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gene have been extensively analyzed in different cancers, but no data
ate available for primary malignant melanoma. The aim of the present
study was to simultaneously investigate the EGFR gene and chromosome 7
copy number alterations in 81 cutaneous malignant melanomas by
interphase FISH and correlate the data with clinicopathological
parameters of patients. EGFR mRNA levels were detected by Affymetrix
GeneChip Human Genome U133 Plus 2.0 expression arrays for 16 lesions.
Both increased gene dosage and chromosome 7 alterations were found in
70% of tumors. Extra EGFR copies were detected in an additional 10% of
samples. Polysomy 7 was associated with EGFR gene amplification.
Significant correlation was found between EGFR alterations and
histological subtypes, tumor thickness, ulceration and metastases
formation. Amplification was significantly higher in lesions that
developed metastases within 2 years after surgical excision of the
primary tumor. Gene copy alterations were associated with elevated mRNA
expression in 77% of lesions when compared to tumors with disomic EGFR
status, the correlation was not directly proportional to gene copy
number. Associations between protein expression and mRNA levels were
even less prominent. In conclusion, our study indicates that
amplification of the EGFR gene and polysomy 7 are frequent alterations
in primary melanomas and are associated with bad prognosis. Further
studies are required to clarify whether melanoma patients with EGFR
alterations can benefit from anti-EGFR therapy. (C) 2007 Wiley-Liss,
Inc.</abstractText>
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&lt;span class=&quot;authorAux-mtmt&quot;&gt; Célzott daganatterápia&lt;/span&gt;) &lt;/a&gt; &lt;/span&gt; &lt;span class=&quot;author-affil&quot;&gt;&lt;span title=&quot;Debreceni Egyetem&quot;&gt;DE&lt;/span&gt;/Gyógyszerésztudományi Kar&lt;/span&gt; ;&amp;nbsp;&amp;nbsp;&amp;nbsp; &lt;span class=&quot;author-name&quot; &gt;Begany A &lt;/span&gt; ;&amp;nbsp;&amp;nbsp;&amp;nbsp; &lt;span class=&quot;author-name&quot; mtid=&quot;10004669&quot;&gt;&lt;a href=&quot;/gui2/?type=authors&amp;mode=browse&amp;sel=10004669&quot; target=&quot;_blank&quot;&gt;Hunyadi J (&lt;span class=&quot;authorship-author-name&quot;&gt;Hunyadi János&lt;/span&gt; &lt;span class=&quot;authorAux-mtmt&quot;&gt; Dermatoimmunológia&lt;/span&gt;) &lt;/a&gt; &lt;/span&gt; &lt;span class=&quot;author-affil&quot;&gt;&lt;span title=&quot;Debreceni Egyetem&quot;&gt;DE&lt;/span&gt;/&lt;span title=&quot;Általános Orvostudományi Kar&quot;&gt;ÁOK&lt;/span&gt;/Bőrgyógyászati Tanszék&lt;/span&gt; ;&amp;nbsp;&amp;nbsp;&amp;nbsp; 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href=&quot;https://portal.issn.org/resource/ISSN/0196-4763&quot;&gt;0196-4763&lt;/a&gt; &lt;a target=&quot;_blank&quot; href=&quot;https://portal.issn.org/resource/ISSN/1097-0320&quot;&gt;1097-0320&lt;/a&gt;)&lt;/span&gt;: &lt;span class=&quot;journal-volume&quot;&gt;46&lt;/span&gt; &lt;span class=&quot;journal-issue&quot;&gt;4&lt;/span&gt; &lt;span class=&quot;page&quot;&gt; pp 222-232 &lt;/span&gt; &lt;span class=&quot;year&quot;&gt;(2001)&lt;/span&gt; &lt;/div&gt; &lt;div class=&quot;pub-footer&quot;&gt;  &lt;span class=&quot;language&quot; xmlns=&quot;http://www.w3.org/1999/html&quot;&gt;Nyelv: Angol | &lt;/span&gt; &lt;span class=&quot;identifiers&quot;&gt; &lt;span class=&quot;id identifier oa_none&quot; title=&quot;none&quot;&gt; &lt;a style=&quot;color:black&quot; title=&quot;10.1002/cyto.1131&quot; target=&quot;_blank&quot; href=&quot;https://doi.org/10.1002/cyto.1131&quot;&gt; DOI &lt;/a&gt; &lt;/span&gt; &lt;span class=&quot;id identifier oa_none&quot; title=&quot;none&quot;&gt; &lt;a style=&quot;color:blue&quot; title=&quot;000170758200002&quot; target=&quot;_blank&quot; href=&quot;https://www.webofscience.com/wos/woscc/full-record/000170758200002&quot;&gt; WoS &lt;/a&gt; &lt;/span&gt; &lt;span class=&quot;id identifier oa_CLOSED&quot; title=&quot; Zárt &quot;&gt; &lt;a style=&quot;color:blue&quot; title=&quot;154803&quot; target=&quot;_blank&quot; href=&quot;http://hdl.handle.net/2437/154803&quot;&gt; DEA &lt;/a&gt; &lt;/span&gt; &lt;span class=&quot;id identifier oa_none&quot; title=&quot;none&quot;&gt; &lt;a style=&quot;color:black&quot; title=&quot;0035880481&quot; target=&quot;_blank&quot; href=&quot;http://www.scopus.com/record/display.url?origin=inward&amp;eid=2-s2.0-0035880481&quot;&gt; Scopus &lt;/a&gt; &lt;/span&gt; &lt;span class=&quot;id identifier oa_none&quot; title=&quot;none&quot;&gt; &lt;a style=&quot;color:black&quot; title=&quot;11514955&quot; target=&quot;_blank&quot; href=&quot;http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&amp;db=PubMed&amp;list_uids=11514955&amp;dopt=Abstract&quot;&gt; PubMed &lt;/a&gt; &lt;/span&gt; &lt;/span&gt; &lt;OnlyViewableByAuthor&gt;&lt;div class=&quot;ratings&quot;&gt; &lt;div class=&quot;journal-subject&quot;&gt;Folyóirat szakterülete: Scopus - Endocrinology&amp;nbsp;&amp;nbsp;&amp;nbsp;SJR indikátor:&amp;nbsp;Q1&lt;/div&gt; &lt;div class=&quot;journal-subject&quot;&gt;Folyóirat szakterülete: Scopus - Hematology&amp;nbsp;&amp;nbsp;&amp;nbsp;SJR indikátor:&amp;nbsp;Q1&lt;/div&gt; &lt;div class=&quot;journal-subject&quot;&gt;Folyóirat szakterülete: Scopus - Pathology and Forensic Medicine&amp;nbsp;&amp;nbsp;&amp;nbsp;SJR indikátor:&amp;nbsp;Q1&lt;/div&gt; &lt;div class=&quot;journal-subject&quot;&gt;Folyóirat szakterülete: Scopus - Biophysics&amp;nbsp;&amp;nbsp;&amp;nbsp;SJR indikátor:&amp;nbsp;Q2&lt;/div&gt; &lt;div class=&quot;journal-subject&quot;&gt;Folyóirat szakterülete: Scopus - Cell Biology&amp;nbsp;&amp;nbsp;&amp;nbsp;SJR indikátor:&amp;nbsp;Q2&lt;/div&gt; &lt;/div&gt;&lt;/OnlyViewableByAuthor&gt; &lt;div class=&quot;publication-citation&quot; style=&quot;margin-left: 0.5cm;&quot;&gt; &lt;span title=&quot;Nyilvános idézőközlemények összesen, említések nélkül&quot; class=&quot;citingPub-count&quot;&gt;Nyilvános idéző összesen: 92&lt;/span&gt; | Független: 83 | Függő: 9 | Nem jelölt: 0 | WoS jelölt: 84 | Scopus jelölt:&amp;nbsp;88 | WoS/Scopus jelölt:&amp;nbsp;92 | DOI jelölt:&amp;nbsp;87 &lt;/div&gt;   &lt;div class=&quot;publication-citation&quot;&gt; &lt;a target=&quot;_blank&quot; href=&quot;/api/publication?cond=citations.related;eq;5171&amp;sort=publishedYear,desc&amp;sort=title&quot;&gt; Idézett közlemények száma: 4 &lt;/a&gt; &lt;/div&gt; &lt;div class=&quot;mtid&quot;&gt;&lt;span class=&quot;long-pub-mtid&quot;&gt;Közlemény: 5171&lt;/span&gt; | &lt;span class=&quot;status-data status-ADMIN_APPROVED&quot;&gt; Admin láttamozott &lt;/span&gt; &lt;span class=&quot;oldId&quot;&gt;Régi azonosító: 5171&lt;/span&gt; | Forrás Idéző | &lt;span class=&quot;type-subtype&quot;&gt;Folyóiratcikk ( Szakcikk ) &lt;/span&gt; | &lt;span class=&quot;pub-category&quot;&gt;Tudományos&lt;/span&gt; | &lt;span class=&quot;publication-sourceOfData&quot;&gt;WOS&lt;/span&gt; &lt;/div&gt; &lt;div class=&quot;lastModified&quot;&gt;Utolsó módosítás: 2020.06.09. 09:33 Gyuró Gabriella (DE/ÁOK/BorgyogyT admin 5) &lt;/div&gt; &lt;/div&gt;&lt;/div&gt;</template2>
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