@article{MTMT:1176835,
	title = {Influence of melatonin on basal and gonadotropin-stimulated progesterone and estradiol secretion of cultured human granulosa cells and in the superfused granulosa cell system.},
	url = {https://m2.mtmt.hu/api/publication/1176835},
	author = {Bódis, József and Koppán, Miklós and Kornya, László and Tinneberg, HR and Török, Attila},
	doi = {10.1159/000052973},
	journal-iso = {GYNECOL OBSTET INVES},
	journal = {GYNECOLOGIC AND OBSTETRIC INVESTIGATION},
	volume = {52},
	unique-id = {1176835},
	issn = {0378-7346},
	abstract = {The aim of this study was to explore the direct action of melatonin (Me) on basal
		and gonadotropin-stimulated progesterone (PG) and estradiol (E2) secretion of
		human granulosa cells (GCs) cultured in serum-free medium and in a superfused GC 
		system. Human GCs were isolated from preovulatory follicular fluid aspirated from
		34 women undergoing in vitro fertilization at the University Women's Hospital of 
		Tubingen. PG and E2 production was measured in the presence and absence of Me,
		propranolol, LH or FSH using radioimmunoassay. Statistical analysis of the data
		was performed by ANOVA and Newman-Keuls test. Me stimulated E2 secretion in a
		dose-dependent manner. Propranolol did not cause any change in E2 secretion, and 
		when given with Me, it only partially blocked but could not entirely prevent E2
		output. There was no statistically significant effect of Me on PG production when
		Me was administered at concentrations between 10(-4) and 10(-8) M. However, at
		10(-3) M Me significantly suppressed PG output of granulosa cells. LH and FSH
		significantly stimulated the secretion of both steroid hormones. Me significantly
		reduced LH- and FSH-induced E2 secretion, as well as LH-stimulated PG output,
		while it caused only a slight, yet significant decrease in PG secretion. In the
		superfused GC system, FSH and LH resulted in a significant stimulatory effect on 
		PG release. Me did not modify the stimulatory effect of FSH on PG, while it
		caused some delay in LH-stimulated PG release. Propranolol and Me had no
		stimulatory effect on PG release. On the basis of our results we suggest that Me 
		has a direct modulatory effect on basal E2 and gonadotropin-stimulated E2 and PG 
		secretion of human GCs. The observed effect may play a physiological role in the 
		regulation of GC function during the menstrual cycle.},
	year = {2001},
	eissn = {1423-002X},
	pages = {198-202}
}