TY - JOUR AU - Kovacs, K AU - Szakács, György AU - Zacchi, G TI - Comparative enzymatic hydrolysis of pretreated spruce by supernatants, whole fermentation broths and washed mycelia of Trichoderma reesei and Trichoderma atroviride JF - BIORESOURCE TECHNOLOGY J2 - BIORESOUR TECHNOL VL - 100 PY - 2009 IS - 3 SP - 1350 EP - 1357 PG - 8 SN - 0960-8524 DO - 10.1016/j.biortech.2008.08.006 UR - https://m2.mtmt.hu/api/publication/2673561 ID - 2673561 AB - Cellulase and beta-glucosidase production on steam pretreated spruce (SPS) with Trichoderma reesei Rut C30, Trichoderma atroviride TUB F-1505 and TUB F-1663 was investigated. The enzymes were compared in term of activity, temperature optima and hydrolytic capacity. The T. atroviride cellulases proved to have lower temperature optima for filter paper activity (FPA) assay (50 degrees C) and for hydrolysis of SPS (40 degrees C) than the Rut C30 enzymes (60 degrees C for FPA and 50 degrees C for hydrolysis). Due to high levels of extracellular beta-glucosidases, the T. atroviride enzyme supernatants hydrolyzed the washed SPS to glucose more efficiently than the enzymes produced by T. reesei. On the other hand, when the whole fermentation broths were used instead of the supernatants, thus the mycelium bound enzymes were also present, the hydrolytic capacity of T. reesei Rut C30 was enhanced by approximately 200%, while an improvement of only 15% was observed in case of the T atroviride isolates. (C) 2008 Elsevier Ltd. All rights reserved. LA - English DB - MTMT ER - TY - JOUR AU - Benko, Z AU - Siika-aho, M AU - Viikari, L AU - Réczey, Istvánné TI - Evaluation of the role of xyloglucanase in the enzymatic hydrolysis of lignocellulosic substrates JF - ENZYME AND MICROBIAL TECHNOLOGY J2 - ENZYME MICROB TECH VL - 43 PY - 2008 IS - 2 SP - 109 EP - 114 PG - 6 SN - 0141-0229 DO - 10.1016/j.enzmictec.2008.03.005 UR - https://m2.mtmt.hu/api/publication/2636798 ID - 2636798 AB - Xyloglucan is one of the major components in the primary cell wall of higher plants. It is intimately associated with cellulose chains, forming the load-bearing network of the cell wall. Enzymatic degradation of the xyloglucan polymer by xyloglucanases could potentially improve the overall hydrolysis of lignocellulosic substrates by enabling cellulases to hydrolyze the cellulose polymer more efficiently due to the increased surface area. In order to evaluate the role of xyloglucan in the enzymatic hydrolysis of lignocelluloses, II different pretreated cellulosic substrates were hydrolyzed using different combinations of purified cellobiohydrolases I and II, endoglucanase II, xyloglucanases of Trichoderma reesei and Aspergillus P-glucosidase. Xyloglucanase activity improved the total hydrolysis of lignocellulosic substrates in general. These results indicate that this enzyme can be used to enhance total hydrolysis, especially in cases when pretreatment of the lignocellulosic substrate is not optimal. (C) 2008 Elsevier Inc. All rights reserved. LA - English DB - MTMT ER - TY - JOUR AU - Borjesson, J AU - Engqvist, M AU - Sipos, Bálint AU - Tjerneld, F TI - Effect of poly(ethylene glycol) on enzymatic hydrolysis and adsorption of cellulase enzymes to pretreated lignocellulose JF - ENZYME AND MICROBIAL TECHNOLOGY J2 - ENZYME MICROB TECH VL - 41 PY - 2007 IS - 1-2 SP - 186 EP - 195 PG - 10 SN - 0141-0229 DO - 10.1016/j.enzmictec.2007.01.003 UR - https://m2.mtmt.hu/api/publication/2655326 ID - 2655326 LA - English DB - MTMT ER -