TY - JOUR AU - Gyulai, Gábor AU - Kiss, J AU - Jekkel, Zs AU - Kiss, Erzsébet AU - Heszky, László TI - A selective auxin and cytokinin bioassay based on root and shoot formation in vitro. JF - JOURNAL OF PLANT PHYSIOLOGY J2 - J PLANT PHYSIOL VL - 145 PY - 1995 IS - 3 SP - 379 EP - 382 PG - 4 SN - 0176-1617 UR - https://m2.mtmt.hu/api/publication/1064891 ID - 1064891 AB - A new bioassay for auxins and cytokinins was developed based on de novo organogenesis. Auxin-dependent adventitious roots and cytokinin-dependent adventitious shoots were induced selectively in tobacco leaf disc culture incubated on nutritive-medium for 28 days. All auxins applied alone (indole, naphthalene, and phenoxyacetic acids) initiated roots, and all cytokinins used alone (purine and urea derivatives) initiated shoots at a concentration range of about 10(-9) M-10(-4) M. The bioassay separated the effects of auxins and cytokinins eliminating their synergistic effects. Abscisic acid and gibberellin (GA(3)) had no morphogenic activity. LA - English DB - MTMT ER -