TY - JOUR AU - Lőrincz, Andrea AU - Rózsa J., Balázs AU - Katona, Gergely AU - Vizi, E. Szilveszter AU - Tamás, Gábor TI - Differential distribution of NCX1 contributes to spine-dendrite compartmentalization in CA1 pyramidal cells JF - PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA J2 - P NATL ACAD SCI USA VL - 104 PY - 2007 IS - 3 SP - 1033 EP - 1038 PG - 6 SN - 0027-8424 DO - 10.1073/pnas.0605412104 UR - https://m2.mtmt.hu/api/publication/247847 ID - 247847 N1 - Department of Comparative Physiology, University of Szeged, Közép fasor 52, 6726, Szeged, Hungary Institute of Experimental Medicine, Hungarian Academy of Sciences, Szigony u 43, H-1083, Budapest, Hungary Division of Cerebral Structure, National Institute for Physiological Sciences, Okazaki, 444-8585, Japan Cited By :39 Export Date: 11 August 2019 CODEN: PNASA Correspondence Address: Tamás, G.; Department of Comparative Physiology, University of Szeged, Közép fasor 52, 6726, Szeged, Hungary; email: gtamas@bio.u-szeged.hu Chemicals/CAS: Calcium, 7440-70-2; Sodium, 7440-23-5; sodium-calcium exchanger 1; Sodium-Calcium Exchanger Department of Comparative Physiology, University of Szeged, Közép fasor 52, 6726, Szeged, Hungary Institute of Experimental Medicine, Hungarian Academy of Sciences, Szigony u 43, H-1083, Budapest, Hungary Division of Cerebral Structure, National Institute for Physiological Sciences, Okazaki, 444-8585, Japan Cited By :41 Export Date: 23 May 2020 CODEN: PNASA Correspondence Address: Tamás, G.; Department of Comparative Physiology, University of Szeged, Közép fasor 52, 6726, Szeged, Hungary; email: gtamas@bio.u-szeged.hu Chemicals/CAS: Calcium, 7440-70-2; Sodium, 7440-23-5; sodium-calcium exchanger 1; Sodium-Calcium Exchanger Department of Comparative Physiology, University of Szeged, Közép fasor 52, 6726, Szeged, Hungary Institute of Experimental Medicine, Hungarian Academy of Sciences, Szigony u 43, H-1083, Budapest, Hungary Division of Cerebral Structure, National Institute for Physiological Sciences, Okazaki, 444-8585, Japan Cited By :41 Export Date: 30 December 2020 CODEN: PNASA Correspondence Address: Tamás, G.; Department of Comparative Physiology, University of Szeged, Közép fasor 52, 6726, Szeged, Hungary; email: gtamas@bio.u-szeged.hu Chemicals/CAS: Calcium, 7440-70-2; Sodium, 7440-23-5; sodium-calcium exchanger 1; Sodium-Calcium Exchanger Funding details: National Institute of Neurological Disorders and Stroke, NINDS, R29NS035915, R01NS035915, R37NS035915 Department of Comparative Physiology, University of Szeged, Közép fasor 52, 6726, Szeged, Hungary Institute of Experimental Medicine, Hungarian Academy of Sciences, Szigony u 43, H-1083, Budapest, Hungary Division of Cerebral Structure, National Institute for Physiological Sciences, Okazaki, 444-8585, Japan Cited By :41 Export Date: 18 March 2021 CODEN: PNASA Correspondence Address: Tamás, G.; Department of Comparative Physiology, Közép fasor 52, 6726, Szeged, Hungary; email: gtamas@bio.u-szeged.hu Chemicals/CAS: Calcium, 7440-70-2; Sodium, 7440-23-5; sodium-calcium exchanger 1; Sodium-Calcium Exchanger Funding details: National Institute of Neurological Disorders and Stroke, NINDS, R29NS035915 AB - Compartmentalization of Ca(2+) between dendritic spines and shafts is governed by diffusion barriers and a range of Ca(2+) extrusion mechanisms. The distinct contribution of different Ca(2+) clearance systems to Ca(2+) compartmentalization in dendritic spines versus shafts remains elusive. We applied a combination of ultrastructural and functional imaging methods to assess the subcellular distribution and role of NCX1 in rat CA1 pyramidal cells. Quantitative electron microscopic analysis of preembedding immunogold reactions revealed uniform densities of NCX1 along the shafts of apical and basal dendrites, but densities in dendritic shafts were approximately seven times higher than in dendritic spines. In line with these results, two-photon imaging of synaptically activated Ca(2+) transients during NCX blockade showed preferential action localized to the dendritic shafts for NCXs in regulating spine-dendrite coupling. LA - English DB - MTMT ER - TY - JOUR AU - Goldberg, JH AU - Tamás, Gábor AU - Aronov, D AU - Yuste, R TI - Calcium microdomains in aspiny dendrites. JF - NEURON J2 - NEURON VL - 40 PY - 2003 IS - 4 SP - 807 EP - 821 PG - 15 SN - 0896-6273 DO - 10.1016/S0896-6273(03)00714-1 UR - https://m2.mtmt.hu/api/publication/1442913 ID - 1442913 AB - Dendritic spines receive excitatory synapses and serve as calcium compartments, which appear to be necessary for input-specific synaptic plasticity. Dendrites of GABAergic interneurons have few or no spines and thus do not possess a clear morphological basis for synapse-specific compartmentalization. We demonstrate using two-photon calcium imaging that activation of single synapses on aspiny dendrites of neocortical fast spiking (FS) interneurons creates highly localized calcium microdomains, often restricted to less than 1 microm of dendritic space. We confirm using ultrastructural reconstruction of imaged dendrites the absence of any morphological basis for this compartmentalization and show that it is dependent on the fast kinetics of calcium-permeable (CP) AMPA receptors and fast local extrusion via the Na+/Ca2+ exchanger. Because aspiny dendrites throughout the CNS express CP-AMPA receptors, we propose that CP-AMPA receptors mediate a spine-free mechanism of input-specific calcium compartmentalization. LA - English DB - MTMT ER -