@article{MTMT:1483052, title = {Tahibacter aquaticus gen. nov., sp. nov., a new gammaproteobacterium isolated from the drinking water supply system of Budapest (Hungary)}, url = {https://m2.mtmt.hu/api/publication/1483052}, author = {Makk, Judit and Homonnay, Zalán Gábor and Kéki, Zsuzsa and Lejtovicz, Z and Márialigeti, Károly and Spröer, C and Schumann, P and M Tóth, Erika}, doi = {10.1016/j.syapm.2010.11.001}, journal-iso = {SYST APPL MICROBIOL}, journal = {SYSTEMATIC AND APPLIED MICROBIOLOGY}, volume = {34}, unique-id = {1483052}, issn = {0723-2020}, year = {2011}, eissn = {1618-0984}, pages = {110-115}, orcid-numbers = {Makk, Judit/0000-0001-5768-7622; Homonnay, Zalán Gábor/0000-0003-2473-1775; Kéki, Zsuzsa/0000-0001-6502-8395; Márialigeti, Károly/0000-0003-1536-5160; M Tóth, Erika/0000-0001-9048-5758} } @article{MTMT:1399428, title = {Nocardioides hungaricus sp. nov., isolated from a drinking water supply system}, url = {https://m2.mtmt.hu/api/publication/1399428}, author = {M Tóth, Erika and Kéki, Zsuzsa and Makk, Judit and Homonnay, Zalán Gábor and Márialigeti, Károly and Schumann, P}, doi = {10.1099/ijs.0.022939-0}, journal-iso = {INT J SYST EVOL MICR}, journal = {INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY}, volume = {61}, unique-id = {1399428}, issn = {1466-5026}, year = {2011}, eissn = {1466-5034}, pages = {549-553}, orcid-numbers = {M Tóth, Erika/0000-0001-9048-5758; Kéki, Zsuzsa/0000-0001-6502-8395; Makk, Judit/0000-0001-5768-7622; Homonnay, Zalán Gábor/0000-0003-2473-1775; Márialigeti, Károly/0000-0003-1536-5160} } @article{MTMT:1394753, title = {Detection of potentially pathogenic bacteria in the drinking water distribution system of a hospital in Hungary}, url = {https://m2.mtmt.hu/api/publication/1394753}, author = {Felföldi, Tamás and Heeger, Z and Vargha, Márta and Márialigeti, Károly}, doi = {10.1111/j.1469-0691.2009.02795.x}, journal-iso = {CLIN MICROBIOL INFEC}, journal = {CLINICAL MICROBIOLOGY AND INFECTION}, volume = {16}, unique-id = {1394753}, issn = {1198-743X}, abstract = {The drinking water distribution system of a hospital was investigated using standard cultivation techniques, taxon-specific PCRs targeting pathogenic bacteria, denaturing gradient gel electrophoresis, cloning and sequencing. The results obtained verify the higher sensitivity of PCR compared to cultivation for detecting Legionella and Pseudomonas aeruginosa. Moreover, several other opportunistic pathogenic bacteria, such as Escherichia albertii, Acinetobacter lwoffi and Corynebacterium tuberculostrearicum, were detected, emphasizing that drinking water systems, especially those with stagnant water sections, could be the source of nosocomial infections.}, keywords = {DNA; POLYMERASE-CHAIN-REACTION; PCR; CULTURE; CYSTIC-FIBROSIS; PSEUDOMONAS-AERUGINOSA; LEGIONELLA-PNEUMOPHILA; RIBOSOMAL-RNA SEQUENCE; waterborne nosocomial infections; Pseudomonas aeruginosa; PCR detection; Legionella; Cloning and sequencing}, year = {2010}, eissn = {1469-0691}, pages = {89-92}, orcid-numbers = {Felföldi, Tamás/0000-0003-2009-2478; Márialigeti, Károly/0000-0003-1536-5160} } @article{MTMT:1394751, title = {Microbial community structure changes during oyster mushroom substrate preparation}, url = {https://m2.mtmt.hu/api/publication/1394751}, author = {Vajna, Balázs and Somosné Nagy, Adrienn and Sajben-Nagy, Enikő Ilona and Manczinger, László and Szijarto, N and Kadar, Z and Bordas, D and Márialigeti, Károly}, doi = {10.1007/s00253-009-2371-3}, journal-iso = {APPL MICROBIOL BIOT}, journal = {APPLIED MICROBIOLOGY AND BIOTECHNOLOGY}, volume = {86}, unique-id = {1394751}, issn = {0175-7598}, abstract = {Although oyster mushroom (Pleurotus spp.) is a valuable food, cultivated worldwide on an industrial scale, still very little is known about the microbial dynamics during oyster mushroom substrate preparation. Therefore, the characterization of the microbial dynamics by chemical and biological tools was the objective of this study. During substrate preparation, enzymatic digestibility of the substrate improved by 77%, whereas the cellulose and hemicellulose to lignin ratios decreased by 9% and 19%, respectively. Fluorescein diacetate hydrolysis reached its minimum value at the temperature maximum of the process during the composting phase and exceeded the initial level at the end of the process. Fungal species played part in the initial mesophilic phase of the substrate preparation process, but they disappeared after pasteurization in tunnels at constant elevated temperatures. Changes in the microbiota showed a marked bacterial community succession during substrate preparation investigated by 16S ribosomal deoxyribonucleic acid-based terminal restriction fragment length polymorphism (T-RFLP). Mature samples represented the least variance, which indicated the effect of the standardized preparation protocol. The relation between mushroom yield and the bacterial community T-RFLP fingerprints was investigated, but the uniformity of mushroom yields did not support any significant correlation.}, keywords = {GENES; DIVERSITY; PCR; PROFILES; COMPOST; RAPID METHOD; AGARICUS-BISPORUS; WHEAT-STRAW; PLEUROTUS-OSTREATUS CULTIVATION; 16S RIBOSOMAL-RNA; Microbial succession; Substrate production; Oyster mushroom}, year = {2010}, eissn = {1432-0614}, pages = {367-375}, orcid-numbers = {Vajna, Balázs/0000-0002-5604-7997; Manczinger, László/0000-0003-1031-7522; Márialigeti, Károly/0000-0003-1536-5160} } @article{MTMT:1394754, title = {DGGE and T-RFLP Analysis of Bacterial Succession during Mushroom Compost Production and Sequence-aided T-RFLP Profile of Mature Compost}, url = {https://m2.mtmt.hu/api/publication/1394754}, author = {Székely, Anna and Sipos, Rita and Berta, B and Vajna, Balázs and Hajdu, C and Márialigeti, Károly}, doi = {10.1007/s00248-008-9424-5}, journal-iso = {MICROB ECOL}, journal = {MICROBIAL ECOLOGY}, volume = {57}, unique-id = {1394754}, issn = {0095-3628}, abstract = {The amount of button mushroom (Agaricus bisporus) harvested from compost is largely affected by the microbial processes taking place during composting and the microbes inhabiting the mature compost. In this study, the microbial changes during the stages of this specific composting process were monitored, and the dominant bacteria of the mature compost were identified to reveal the microbiological background of the favorable properties of the heat-treated phase II mushroom compost. 16S ribosomal deoxyribonucleic acid (rDNA)-based denaturing gradient gel electrophoresis (DGGE) and terminal restriction fragment length polymorphism (T-RFLP) molecular fingerprinting methods were used to track the succession of microbial communities in summer and winter composting cycles. DNA from individual DGGE bands were reamplified and subjected to sequence analysis. Principal component analysis of fingerprints of the composting processes showed intensive changes in bacterial community during the 22-day procedure. Peak temperature samples grouped together and were dominated by Thermus thermophilus. Mature compost patterns were almost identical by both methods (DGGE, T-RFLP). To get an in-depth analysis of the mature compost bacterial community, the sequence data from cultivation of the bacteria and cloning of environmental 16S rDNA were uniquely coupled with the output of the environmental T-RFLP fingerprints (sequence-aided T-RFLP). This method revealed the dominance of a supposedly cellulose-degrading consortium composed of phylotypes related to Pseudoxanthomonas, Thermobifida, and Thermomonospora.}, year = {2009}, eissn = {1432-184X}, pages = {522-533}, orcid-numbers = {Vajna, Balázs/0000-0002-5604-7997; Márialigeti, Károly/0000-0003-1536-5160} }