TY - JOUR AU - Inoue, K AU - Bokor, Nándor AU - Kogure, S AU - Fujii, M AU - Sakai, M TI - Two-point-separation in a sub-micron nonscanning IR super-resolution microscope based on transient fluorescence detected IR spectroscopy JF - OPTICS EXPRESS J2 - OPT EXPRESS VL - 17 PY - 2009 IS - 14 SP - 12013 EP - 12018 PG - 6 SN - 1094-4087 DO - 10.1364/OE.17.012013 UR - https://m2.mtmt.hu/api/publication/2651040 ID - 2651040 AB - IR absorption of chemical species in microscopic objects such as biological cells cannot be measured by conventional IR microscopes, because of their low resolution. To overcome this problem, we developed a novel far-field IR super-resolution microscope employing transient fluorescence detected IR spectroscopy. The resolution of this microscope was shown to be 880 nm by measuring the image of 1 mu m fluorescent beads. Furthermore, it succeeded in resolving beads located 1.4 mu m apart from each other. This is considerably smaller than the diffraction limit of the applied IR light (3.4 mu m). These results suggest the capability of our microscope to study sub-micron targets such as sub-cellular structures of biological cells. (C) 2009 Optical Society of America LA - English DB - MTMT ER - TY - JOUR AU - Bokor, Nándor AU - Iketaki, Y AU - Watanabe, T AU - Fujii, M TI - Investigation of polarization effects for high-numerical-aperture first-order Laguerre-Gaussian beams by 2D scanning with a single fluorescent microbead JF - OPTICS EXPRESS J2 - OPT EXPRESS VL - 13 PY - 2005 IS - 26 SP - 10440 EP - 10447 PG - 8 SN - 1094-4087 DO - 10.1364/OPEX.13.010440 UR - https://m2.mtmt.hu/api/publication/2642787 ID - 2642787 N1 - Department of Physics, Budapest University of Technology and Economics, Budapest 1111, Hungary Olympus Co., Ltd., 2-3 Kuboyama-cho, Hachioji-shi 192-8512, Japan Chemical Resources Laboratory, Tokyo Institute of Technology, Yokohama 226-8503, Japan Cited By :62 Export Date: 11 May 2021 Correspondence Address: Bokor, N.; Department of Physics, Budapest University of Technology and Economics, Budapest 1111, Hungary; email: nandor.bokor@weizmann.ac.il AB - The focal intensity distribution of strongly focused (NA=0.9) first-order Laguerre-Gaussian doughnut beams is investigated experimentally for three different polarizations: linear, and left-handed circular and right-handed circular. The investigations are done by 2-dimensional scanning the focal plane with of a 100nm diameter fluorescent microbead, and measuring the fluorescence signal. The results are shown to be in excellent agreement with theoretical predictions, and demonstrate the superiority of one of the circular polarizations to achieve a sharp dark central spot. (c) 2005 Optical Society of America. LA - English DB - MTMT ER - TY - JOUR AU - Nagy, Attila Tibor AU - Wu, J AU - Berland, KM TI - Observation volumes and gamma factors in two-photon fluorescence fluctuation spectroscopy JF - BIOPHYSICAL JOURNAL J2 - BIOPHYS J VL - 89 PY - 2005 IS - 3 SP - 2077 EP - 2090 PG - 14 SN - 0006-3495 DO - 10.1529/biophysj.104.052779 UR - https://m2.mtmt.hu/api/publication/144529 ID - 144529 LA - English DB - MTMT ER -