TY  - JOUR
AU  - Rónai, András
AU  - Szemenyei, Erzsébet
AU  - Kató, Erzsébet
AU  - Kocsis, László
AU  - Orosz, György
AU  - Al-Khrasani, Mahmoud
AU  - Tóth, Géza
TI  - Endomorphin synthesis in rat brain from intracerebroventricularly injected [H-3]-Tyr-Pro: A possible biosynthetic route for endomorphins
JF  - REGULATORY PEPTIDES
J2  - REGUL PEPTIDES
VL  - 134
PY  - 2006
IS  - 1
SP  - 54
EP  - 60
PG  - 7
SN  - 0167-0115
DO  - 10.1016/j.regpep.2005.12.004
UR  - https://m2.mtmt.hu/api/publication/1626916
ID  - 1626916
N1  - Cited By :17            
            Export Date: 29 May 2022            
            CODEN: REPPD
AB  - in spite of concentrated efforts, the biosynthetic route of mu-opioid receptor agonist brain tetrapeptide endomorphins (Tyr-Pro-Trp-Phe-NH2 and Tyr-Pro-Phe-Phe-NH2), discovered in 1997, is still obscure. We report presently that 30 min after intracerebroventricular injection of 20 or 200 mu Ci [H-3]Tyr-Pro (49.9 Ci mmol(-1)) the incorporated radioactivity was found in endomorphin-related tetra- and tripeptides in rat brain extracts. As detected by the combination of HPLC with radiodetection, a peak corresponding to endomorphin-2-OH could be identified in two of four extracts of "20 mu Ci" series. Radioactive peaks in position of Tyr, Tyr-Pro, Tyr-Pro-Phe or Tyr-Pro-Trp appeared regularly in both series and also in the "tetrapeptide cluster" constituted by endomorphins and their free carboxylic forms. In one of the four extracts in the "200 mu Ci" series a robust active peak in the position of endomorphin 2 could be detected. Intracerebroventricularly injected 100 mmol, but not 10 or 1000 nmol cold Tyr-Pro (devoid of opioid activity in vitro), caused a naloxone-reversible prolongation of tail-flick latency in rats, peaking between 15 and 30 min. We suggest that Tyr-Pro may serve as a biosynthetic precursor to endomorphin synthesis. (C) 2006 Elsevier B.V. All rights reserved.
LA  - English
DB  - MTMT
ER  - 

TY  - JOUR
AU  - Leitgeb, Balázs
AU  - Szekeres, András
AU  - Tóth, Géza
TI  - Conformational analysis of endomorphin-1 by molecular dynamics methods
JF  - JOURNAL OF PEPTIDE RESEARCH
J2  - J PEPT RES
VL  - 62
PY  - 2003
IS  - 4
SP  - 145
EP  - 157
PG  - 13
SN  - 1397-002X
DO  - 10.1034/j.1399-3011.2003.00084.x
UR  - https://m2.mtmt.hu/api/publication/1117747
ID  - 1117747
LA  - English
DB  - MTMT
ER  - 

TY  - JOUR
AU  - Leitgeb, Balázs
AU  - Ötvös, Ferenc
AU  - Tóth, Géza
TI  - Conformational analysis of endomorphin-2 by molecular dynamics methods
JF  - BIOPOLYMERS
J2  - BIOPOLYMERS
VL  - 68
PY  - 2003
SP  - 497
EP  - 511
PG  - 15
SN  - 0006-3525
DO  - 10.1002/bip.10333
UR  - https://m2.mtmt.hu/api/publication/1912835
ID  - 1912835
LA  - English
DB  - MTMT
ER  -