TY - JOUR AU - Takáts, Szabolcs AU - Varga, Ágnes AU - Pircs, Karolina Milena AU - Juhász, Gábor TI - Loss of Drosophila Vps16A enhances autophagosome formation through reduced TOR activity. JF - AUTOPHAGY J2 - AUTOPHAGY VL - 11 PY - 2015 IS - 8 SP - 1209 EP - 1215 PG - 7 SN - 1554-8627 DO - 10.1080/15548627.2015.1059559 UR - https://m2.mtmt.hu/api/publication/2909898 ID - 2909898 N1 - Megjegyzés-25134925 N1 Funding Details: 087518/Z/08/Z, Wellcome Trust Journal Article; Research Support, Non-U.S. Gov't Department of Anatomy, Cell and Developmental Biology, Eötvös Lorand University, Budapest, Hungary Momentum Drosophila Autophagy Research Group, Institute of Genetics, Biological Research Center, Hungarian Academy of Sciences, Szeged, Hungary Molecular Neurogenetics, Lund University, Sölvegatan, Sweden Cited By :6 Export Date: 4 May 2021 Correspondence Address: Juhász, G.; Department of Anatomy, Hungary; email: szmrt@elte.hu Funding Agency and Grant Number: Wellcome TrustWellcome TrustEuropean Commission [087518/Z/08/Z]; Hungarian Scientific Research FundOrszagos Tudomanyos Kutatasi Alapprogramok (OTKA) [OTKA K83509] Funding text: We thank the Wellcome Trust (087518/Z/08/Z) and the Hungarian Scientific Research Fund (OTKA K83509) for support. Department of Anatomy, Cell and Developmental Biology, Eötvös Lorand University, Budapest, Hungary Momentum Drosophila Autophagy Research Group, Institute of Genetics, Biological Research Center, Hungarian Academy of Sciences, Szeged, Hungary Molecular Neurogenetics, Lund University, Sölvegatan, Sweden Cited By :6 Export Date: 23 September 2021 Correspondence Address: Juhász, G.; Department of Anatomy, Hungary; email: szmrt@elte.hu AB - The HOPS tethering complex facilitates autophagosome-lysosome fusion by binding to Syntaxin 17, the autophagosomal SNARE. Here we show that loss of the core HOPS complex subunit Vps16A enhances autophagosome formation and slows down Drosophila development. Mechanistically, Tor kinase is less active in Vps16A mutants likely due to impaired endocytic and biosynthetic transport to the lysosome, a site of its activation. Tor reactivation by overexpression of Rheb suppresses autophagosome formation and restores growth and developmental timing in these animals. Thus, Vps16A reduces autophagosome numbers both by indirectly restricting their formation rate and by directly promoting their clearance. In contrast, the loss of Syx17/Syntaxin 17 blocks autophagic flux without affecting the induction step in Drosophila. LA - English DB - MTMT ER - TY - JOUR AU - Honti, Viktor AU - Csordás, Gábor AU - Kurucz, Judit Éva AU - Márkus, Róbert AU - Andó, István TI - The cell-mediated immunity of Drosophila melanogaster: Hemocyte lineages, immune compartments, microanatomy and regulation. JF - DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY J2 - DEV COMP IMMUNOL VL - 42 PY - 2014 IS - 1 SP - 47 EP - 56 PG - 10 SN - 0145-305X DO - 10.1016/j.dci.2013.06.005 UR - https://m2.mtmt.hu/api/publication/2372553 ID - 2372553 N1 - Cited By :124 Export Date: 19 January 2022 CODEN: DCIMD Correspondence Address: Andó, I.; Institute of Genetics Biological Research Centre of the Hungarian Academy of Sciences, P.O. Box 521, Szeged H-6701, Hungary; email: ando.istvan@brc.mta.hu AB - In the animal kingdom, innate immunity is the first line of defense against invading pathogens. The dangers of microbial and parasitic attacks are countered by similar mechanisms, involving the prototypes of the cell-mediated immune responses, the phagocytosis and encapsulation. Work on Drosophila has played an important role in promoting an understanding of the basic mechanisms of phylogenetically conserved modules of innate immunity. The aim of this review is to survey the developments in the identification and functional definition of immune cell types and the immunological compartments of Drosophila melanogaster. We focus on the molecular and developmental aspects of the blood cell types and compartments, as well as the dynamics of blood cell development and the immune response. Further advances in the characterization of the innate immune mechanisms in Drosophila will provide basic clues to the understanding of the importance of the evolutionary conserved mechanisms of innate immune defenses in the animal kingdom. LA - English DB - MTMT ER - TY - JOUR AU - Nagaosa, K AU - Okada, R AU - Nonaka, S AU - Takeuchi, K AU - Fujita, Y AU - Miyasaka, T AU - Manaka, J AU - Andó, István AU - Nakanishi, Y TI - Integrin beta nu-mediated Phagocytosis of Apoptotic Cells in Drosophila Embryos JF - JOURNAL OF BIOLOGICAL CHEMISTRY J2 - J BIOL CHEM VL - 286 PY - 2011 IS - 29 SP - 25770 EP - 25777 PG - 8 SN - 0021-9258 DO - 10.1074/jbc.M110.204503 UR - https://m2.mtmt.hu/api/publication/1921818 ID - 1921818 N1 - Megjegyzés-22183068 DI: 10.1074/jbc.M110.204503 AB - To identify molecules that play roles in the clearance of apoptotic cells by Drosophila phagocytes, we examined a series of monoclonal antibodies raised against larval hemocytes for effects on phagocytosis in vitro. One antibody that inhibited phagocytosis recognized terribly reduced optic lobes (Trol), a core protein of the perlecan-type proteoglycan, and the level of phagocytosis in embryos of a Trol-lacking fly line was lower than in a control line. The treatment of a hemocyte cell line with a recombinant Trol protein containing the amino acid sequence RGD augmented the phosphorylation of focal adhesion kinase, a hallmark of integrin activation. A loss of integrin beta nu, one of the two beta subunits of Drosophila integrin, brought about a reduction in the level of apoptotic cell clearance in embryos. The presence of integrin beta nu at the surface of embryonic hemocytes was confirmed, and forced expression of integrin beta nu in hemocytes of an integrin beta nu-lacking fly line recovered the defective phenotype of phagocytosis. Finally, the level of phagocytosis in a fly line that lacks both integrin beta nu and Draper, another receptor required for the phagocytosis of apoptotic cells, was lower than that in a fly line lacking either protein. We suggest that integrin beta nu serves as a phagocytosis receptor responsible for the clearance of apoptotic cells in Drosophila, independent of Draper. LA - English DB - MTMT ER - TY - JOUR AU - Somogyi, Kálmán AU - Sipos, Botond AU - Pénzes, Zsolt AU - Kurucz, Judit Éva AU - Zsámboki, János AU - Hultmark, D AU - Andó, István TI - Evolution of Genes and Repeats in the Nimrod Superfamily JF - MOLECULAR BIOLOGY AND EVOLUTION J2 - MOL BIOL EVOL VL - 25 PY - 2008 IS - 11 SP - 2337 EP - 2347 PG - 11 SN - 0737-4038 DO - 10.1093/molbev/msn180 UR - https://m2.mtmt.hu/api/publication/1918046 ID - 1918046 N1 - Megjegyzés-22206057 DI: 10.1093/molbev/msn180 Megjegyzés-22214091 DI: 10.1093/molbev/msn180 AB - The recently identified Nimrod superfamily is characterized by the presence of a special type of EGF repeat, the NIM repeat, located right after a typical CCXGY/W amino acid motif. On the basis of structural features, nimrod genes can be divided into three types. The proteins encoded by Draper-type genes have an EMI domain at the N-terminal part and only one copy of the NIM motif, followed by a variable number of EGF-like repeats. The products of Nimrod B-type and Nimrod C-type genes (including the eater gene) have different kinds of N-terminal domains, and lack EGF-like repeats but contain a variable number of NIM repeats. Draper and Nimrod C-type (but not Nimrod B-type) proteins carry a transmembrane domain. Several members of the superfamily were claimed to function as receptors in phagocytosis and/or binding of bacteria, which indicates an important role in the cellular immunity and the elimination of apoptotic cells. In this paper, the evolution of the Nimrod superfamily is studied with various methods on the level of genes and repeats. A hypothesis is presented in which the NIM repeat, along with the EMI domain, emerged by structural reorganizations at the end of an EGF-like repeat chain, suggesting a mechanism for the formation of novel types of repeats. The analyses revealed diverse evolutionary patterns in the sequences containing multiple NIM repeats. Although in the Nimrod B and Nimrod C proteins show characteristics of independent evolution, many internal NIM repeats in Eater sequences seem to have undergone concerted evolution. An analysis of the nimrod genes has been performed using phylogenetic and other methods and an evolutionary scenario of the origin and diversification of the Nimrod superfamily is proposed. Our study presents an intriguing example how the evolution of multigene families may contribute to the complexity of the innate immune response. LA - English DB - MTMT ER - TY - JOUR AU - Kurucz, Judit Éva AU - Váczi, Balázs AU - Márkus, Róbert AU - Laurinyecz, Barbara AU - Vilmos, Péter AU - Zsámboki, János AU - Csorba, Kinga AU - Gateff, E AU - Hultmark, D AU - Andó, István TI - Definition of Drosophila hemocyte subsets by cell-type specific antigens JF - ACTA BIOLOGICA HUNGARICA (1983-2018) J2 - ACTA BIOL HUNG VL - 58 PY - 2007 IS - Suppl. 1 SP - 95 EP - 111 PG - 17 SN - 0236-5383 DO - 10.1556/ABiol.58.2007.Suppl.8 UR - https://m2.mtmt.hu/api/publication/1915261 ID - 1915261 AB - We analyzed the heterogeneity of Drosophila hemocytes on the basis of the expression of cell-type specific antigens. The antigens characterize distinct subsets which partially overlap with those defined by morphological criteria. Oil the basis of the expression or the lack of expression of blood cell antigens the following hemocyte populations have been defined: crystal cells, plasmalocytes, lamellocytes and precursor cells. The expression of the antigens and thus the different cell types are developmentally regulated. The hemocytes are arranged ill four main compartments: the circulating blood cells, the sessile tissue, the lymph glands and the posterior hematopoietic tissue. Each hemocyte compartment has a specific and characteristic composition of the various cell types. The described markers represent the first successful attempt to define hemocyte lineages by immunological markers in Drosophila and help to define morphologically, functionally, spatially and developmentally distinct subsets of hemocyles. LA - English DB - MTMT ER - TY - JOUR AU - Kurucz, Judit Éva AU - Márkus, Róbert AU - Zsámboki, János AU - Medzihradszky F., Katalin AU - Darula, Zsuzsanna AU - Vilmos, Péter AU - Udvardy, Andor AU - Krausz, Ildikó AU - Lukacsovich, Tamás AU - Gateff, E AU - Zettervall, CJ AU - Hultmark, D AU - Andó, István TI - Nimrod, a Putative Phagocytosis Receptor With Egf Repeats in Drosophila Plasmatocytes JF - CURRENT BIOLOGY J2 - CURR BIOL VL - 17 PY - 2007 IS - 7 SP - 649 EP - 654 PG - 6 SN - 0960-9822 DO - 10.1016/j.cub.2007.02.041 UR - https://m2.mtmt.hu/api/publication/1915010 ID - 1915010 N1 - Megjegyzés-22232309 Z9: 60 DI: 10.1016/j.cub.2007.02.041 LA - English DB - MTMT ER - TY - JOUR AU - Kurucz, Judit Éva AU - Zettervall, CJ AU - Sinka, Rita AU - Vilmos, Péter AU - Pivarcsi, A AU - Ekengren, S AU - Hegedűs, Zoltán AU - Andó, István AU - Hultmark, D TI - Hemese, a hemocyte-specific transmembrane protein, affects the cellular immune response in Drosophila JF - PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA J2 - P NATL ACAD SCI USA VL - 100 PY - 2003 IS - 5 SP - 2622 EP - 2627 PG - 6 SN - 0027-8424 DO - 10.1073/pnas.0436940100 UR - https://m2.mtmt.hu/api/publication/1912851 ID - 1912851 LA - English DB - MTMT ER -