" : true, "editorTyped" : false, "otherTyped" : false, "type" : { "otype" : "AuthorshipType", "mtid" : 1, "link" : "/api/authorshiptype/1", "label" : "Szerző", "code" : 0, "published" : true, "oldId" : 0, "snippet" : true }, "published" : false, "oldId" : 19721717, "snippet" : true }, { "otype" : "PersonAuthorship", "mtid" : 5144788, "link" : "/api/authorship/5144788", "label" : "Eigenmann, DE", "listPosition" : 2, "share" : 0.09090909, "first" : false, "last" : false, "familyName" : "Eigenmann", "givenName" : "DE", "authorTyped" : true, "editorTyped" : false, "otherTyped" : false, "type" : { "otype" : "AuthorshipType", "mtid" : 1, "link" : "/api/authorshiptype/1", "label" : "Szerző", "code" : 0, "published" : true, "oldId" : 0, "snippet" : true }, "published" : false, "oldId" : 19721716, "snippet" : true }, { "otype" : "PersonAuthorship", "mtid" : 5144789, "link" : "/api/authorship/5144789", "label" : "Culot, M", "listPosition" : 3, "share" : 0.09090909, "first" : false, "last" : false, "familyName" : "Culot", "givenName" : "M", "authorTyped" : true, "editorTyped" : false, "otherTyped" : false, "type" : { "otype" : "AuthorshipType", "mtid" : 1, "link" : "/api/authorshiptype/1", "label" : "Szerző", "code" : 0, "published" : true, "oldId" : 0, "snippet" : true }, "published" : false, "oldId" : 19721715, "snippet" : true }, { "otype" : "PersonAuthorship", "mtid" : 5144790, "link" : "/api/authorship/5144790", "label" : "Cecchelli, R", "listPosition" : 4, "share" : 0.09090909, "first" : false, "last" : false, "familyName" : "Cecchelli", "givenName" : "R", "authorTyped" : true, "editorTyped" : false, "otherTyped" : false, "type" : { "otype" : "AuthorshipType", "mtid" : 1, "link" : "/api/authorshiptype/1", "label" : "Szerző", "code" : 0, "published" : true, "oldId" : 0, "snippet" : true }, "published" : false, "oldId" : 19721714, "snippet" : true }, { "otype" : "PersonAuthorship", "mtid" : 5144791, "link" : "/api/authorship/5144791", "label" : "Walter, FR [Walter, Fruzsina (biofizika), szerző] Biofizikai Intézet (HRN SZBK)", "listPosition" : 5, "share" : 0.09090909, "first" : false, "last" : false, "author" : { "otype" : "Author", "mtid" : 10032624, "link" : "/api/author/10032624", "label" : "Walter Fruzsina (biofizika)", "familyName" : "Walter", "givenName" : "Fruzsina", "published" : true, "oldId" : 10032624, "snippet" : true }, "familyName" : "Walter", "givenName" : "FR", "authorTyped" : true, "editorTyped" : false, "otherTyped" : false, "type" : { "otype" : "AuthorshipType", "mtid" : 1, "link" : "/api/authorshiptype/1", "label" : "Szerző", "code" : 0, "published" : true, "oldId" : 0, "snippet" : true }, "published" : false, "oldId" : 19721713, "snippet" : true }, { "otype" : "PersonAuthorship", "mtid" : 5144792, "link" : "/api/authorship/5144792", "label" : "Deli, MA [Deli, Mária Anna (Neurobiológia, vé...), szerző] Biofizikai Intézet (HRN SZBK)", "listPosition" : 6, "share" : 0.09090909, "first" : false, "last" : false, "author" : { "otype" : "Author", "mtid" : 10008996, "link" : "/api/author/10008996", "label" : "Deli Mária Anna (Neurobiológia, vér-agy gát)", "familyName" : "Deli", "givenName" : "Mária Anna", "published" : true, "oldId" : 10008996, "snippet" : true }, "familyName" : "Deli", "givenName" : "MA", "authorTyped" : true, "editorTyped" : false, "otherTyped" : false, "type" : { "otype" : "AuthorshipType", "mtid" : 1, "link" : "/api/authorshiptype/1", "label" : "Szerző", "code" : 0, "published" : true, "oldId" : 0, "snippet" : true }, "published" : false, "oldId" : 19721712, "snippet" : true }, { "otype" : "PersonAuthorship", "mtid" : 5144793, "link" : "/api/authorship/5144793", "label" : "Tremmel, R", "listPosition" : 7, "share" : 0.09090909, "first" : false, "last" : false, "familyName" : "Tremmel", "givenName" : "R", "authorTyped" : true, "editorTyped" : false, "otherTyped" : false, "type" : { "otype" : "AuthorshipType", "mtid" : 1, "link" : "/api/authorshiptype/1", "label" : "Szerző", "code" : 0, "published" : true, "oldId" : 0, "snippet" : true }, "published" : false, "oldId" : 19721711, "snippet" : true }, { "otype" : "PersonAuthorship", "mtid" : 5144794, "link" : "/api/authorship/5144794", "label" : "Fricker, G", "listPosition" : 8, "share" : 0.09090909, "first" : false, "last" : false, "familyName" : "Fricker", "givenName" : "G", "authorTyped" : true, "editorTyped" : false, "otherTyped" : false, "type" : { "otype" : "AuthorshipType", "mtid" : 1, "link" : "/api/authorshiptype/1", "label" : "Szerző", "code" : 0, "published" : true, "oldId" : 0, "snippet" : true }, "published" : false, "oldId" : 19721710, "snippet" : true }, { "otype" : "PersonAuthorship", "mtid" : 5144795, "link" : "/api/authorship/5144795", "label" : "Smiesko, M", "listPosition" : 9, "share" : 0.09090909, "first" : false, "last" : false, "familyName" : "Smiesko", "givenName" : "M", "authorTyped" : true, "editorTyped" : false, "otherTyped" : false, "type" : { "otype" : "AuthorshipType", "mtid" : 1, "link" : "/api/authorshiptype/1", "label" : "Szerző", "code" : 0, "published" : true, "oldId" : 0, "snippet" : true }, "published" : false, "oldId" : 19721709, "snippet" : true }, { "otype" : "PersonAuthorship", "mtid" : 5144796, "link" : "/api/authorship/5144796", "label" : "Hamburger, M", "listPosition" : 10, "share" : 0.09090909, "first" : false, "last" : false, "familyName" : "Hamburger", "givenName" : "M", "authorTyped" : true, "editorTyped" : false, "otherTyped" : false, "type" : { "otype" : "AuthorshipType", "mtid" : 1, "link" : "/api/authorshiptype/1", "label" : "Szerző", "code" : 0, "published" : true, "oldId" : 0, "snippet" : true }, "published" : false, "oldId" : 19721708, "snippet" : true }, { "otype" : "PersonAuthorship", "mtid" : 5144797, "link" : "/api/authorship/5144797", "label" : "Oufir, M", "listPosition" : 11, "share" : 0.09090909, "first" : false, "last" : true, "familyName" : "Oufir", "givenName" : "M", "authorTyped" : true, "editorTyped" : false, "otherTyped" : false, "type" : { "otype" : "AuthorshipType", "mtid" : 1, "link" : "/api/authorshiptype/1", "label" : "Szerző", "code" : 0, "published" : true, "oldId" : 0, "snippet" : true }, "published" : false, "oldId" : 19721707, "snippet" : true } ], "title" : "Development and validation of a LC-MS/MS method for assessment of an anti-inflammatory indolinone derivative by in vitro blood-brain barrier models", "identifiers" : [ { "otype" : "PublicationIdentifier", "mtid" : 806996, "link" : "/api/publicationidentifier/806996", "label" : "DOI: 10.1016/j.jpba.2014.05.026", "source" : { "otype" : "PlainSource", "mtid" : 6, "link" : "/api/publicationsource/6", "label" : "DOI", "type" : { "otype" : "PublicationSourceType", "mtid" : 10001, "link" : "/api/publicationsourcetype/10001", "label" : "DOI", "mayHaveOa" : true, "published" : true, "snippet" : true }, "name" : "DOI", "nameEng" : "DOI", "linkPattern" : "https://doi.org/@@@", "publiclyVisible" : true, "published" : true, "oldId" : 6, "snippet" : true }, "oaType" : "GOLD", "oaFree" : true, "validState" : "IDENTICAL", "idValue" : "10.1016/j.jpba.2014.05.026", "realUrl" : "https://doi.org/10.1016/j.jpba.2014.05.026", "published" : false, "oldId" : 695436, "snippet" : true }, { "otype" : "PublicationIdentifier", "mtid" : 14850572, "link" : "/api/publicationidentifier/14850572", "label" : "ScienceDirect: S0731708514002660", "source" : { "otype" : "PlainSource", "mtid" : 27, "link" : "/api/publicationsource/27", "label" : "ScienceDirect", "type" : { "otype" : "PublicationSourceType", "mtid" : 10004, "link" : "/api/publicationsourcetype/10004", "label" : "Kiadói adatbázis", "mayHaveOa" : false, "published" : true, "snippet" : true }, "name" : "ScienceDirect", "linkPattern" : "http://www.sciencedirect.com/science/article/pii/@@@", "publiclyVisible" : true, "published" : true, "oldId" : 27, "snippet" : true }, "validState" : "NO", "idValue" : "S0731708514002660", "realUrl" : "http://www.sciencedirect.com/science/article/pii/S0731708514002660", "published" : false, "oldId" : 6916508, "snippet" : true }, { "otype" : "PublicationIdentifier", "mtid" : 806995, "link" : "/api/publicationidentifier/806995", "label" : "WoS: 000339859800031", "source" : { "otype" : "PlainSource", "mtid" : 1, "link" : "/api/publicationsource/1", "label" : "WoS", "type" : { "otype" : "PublicationSourceType", "mtid" : 10003, "link" : "/api/publicationsourcetype/10003", "label" : "Indexelő adatbázis", "mayHaveOa" : false, "published" : true, "snippet" : true }, "name" : "WoS", "nameEng" : "WoS", "linkPattern" : "https://www.webofscience.com/wos/woscc/full-record/@@@", "publiclyVisible" : true, "published" : true, "oldId" : 1, "snippet" : true }, "oaFree" : false, "validState" : "IDENTICAL", "idValue" : "000339859800031", "realUrl" : "https://www.webofscience.com/wos/woscc/full-record/000339859800031", "published" : false, "oldId" : 1071544, "snippet" : true }, { "otype" : "PublicationIdentifier", "mtid" : 14850574, "link" : "/api/publicationidentifier/14850574", "label" : "Scopus: 84902478877", "source" : { "otype" : "PlainSource", "mtid" : 3, "link" : "/api/publicationsource/3", "label" : "Scopus", "type" : { "otype" : "PublicationSourceType", "mtid" : 10003, "link" : "/api/publicationsourcetype/10003", "label" : "Indexelő adatbázis", "mayHaveOa" : false, "published" : true, "snippet" : true }, "name" : "Scopus", "linkPattern" : "http://www.scopus.com/record/display.url?origin=inward&eid=2-s2.0-@@@", "publiclyVisible" : true, "published" : true, "oldId" : 3, "snippet" : true }, "validState" : "IDENTICAL", "idValue" : "84902478877", "realUrl" : "http://www.scopus.com/record/display.url?origin=inward&eid=2-s2.0-84902478877", "published" : false, "oldId" : 17407488, "snippet" : true }, { "otype" : "PublicationIdentifier", "mtid" : 806997, "link" : "/api/publicationidentifier/806997", "label" : "PubMed: 24949819", "source" : { "otype" : "PlainSource", "mtid" : 17, "link" : "/api/publicationsource/17", "label" : "PubMed", "type" : { "otype" : "PublicationSourceType", "mtid" : 10003, "link" : "/api/publicationsourcetype/10003", "label" : "Indexelő adatbázis", "mayHaveOa" : false, "published" : true, "snippet" : true }, "name" : "PubMed", "nameEng" : "PubMed", "linkPattern" : "http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&list_uids=@@@&dopt=Abstract", "publiclyVisible" : true, "published" : true, "oldId" : 17, "snippet" : true }, "oaFree" : false, "validState" : "NO", "idValue" : "24949819", "realUrl" : "http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&list_uids=24949819&dopt=Abstract", "published" : false, "oldId" : 913236, "snippet" : true }, { "otype" : "PublicationIdentifier", "mtid" : 14850577, "link" : "/api/publicationidentifier/14850577", "label" : "Teljes dokumentum: http://www.sciencedirect.com/science/article/pii/S0731708514002660", "source" : { "otype" : "PlainSource", "mtid" : 39, "link" : "/api/publicationsource/39", "label" : "Teljes dokumentum", "type" : { "otype" : "PublicationSourceType", "mtid" : 10006, "link" : "/api/publicationsourcetype/10006", "label" : "Link", "mayHaveOa" : true, "published" : true, "snippet" : true }, "name" : "Teljes dokumentum", "nameEng" : "Teljes dokumentum", "linkPattern" : "@@@", "publiclyVisible" : true, "published" : true, "oldId" : 39, "snippet" : true }, "validState" : "NO", "idValue" : "http://www.sciencedirect.com/science/article/pii/S0731708514002660", "realUrl" : "http://www.sciencedirect.com/science/article/pii/S0731708514002660", "published" : false, "oldId" : 6916509, "snippet" : true } ], "journal" : { "otype" : "Journal", "mtid" : 2766, "link" : "/api/journal/2766", "label" : "JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS 0731-7085 1873-264X", "pIssn" : "0731-7085", "eIssn" : "1873-264X", "reviewType" : "REVIEWED", "noIF" : false, "sciIndexed" : true, "scopusIndexed" : true, "lang" : "FOREIGN", "hungarian" : false, "published" : true, "oldId" : 2766, "snippet" : true }, "volume" : "98", "firstPage" : "235", "lastPage" : "246", "firstPageOrInternalIdForSort" : "235", "pageLength" : 12, "publishedYear" : 2014, "abstractText" : "The compound (E,Z)-3-(4-hydroxy-3,5-dimethoxybenzylidene)indolin-2-one (indolinone) was identified from lipophilic woad extracts (Isatis tinctoria L., Brassicaceae) as a compound possessing potent histamine release inhibitory and anti-inflammatory properties [1]. To further evaluate the potential of indolinone in terms of crossing the blood–brain barrier (BBB), we screened the compound in several in vitro cell-based human and animal BBB models. Therefore, we developed a quantitative LC–MS/MS method for the compound in modified Ringer HEPES buffer (RHB) and validated it according to FDA and EMA guidelines [2] and [3]. The calibration curve of indolinone in the range between 30.0 and 3000 ng/ml was quadratic, and the limit of quantification was 30.0 ng/ml. Dilution of samples up to 100-fold did not affect precision and accuracy. The carry-over was within acceptance criteria. Indolinone proved to be stable in RHB for 3 h at room temperature (RT), and for three successive freeze/thaw cycles. The processed samples could be stored in the autosampler at 10 °C for at least 28 h. Moreover, indolinone was stable for at least 16 days in RHB when stored below −65 °C. This validation study demonstrates that our method is specific, selective, precise, accurate, and capable to produce reliable results.\n\nIn the immortalized human BBB mono-culture model, the apparent permeability coefficient from apical to basolateral (PappA→B), and the Papp from basolateral to apical (PappB→A) were 19.2 ± 0.485 × 10−6 cm/s and 21.7 ± 0.326 × 10−6 cm/s, respectively. For the primary rat/bovine BBB co-culture model a PappA→B of 27.1 ± 1.67 × 10−6 cm/s was determined. In the primary rat BBB triple co-culture model, the PappA→B and the PappB→A were 56.2 ± 3.63 × 10−6 cm/s and 34.6 ± 1.41 × 10−6 cm/s, respectively. The data obtained with the different models showed good correlation and were indicative of a high BBB permeation potential of indolinone confirmed by in silico prediction calculations. P-glycoprotein (P-gp) interaction for indolinone was studied with the aid of a calcein-AM uptake assay, and by calculation of the efflux ratio (ER) from the bidirectional permeability assays. For both bidirectional BBB models an ER below 2 was calculated, indicating that no active mediated transport mechanism is involved for indolinone. In porcine brain capillary endothelial cells (PBCECs), the calcein-AM uptake assay demonstrated that indolinone is neither a P-gp substrate nor a P-gp inhibitor and is accumulated into cells at high extent.", "subjects" : [ { "otype" : "Classification", "mtid" : 12125, "link" : "/api/classification/12125", "label" : "Általános orvostudomány", "published" : true, "snippet" : true }, { "otype" : "Classification", "mtid" : 10293, "link" : "/api/classification/10293", "label" : "Kémiai tudományok", "published" : true, "snippet" : true }, { "otype" : "Classification", "mtid" : 12215, "link" : "/api/classification/12215", "label" : "Klinikai orvostan", "published" : true, "snippet" : true } ], "digital" : null, "printed" : null, "sourceYear" : 2014, "foreignEdition" : true, "foreignLanguage" : true, "fullPublication" : true, "conferencePublication" : false, "nationalOrigin" : true, "missingAuthor" : false, "oaType" : "GOLD", "oaCheckDate" : "2024-02-27", "oaFree" : true, "oaLink" : "https://doi.org/10.1016/j.jpba.2014.05.026", "citationCount" : 10, "citationCountUnpublished" : 0, "citationCountWoOther" : 9, "independentCitCountWoOther" : 2, "nationalOriginCitationCount" : 4, "foreignEditionCitationCount" : 9, "doiCitationCount" : 9, "wosCitationCount" : 9, "scopusCitationCount" : 8, "wosScopusCitationCount" : 9, "wosScopusCitationCountWoOther" : 9, "wosScopusIndependentCitationCount" : 2, "wosScopusIndependentCitationCountWoOther" : 2, "independentCitationCount" : 3, "selfCitationCount" : 7, "unhandledCitationCount" : 0, "citingPubCount" : 10, "independentCitingPubCount" : 3, "citingPubCountWoOther" : 9, "independentCitingPubCountWoOther" : 2, "unhandledCitingPubCount" : 0, "citedPubCount" : 6, "citedCount" : 6, "ratings" : [ { "otype" : "SjrRating", "mtid" : 11100773, "link" : "/api/sjrrating/11100773", "label" : "sjr:D1 (2014) Scopus - Pharmaceutical Science JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS 0731-7085 1873-264X", "listPos" : 21, "rankValue" : 0.1, "type" : "journal", "ratingType" : { "otype" : "RatingType", "mtid" : 10002, "link" : "/api/ratingtype/10002", "label" : "sjr", "code" : "sjr", "published" : true, "snippet" : true }, "subject" : { "otype" : "ClassificationExternal", "mtid" : 3003, "link" : "/api/classificationexternal/3003", "label" : "Scopus - Pharmaceutical Science", "published" : true, "oldId" : 3003, "snippet" : true }, "ranking" : "D1", "calculation" : "DIRECT", "published" : true, "snippet" : true } ], "ratingsForSort" : "D1", "hasCitationDuplums" : false, "userChangeableUntil" : "2014-07-25T07:32:13.000+0000", "publishDate" : "2014-05-27T07:24:46.000+0000", "directInstitutesForSort" : "Biofizikai Intézet (HRN SZBK)", "ownerAuthorCount" : 2, "ownerInstituteCount" : 12, "directInstituteCount" : 1, "authorCount" : 11, "contributorCount" : 0, "hasQualityFactor" : true, "link" : "/api/publication/2593568", "label" : "Jähne EA et al. Development and validation of a LC-MS/MS method for assessment of an anti-inflammatory indolinone derivative by in vitro blood-brain barrier models. (2014) JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS 0731-7085 1873-264X 98 235-246", "template" : "