TY  - JOUR
AU  - Kiss, Erzsébet
AU  - Kiss, J
AU  - Gyulai, Gábor
AU  - Heszky, László
TI  - A novel method for rapid micropropagation of pineapple.
JF  - HORTSCIENCE
J2  - HORTSCIENCE
VL  - 30
PY  - 1995
IS  - 1
SP  - 127
EP  - 129
PG  - 3
SN  - 0018-5345
UR  - https://m2.mtmt.hu/api/publication/1064892
ID  - 1064892
AB  - A novel micropropagation method for pineapple (Ananas comosus 
L.), based on shoot elongation induced in vitro, was 
demonstrated for two cultivars. Decapitated in vitro plantlets 
were used as explants. Shoot etiolation was induced by placing 
explants in a Murashige and Skoog (MS) medium containing NAA (10 
mu M) and incubating in darkness at 28C for30 to 40 days. The 
mean number of the regenerated etiolated shoots per explant was 
2.6+/-0.29. The etiolated shoots were placed into N6 medium 
supplemented with kinetin or BA (25 or 20 mu M, respectively). 
After 4 to 6 weeks, shoots regenerated along the nodes. The 
highest regeneration rate was 15 and 13 plantlets per node with 
25 mu M kinetin and 20 mu M BA, respectively. Regenerated 
plantlets were rooted on a growth-regulator-free MS medium. 
Residual shoots of the initial explants could be recycled by 
rooting on a growth-regulator-free MS medium. This procedure 
enables the regeneration of several thousand plantlets per year. 
Chemical names used: naphthaleneacetic acid (NAA); benzyladenine 
(BA).
LA  - English
DB  - MTMT
ER  -