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Human GCs were isolated from preovulatory follicular fluid aspirated from\n34 women undergoing in vitro fertilization at the University Women's Hospital of \nTubingen. PG and E2 production was measured in the presence and absence of Me,\npropranolol, LH or FSH using radioimmunoassay. Statistical analysis of the data\nwas performed by ANOVA and Newman-Keuls test. Me stimulated E2 secretion in a\ndose-dependent manner. Propranolol did not cause any change in E2 secretion, and \nwhen given with Me, it only partially blocked but could not entirely prevent E2\noutput. There was no statistically significant effect of Me on PG production when\nMe was administered at concentrations between 10(-4) and 10(-8) M. However, at\n10(-3) M Me significantly suppressed PG output of granulosa cells. LH and FSH\nsignificantly stimulated the secretion of both steroid hormones. Me significantly\nreduced LH- and FSH-induced E2 secretion, as well as LH-stimulated PG output,\nwhile it caused only a slight, yet significant decrease in PG secretion. In the\nsuperfused GC system, FSH and LH resulted in a significant stimulatory effect on \nPG release. Me did not modify the stimulatory effect of FSH on PG, while it\ncaused some delay in LH-stimulated PG release. Propranolol and Me had no\nstimulatory effect on PG release. On the basis of our results we suggest that Me \nhas a direct modulatory effect on basal E2 and gonadotropin-stimulated E2 and PG \nsecretion of human GCs. The observed effect may play a physiological role in the \nregulation of GC function during the menstrual cycle.", "subjects" : [ { "otype" : "Classification", "mtid" : 12215, "link" : "/api/classification/12215", "label" : "Klinikai orvostan", "published" : true, "snippet" : true } ], "digital" : null, "printed" : null, "sourceYear" : 2009, "foreignEdition" : true, "foreignLanguage" : true, "fullPublication" : true, "conferencePublication" : false, "nationalOrigin" : null, "missingAuthor" : false, "oaType" : "NONE", "oaCheckDate" : "2024-03-21", "oaFree" : false, "oaByAuthor" : { "otype" : "Admin", "mtid" : 10045349, "link" : "/api/admin/10045349", "label" : "Fekete Rita (PTE GYTK admin 5)", "familyName" : "Fekete", "givenName" : "Rita", "published" : true, "oldId" : 10045349, "snippet" : true }, "citationCount" : 34, "citationCountUnpublished" : 0, "citationCountWoOther" : 34, "independentCitCountWoOther" : 29, "nationalOriginCitationCount" : 5, "foreignEditionCitationCount" : 32, "doiCitationCount" : 23, "wosCitationCount" : 24, "scopusCitationCount" : 29, "wosScopusCitationCount" : 29, "wosScopusCitationCountWoOther" : 29, "wosScopusIndependentCitationCount" : 24, "wosScopusIndependentCitationCountWoOther" : 24, "independentCitationCount" : 29, "selfCitationCount" : 5, "unhandledCitationCount" : 0, "citingPubCount" : 34, "independentCitingPubCount" : 29, "citingPubCountWoOther" : 34, "independentCitingPubCountWoOther" : 29, "unhandledCitingPubCount" : 0, "citedPubCount" : 4, "citedCount" : 4, "ratings" : [ { "otype" : "SjrRating", "mtid" : 6114723, "link" : "/api/sjrrating/6114723", "label" : "sjr:Q2 (2001) Scopus - Obstetrics and Gynecology GYNECOLOGIC AND OBSTETRIC INVESTIGATION 0378-7346 1423-002X", "listPos" : 52, "rankValue" : 0.49, "type" : "journal", "ratingType" : { "otype" : "RatingType", "mtid" : 10002, "link" : "/api/ratingtype/10002", "label" : "sjr", "code" : "sjr", "published" : true, "snippet" : true }, "subject" : { "otype" : "ClassificationExternal", "mtid" : 2729, "link" : "/api/classificationexternal/2729", "label" : "Scopus - Obstetrics and Gynecology", "published" : true, "oldId" : 2729, "snippet" : true }, "ranking" : "Q2", "calculation" : "DIRECT", "published" : true, "oldId" : 6114723, "snippet" : true } ], "ratingsForSort" : "Q2", "hasCitationDuplums" : false, "userChangeableUntil" : "2015-10-26T08:32:30.000+0000", "publishDate" : "2012-08-27T12:59:28.000+0000", "directInstitutesForSort" : "", "ownerAuthorCount" : 4, "ownerInstituteCount" : 22, "directInstituteCount" : 0, "authorCount" : 5, "contributorCount" : 0, "hasQualityFactor" : true, "link" : "/api/publication/1176835", "label" : "Bodis J et al. 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