@article{MTMT:2390640,
	title = {Brain protein expression changes in WAG/Rij rats, a genetic rat model of absence epilepsy after peripheral lipopolysaccharide treatment},
	url = {https://m2.mtmt.hu/api/publication/2390640},
	author = {Györffy, Balázs and Kovács, Zsolt and Gulyássy, Péter and Simor, Attila and Völgyi, Katalin and Orbán, Gergely and Baracskay, Péter and Szabó, Zoltán and Janáky, Tamás and Dobolyi, Árpád and Juhász, Gábor Dénes and Czurkó, András and Kékesi, Adrienna Katalin},
	doi = {10.1016/j.bbi.2013.09.001},
	journal-iso = {BRAIN BEHAV IMMUN},
	journal = {BRAIN BEHAVIOR AND IMMUNITY},
	volume = {35},
	unique-id = {2390640},
	issn = {0889-1591},
	abstract = {Peripheral injection of bacterial lipopolysaccharide (LPS) facilitates 8–10 Hz spike-wave discharges (SWD) characterizing absence epilepsy in WAG/Rij rats. It is unknown however, whether peripherally administered LPS is able to alter the generator areas of epileptic activity at the molecular level. We injected 1 mg/kg dose of LPS intraperitoneally into WAG/Rij rats, recorded the body temperature and EEG, and examined the protein expression changes of the proteome 12 h after injection in the fronto-parietal cortex and thalamus. We used fluorescent two-dimensional differential gel electrophoresis to investigate the expression profile. We found 16 differentially expressed proteins in the fronto-parietal cortex and 35 proteins in the thalamus. It is known that SWD genesis correlates with the transitional state of sleep–wake cycle thus we performed meta-analysis of the altered proteins in relation to inflammation, epilepsy as well as sleep. The analysis revealed that all categories are highly represented by the altered proteins and these protein-sets have considerable overlap. Protein network modeling suggested that the alterations in the proteome were largely induced by the immune response, which invokes the NFkB signaling pathway. The proteomics and computational analysis verified the known functional interplay between inflammation, epilepsy and sleep and highlighted proteins that are involved in their common synaptic mechanisms. Our physiological findings support the phenomenon that high dose of peripheral LPS injection increases SWD-number, modifies its duration as well as the sleep–wake stages and decreases body temperature.},
	year = {2014},
	eissn = {1090-2139},
	pages = {86-95},
	orcid-numbers = {Györffy, Balázs/0000-0003-0654-0641; Kovács, Zsolt/0000-0001-8571-5686; Szabó, Zoltán/0000-0001-8278-8038; Janáky, Tamás/0000-0002-6466-8283; Dobolyi, Árpád/0000-0003-0397-2991; Juhász, Gábor Dénes/0000-0002-0849-6931; Czurkó, András/0000-0002-1985-7296; Kékesi, Adrienna Katalin/0000-0003-3042-4878}
}

@article{MTMT:1223704,
	title = {Facilitation of spike-wave discharge activity by lipopolysaccharides in Wistar Albino Glaxo/Rijswijk rats},
	url = {https://m2.mtmt.hu/api/publication/1223704},
	author = {Kovács, Zsolt and Kékesi, Adrienna Katalin and Szilágyi, Nóra and Ábrahám, István and Székács, D and Király, N and Papp, E and Császár, I and Szegő, Éva Mónika and Barabás, Klaudia and Péterfy, H and Erdei, Anna and Bártfai, T and Juhász, Gábor Dénes},
	doi = {10.1016/j.neuroscience.2006.02.023},
	journal-iso = {NEUROSCIENCE},
	journal = {NEUROSCIENCE},
	volume = {140},
	unique-id = {1223704},
	issn = {0306-4522},
	abstract = {In normal rats the proinflammatory cytokines like interleukin-1?, interleukin-6, which are induced by bacterial lipopolysaccharides, are able to control thalamo-cortical excitability by exerting strong effects on physiological synchronization such as sleep and on pathological synchronization like that in epileptic discharges. To investigate whether proinflammatory cytokines or lipopolysaccharides could modulate absence seizures resulting from a very different generator mechanism than the already investigated bicuculline-, kindling- and kainate-induced seizures, we used a genetically epileptic Wistar Albino Glaxo/Rijswijk rat strain, which is spontaneously generating high voltage spike-wave discharges. Wistar Albino Glaxo/Rijswijk rats responded with an increase of the number of spike-wave discharges to lipopolysaccharide injection (from 10 ?g/kg to 350 ?g/kg). Repetitive administration of 350 ?g/kg lipopolysaccharides daily for 5 days increased the number of spike-wave discharges on the first, second and third days but the number of spike-wave discharges returned to the control value on day 5, at the 5th injection of lipopolysaccharides, showing a tolerance to lipopolysaccharides. The lipopolysaccharide-induced increase in spike-wave discharges was not directly correlated with the elevation of the core body temperature, as it is in febrile seizures, although lipopolysaccharide induced prostaglandin and is clearly pyrogenic at the doses used. Indomethacin, the prostaglandin synthesis inhibitor, efficiently blocked lipopolysaccharide-induced enhancement of spike-wave discharge genesis suggesting that the spike-wave discharge facilitating effect of lipopolysaccharides involves induction of cyclooxygenase 2 and subsequent synthesis and actions of prostaglandin E2. Low dose (40 mg/kg, i.p.) of competitive N-methyl-d-aspartate receptor antagonist 2-amino-5-phosphonopentanoic acid, and low dose of lipopolysaccharide (20 ?g/kg) showed a synergistic interaction to increase the number of spike-wave discharges, whereas at supramaximal doses of lipopolysaccharide and the N-methyl-d-aspartate antagonist no synergy was present. The data reveal a functional connection between absence epileptic activity and lipopolysaccharide induction of prostaglandin synthesis and prostaglandin action and suggest some common cellular targets in epilepsy and lipopolysaccharide-induced inflammation. Š 2006 IBRO.},
	year = {2006},
	eissn = {1873-7544},
	pages = {731-742},
	orcid-numbers = {Kovács, Zsolt/0000-0001-8571-5686; Kékesi, Adrienna Katalin/0000-0003-3042-4878; Erdei, Anna/0000-0002-3622-6680; Juhász, Gábor Dénes/0000-0002-0849-6931}
}