@article{MTMT:1141066, title = {Propionibacterium acnes and lipopolysaccharide induce the expression of antimicrobial peptides and proinflammatory cytokines/chemokines in human sebocytes}, url = {https://m2.mtmt.hu/api/publication/1141066}, author = {Nagy, István and Pivarcsi, Andor and Kis, K and Koreck, Andrea Ildikó and Bodai, László and McDowell, A and Seltmann, H and Patrick, S and Zouboulis, CC and Kemény, Lajos}, doi = {10.1016/j.micinf.2006.04.001}, journal-iso = {MICROBES INFECT}, journal = {MICROBES AND INFECTION}, volume = {8}, unique-id = {1141066}, issn = {1286-4579}, abstract = {Acne is a common skin disorder of the pilosebaceous unit. In addition to genetic, hormonal and environmental factors, abnormal colonization by Propionibacterium acnes has been implicated in the occurrence of acne via the induction of inflammatory mediators. To gain more insight into the role that sebocytes play in the innate immune response of the skin, particularly in acne, we compared the antimicrobial peptide and proinflammatory cytokine/chemokine expression at mRNA and protein levels, as well as the viability and differentiation of SZ95 sebocytes in response to co-culture with representative isolates of P. acnes type IA and type IB as well as Escherichia coli-derived lipopolysaccharide (LPS). We found that, in vitro, P. acnes type IA and IB isolates and LPS induced human beta-defensin-2 and proinflammatory cytokine/chemokine expression, and influenced sebocyte viability and differentiation. Our results provide evidence that sebocytes are capable of producing proinflammatory cytokines/chemokines and antimicrobial peptides, which may have a role in acne pathogenesis. Furthermore, since P. acnes types IA and IB differentially affect both the differentiation and viability of sebocytes, our data demonstrate that different strains of P. acnes vary in their capacity to stimulate an inflammatory response within the pilosebaceous follicle. (c) 2006 Elsevier SAS. All rights reserved.}, year = {2006}, eissn = {1769-714X}, pages = {2195-2205}, orcid-numbers = {Bodai, László/0000-0001-8411-626X; Kemény, Lajos/0000-0002-2119-9501} } @article{MTMT:247220, title = {Distinct strains of Propionibacterium acnes induces selective human beta-defensin-2 and interleukin-8 expression in human keratinocytes through Toll-like receptors}, url = {https://m2.mtmt.hu/api/publication/247220}, author = {Nagy, István and Pivarcsi, Andor and Koreck, Andrea Ildikó and Széll, Márta and Zsoldiné Urbán, Edit and Kemény, Lajos}, doi = {10.1111/j.0022-202X.2005.23705.x}, journal-iso = {J INVEST DERMATOL}, journal = {JOURNAL OF INVESTIGATIVE DERMATOLOGY}, volume = {124}, unique-id = {247220}, issn = {0022-202X}, year = {2005}, eissn = {1523-1747}, pages = {931-938}, orcid-numbers = {Széll, Márta/0000-0002-0730-714X; Zsoldiné Urbán, Edit/0000-0002-9602-7552; Kemény, Lajos/0000-0002-2119-9501} } @article{MTMT:247203, title = {Innate immunity in the skin: how keratinocytes fight against pathogens}, url = {https://m2.mtmt.hu/api/publication/247203}, author = {Pivarcsi, Andor and Nagy, István and Kemény, Lajos}, doi = {10.2174/1573395052952941}, journal-iso = {CURR IMMUNOL REV}, journal = {CURRENT IMMUNOLOGY REVIEWS}, volume = {1}, unique-id = {247203}, issn = {1573-3955}, year = {2005}, pages = {29-42}, orcid-numbers = {Kemény, Lajos/0000-0002-2119-9501} } @article{MTMT:247206, title = {Differentiation-regulated expression of Toll-like receptors 2 and 4 in HaCaT keratinocytes}, url = {https://m2.mtmt.hu/api/publication/247206}, author = {Pivarcsi, Andor and Koreck, Andrea Ildikó and Bodai, László and Széll, Márta and Szeg, CS and Belső, Nóra and Kenderessy Szabó, Anna and Csörgő Sándorné Bata, Zsuzsanna and Dobozy, Attila and Kemény, Lajos}, doi = {10.1007/s00403-004-0475-2}, journal-iso = {ARCH DERMATOL RES}, journal = {ARCHIVES OF DERMATOLOGICAL RESEARCH}, volume = {296}, unique-id = {247206}, issn = {0340-3696}, abstract = {Toll-like receptors (TLRs) play an important role in the recognition of pathogens in keratinocytes. In this study, we investigated whether the differentiation state of HaCaT keratinocytes correlates with the expression of TLR2 and TLR4 genes. The expression levels of TLR2 and TLR4 in a HaCaT differentiation model system were determined using quantitative real-time RT-PCR (Q-RT-PCR) and flow cytometry. The progression of keratinocyte differentiation was monitored by determining the level of involucrin gene expression using Q-RT-PCR. The expression levels of TLR2 and TLR4 increased with the stage of differentiation and there were strong correlations between the expression level of the involucrin gene and those of the TLR2 gene (r=0.809, P<0.0001) and the TLR4 gene (r=0.568, P<0.02). Increased cell surface expression of TLR2 and TLR4 was also found in differentiated HaCaT keratinocytes by flow cytometric analysis. Our findings suggest that upregulation of TLR expression during differentiation in keratinocytes could be a part of the differentiation process of keratinocytes and could have biological significance in protecting skin against microbes.}, year = {2004}, eissn = {1432-069X}, pages = {120-124}, orcid-numbers = {Bodai, László/0000-0001-8411-626X; Széll, Márta/0000-0002-0730-714X; Csörgő Sándorné Bata, Zsuzsanna/0000-0002-3732-1743; Kemény, Lajos/0000-0002-2119-9501} } @article{MTMT:105754, title = {The role of innate immunity in the pathogenesis of acne}, url = {https://m2.mtmt.hu/api/publication/105754}, author = {Koreck, Andrea Ildikó and Pivarcsi, A and Dobozy, Attila and Kemény, Lajos}, doi = {10.1159/000068476}, journal-iso = {DERMATOLOGY}, journal = {DERMATOLOGY}, volume = {206}, unique-id = {105754}, issn = {1018-8665}, abstract = {Acne is a multifactorial disease of the pilosebaceous follicle. The most significant pathogenetic factors of acne are: abnormal ductal keratinization, increased sebum secretion, abnormalities of the microbial flora and inflammation. The pilosebaceous unit is an immunocompetent organ. Keratinocytes and sebocytes may act as immune cells capable of pathogen recognition and abnormal lipid presentation, and they might have an important role in initiating and perpetuating the activation of both innate and adaptive immune responses. The elements of the skin immune system are involved in the development of both noninflammatory and inflammatory acne lesions.}, year = {2003}, eissn = {1421-9832}, pages = {96-105}, orcid-numbers = {Kemény, Lajos/0000-0002-2119-9501} } @article{MTMT:105753, title = {Expression and function of Toll-like receptors 2 and 4 in human keratinocytes}, url = {https://m2.mtmt.hu/api/publication/105753}, author = {Pivarcsi, Andor and Bodai, László and Rethi, Bence and Kenderessy Szabó, Anna and Koreck, Andrea Ildikó and Széll, Márta and Beer, Z and Csörgő Sándorné Bata, Zsuzsanna and Magocsi, M and Rajnavölgyi, Éva and Dobozy, Attila and Kemény, Lajos}, doi = {10.1093/intimm/dxg068}, journal-iso = {INT IMMUNOL}, journal = {INTERNATIONAL IMMUNOLOGY}, volume = {15}, unique-id = {105753}, issn = {0953-8178}, abstract = {Keratinocytes have the ability to kill pathogenic fungi and bacteria by producing antimicrobial substances. Recent studies suggest that microbial components use signaling molecules of the human Toll-like receptor (TLR) family to transduce signals in various cells. Here we provide evidence that keratinocytes express both TLR2 and TLR4 at the mRNA and protein levels, and show that TLR2 and TLR4 are present in the normal human epidermis in vivo and that their expression is regulated by microbial components. The expression of myeloid differentiation protein gene (MyD88), which is involved in the signaling pathway of many TLR, was also demonstrated in keratinocytes. LPS + IFN- gamma increased the expression of TLR2 and TLR4 50- and 5-fold respectively. Treatment of keratinocytes with Candida albicans, mannan, Mycobacterium tuberculosis or LIPS with IFN-gamma resulted in the activation and nuclear translocation of NF- kappaB Inhibition of NF-kappaB blocked the Candida-killing activity of keratinocytes, suggesting that the antimicrobial effect of keratinocytes requires NF-kappaB activation. LPS + IFN-gamma, C. albicans (4 Candida/KC), peptidoglycan (1 mug/ml) or M. tuberculosis extract significantly increased IL-8 gene expression after 3 h of treatment (P < 0.05). The increases over the 0-h level were 15-, 8-, 10.8- and 7-fold, respectively. The microbial compound-induced increase in IL-8 gene expression could be inhibited by anti-TLR2 and anti-TLR4 neutralizing antibodies, suggesting that TLRs are involved in the pathogen- induced expression of this pro-inflammatory cytokine. Our findings stress the importance of the role of keratinocytes as a component of innate immunity.}, year = {2003}, eissn = {1460-2377}, pages = {721-730}, orcid-numbers = {Bodai, László/0000-0001-8411-626X; Széll, Márta/0000-0002-0730-714X; Csörgő Sándorné Bata, Zsuzsanna/0000-0002-3732-1743; Kemény, Lajos/0000-0002-2119-9501} }