@article{MTMT:1176842,
	title = {The effect of catecholamines, acetylcholine and histamine on progesterone release by human granulosa cells in a granulosa cell superfusion system},
	url = {https://m2.mtmt.hu/api/publication/1176842},
	author = {Bódis, József and Koppán, Miklós and Kornya, László and Tinneberg, HR and Török, Attila},
	doi = {10.1080/713603093},
	journal-iso = {GYNECOL ENDOCRINOL},
	journal = {GYNECOLOGICAL ENDOCRINOLOGY},
	volume = {16},
	unique-id = {1176842},
	issn = {0951-3590},
	abstract = {There are experimental data demonstrating the presence and 
		actions of various
		neurotransmitters in the ovary, thus supporting the view that 
		they might play a
		role in intraovarian regulatory mechanisms, although their exact 
		function in the 
		regulation of ovarian hormone secretion is unclear. The 
		objective of the present 
		study was to investigate the direct action of catecholamines, 
		acetylcholine and
		histamine on progesterone secretion of human granulosa cells in 
		a superfused cell
		system. Human granulosa cells were isolated from preovulatory 
		follicular fluid
		using a Percoll gradient centrifugation method. Approximately 2 
		x 10(6) cells
		were mixed with Sephadex G-10 and were transferred into two 
		chambers of the
		superfusion apparatus. The system was perfused with a culture 
		medium and test
		materials were added to the system at a dose of 100 pmol/ml. The 
		progesterone
		concentration of samples was measured using an (125)I 
		radioimmunoassay.
		Administration of epinephrine (adrenaline), norepinephrine 
		(noradrenaline),
		dopamine and histamine had no effect on progesterone release. 
		However,
		acetylcholine produced a significant progesterone release, which 
		could be blocked
		by atropine. The observed effect of acetylcholine on 
		progesterone release of
		superfused human granulosa cells may reflect a physiological 
		role of
		acetylcholine in the regulation of granulosa cell function 
		during the menstrual
		cycle.},
	year = {2002},
	eissn = {1473-0766},
	pages = {259-264}
}