@article{MTMT:1064891, title = {A selective auxin and cytokinin bioassay based on root and shoot formation in vitro.}, url = {https://m2.mtmt.hu/api/publication/1064891}, author = {Gyulai, Gábor and Kiss, J and Jekkel, Zs and Kiss, Erzsébet and Heszky, László}, journal-iso = {J PLANT PHYSIOL}, journal = {JOURNAL OF PLANT PHYSIOLOGY}, volume = {145}, unique-id = {1064891}, issn = {0176-1617}, abstract = {A new bioassay for auxins and cytokinins was developed based on de novo organogenesis. Auxin-dependent adventitious roots and cytokinin-dependent adventitious shoots were induced selectively in tobacco leaf disc culture incubated on nutritive-medium for 28 days. All auxins applied alone (indole, naphthalene, and phenoxyacetic acids) initiated roots, and all cytokinins used alone (purine and urea derivatives) initiated shoots at a concentration range of about 10(-9) M-10(-4) M. The bioassay separated the effects of auxins and cytokinins eliminating their synergistic effects. Abscisic acid and gibberellin (GA(3)) had no morphogenic activity.}, year = {1995}, eissn = {1618-1328}, pages = {379-382} }