TY - JOUR AU - Nagy, István AU - Pivarcsi, Andor AU - Kis, K AU - Koreck, Andrea Ildikó AU - Bodai, László AU - McDowell, A AU - Seltmann, H AU - Patrick, S AU - Zouboulis, CC AU - Kemény, Lajos TI - Propionibacterium acnes and lipopolysaccharide induce the expression of antimicrobial peptides and proinflammatory cytokines/chemokines in human sebocytes JF - MICROBES AND INFECTION J2 - MICROBES INFECT VL - 8 PY - 2006 IS - 8 SP - 2195 EP - 2205 PG - 11 SN - 1286-4579 DO - 10.1016/j.micinf.2006.04.001 UR - https://m2.mtmt.hu/api/publication/1141066 ID - 1141066 N1 - Megjegyzés-26844157 N1 Molecular Sequence Numbers: GENBANK: DQ059328, DQ059329, DQ059330, DQ059331; Megjegyzés-20594093 RP: NOT IN FILE AB - Acne is a common skin disorder of the pilosebaceous unit. In addition to genetic, hormonal and environmental factors, abnormal colonization by Propionibacterium acnes has been implicated in the occurrence of acne via the induction of inflammatory mediators. To gain more insight into the role that sebocytes play in the innate immune response of the skin, particularly in acne, we compared the antimicrobial peptide and proinflammatory cytokine/chemokine expression at mRNA and protein levels, as well as the viability and differentiation of SZ95 sebocytes in response to co-culture with representative isolates of P. acnes type IA and type IB as well as Escherichia coli-derived lipopolysaccharide (LPS). We found that, in vitro, P. acnes type IA and IB isolates and LPS induced human beta-defensin-2 and proinflammatory cytokine/chemokine expression, and influenced sebocyte viability and differentiation. Our results provide evidence that sebocytes are capable of producing proinflammatory cytokines/chemokines and antimicrobial peptides, which may have a role in acne pathogenesis. Furthermore, since P. acnes types IA and IB differentially affect both the differentiation and viability of sebocytes, our data demonstrate that different strains of P. acnes vary in their capacity to stimulate an inflammatory response within the pilosebaceous follicle. (c) 2006 Elsevier SAS. All rights reserved. LA - English DB - MTMT ER - TY - JOUR AU - Nagy, István AU - Pivarcsi, Andor AU - Koreck, Andrea Ildikó AU - Széll, Márta AU - Zsoldiné Urbán, Edit AU - Kemény, Lajos TI - Distinct strains of Propionibacterium acnes induces selective human beta-defensin-2 and interleukin-8 expression in human keratinocytes through Toll-like receptors JF - JOURNAL OF INVESTIGATIVE DERMATOLOGY J2 - J INVEST DERMATOL VL - 124 PY - 2005 IS - 5 SP - 931 EP - 938 PG - 8 SN - 0022-202X DO - 10.1111/j.0022-202X.2005.23705.x UR - https://m2.mtmt.hu/api/publication/247220 ID - 247220 LA - English DB - MTMT ER - TY - JOUR AU - Pivarcsi, Andor AU - Nagy, István AU - Kemény, Lajos TI - Innate immunity in the skin: how keratinocytes fight against pathogens JF - CURRENT IMMUNOLOGY REVIEWS J2 - CURR IMMUNOL REV VL - 1 PY - 2005 IS - 1 SP - 29 EP - 42 PG - 14 SN - 1573-3955 DO - 10.2174/1573395052952941 UR - https://m2.mtmt.hu/api/publication/247203 ID - 247203 N1 - Megjegyzés-22236826 M1: Copyright (C) 2012 American Chemical Society (ACS). All Rights Reserved. CAPLUS AN 2005:229902(Journal; General Review) Megjegyzés-22245819 M1: Copyright (C) 2012 American Chemical Society (ACS). All Rights Reserved. CAPLUS AN 2005:229902(Journal; General Review) Megjegyzés-22266902 M1: Copyright (C) 2012 American Chemical Society (ACS). All Rights Reserved. CAPLUS AN 2005:229902(Journal; General Review) Megjegyzés-22236553 M1: Copyright (C) 2012 American Chemical Society (ACS). All Rights Reserved. CAPLUS AN 2005:229902(Journal; General Review) LA - English DB - MTMT ER - TY - JOUR AU - Pivarcsi, Andor AU - Koreck, Andrea Ildikó AU - Bodai, László AU - Széll, Márta AU - Szeg, CS AU - Belső, Nóra AU - Kenderessy Szabó, Anna AU - Csörgő Sándorné Bata, Zsuzsanna AU - Dobozy, Attila AU - Kemény, Lajos TI - Differentiation-regulated expression of Toll-like receptors 2 and 4 in HaCaT keratinocytes JF - ARCHIVES OF DERMATOLOGICAL RESEARCH J2 - ARCH DERMATOL RES VL - 296 PY - 2004 IS - 3 SP - 120 EP - 124 PG - 5 SN - 0340-3696 DO - 10.1007/s00403-004-0475-2 UR - https://m2.mtmt.hu/api/publication/247206 ID - 247206 N1 - Dept. of Dermatology and Allergology, University of Szeged, Korányi fasor 6., H-6701 Szeged, Hungary Dermatological Research Group, Hungarian Academy of Sciences, Szeged, Hungary Cited By :41 Export Date: 17 April 2022 CODEN: ADMFA Correspondence Address: Pivarcsi, A.; Dept. of Dermatology and Allergology, Korányi fasor 6., H-6701 Szeged, Hungary; email: andor@mail.derma.szote.u-szeged.hu Chemicals/CAS: involucrin, 60108-77-2; toll like receptor 2, 203811-81-8; toll like receptor 4, 203811-83-0 Funding details: Hungarian Scientific Research Fund, OTKA, EU5 QLK4-CT-2001-00366, FKFP 0222/2000, T032498, T042738 Funding text 1: Acknowledgements This work was supported by the OTKA grants T042738, T032498, EU5 QLK4-CT-2001-00366 and FKFP 0222/2000. Márta Széll was supported by the Bolyai Foundation of the Hungarian Academy of Sciences. AB - Toll-like receptors (TLRs) play an important role in the recognition of pathogens in keratinocytes. In this study, we investigated whether the differentiation state of HaCaT keratinocytes correlates with the expression of TLR2 and TLR4 genes. The expression levels of TLR2 and TLR4 in a HaCaT differentiation model system were determined using quantitative real-time RT-PCR (Q-RT-PCR) and flow cytometry. The progression of keratinocyte differentiation was monitored by determining the level of involucrin gene expression using Q-RT-PCR. The expression levels of TLR2 and TLR4 increased with the stage of differentiation and there were strong correlations between the expression level of the involucrin gene and those of the TLR2 gene (r=0.809, P<0.0001) and the TLR4 gene (r=0.568, P<0.02). Increased cell surface expression of TLR2 and TLR4 was also found in differentiated HaCaT keratinocytes by flow cytometric analysis. Our findings suggest that upregulation of TLR expression during differentiation in keratinocytes could be a part of the differentiation process of keratinocytes and could have biological significance in protecting skin against microbes. LA - English DB - MTMT ER - TY - JOUR AU - Koreck, Andrea Ildikó AU - Pivarcsi, A AU - Dobozy, Attila AU - Kemény, Lajos TI - The role of innate immunity in the pathogenesis of acne JF - DERMATOLOGY J2 - DERMATOLOGY VL - 206 PY - 2003 IS - 2 SP - 96 EP - 105 PG - 10 SN - 1018-8665 DO - 10.1159/000068476 UR - https://m2.mtmt.hu/api/publication/105754 ID - 105754 N1 - Cited By :123 Export Date: 3 November 2021 CODEN: DERAE Correspondence Address: Koreck, A.; Dept. of Dermatology and Allergology, Korányi fasor 6, H-6720 Szeged, Hungary; email: koreck@derma.szote.u-szeged.hu Chemicals/CAS: interleukin 8, 114308-91-7; lipid, 66455-18-3; Antigens, CD1; CD1d antigen; Drosophila Proteins; Inflammation Mediators; Membrane Glycoproteins; Receptors, Cell Surface; Toll-Like Receptors AB - Acne is a multifactorial disease of the pilosebaceous follicle. The most significant pathogenetic factors of acne are: abnormal ductal keratinization, increased sebum secretion, abnormalities of the microbial flora and inflammation. The pilosebaceous unit is an immunocompetent organ. Keratinocytes and sebocytes may act as immune cells capable of pathogen recognition and abnormal lipid presentation, and they might have an important role in initiating and perpetuating the activation of both innate and adaptive immune responses. The elements of the skin immune system are involved in the development of both noninflammatory and inflammatory acne lesions. LA - English DB - MTMT ER - TY - JOUR AU - Pivarcsi, Andor AU - Bodai, László AU - Rethi, Bence AU - Kenderessy Szabó, Anna AU - Koreck, Andrea Ildikó AU - Széll, Márta AU - Beer, Z AU - Csörgő Sándorné Bata, Zsuzsanna AU - Magocsi, M AU - Rajnavölgyi, Éva AU - Dobozy, Attila AU - Kemény, Lajos TI - Expression and function of Toll-like receptors 2 and 4 in human keratinocytes JF - INTERNATIONAL IMMUNOLOGY J2 - INT IMMUNOL VL - 15 PY - 2003 IS - 6 SP - 721 EP - 730 PG - 10 SN - 0953-8178 DO - 10.1093/intimm/dxg068 UR - https://m2.mtmt.hu/api/publication/105753 ID - 105753 N1 - Dept. of Dermatology and Allergology, University of Szeged, Korányi fasor 6, 6701 Szeged, Hungary Dermatological Research Group, Hungarian Academy of Sciences, 6701 Szeged, Hungary Department of Immunology, Eotvos Lorand University, 2131 Göd, Hungary Department of Forensic Medicine, University of Szeged, Korányi fasor 6, 6701 Szeged, Hungary Dept. of Isotope/Membrane Diagn., Natl. Inst. of Hematology/Immunology, 1113 Budapest, Hungary Institute of Immunology, Faculty of Medicine, University of Debrecen, 4040 Debrecen, Hungary Cited By :277 Export Date: 3 November 2021 CODEN: INIME Correspondence Address: Pivarcsi, A.; Dept. of Dermatology and Allergology, Korányi fasor 6, 6701 Szeged, Hungary; email: andor@derma.szote.u-szeged.hu Chemicals/CAS: gamma interferon, 82115-62-6; interleukin 8, 114308-91-7; mannan, 51395-96-1, 9036-88-8; peptidoglycan, 9047-10-3; toll like receptor 2, 203811-81-8; toll like receptor 4, 203811-83-0; Adaptor Proteins, Signal Transducing; Antigens, Differentiation; Interferon Type II, 82115-62-6; Interleukin-8; Lipopolysaccharides; Membrane Glycoproteins; MYD88 protein, human; Myeloid Differentiation Factor 88; NF-kappa B; Peptidoglycan; Receptors, Cell Surface; Receptors, Immunologic; TLR2 protein, human; TLR4 protein, human; Toll-Like Receptor 2; Toll-Like Receptor 4; Toll-Like Receptors Funding details: QLK4-CT2001-00366 Funding details: 0222, 1271, 2000-151 3,2 Funding details: Hungarian Scientific Research Fund, OTKA, T 030749, T 032494, T 032496, T 032498 Funding text 1: This work was supported by OTKA (grants T 032496, T 030749, T 032498 and T 032494), FKFP (grants 1271 and 0222) and AKP (grant 2000-151 3,2), and EU5 framework program QLK4-CT2001-00366. M. Sz. was supported by the Bolyai Foundation of the Hungarian Academy of Sciences. AB - Keratinocytes have the ability to kill pathogenic fungi and bacteria by producing antimicrobial substances. Recent studies suggest that microbial components use signaling molecules of the human Toll-like receptor (TLR) family to transduce signals in various cells. Here we provide evidence that keratinocytes express both TLR2 and TLR4 at the mRNA and protein levels, and show that TLR2 and TLR4 are present in the normal human epidermis in vivo and that their expression is regulated by microbial components. The expression of myeloid differentiation protein gene (MyD88), which is involved in the signaling pathway of many TLR, was also demonstrated in keratinocytes. LPS + IFN- gamma increased the expression of TLR2 and TLR4 50- and 5-fold respectively. Treatment of keratinocytes with Candida albicans, mannan, Mycobacterium tuberculosis or LIPS with IFN-gamma resulted in the activation and nuclear translocation of NF- kappaB Inhibition of NF-kappaB blocked the Candida-killing activity of keratinocytes, suggesting that the antimicrobial effect of keratinocytes requires NF-kappaB activation. LPS + IFN-gamma, C. albicans (4 Candida/KC), peptidoglycan (1 mug/ml) or M. tuberculosis extract significantly increased IL-8 gene expression after 3 h of treatment (P < 0.05). The increases over the 0-h level were 15-, 8-, 10.8- and 7-fold, respectively. The microbial compound-induced increase in IL-8 gene expression could be inhibited by anti-TLR2 and anti-TLR4 neutralizing antibodies, suggesting that TLRs are involved in the pathogen- induced expression of this pro-inflammatory cytokine. Our findings stress the importance of the role of keratinocytes as a component of innate immunity. LA - English DB - MTMT ER -