TY  - JOUR
AU  - Kurucz, Judit Éva
AU  - Váczi, Balázs
AU  - Márkus, Róbert
AU  - Laurinyecz, Barbara
AU  - Vilmos, Péter
AU  - Zsámboki, János
AU  - Csorba, Kinga
AU  - Gateff, E
AU  - Hultmark, D
AU  - Andó, István
TI  - Definition of Drosophila hemocyte subsets by cell-type specific antigens
JF  - ACTA BIOLOGICA HUNGARICA (1983-2018)
J2  - ACTA BIOL HUNG
VL  - 58
PY  - 2007
IS  - Suppl. 1
SP  - 95
EP  - 111
PG  - 17
SN  - 0236-5383
DO  - 10.1556/ABiol.58.2007.Suppl.8
UR  - https://m2.mtmt.hu/api/publication/1915261
ID  - 1915261
AB  - We analyzed the heterogeneity of Drosophila hemocytes on the basis of the expression of cell-type specific antigens. The antigens characterize distinct subsets which partially overlap with those defined by morphological criteria. Oil the basis of the expression or the lack of expression of blood cell antigens the following hemocyte populations have been defined: crystal cells, plasmalocytes, lamellocytes and precursor cells. The expression of the antigens and thus the different cell types are developmentally regulated. The hemocytes are arranged ill four main compartments: the circulating blood cells, the sessile tissue, the lymph glands and the posterior hematopoietic tissue. Each hemocyte compartment has a specific and characteristic composition of the various cell types. The described markers represent the first successful attempt to define hemocyte lineages by immunological markers in Drosophila and help to define morphologically, functionally, spatially and developmentally distinct subsets of hemocyles.
LA  - English
DB  - MTMT
ER  - 

TY  - JOUR
AU  - Nehme, NT
AU  - Liegeois, S
AU  - Kele, Beatrix
AU  - Giammarinaro, P
AU  - Pradel, E
AU  - Hoffmann, JA
AU  - Ewbank, JJ
AU  - Ferrandon, D
TI  - A model of bacterial intestinal infections in Drosophila melanogaster
JF  - PLOS PATHOGENS
J2  - PLOS PATHOG
VL  - 3
PY  - 2007
IS  - 11
PG  - 16
SN  - 1553-7366
DO  - 10.1371/journal.ppat.0030173
UR  - https://m2.mtmt.hu/api/publication/2505827
ID  - 2505827
N1  - Megjegyzés-22155744
DI: 10.1371/journal.ppat.0030173

AB  - Serratia marcescens is an entomopathogenic bacterium that opportunistically infects a wide range of hosts, including humans. In a model of septic injury, if directly introduced into the body cavity of Drosophila, this pathogen is insensitive to the host's systemic immune response and kills flies in a day. We find that S. marcescens resistance to the Drosophila immune deficiency (imd)-mediated humoral response requires the bacterial lipopolysaccharide O-antigen. If ingested by Drosophila, bacteria cross the gut and penetrate the body cavity. During this passage, the bacteria can be observed within the cells of the intestinal epithelium. In such an oral infection model, the flies succumb to infection only after 6 days. We demonstrate that two complementary host defense mechanisms act together against such food-borne infection: an antimicrobial response in the intestine that is regulated by the imd pathway and phagocytosis by hemocytes of bacteria that have escaped into the hemolymph. Interestingly, bacteria present in the hemolymph elicit a systemic immune response only when phagocytosis is blocked. Our observations support a model wherein peptidoglycan fragments released during bacterial growth activate the imd pathway and do not back a proposed role for phagocytosis in the immune activation of the fat body. Thanks to the genetic tools available in both host and pathogen, the molecular dissection of the interactions between S. marcescens and Drosophila will provide a useful paradigm for deciphering intestinal pathogenesis.
LA  - English
DB  - MTMT
ER  -