TY  - JOUR
AU  - Pál, Margit
AU  - Varga, Kata
AU  - Nagy, Olga Mária
AU  - Deák, Péter
TI  - Characterization of the APC10/DOC1 subunit of the anaphase promoting complex in Drosophila melanogaster
JF  - ACTA BIOLOGICA HUNGARICA (1983-2018)
J2  - ACTA BIOL HUNG
VL  - 58
PY  - 2007
IS  - Suppl.1.
SP  - 51
EP  - 64
PG  - 14
SN  - 0236-5383
DO  - 10.1556/ABiol.58.2007.Suppl.5
UR  - https://m2.mtmt.hu/api/publication/1915157
ID  - 1915157
N1  - Megjegyzés-22184851
SU: Suppl. S
LA  - English
DB  - MTMT
ER  - 

TY  - JOUR
AU  - Pál, Margit
AU  - Nagy, Olga Mária
AU  - Ménesi, Dalma
AU  - Udvardy, Andor
AU  - Deák, Péter
TI  - Structurally Related Tpr Subunits Contribute Differently to The Function of The Anaphase-promoting Complex in Drosophila Melanogaster
JF  - JOURNAL OF CELL SCIENCE
J2  - J CELL SCI
VL  - 120
PY  - 2007
IS  - 18
SP  - 3238
EP  - 3248
PG  - 11
SN  - 0021-9533
DO  - 10.1242/jcs.004762
UR  - https://m2.mtmt.hu/api/publication/1915149
ID  - 1915149
N1  - Megjegyzés-22232559
Z9: 9
DI: 10.1242/jcs.004762
AB  - The anaphase-promoting complex/cyclosome or APC/C is a key regulator of chromosome segregation and mitotic exit in eukaryotes. It contains at least 11 subunits, most of which are evolutionarily conserved. The most abundant constituents of the vertebrate APC/C are the four structurally related tetratrico-peptide repeat (TPR) subunits, the functions of which are not yet precisely understood. Orthologues of three of the TPR subunits have been identified in Drosophila. We have shown previously that one of the TPR subunits of the Drosophila APC/C, Apc3 (also known as Cdc27 or Makos), is essential for development, and perturbation of its function results in mitotic cyclin accumulation and metaphase-like arrest. In this study we demonstrate that the Drosophila APC/C associates with a new TPR protein, a genuine orthologue of the vertebrate Apc7 subunit that is not found in yeasts. In addition to this, transgenic flies knocked down for three of the TPR genes Apc6 (Cdc16), Apc7 and Apc8 (Cdc23), by RNA interference were established to investigate their function. Whole-body expression of subunit-specific dsRNA efficiently silences these genes resulting in only residual mRNA concentrations. Apc6/Cdc16 and Apc8/Cdc23 silencing induces developmental delay and causes different pupal lethality. Cytological examination showed that these animals had an elevated level of apoptosis, high mitotic index and delayed or blocked mitosis in a prometaphase-metaphase-like state with overcondensed chromosomes. The arrested neuroblasts contained elevated levels of cyclin B but, surprisingly, cyclin A appeared to be degraded normally. Contrary to the situation for the Apc6/Cdc16 and Apc8/Cdc23 genes, the apparent loss of Apc7 function does not lead to the above abnormalities. Instead, the Apc7 knocked down animals and null mutants are viable and fertile, although they display mild chromosome segregation defects and anaphase delay. Nevertheless, the Apc7 subunit shows synergistic genetic interaction with Apc8/Cdc23 that, together with the phenotypic data, assumes a limited functional role for Apc7. Taken together, these data suggest that the structurally related TPR subunits contribute differently to the function of the anaphase-promoting complex.
LA  - English
DB  - MTMT
ER  - 

TY  - JOUR
AU  - Máthé, Endre
AU  - Kraft, C
AU  - Giet, F
AU  - Deák, Péter
AU  - Peters, JM
AU  - Glover, DM
TI  - The E2-C vihar is required for the correct spatiotemporal proteolysis of cyclin B and itself undergoes cyclical degradation
JF  - CURRENT BIOLOGY
J2  - CURR BIOL
VL  - 14
PY  - 2004
SP  - 1723
EP  - 1733
PG  - 11
SN  - 0960-9822
DO  - 10.1016/j.cub.2004.09.023
UR  - https://m2.mtmt.hu/api/publication/1913542
ID  - 1913542
LA  - English
DB  - MTMT
ER  - 

TY  - JOUR
AU  - Capron, A
AU  - Serralbo, O
AU  - Fülöp, Katalin
AU  - Frugier, F
AU  - Parmentier, Y
AU  - Dong, AW
AU  - Lecureuil, A
AU  - Guerche, P
AU  - Kondorosi, Éva
AU  - Scheres, B
AU  - Genschik, P
TI  - The arabidopsis anaphase-promoting complex or cyclosome: molecular and genetic characterization of the APC2 subunit
JF  - PLANT CELL
J2  - PLANT CELL
VL  - 15
PY  - 2003
SP  - 2370
EP  - 2382
PG  - 13
SN  - 1040-4651
DO  - 10.1105/tpc.013847
UR  - https://m2.mtmt.hu/api/publication/1912970
ID  - 1912970
LA  - English
DB  - MTMT
ER  - 

TY  - JOUR
AU  - Deák, Péter
AU  - Donaldson, M
AU  - Glover, DM
TI  - Mutations in makos, a Drosophila gene encoding the Cdc27 subunit of the anaphase promoting complex, enhance centrosomal defects in polo and are suppressed by mutations in twins/aar, which encodes a regulatory subunit of PP2A
JF  - JOURNAL OF CELL SCIENCE
J2  - J CELL SCI
VL  - 116
PY  - 2003
SP  - 4147
EP  - 4158
PG  - 12
SN  - 0021-9533
DO  - 10.1242/jcs.00722
UR  - https://m2.mtmt.hu/api/publication/1912977
ID  - 1912977
LA  - English
DB  - MTMT
ER  -