TY - JOUR AU - Tóth, Emília AU - Fabó, Dániel AU - Entz, László AU - Ulbert, István AU - Erőss, Loránd TI - Intracranial neuronal ensemble recordings and analysis in epilepsy. JF - JOURNAL OF NEUROSCIENCE METHODS J2 - J NEUROSCI METH VL - 260 PY - 2016 IS - SI SP - 261 EP - 269 PG - 9 SN - 0165-0270 DO - 10.1016/j.jneumeth.2015.09.028 UR - https://m2.mtmt.hu/api/publication/3015678 ID - 3015678 N1 - Funding Agency and Grant Number: Hungarian Government - National Research Development and Innovation Office [OTKA PD101754, KTIA NAP 13-1-2013-0001] Funding text: This work was supported by Hungarian Government - National Research Development and Innovation Office (OTKA PD101754, KTIA NAP 13-1-2013-0001). AB - Pathological neuronal firing was demonstrated 50 years ago as the hallmark of epileptically transformed cortex with the use of implanted microelectrodes. Since then, microelectrodes remained only experimental tools in humans to detect unitary neuronal activity to reveal physiological and pathological brain functions. This recording technique has evolved substantially in the past few decades; however, based on recent human data implying their usefulness as diagnostic tools, we expect a substantial increase in the development of microelectrodes in the near future. Here, we review the technological background and history of microelectrode array development for human examinations in epilepsy, including discussions on of wire-based and microelectrode arrays fabricated using micro-electro-mechanical system (MEMS) techniques and novel future techniques to record neuronal ensemble. We give an overview of clinical and surgical considerations, and try to provide a list of probes on the market with their availability for human recording. Then finally, we briefly review the literature on modulation of single neuron for the treatment of epilepsy, and highlight the current topics under examination that can be background for the future development. LA - English DB - MTMT ER - TY - JOUR AU - Keller, CJ AU - Truccolo, W AU - Gale, JT AU - Eskandar, E AU - Thesen, T AU - Carlson, C AU - Devinsky, O AU - Kuzniecky, R AU - Doyle, WK AU - Madsen, JR AU - Schomer, DL AU - Mehta, AD AU - Brown, EN AU - Hochberg, LR AU - Ulbert, István AU - Halgren, E AU - Cash, SS TI - Heterogeneous neuronal firing patterns during interictal epileptiform discharges in the human cortex. JF - BRAIN J2 - BRAIN VL - 133 PY - 2010 IS - Pt 6 SP - 1668 EP - 1681 PG - 14 SN - 0006-8950 DO - 10.1093/brain/awq112 UR - https://m2.mtmt.hu/api/publication/1349493 ID - 1349493 N1 - Megjegyzés-21177851 PN: Part 6 AB - Epileptic cortex is characterized by paroxysmal electrical discharges. Analysis of these interictal discharges typically manifests as spike-wave complexes on electroencephalography, and plays a critical role in diagnosing and treating epilepsy. Despite their fundamental importance, little is known about the neurophysiological mechanisms generating these events in human focal epilepsy. Using three different systems of microelectrodes, we recorded local field potentials and single- unit action potentials during interictal discharges in patients with medically intractable focal epilepsy undergoing diagnostic workup for localization of seizure foci. We studied 336 single units in 20 patients. Ten different cortical areas and the hippocampus, including regions both inside and outside the seizure focus, were sampled. In three of these patients, high density microelectrode arrays simultaneously recorded between 43 and 166 single units from a small (4 mm x 4 mm) patch of cortex. We examined how the firing rates of individual neurons changed during interictal discharges by determining whether the firing rate during the event was the same, above or below a median baseline firing rate estimated from interictal discharge-free periods (Kruskal-Wallis one-way analysis, P<0.05). Only 48% of the recorded units showed such a modulation in firing rate within 500 ms of the discharge. Units modulated during the discharge exhibited significantly higher baseline firing and bursting rates than unmodulated units. As expected, many units (27% of the modulated population) showed an increase in firing rate during the fast segment of the discharge (+/-35 ms from the peak of the discharge), while 50% showed a decrease during the slow wave. Notably, in direct contrast to predictions based on models of a pure paroxysmal depolarizing shift, 7.7% of modulated units recorded in or near the seizure focus showed a decrease in activity well ahead (0-300 ms) of the discharge onset, while 12.2% of units increased in activity in this period. No such pre-discharge changes were seen in regions well outside the seizure focus. In many recordings there was also a decrease in broadband field potential activity during this same pre-discharge period. The different patterns of interictal discharge-modulated firing were classified into more than 15 different categories. This heterogeneity in single unit activity was present within small cortical regions as well as inside and outside the seizure onset zone, suggesting that interictal epileptiform activity in patients with epilepsy is not a simple paroxysm of hypersynchronous excitatory activity, but rather represents an interplay of multiple distinct neuronal types within complex neuronal networks. LA - English DB - MTMT ER - TY - JOUR AU - Cash, SS AU - Halgren, E AU - Dehghani, N AU - Rossetti, AO AU - Thesen, T AU - Wang, C AU - Devinsky, O AU - Kuzniecky, R AU - Doyle, W AU - Madsen, JR AU - Bromfield, E AU - Erőss, Loránd AU - Halász, Péter AU - Karmos, György AU - Csercsa, Richárd AU - Wittner, Lucia AU - Ulbert, István TI - The human K-complex represents an isolated cortical down-state. JF - SCIENCE J2 - SCIENCE VL - 324 PY - 2009 IS - 5930 SP - 1084 EP - 1087 PG - 4 SN - 0036-8075 DO - 10.1126/science.1169626 UR - https://m2.mtmt.hu/api/publication/1234812 ID - 1234812 AB - The electroencephalogram (EEG) is a mainstay of clinical neurology and is tightly correlated with brain function, but the specific currents generating human EEG elements remain poorly specified because of a lack of microphysiological recordings. The largest event in healthy human EEGs is the K- complex (KC), which occurs in slow-wave sleep. Here, we show that KCs are generated in widespread cortical areas by outward dendritic currents in the middle and upper cortical layers, accompanied by decreased broadband EEG power and decreased neuronal firing, which demonstrate a steep decline in network activity. Thus, KCs are isolated "down-states," a fundamental cortico-thalamic processing mode already characterized in animals. This correspondence is compatible with proposed contributions of the KC to sleep preservation and memory consolidation. LA - English DB - MTMT ER - TY - JOUR AU - Csicsvari, J AU - Henze, DA AU - Jamieson, B AU - Harris, KD AU - Sirota, A AU - Barthó, Péter AU - Wise, KD AU - Buzsáki, György TI - Massively parallel recording of unit and local field potentials with silicon-based electrodes JF - JOURNAL OF NEUROPHYSIOLOGY J2 - J NEUROPHYSIOL VL - 90 PY - 2003 SP - 1314 EP - 1323 PG - 10 SN - 0022-3077 DO - 10.1152/jn.00116.2003 UR - https://m2.mtmt.hu/api/publication/109455 ID - 109455 LA - English DB - MTMT ER -