TY  - JOUR
AU  - Silhavy, Dániel
AU  - Maliga, Pál
TI  - Mapping of promoters for the nucleus encoded plastid RNA polymerase (NEP) in the iojap maize mutant
JF  - CURRENT GENETICS
J2  - CURR GENET
VL  - 33
PY  - 1998
IS  - 5
SP  - 340
EP  - 344
PG  - 5
SN  - 0172-8083
DO  - 10.1007/s002940050345
UR  - https://m2.mtmt.hu/api/publication/1271158
ID  - 1271158
AB  - Plastid genes of higher plants may be transcribed by the plastid-encoded or the nucleus-encoded plastid RNA polymerases (PEP or NEP). The objective of this study was to identify NEP promoters in maize. To separate the NEP and PEP transcription activity, NEP pro meter mapping was carried out in the iojap maize mutant which lacks the PEP. We report here that atpB, an ATPase subunit gene has promoters for both NEP and PEP, while clpP, a protease subunit gene, and the rpoB operon, encoding three PEP subunit genes, are exclusively transcribed from NEP promoters. The maize NEP promoters share sequence homology around the transcription initiation site, including the ATAGAATA/GAA loose consensus identified for tobacco, suggesting conservation of the NEP transcription machinery between monocots and dicots.
LA  - English
DB  - MTMT
ER  - 

TY  - JOUR
AU  - Sriraman, P
AU  - Silhavy, Dániel
AU  - Maliga, Pál
TI  - Transcription from heterologous rRNA operon promoters in chloroplasts reveals requirement for specific activating factors
JF  - PLANT PHYSIOLOGY
J2  - PLANT PHYSIOL
VL  - 117
PY  - 1998
IS  - 4
SP  - 1495
EP  - 1499
PG  - 5
SN  - 0032-0889
DO  - 10.1104/pp.117.4.1495
UR  - https://m2.mtmt.hu/api/publication/1271156
ID  - 1271156
AB  - The plastid rRNA (rm) operon in chloroplasts of tobacco (Nicotiana tabacum), maize, and pea is transcribed by the plastid-encoded plastid RNA polymerase from a sigma(70)-type promoter (P1). In contrast, the rm operon in spinach (Spinacia oleracea) and mustard chloroplasts is transcribed from the distinct Pc promoter, probably also by the plastid-encoded plastid RNA polymerase. Primer-extension analysis reported here indicates that in Arabidopsis both promoters may be active. To understand promoter selection in the plastid rrn operon in the different species, we have tested transcription from the spinach rrn promoter in transplastomic tobacco and from the tobacco rm promoter in transplastomic Arabidopsis. Our data suggest that transcription of the rm operon depends on species-specific factors that facilitate transcription initiation by the general transcription machinery.
LA  - English
DB  - MTMT
ER  -