@article{MTMT:1536705, title = {Phase advancement and nucleus-specific timing of thalamocortical activity during slow cortical oscillation}, url = {https://m2.mtmt.hu/api/publication/1536705}, author = {Slézia, Andrea and Hangya, Balázs and Ulbert, István and Acsády, László}, doi = {10.1523/JNEUROSCI.3375-10.2011}, journal-iso = {J NEUROSCI}, journal = {JOURNAL OF NEUROSCIENCE}, volume = {31}, unique-id = {1536705}, issn = {0270-6474}, abstract = {The exact timing of cortical afferent activity is instrumental for the correct coding and retrieval of internal and external stimuli. Thalamocortical inputs represent the most significant subcortical pathway to the cortex, but the precise timing and temporal variability of thalamocortical activity is not known. To examine this question, we studied the phase of thalamic action potentials relative to cortical oscillations and established correlations among phase, the nuclear location of the thalamocortical neurons, and the frequency of cortical activity. The phase of thalamic action potentials depended on the exact frequency of the slow cortical oscillation both on long (minutes) and short (single wave) time scales. Faster waves were accompanied by phase advancement in both cases. Thalamocortical neurons located in different nuclei fired at significantly different phases of the slow waves but were active at a similar phase of spindle oscillations. Different thalamic nuclei displayed distinct burst patterns. Bursts with a higher number of action potentials displayed progressive phase advancement in a nucleus-specific manner. Thalamic neurons located along nuclear borders were characterized by mixed burst and phase properties. Our data demonstrate that the temporal relationship between cortical and thalamic activity is not fixed but displays dynamic changes during oscillatory activity. The timing depends on the precise location and exact activity of thalamocortical cells and the ongoing cortical network pattern. This variability of thalamic output and its coupling to cortical activity can enable thalamocortical neurons to actively participate in the coding and retrieval of cortical signals.}, keywords = {Animals; Male; RATS; Periodicity; Action Potentials; Rats, Wistar; Cerebral Cortex/*physiology; Neurons/physiology; Thalamus/*physiology}, year = {2011}, eissn = {1529-2401}, pages = {607-617}, orcid-numbers = {Slézia, Andrea/0000-0002-4528-3169; Ulbert, István/0000-0001-9941-9159} } @article{MTMT:109948, title = {Contrasting the functional properties of GABAergic axon terminals with single and multiple synapses in the thalamus}, url = {https://m2.mtmt.hu/api/publication/109948}, author = {Wanaverbecq, N and Bodor, Ágnes and Bokor, Hajnalka and Slézia, Andrea and Luthi, A and Acsády, László}, doi = {10.1523/JNEUROSCI.3183-08.2008}, journal-iso = {J NEUROSCI}, journal = {JOURNAL OF NEUROSCIENCE}, volume = {28}, unique-id = {109948}, issn = {0270-6474}, abstract = {Diverse sources of GABAergic inhibition are a major feature of cortical networks, but distinct inhibitory input systems have not been systematically characterized in the thalamus. Here, we contrasted the properties of two independent GABAergic pathways in the posterior thalamic nucleus of rat, one input from the reticular thalamic nucleus (nRT), and one "extrareticular" input from the anterior pretectal nucleus (APT). The vast majority of nRT-thalamic terminals formed single synapses per postsynaptic target and innervated thin distal dendrites of relay cells. In contrast, single APT-thalamic terminals formed synaptic contacts exclusively via multiple, closely spaced synapses on thick relay cell dendrites. Quantal analysis demonstrated that the two inputs displayed comparable quantal amplitudes, release probabilities, and multiple release sites. The morphological and physiological data together indicated multiple, single-site contacts for nRT and multisite contacts for APT axons. The contrasting synaptic arrangements of the two pathways were paralleled by different short-term plasticities. The multisite APT-thalamic pathway showed larger charge transfer during 50-100 Hz stimulation compared with the nRT pathway and a greater persistent inhibition accruing during stimulation trains. Our results demonstrate that the two inhibitory systems are morpho-functionally distinct and suggest and that multisite GABAergic terminals are tailored for maintained synaptic inhibition even at high presynaptic firing rates. These data explain the efficacy of extrareticular inhibition in timing relay cell activity in sensory and motor thalamic nuclei. Finally, based on the classic nomenclature and the difference between reticular and extrareticular terminals, we define a novel, multisite GABAergic terminal type (F3) in the thalamus.}, year = {2008}, eissn = {1529-2401}, pages = {11848-11861}, orcid-numbers = {Slézia, Andrea/0000-0002-4528-3169} } @article{MTMT:1204243, title = {Postsynaptic positioning of endocytic zones and AMPA receptor cycling by physical coupling of dynamin-3 to homer}, url = {https://m2.mtmt.hu/api/publication/1204243}, author = {Lu, J and Helton, TD and Blanpied, TA and Rácz, Bence and Newpher, TM and Weinberg, RJ and Ehlers, MD}, doi = {10.1016/j.neuron.2007.06.041}, journal-iso = {NEURON}, journal = {NEURON}, volume = {55}, unique-id = {1204243}, issn = {0896-6273}, abstract = {Endocytosis; of AMPA receptors and other postsynaptic cargo occurs at endocytic zones (EZs), stably positioned sites of clathrin adjacent to the postsynaptic density (PSD). The tight localization of postsynaptic endocytosis is thought to control spine composition and regulate synaptic transmission. However, the mechanisms that situate the EZ near the PSD and the role of spine endocytosis in synaptic transmission are unknown. Here, we report that a physical link between dynamin-3 and the postsynaptic adaptor Homer positions the EZ near the PSD. Disruption of dynamin-3 or its interaction with Homer uncouples the PSD from the EZ, resulting in synapses lacking postsynaptic clathrin. Loss of the EZ leads to a loss of synaptic AMPA receptors and reduced excitatory synaptic transmission that corresponds with impaired synaptic recycling. Thus, a physical link between the PSD and the EZ ensures localized endocytosis and recycling by recapturing and maintaining a proximate pool of cycling AMPA receptors.}, keywords = {NMDA RECEPTORS; SYNAPTIC-TRANSMISSION; SYNAPSES; PLASTICITY; TRAFFICKING; ACTIN DYNAMICS; DENSITY PROTEINS; RECYCLING ENDOSOMES; GLUTAMATE RECEPTORS; DENDRITIC SPINES}, year = {2007}, eissn = {1097-4199}, pages = {874-889} } @article{MTMT:109610, title = {Selective GABAergic control of higher-order thalamic relays}, url = {https://m2.mtmt.hu/api/publication/109610}, author = {Bokor, Hajnalka and Frére, SG and Eyre, Mark David and Slézia, Andrea and Ulbert, István and Lüthi, A and Acsády, László}, doi = {10.1016/j.neuron.2005.01.048}, journal-iso = {NEURON}, journal = {NEURON}, volume = {45}, unique-id = {109610}, issn = {0896-6273}, year = {2005}, eissn = {1097-4199}, pages = {929-940}, orcid-numbers = {Slézia, Andrea/0000-0002-4528-3169; Ulbert, István/0000-0001-9941-9159} } @article{MTMT:1865302, title = {Atonia-related regions in the rodent pons and medulla}, url = {https://m2.mtmt.hu/api/publication/1865302}, author = {Hajnik, Tünde and Lai, YY and Siegel, JM}, journal-iso = {J NEUROPHYSIOL}, journal = {JOURNAL OF NEUROPHYSIOLOGY}, volume = {84}, unique-id = {1865302}, issn = {0022-3077}, abstract = {Electrical stimulation of circumscribed areas of the pontine and medullary reticular formation inhibits muscle tone in cats. In this report, we present an analysis of the anatomical distribution of atonia-inducing stimulation sites in the brain stem of the rat. Muscle atonia could be elicited by electrical stimulation of the nuclei reticularis pontis oralis and caudalis in the pons as well as the nuclei gigantocellularis, gigantocellularis alpha, gigantocellularis ventralis, and paragigantocellularis dorsalis in the medulla of decerebrate rats. This inhibitory effect on muscle tone was a function of the intensity and frequency of the electrical stimulation. Average latencies of muscle-tone suppressions elicited by electrical stimulation of the pontine reticular formation were 11.02 +/- 2.54 and 20.49 +/- 3.39 (SD) ms in the neck and in the hindlimb muscles, respectively. Following medullary stimulation, these latencies were 11.29 +/- 2.44 ms in the neck and 18.87 +/- 2.64 ms in the hindlimb muscles. Microinjection of N-methyl-D-aspartate (NMDA, 7 mM/0.1 ml) agonists into the pontine and medullary inhibitory sites produced muscle-tone facilitation, whereas quisqualate (10 mM/ 0.1 ml) injection induced an inhibition of muscle tone. NMDA-induced muscle tone change had a latency of 31.8 +/- 35.3 s from the pons and 10.5 +/- 0.7 s from the medulla and a duration of 146.7 +/- 95.2 s from the pons and 55.5 +/- 40.4 s from the medulla. The latency of quisqualate (QU)-induced reduction of neck muscle tone was 30.1 +/- 37.9 s after pontine and 39.5 +/- 21.8 s after medullary injection. The duration of muscle-tone suppression induced by QU injection into the pons and medulla was 111.5 +/- 119.2 and 169.2 +/- 145.3 s. Smaller rats (8 wk old) had a higher percentage of sites producing muscle-tone inhibition than larger rats (16 wk old), indicating an age-related change in the function of brain stem inhibitory systems. The anatomical distribution of atonia-related sites in the rat has both similarities and differences with the distribution found in the cat, which can be explained by the distinct anatomical organization of the brain stem in these two species.}, year = {2000}, eissn = {1522-1598}, pages = {1942-1948}, orcid-numbers = {Hajnik, Tünde/0000-0003-0684-8136} } @article{MTMT:2941590, title = {LOW-FREQUENCY OSCILLATORY ACTIVITIES INTRINSIC TO RAT AND CAT THALAMOCORTICAL CELLS}, url = {https://m2.mtmt.hu/api/publication/2941590}, author = {LERESCHE, N and LIGHTOWLER, S and Soltesz, Ivan and JASSIKGERSCHENFELD, D and CRUNELLI, V}, journal-iso = {J PHYSIOL-LONDON}, journal = {JOURNAL OF PHYSIOLOGY-LONDON}, volume = {441}, unique-id = {2941590}, issn = {0022-3751}, abstract = {1. Low-frequency membrane potential oscillations recorded intracellularly from thalamocortical (TC) cells of the rat and cat dorsal lateral geniculate nucleus (dLGN) and of the rat ventrobasal nucleus (VB) maintained in vitro were investigated. On the basis of their electrophysiological and pharmacological properties, four types of activity were distinguished and named: the pacemaker oscillations, the spindle-like oscillations, the 'very slow' oscillations and the 'N-methyl-D-aspartate' (NMDA) oscillations. 2. The pacemaker oscillations (95 out of 173 cells) consisted of rhythmic, large-amplitude (10-30 mV) depolarizations which occurred at a frequency of 1.8 +/- 0.3 Hz (range, 0.5-2.9 Hz) and could often give rise to single or a burst of action potentials. Pacemaker oscillations were observed when the membrane potential was moved negative to -55 and positive to -80 mV, but in a given cell the upper and lower limits of this voltage range were separated by only 13.1 +/- 0.5 mV. Above -45 mV tonic firing consisting of single action potentials was seen in the cells showing this or the other types of low-frequency oscillations. 3. The spindle-like oscillations were observed in thirty-nine (out of 173) TC cells and consisted of rhythmic (2.1 +/- 0.3 Hz), large-amplitude depolarizations (and often associated burst firing) similar to the pacemaker oscillations but occurring in discrete periods every 5-25 s and lasting for 1.5-28 s. The spindle-like oscillations were observed when the membrane potential was moved negative to -55 and positive to -80 mV and in two cells they were transformed into continuous pacemaker oscillations by depolarization of the membrane potential to -60 mV. 4. Pacemaker and spindle-like oscillations were unaffected by tetrodotoxin (TTX) or by selective blockade of NMDA, non-NMDA, GABA(A), GABA(B), nicotinic, muscarinic, alpha- and beta-noradrenergic receptors. 5. The 'very slow' oscillations consisted of a TTX-insensitive, slow hyperpolarization-depolarization sequence (5-15 mV in amplitude) which lasted up to 90 s and was observed in nine dLGN cells and in two VB cells. The pacemaker and the spindle-like oscillations were recorded in one cell each which also showed the 'very slow' oscillations. 6. The 'NMDA' oscillations were observed only in a 'Mg2+-free' medium (0 mM-Mg2+, 2-4 mM-Ca2+; 64 out of 72 cells) and consisted of large-amplitude (10-25 mV) depolarizations that did not occur at regular intervals and were intermixed with smaller depolarizations present on the baseline and on the failing phase of the larger ones. The 'NMDA' oscillations were voltage dependent (observed in the range from -60 to -85 mV), insensitive to TTX and had a frequency of 1-4 Hz. Application of selective NMDA receptor antagonists reversibly transformed the NMDA into the pacemaker or the spindle-like oscillations while blockade of non-NMDA receptors as well as the other receptors mentioned above (paragraph 4) had no effect on the 'NMDA' oscillations. 7. The pacemaker, the spindle-like and the 'very slow' oscillations were never observed in electrophysiologically identified rat and cat dLGN interneurones (n = 12). Similarly, in a 'Mg2+-free' medium, interneurones in the dLGN and cells in the ventral lateral geniculate nucleus did not show the 'NMDA' oscillations but only slow, small-amplitude (< 4 mV) depolarizations that were reversibly abolished by DL-2-amino-5-phosphono-valeric acid (25-mu-M). 8. These results indicate that (i) single TC cells in different thalamic nuclei of different species are capable of four types of low-frequency oscillatory activity that do not require the evoked rhythmic recruitment of other neurones and (ii) activation of the NMDA receptors by spontaneously released excitatory amino acid brings about the transformation of the pacemaker and the spindle-like oscillations into the 'NMDA' oscillations.}, keywords = {GUINEA-PIG; LATERAL GENICULATE-NUCLEUS; NMDA RECEPTORS; CENTRAL NERVOUS-SYSTEM; ELECTROPHYSIOLOGICAL PROPERTIES; RELAY NEURONS; POSTSYNAPTIC POTENTIALS; BURST DISCHARGES; PARKINSONIAN TREMOR; PIG THALAMIC NEURONS}, year = {1991}, eissn = {1469-7793}, pages = {155-174} } @article{MTMT:103037, title = {Antisera to γ-aminobutyric acid : II. immunocytochemical application to the central nervous system}, url = {https://m2.mtmt.hu/api/publication/103037}, author = {Somogyi, Péter Pál and Hodgson, Anthony J and Chubb, Ian W and Penke, Botond and Erdei, Anna}, doi = {10.1177/33.3.2579123}, journal-iso = {J HISTOCHEM CYTOCHEM}, journal = {JOURNAL OF HISTOCHEMISTRY & CYTOCHEMISTRY}, volume = {33}, unique-id = {103037}, issn = {0022-1554}, year = {1985}, eissn = {1551-5044}, pages = {240-248}, orcid-numbers = {Penke, Botond/0000-0003-0938-0567; Erdei, Anna/0000-0002-3622-6680} }