TY - JOUR AU - Rózsa J., Balázs AU - Zelles, Tibor AU - Vizi, E. Szilveszter AU - Lendvai, Balázs TI - Distance-dependent scaling of calcium transients evoked by backpropagating spikes and synaptic activity in dendrites of hippocampal interneurons JF - JOURNAL OF NEUROSCIENCE J2 - J NEUROSCI VL - 24 PY - 2004 IS - 3 SP - 661 EP - 670 PG - 10 SN - 0270-6474 DO - 10.1523/JNEUROSCI.3906-03.2004 UR - https://m2.mtmt.hu/api/publication/109502 ID - 109502 N1 - Institute of Experimental Medicine, Hungarian Academy of Sciences, H-1083 Budapest, Hungary Institute of Experimental Medicine, Szigony u. 43, H-1083 Budapest, Hungary Cited By :53 Export Date: 12 March 2020 CODEN: JNRSD Correspondence Address: Vizi, E.S.; Institute of Experimental Medicine, Szigony u. 43, H-1083 Budapest, Hungary; email: esvizi@koki.hu Chemicals/CAS: calcium ion, 14127-61-8; Calcium, 7440-70-2 Institute of Experimental Medicine, Hungarian Academy of Sciences, H-1083 Budapest, Hungary Institute of Experimental Medicine, Szigony u. 43, H-1083 Budapest, Hungary Cited By :53 Export Date: 24 May 2020 CODEN: JNRSD Correspondence Address: Vizi, E.S.; Institute of Experimental Medicine, Szigony u. 43, H-1083 Budapest, Hungary; email: esvizi@koki.hu Chemicals/CAS: calcium ion, 14127-61-8; Calcium, 7440-70-2 Institute of Experimental Medicine, Hungarian Academy of Sciences, H-1083 Budapest, Hungary Institute of Experimental Medicine, Szigony u. 43, H-1083 Budapest, Hungary Cited By :56 Export Date: 1 April 2021 CODEN: JNRSD Correspondence Address: Vizi, E.S.; Institute of Experimental Medicine, Szigony u. 43, H-1083 Budapest, Hungary; email: esvizi@koki.hu Chemicals/CAS: calcium ion, 14127-61-8; Calcium, 7440-70-2 Institute of Experimental Medicine, Hungarian Academy of Sciences, H-1083 Budapest, Hungary Institute of Experimental Medicine, Szigony u. 43, H-1083 Budapest, Hungary Cited By :56 Export Date: 20 April 2021 CODEN: JNRSD Correspondence Address: Vizi, E.S.; Institute of Experimental Medicine, Szigony u. 43, H-1083 Budapest, Hungary; email: esvizi@koki.hu Chemicals/CAS: calcium ion, 14127-61-8; Calcium, 7440-70-2 LA - English DB - MTMT ER - TY - JOUR AU - Goldberg, JH AU - Yuste, R AU - Tamás, Gábor TI - Ca2+ imaging of mouse neocortical interneurone dendrites: contribution of Ca2+-permeable AMPA and NMDA receptors to subthreshold Ca2+dynamics. JF - JOURNAL OF PHYSIOLOGY-LONDON J2 - J PHYSIOL-LONDON VL - 551 PY - 2003 IS - 1 SP - 67 EP - 78 PG - 12 SN - 0022-3751 DO - 10.1113/jphysiol.2003.042598 UR - https://m2.mtmt.hu/api/publication/1442914 ID - 1442914 AB - In this second study, we have combined two-photon calcium imaging with whole-cell recording and anatomic reconstructions to directly characterize synaptically evoked calcium signals in three types of mouse V1 supragranular interneurones: parvalbumin-positive fast spikers (FS), calretinin-positive irregular spikers (IS), and adapting cells (AD). We observed that subthreshold synaptic activation evoked calcium signals locally restricted to individual dendritic compartments. These signals were mediated by NMDA receptors (NMDARs) in AD and IS cells, whereas in FS cells, calcium-permeable AMPA receptors (CP-AMPARs) provided an additional and kinetically distinct influx. Furthermore, even a single, subthreshold synaptic activation evoked a larger dendritic calcium influx than backpropagating action potentials. Our results demonstrate that NMDARs dominate subthreshold calcium dynamics in interneurones and reveal the functional contribution of CP-AMPARs to a specific subclass of cortical interneurone. These data highlight different strategies in dendritic signal processing by distinct classes of interneurones. LA - English DB - MTMT ER - TY - JOUR AU - Goldberg, JH AU - Tamás, Gábor AU - Yuste, R TI - Ca2+ imaging of mouse neocortical interneurone dendrites: Ia-type K+ channels control action potential backpropagation. JF - JOURNAL OF PHYSIOLOGY-LONDON J2 - J PHYSIOL-LONDON VL - 551 PY - 2003 IS - 1 SP - 49 EP - 65 PG - 17 SN - 0022-3751 DO - 10.1113/jphysiol.2003.042580 UR - https://m2.mtmt.hu/api/publication/1442916 ID - 1442916 AB - GABAergic interneurones are essential in cortical processing, yet the functional properties of their dendrites are still poorly understood. In this first study, we combined two-photon calcium imaging with whole-cell recording and anatomical reconstructions to examine the calcium dynamics during action potential (AP) backpropagation in three types of V1 supragranular interneurones: parvalbumin-positive fast spikers (FS), calretinin-positive irregular spikers (IS), and adapting cells (AD). Somatically generated APs actively backpropagated into the dendritic tree and evoked instantaneous calcium accumulations. Although voltage-gated calcium channels were expressed throughout the dendritic arbor, calcium signals during backpropagation of both single APs and AP trains were restricted to proximal dendrites. This spatial control of AP backpropagation was mediated by Ia-type potassium currents and could be mitigated by by previous synaptic activity. Further, we observed supralinear summation of calcium signals in synaptically activated dendritic compartments. Together, these findings indicate that in interneurons, dendritic AP propagation is synaptically regulated. We propose that interneurones have a perisomatic and a distal dendritic functional compartment, with different integrative functions. LA - English DB - MTMT ER - TY - JOUR AU - Goldberg, JH AU - Tamás, Gábor AU - Aronov, D AU - Yuste, R TI - Calcium microdomains in aspiny dendrites. JF - NEURON J2 - NEURON VL - 40 PY - 2003 IS - 4 SP - 807 EP - 821 PG - 15 SN - 0896-6273 DO - 10.1016/S0896-6273(03)00714-1 UR - https://m2.mtmt.hu/api/publication/1442913 ID - 1442913 AB - Dendritic spines receive excitatory synapses and serve as calcium compartments, which appear to be necessary for input-specific synaptic plasticity. Dendrites of GABAergic interneurons have few or no spines and thus do not possess a clear morphological basis for synapse-specific compartmentalization. We demonstrate using two-photon calcium imaging that activation of single synapses on aspiny dendrites of neocortical fast spiking (FS) interneurons creates highly localized calcium microdomains, often restricted to less than 1 microm of dendritic space. We confirm using ultrastructural reconstruction of imaged dendrites the absence of any morphological basis for this compartmentalization and show that it is dependent on the fast kinetics of calcium-permeable (CP) AMPA receptors and fast local extrusion via the Na+/Ca2+ exchanger. Because aspiny dendrites throughout the CNS express CP-AMPA receptors, we propose that CP-AMPA receptors mediate a spine-free mechanism of input-specific calcium compartmentalization. LA - English DB - MTMT ER - TY - JOUR AU - Reyes, A AU - Lujan, R AU - Rozov, A AU - Burnashev, N AU - Somogyi, Péter Pál AU - Sakmann, B TI - Target-cell-specific facilitation and depression in neocortical circuits. JF - NATURE NEUROSCIENCE J2 - NAT NEUROSCI VL - 1 PY - 1998 IS - 4 SP - 279 EP - 285 PG - 7 SN - 1097-6256 UR - https://m2.mtmt.hu/api/publication/2138098 ID - 2138098 N1 - Megjegyzés-22898736 Megjegyzés-21894849 Z9: 326 AB - In neocortical circuits, repetitively active neurons evoke unitary postsynaptic potentials (PSPs) whose peak amplitudes either increase (facilitate) or decrease (depress) progressively. To examine the basis for these different synaptic responses, we made simultaneous recordings from three classes of neurons in cortical layer 2/3. We induced repetitive action potentials in pyramidal cells and recorded the evoked unitary excitatory (E)PSPs in two classes of GABAergic neurons. We observed facilitation of EPSPs in bitufted GABAergic interneurons, many of which expressed somatostatin immunoreactivity. EPSPs recorded from multipolar interneurons, however, showed depression. Some of these neurons were immunopositive for parvalbumin. Unitary inhibitory (I)PSPs evoked by repetitive stimulation of a bitufted neuron also showed a less pronounced but significant difference between the two target neurons. Facilitation and depression involve presynaptic mechanisms, and because a single neuron can express both behaviors simultaneously, we infer that local differences in the molecular structure of presynaptic nerve terminals are induced by retrograde signals from different classes of target neurons. Because bitufted and multipolar neurons both formed reciprocal inhibitory connections with pyramidal cells, the results imply that the balance of activation between two recurrent inhibitory pathways in the neocortex depends on the frequency of action potentials in pyramidal cells. LA - English DB - MTMT ER - TY - JOUR AU - BUHL, EH AU - Halasy, Katalin AU - Somogyi, Péter Pál TI - DIVERSE SOURCES OF HIPPOCAMPAL UNITARY INHIBITORY POSTSYNAPTIC POTENTIALS AND THE NUMBER OF SYNAPTIC RELEASE SITES JF - NATURE J2 - NATURE VL - 368 PY - 1994 IS - 6474 SP - 823 EP - 828 PG - 6 SN - 0028-0836 DO - 10.1038/368823a0 UR - https://m2.mtmt.hu/api/publication/1660995 ID - 1660995 N1 - Medical Research Council, Anatomical Neuropharmacology Unit, Oxford University, Mansfield Road, Oxford 0X1 3TH, United Kingdom Department of Zoology, Jozsef Attila University, Szeged, Hungary Cited By :569 Export Date: 6 April 2021 Correspondence Address: Buhl, E.H.; Medical Research Council, Mansfield Road, Oxford 0X1 3TH, United Kingdom Chemicals/CAS: Receptors, GABA-A AB - Dual intracellular recordings from microscopically identified neurons in the hippocampus reveal that the synaptic terminals of three morphologically distinct types of interneuron act through GABA(A) receptors. Each type of interneuron forms up to 12 synaptic contacts with a postsynaptic principal neuron, but each interneuron innervates a different domain of the surface of the postsynaptic neuron. Different kinetics of the postsynaptic effects, together with the strategic placement of synapses, indicate that these GABAergic interneurons serve distinct functions in the cortical network. LA - English DB - MTMT ER -