TY - JOUR AU - Feczkó, Tivadar AU - Tóth, Judit AU - Dósa, György AU - Gyenis, János TI - Influence of process conditions on the mean size of PLGA nanoparticles JF - CHEMICAL ENGINEERING AND PROCESSING J2 - CHEM ENG PROCESS VL - 50 PY - 2011 IS - 8 SP - 846 EP - 853 PG - 8 SN - 0255-2701 DO - 10.1016/j.cep.2011.05.006 UR - https://m2.mtmt.hu/api/publication/1671122 ID - 1671122 N1 - Research Institute of Chemical and Process Engineering, Faculty of Information Technology, University of Pannonia, Egyetem u. 2H-8200 Veszprem, Hungary Institute of Materials and Environmental Chemistry, Chemical Research Center, Hungarian Academy of Sciences, Pusztaszeri u. 59-67, H-1025 Budapest, Hungary Department of Mathematics, Faculty of Information Technology, University of Pannonia, Egyetem u. 2H-8200 Veszprem, Hungary Cited By :43 Export Date: 15 November 2019 CODEN: CENPE Correspondence Address: Gyenis, J.; Research Institute of Chemical and Process Engineering, Faculty of Information Technology, University of Pannonia, Egyetem u. 2H-8200 Veszprem, Hungary; email: gyenis@mukki.richem.hu Funding details: -2004-05-0031/3.0 Funding text 1: The authors gratefully acknowledge the financial support of the Hungarian National Office for Research and Technology (Grant No. GVOP-3.1.1.-2004-05-0031/3.0 ), and the support of the Central Trans-Danubian Regional Development Agency provided to develop our particle analysis laboratory (Grant No. BAROSS-5-2007-0006 ). Appendix A WoS:hiba:000295504600016 2019-12-06 05:56 cím nem egyezik LA - English DB - MTMT ER - TY - JOUR AU - Feczkó, Tivadar AU - Tóth, Judit AU - Dósa, György AU - Gyenis, János TI - Optimization of protein encapsulation in PLGA nanoparticles JF - CHEMICAL ENGINEERING AND PROCESSING J2 - CHEM ENG PROCESS VL - 50 PY - 2011 IS - 8 SP - 757 EP - 765 PG - 9 SN - 0255-2701 DO - 10.1016/j.cep.2011.06.008 UR - https://m2.mtmt.hu/api/publication/1671149 ID - 1671149 N1 - Megjegyzés-21721495 : FN Thomson Reuters Web of Knowledge Megjegyzés-21699882 : FN Thomson Reuters Web of Knowledge LA - English DB - MTMT ER - TY - JOUR AU - Biró, Emese AU - Szemesné Németh, Ágnes AU - Feczkó, Tivadar AU - Tóth, Judit AU - Sisak, C AU - Gyenis, János TI - Three-step experimental design to determine the effect of process parameters on the size of chitosan microspheres JF - CHEMICAL ENGINEERING AND PROCESSING J2 - CHEM ENG PROCESS VL - 48 PY - 2009 IS - 3 SP - 771 EP - 779 PG - 9 SN - 0255-2701 DO - 10.1016/j.cep.2008.10.001 UR - https://m2.mtmt.hu/api/publication/1304247 ID - 1304247 N1 - Research Institute of Chemical and Process Engineering, University of Pannonia, Egyetem u. 2, H-8200 Veszprem, Hungary Institute of Materials and Environmental Chemistry, Chemical Research Center, Hungarian Academy of Sciences, Pusztaszeri u. 59-67, H-1025 Budapest, Hungary Cited By :27 Export Date: 21 February 2022 CODEN: CENPE Correspondence Address: Gyenis, J.; Research Institute of Chemical and Process Engineering, Egyetem u. 2, H-8200 Veszprem, Hungary; email: gyenis@mukki.richem.hu Funding details: EU MRTN-CT-2004-512247 Funding details: European Commission, EC Funding text 1: The authors gratefully acknowledge the financial support of the European Commission granted through the “Biopowders” Marie Curie Research Training Network (Project No.: EU MRTN-CT-2004-512247). AB - Investigation was carried out to elucidate the influence of process parameters on the mean particle size of chitosan microspheres produced by water-in-oil (w/o) emulsion crosslinking method. The aqueous chitosan solution was dispersed in the oil phase composed of 40% (v/v) sunflower oil and 60% (v/v) n-hexadecane in the presence of Tween 80 surfactant. The droplets were solidified and hardened by glutaraldehyde crosslinking agent. The particle size of the obtained microspheres was influenced by four independent process variables, such as stirring rate, and the concentrations of chitosan, Tween 80 and glutaraldehyde. To study these effects, 4-factorial 3-level Box-Behnken type experimental design and statistical analysis of the experimental results were carried out in three consecutive steps. First, the 4 independent factors at 3 levels were taken into consideration without repetitions, resulting in 27 scheduled experiments. Second, the design was completed by further experiments to check the effects of some of the variables at five levels both inside and outside the original factor intervals used in the first step. In addition, five repeated experiments at the central points of the four factors were also involved in the second step to determine the pure error. In order to elucidate the effects of process variables on the mean particle size, non-linear multidimensional regression was determined. Third, the less significant effects were neglected resulting in a simplified model equation that proved to be suitable for the prediction of the mean particle size as a function of the studied process parameters. (c) 2008 Elsevier B.V. All rights reserved. LA - English DB - MTMT ER - TY - JOUR AU - Feczkó, Tivadar AU - Tóth, Judit AU - Gyenis, János TI - Comparison of the preparation of PLGA-BSA nano- and microparticles by PVA, poloxamer and PVP JF - COLLOIDS AND SURFACES A : PHYSICOCHEMICAL AND ENGINEERING ASPECTS J2 - COLLOID SURFACE A VL - 319 PY - 2008 SP - 188 EP - 195 PG - 8 SN - 0927-7757 DO - 10.1016/j.colsurfa.2007.07.011 UR - https://m2.mtmt.hu/api/publication/1157826 ID - 1157826 AB - The objective of our study was to prepare nano- and microparticles economically considering some practical parameters such as size and encapsulation efficiency as well as ability of particle recovery. Bovine serum albumin (BSA) model protein was encapsulated by poly(d,l-lactideco- glycolide) (PLGA) using a multiple water-in-oil-in-water emulsion-solvent evaporation technique. The effect of three surfactants: polyvinyl alcohol, poloxamer, and polyvinyl pyrrolidone, used in the outer water phase, on the properties of particles was investigated. The emulsifier/PLGA mass ratio played an important role in the preparation procedure of the particles. This ratio was found to be approximately 1 for polyvinyl alcohol (PVA) if the aim was to formulate nanoparticles with narrow size distribution (<220 nm), high yield and good encapsulation efficiency (>90%). Although, a ratio of 2:1 was sufficient to produce submicron particles by poloxamer with high yield, more than 70% and 90% encapsulation efficiency required minimum 4 and 10 emulsifier/PLGA mass ratio, respectively. Five times more PVA and 10 times more poloxamer than the PLGA mass were necessary to obtain nanoparticles which were easy to redisperse after centrifugation. Microparticles released more BSA than nanoparticles prepared by PVA, however, the situation was reverse with poloxamer. Microparticles formulated by polyvinyl pyrrolidone (PVP) showed the fastest in vitro release. LA - English DB - MTMT ER -