TY - JOUR AU - Zsámboki, János AU - Csordás, Gábor AU - Honti, Viktor AU - Pintér, Lajos AU - Bajusz, Izabella AU - Galgóczi, László Norbert AU - Andó, István AU - Kurucz, Judit Éva TI - Drosophila Nimrod proteins bind bacteria JF - CENTRAL EUROPEAN JOURNAL OF BIOLOGY J2 - CENT EUR J BIOL VL - 8 PY - 2013 IS - 7 SP - 633 EP - 645 PG - 13 SN - 1895-104X DO - 10.2478/s11535-013-0183-4 UR - https://m2.mtmt.hu/api/publication/2326115 ID - 2326115 N1 - Institute of Genetics, Biological Research Centre of the Hungarian Academy of Sciences, H-6726 Szeged, Hungary Department of Microbiology, Faculty of Science and Informatics, University of Szeged, H-6726 Szeged, Hungary Cited By :14 Export Date: 19 January 2022 Correspondence Address: Kurucz, É.; Institute of Genetics, , H-6726 Szeged, Hungary; email: kurucz.eva@brc.mta.hu AB - Engulfment of foreign particles by phagocytes is initiated by the engagement of phagocytic receptors. We have previously reported that NimC1 is involved in the phagocytosis of bacteria in Drosophila melanogaster. We have identified a family of genes, the Nimrod gene superfamily, encoding characteristic NIM domain containing structural homologues of NimC1. In this work we studied the bacterium-binding properties of the Nimrod proteins by using a novel immunofluorescencebased flow cytometric assay. This method proved to be highly reproducible and suitable for investigations of the bacteriumbinding capacities of putative phagocytosis receptors. We found that NimC1, NimA, NimB1 and NimB2 bind bacteria significantly but differently. In this respect they are similar to other NIM domain containing receptors Eater and Draper. LA - English DB - MTMT ER - TY - JOUR AU - Nagaosa, K AU - Okada, R AU - Nonaka, S AU - Takeuchi, K AU - Fujita, Y AU - Miyasaka, T AU - Manaka, J AU - Andó, István AU - Nakanishi, Y TI - Integrin beta nu-mediated Phagocytosis of Apoptotic Cells in Drosophila Embryos JF - JOURNAL OF BIOLOGICAL CHEMISTRY J2 - J BIOL CHEM VL - 286 PY - 2011 IS - 29 SP - 25770 EP - 25777 PG - 8 SN - 0021-9258 DO - 10.1074/jbc.M110.204503 UR - https://m2.mtmt.hu/api/publication/1921818 ID - 1921818 N1 - Megjegyzés-22183068 DI: 10.1074/jbc.M110.204503 AB - To identify molecules that play roles in the clearance of apoptotic cells by Drosophila phagocytes, we examined a series of monoclonal antibodies raised against larval hemocytes for effects on phagocytosis in vitro. One antibody that inhibited phagocytosis recognized terribly reduced optic lobes (Trol), a core protein of the perlecan-type proteoglycan, and the level of phagocytosis in embryos of a Trol-lacking fly line was lower than in a control line. The treatment of a hemocyte cell line with a recombinant Trol protein containing the amino acid sequence RGD augmented the phosphorylation of focal adhesion kinase, a hallmark of integrin activation. A loss of integrin beta nu, one of the two beta subunits of Drosophila integrin, brought about a reduction in the level of apoptotic cell clearance in embryos. The presence of integrin beta nu at the surface of embryonic hemocytes was confirmed, and forced expression of integrin beta nu in hemocytes of an integrin beta nu-lacking fly line recovered the defective phenotype of phagocytosis. Finally, the level of phagocytosis in a fly line that lacks both integrin beta nu and Draper, another receptor required for the phagocytosis of apoptotic cells, was lower than that in a fly line lacking either protein. We suggest that integrin beta nu serves as a phagocytosis receptor responsible for the clearance of apoptotic cells in Drosophila, independent of Draper. LA - English DB - MTMT ER - TY - JOUR AU - Honti, Viktor AU - Csordás, Gábor AU - Márkus, Róbert AU - Kurucz, Judit Éva AU - Jankovics, Ferenc AU - Andó, István TI - Cell lineage tracing reveals the plasticity of the hemocyte lineages and of the hematopoietic compartments in drosophila melanogaster JF - MOLECULAR IMMUNOLOGY J2 - MOL IMMUNOL VL - 47 PY - 2010 IS - 11-12 SP - 1997 EP - 2004 PG - 8 SN - 0161-5890 DO - 10.1016/j.molimm.2010.04.017 UR - https://m2.mtmt.hu/api/publication/1921048 ID - 1921048 N1 - Cited By :83 Export Date: 19 January 2022 CODEN: IMCHA Correspondence Address: Andó, I.; Institute of Genetics, Temesvári krt. 62, 6726 Szeged, Csongrád, Hungary; email: ando@brc.hu LA - English DB - MTMT ER - TY - JOUR AU - Honti, Viktor AU - Kurucz, Judit Éva AU - Csordás, Gábor AU - Laurinyecz, Barbara AU - Márkus, Róbert AU - Andó, István TI - In vivo detection of lamellocytes in Drosophila melanogaster JF - IMMUNOLOGY LETTERS J2 - IMMUNOL LETT VL - 126 PY - 2009 IS - 1-2 SP - 83 EP - 84 PG - 2 SN - 0165-2478 DO - 10.1016/j.imlet.2009.08.004 UR - https://m2.mtmt.hu/api/publication/1920812 ID - 1920812 N1 - Cited By :27 Export Date: 30 June 2022 CODEN: IMLED LA - English DB - MTMT ER - TY - JOUR AU - Márkus, Róbert AU - Laurinyecz, Barbara AU - Kurucz, Judit Éva AU - Honti, Viktor AU - Bajusz, Izabella AU - Sipos, Botond AU - Somogyi, Kálmán AU - Kronhamn, J AU - Hultmark, D AU - Andó, István TI - Sessile hemocytes as a hematopoietic compartment in drosophila melanogaster JF - PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA J2 - P NATL ACAD SCI USA VL - 106 PY - 2009 IS - 12 SP - 4805 EP - 4809 PG - 5 SN - 0027-8424 DO - 10.1073/pnas.0801766106 UR - https://m2.mtmt.hu/api/publication/1920757 ID - 1920757 N1 - Cited By :174 Export Date: 30 June 2022 CODEN: PNASA LA - English DB - MTMT ER - TY - JOUR AU - Somogyi, Kálmán AU - Sipos, Botond AU - Pénzes, Zsolt AU - Kurucz, Judit Éva AU - Zsámboki, János AU - Hultmark, D AU - Andó, István TI - Evolution of Genes and Repeats in the Nimrod Superfamily JF - MOLECULAR BIOLOGY AND EVOLUTION J2 - MOL BIOL EVOL VL - 25 PY - 2008 IS - 11 SP - 2337 EP - 2347 PG - 11 SN - 0737-4038 DO - 10.1093/molbev/msn180 UR - https://m2.mtmt.hu/api/publication/1918046 ID - 1918046 N1 - Megjegyzés-22206057 DI: 10.1093/molbev/msn180 Megjegyzés-22214091 DI: 10.1093/molbev/msn180 AB - The recently identified Nimrod superfamily is characterized by the presence of a special type of EGF repeat, the NIM repeat, located right after a typical CCXGY/W amino acid motif. On the basis of structural features, nimrod genes can be divided into three types. The proteins encoded by Draper-type genes have an EMI domain at the N-terminal part and only one copy of the NIM motif, followed by a variable number of EGF-like repeats. The products of Nimrod B-type and Nimrod C-type genes (including the eater gene) have different kinds of N-terminal domains, and lack EGF-like repeats but contain a variable number of NIM repeats. Draper and Nimrod C-type (but not Nimrod B-type) proteins carry a transmembrane domain. Several members of the superfamily were claimed to function as receptors in phagocytosis and/or binding of bacteria, which indicates an important role in the cellular immunity and the elimination of apoptotic cells. In this paper, the evolution of the Nimrod superfamily is studied with various methods on the level of genes and repeats. A hypothesis is presented in which the NIM repeat, along with the EMI domain, emerged by structural reorganizations at the end of an EGF-like repeat chain, suggesting a mechanism for the formation of novel types of repeats. The analyses revealed diverse evolutionary patterns in the sequences containing multiple NIM repeats. Although in the Nimrod B and Nimrod C proteins show characteristics of independent evolution, many internal NIM repeats in Eater sequences seem to have undergone concerted evolution. An analysis of the nimrod genes has been performed using phylogenetic and other methods and an evolutionary scenario of the origin and diversification of the Nimrod superfamily is proposed. Our study presents an intriguing example how the evolution of multigene families may contribute to the complexity of the innate immune response. LA - English DB - MTMT ER - TY - JOUR AU - Kurucz, Judit Éva AU - Váczi, Balázs AU - Márkus, Róbert AU - Laurinyecz, Barbara AU - Vilmos, Péter AU - Zsámboki, János AU - Csorba, Kinga AU - Gateff, E AU - Hultmark, D AU - Andó, István TI - Definition of Drosophila hemocyte subsets by cell-type specific antigens JF - ACTA BIOLOGICA HUNGARICA (1983-2018) J2 - ACTA BIOL HUNG VL - 58 PY - 2007 IS - Suppl. 1 SP - 95 EP - 111 PG - 17 SN - 0236-5383 DO - 10.1556/ABiol.58.2007.Suppl.8 UR - https://m2.mtmt.hu/api/publication/1915261 ID - 1915261 AB - We analyzed the heterogeneity of Drosophila hemocytes on the basis of the expression of cell-type specific antigens. The antigens characterize distinct subsets which partially overlap with those defined by morphological criteria. Oil the basis of the expression or the lack of expression of blood cell antigens the following hemocyte populations have been defined: crystal cells, plasmalocytes, lamellocytes and precursor cells. The expression of the antigens and thus the different cell types are developmentally regulated. The hemocytes are arranged ill four main compartments: the circulating blood cells, the sessile tissue, the lymph glands and the posterior hematopoietic tissue. Each hemocyte compartment has a specific and characteristic composition of the various cell types. The described markers represent the first successful attempt to define hemocyte lineages by immunological markers in Drosophila and help to define morphologically, functionally, spatially and developmentally distinct subsets of hemocyles. LA - English DB - MTMT ER - TY - JOUR AU - Kurucz, Judit Éva AU - Márkus, Róbert AU - Zsámboki, János AU - Medzihradszky F., Katalin AU - Darula, Zsuzsanna AU - Vilmos, Péter AU - Udvardy, Andor AU - Krausz, Ildikó AU - Lukacsovich, Tamás AU - Gateff, E AU - Zettervall, CJ AU - Hultmark, D AU - Andó, István TI - Nimrod, a Putative Phagocytosis Receptor With Egf Repeats in Drosophila Plasmatocytes JF - CURRENT BIOLOGY J2 - CURR BIOL VL - 17 PY - 2007 IS - 7 SP - 649 EP - 654 PG - 6 SN - 0960-9822 DO - 10.1016/j.cub.2007.02.041 UR - https://m2.mtmt.hu/api/publication/1915010 ID - 1915010 N1 - Megjegyzés-22232309 Z9: 60 DI: 10.1016/j.cub.2007.02.041 LA - English DB - MTMT ER - TY - JOUR AU - Rus, Florentina AU - Kurucz, Judit Éva AU - Márkus, Róbert AU - Sinenko, SA AU - Laurinyecz, Barbara AU - Pataki, Andrea Csilla AU - Gausz, János AU - Hegedűs, Zoltán AU - Udvardy, Andor AU - Hultmark, D AU - Andó, István TI - Expression pattern of Filamin-240 in Drosophila blood cells JF - GENE EXPRESSION PATTERNS J2 - GENE EXPR PATTERNS VL - 6 PY - 2006 IS - 8 SP - 928 EP - 934 PG - 7 SN - 1567-133X DO - 10.1016/j.modgep.2006.03.005 UR - https://m2.mtmt.hu/api/publication/1786592 ID - 1786592 N1 - Export Date: 27 January 2024; CODEN: GEPEA LA - English DB - MTMT ER - TY - JOUR AU - Márkus, Róbert AU - Kurucz, Judit Éva AU - Rus, Florentina AU - Andó, István TI - Sterile wounding is a minimal and sufficient trigger for a cellular immune response in Drosophila melanogaster JF - IMMUNOLOGY LETTERS J2 - IMMUNOL LETT VL - 101 PY - 2005 IS - 1 SP - 108 EP - 111 PG - 4 SN - 0165-2478 DO - 10.1016/j.imlet.2005.03.021 UR - https://m2.mtmt.hu/api/publication/1913892 ID - 1913892 LA - English DB - MTMT ER - TY - JOUR AU - Williams, MJ AU - Andó, István AU - Hultmark, D TI - Drosophila melanogaster Rac2 is necessary for a proper cellular immune response JF - GENES TO CELLS J2 - GENES CELLS VL - 10 PY - 2005 SP - 813 EP - 823 PG - 11 SN - 1356-9597 DO - 10.1111/j.1365-2443.2005.00883.x UR - https://m2.mtmt.hu/api/publication/1913913 ID - 1913913 LA - English DB - MTMT ER - TY - JOUR AU - Zettervall, CJ AU - Anderl, I AU - Williams, MJ AU - Palmer, R AU - Kurucz, Judit Éva AU - Andó, István AU - Hultmark, D TI - A directed screen for genes involved in Drosophila blood cell activation JF - PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA J2 - P NATL ACAD SCI USA VL - 101 PY - 2004 IS - 39 SP - 14192 EP - 14197 PG - 6 SN - 0027-8424 DO - 10.1073/pnas.0403789101 UR - https://m2.mtmt.hu/api/publication/1913536 ID - 1913536 LA - English DB - MTMT ER - TY - JOUR AU - Asha, H AU - Nagy, István AU - Kovacs, G AU - Stetson, D AU - Andó, István AU - Dearolf, CR TI - Analysis of Ras-induced overproliferation in Drosophila hemocytes JF - GENETICS J2 - GENETICS VL - 163 PY - 2003 SP - 203 EP - 215 PG - 13 SN - 0016-6731 UR - https://m2.mtmt.hu/api/publication/1912854 ID - 1912854 LA - English DB - MTMT ER - TY - JOUR AU - Kurucz, Judit Éva AU - Zettervall, CJ AU - Sinka, Rita AU - Vilmos, Péter AU - Pivarcsi, A AU - Ekengren, S AU - Hegedűs, Zoltán AU - Andó, István AU - Hultmark, D TI - Hemese, a hemocyte-specific transmembrane protein, affects the cellular immune response in Drosophila JF - PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA J2 - P NATL ACAD SCI USA VL - 100 PY - 2003 IS - 5 SP - 2622 EP - 2627 PG - 6 SN - 0027-8424 DO - 10.1073/pnas.0436940100 UR - https://m2.mtmt.hu/api/publication/1912851 ID - 1912851 LA - English DB - MTMT ER -